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  1. Article ; Online: Optimizing lyophilization primary Drying: A vaccine case study with experimental and modeling techniques.

    Najarian, Jeff / Metsi-Guckel, Efimia / Renawala, Harshil K / Grosse, Don / Sims, Alexander / Walter, Amanda / Sarkar, Avik / Karande, Atul

    International journal of pharmaceutics

    2024  , Page(s) 124168

    Abstract: In this study, we present the lyophilization process development efforts for a vaccine formulation aimed at optimizing the primary drying time (hence, the total cycle length) through comprehensive evaluation of its thermal characteristics, temperature ... ...

    Abstract In this study, we present the lyophilization process development efforts for a vaccine formulation aimed at optimizing the primary drying time (hence, the total cycle length) through comprehensive evaluation of its thermal characteristics, temperature profile, and critical quality attributes (CQAs). Differential scanning calorimetry (DSC) and freeze-drying microscopy (FDM) were used to experimentally determine the product-critical temperatures, viz., the glass transition temperature (T
    Language English
    Publishing date 2024-04-23
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 428962-6
    ISSN 1873-3476 ; 0378-5173
    ISSN (online) 1873-3476
    ISSN 0378-5173
    DOI 10.1016/j.ijpharm.2024.124168
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1409: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article: Cadherin-11 controls otolith assembly: evidence for extracellular cadherin activity.

    Clendenon, Sherry G / Shah, Bijal / Miller, Caroline A / Schmeisser, Glen / Walter, Amanda / Gattone, Vincent H / Barald, Kate F / Liu, Qin / Marrs, James A

    Developmental dynamics : an official publication of the American Association of Anatomists

    2009  Volume 238, Issue 8, Page(s) 1909–1922

    Abstract: Cadherin-11/Cdh11 is expressed through early development and strongly during inner ear development (otic placode and vesicle). Here we show that antisense knockdown of Cdh11 during early zebrafish development interferes with otolith formation. ... ...

    Abstract Cadherin-11/Cdh11 is expressed through early development and strongly during inner ear development (otic placode and vesicle). Here we show that antisense knockdown of Cdh11 during early zebrafish development interferes with otolith formation. Immunofluorescence labeling showed that Cdh11 expression was concentrated on and within the otolith. Cdh11 was faintly detected at the lateral surface (sites of cell-cell contact) of otic epithelial cells and in the cytoplasm. Strongly labeled Cdh11 containing puncta were detected within the otolymph (the fluid within the otic vesicle) and associated with the otolith surface. BODIPY-ceramine-labeled vesicular structures detected in the otolymph were larger and more numerous in Cdh11 knockdown embryos. We present evidence supporting a working model that vesicular structures containing Cdh11 (perhaps containing biomineralization components) are exported from the otic epithelium into the otolymph, adhere to one another and to the surface of the growing otolith, facilitating otolith growth.
    MeSH term(s) Amino Acid Sequence ; Animals ; Base Sequence ; Cadherins/deficiency ; Cadherins/genetics ; Cadherins/metabolism ; Extracellular Space/metabolism ; Gene Expression Regulation, Developmental ; Gene Targeting ; Models, Biological ; Molecular Sequence Data ; Otolithic Membrane/embryology ; Otolithic Membrane/metabolism ; Phenotype ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Rhombencephalon/abnormalities ; Zebrafish/embryology ; Zebrafish/genetics ; Zebrafish/metabolism ; Zebrafish Proteins/deficiency ; Zebrafish Proteins/genetics ; Zebrafish Proteins/metabolism
    Chemical Substances Cadherins ; RNA, Messenger ; Zebrafish Proteins ; osteoblast cadherin (156621-71-5)
    Language English
    Publishing date 2009-07-07
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1102541-4
    ISSN 1097-0177 ; 1058-8388
    ISSN (online) 1097-0177
    ISSN 1058-8388
    DOI 10.1002/dvdy.22015
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    Ub I Zs.60: Show issues Location:
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    Zs.MG 64: Show issues

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  3. Article ; Online: Thrice weekly azacitidine does not improve hematological responses in lower-risk myelodysplastic syndromes: a study of the Hoosier Oncology Group.

    Sayar, Hamid / Chan, Rebecca J / Orschell, Christie M / Chan, Edward M / Yu, Zhangsheng / Hood, Daniel / Plett, Artur / Yang, Zhenyun / Hui, Chua Lin / Nabinger, Sarah C / Kohlbacher, Kristopher J / West, Evan S / Walter, Amanda / Sampson, Carol / Wu, Jingwei / Cripe, Larry D

    Leukemia research

    2011  Volume 35, Issue 8, Page(s) 1108–1110

    Abstract: Prolonged administration of methyl transferase inhibitors may increase response rates in myelodysplastic syndromes (MDS). Fourteen MDS patients with anemia and less than 10% marrow blasts received azacitidine 50 mg/m(2) thrice weekly for 2 weeks every 4 ... ...

    Abstract Prolonged administration of methyl transferase inhibitors may increase response rates in myelodysplastic syndromes (MDS). Fourteen MDS patients with anemia and less than 10% marrow blasts received azacitidine 50 mg/m(2) thrice weekly for 2 weeks every 4 weeks; 7 also received weekly erythropoietin. The response rate of 43% did not improve the rates reported with other azacitidine administration schedules, so the study was closed. A decreased apoptosis of primitive erythroid progenitors and increased expression of BclX(L) was observed with treatment in responding patients compared to non-responders. Azacitidine may modulate BclX(L) and improve erythropoiesis through reduction of apoptosis in primitive erythroid progenitor population in MDS.
    MeSH term(s) Aged ; Aged, 80 and over ; Anemia/drug therapy ; Anemia/etiology ; Antimetabolites, Antineoplastic/therapeutic use ; Apoptosis/drug effects ; Azacitidine/therapeutic use ; Erythroid Precursor Cells/drug effects ; Erythropoiesis/drug effects ; Erythropoietin/therapeutic use ; Female ; Flow Cytometry ; Humans ; Male ; Middle Aged ; Myelodysplastic Syndromes/complications ; Myelodysplastic Syndromes/therapy ; Prognosis ; bcl-X Protein/metabolism
    Chemical Substances Antimetabolites, Antineoplastic ; BCL2L1 protein, human ; bcl-X Protein ; Erythropoietin (11096-26-7) ; Azacitidine (M801H13NRU)
    Language English
    Publishing date 2011-08
    Publishing country England
    Document type Journal Article ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
    ZDB-ID 752396-8
    ISSN 1873-5835 ; 0145-2126
    ISSN (online) 1873-5835
    ISSN 0145-2126
    DOI 10.1016/j.leukres.2011.02.025
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1321: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  4. Article: Shp-2 heterozygous hematopoietic stem cells have deficient repopulating ability due to diminished self-renewal.

    Chan, Rebecca J / Li, Yanjun / Hass, Meredith N / Walter, Amanda / Voorhorst, Cara S / Shelley, W Chris / Yang, Zhenyun / Orschell, Christie M / Yoder, Mervin C

    Experimental hematology

    2006  Volume 34, Issue 9, Page(s) 1230–1239

    Abstract: Objective: Improved understanding of hematopoietic stem cell (HSC) differentiation, proliferation, and self-renewal is sought to develop improved stem cell-based therapies as well as to define novel therapies for stem cell-based diseases such as ... ...

    Abstract Objective: Improved understanding of hematopoietic stem cell (HSC) differentiation, proliferation, and self-renewal is sought to develop improved stem cell-based therapies as well as to define novel therapies for stem cell-based diseases such as leukemia. Shp-2 is a widely expressed nonreceptor protein tyrosine phosphatase that participates early in hematopoietic development. The following study was performed to examine the role of Shp-2 in HSC function.
    Methods: Bone marrow low-density mononuclear cells were isolated from WT and Shp-2(+/-) littermate controls and utilized in competitive repopulation studies, homing analysis, cell-cycle analysis, and serial transplantation studies.
    Results: Haploinsufficiency of Shp-2 causes a threefold reduction in HSC repopulating units following transplantation into lethally irradiated recipients. Homing of Shp-2(+/-) and WT cells to the bone marrow and spleen compartments was equal. Cell-cycle analysis studies revealed that the Shp-2(+/-) lin(-)Sca-1(+)c-kit(+) cells are less quiescent than WT cells, providing a potential etiology for the observed reduced engraftment of the Shp-2(+/-) cells. Consistently, in serial transplantation studies, we observed a significant reduction of Shp-2(+/-) self-renewal compared to that of WT cells.
    Conclusion: These data demonstrate that Shp-2 is required for the physiologic homeostasis of the HSC compartment and potentially provide insight into how oncogenic Shp-2 may contribute to the pathogenesis of myeloproliferative disorders and leukemias.
    MeSH term(s) Animals ; Antigens, Ly/genetics ; Antigens, Ly/metabolism ; Bone Marrow/metabolism ; Bone Marrow/pathology ; Cell Cycle/genetics ; Cell Movement/genetics ; Graft Survival/genetics ; Hematopoietic Stem Cell Transplantation/methods ; Hematopoietic Stem Cells/metabolism ; Hematopoietic Stem Cells/pathology ; Heterozygote ; Intracellular Signaling Peptides and Proteins/genetics ; Intracellular Signaling Peptides and Proteins/metabolism ; Leukemia/genetics ; Leukemia/metabolism ; Leukemia/pathology ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Mice ; Mice, Transgenic ; Protein Tyrosine Phosphatase, Non-Receptor Type 11 ; Protein Tyrosine Phosphatases/genetics ; Protein Tyrosine Phosphatases/metabolism ; Proto-Oncogene Proteins c-kit/genetics ; Proto-Oncogene Proteins c-kit/metabolism ; Spleen/metabolism ; Spleen/pathology
    Chemical Substances Antigens, Ly ; Intracellular Signaling Peptides and Proteins ; Ly6a protein, mouse ; Membrane Proteins ; Proto-Oncogene Proteins c-kit (EC 2.7.10.1) ; Protein Tyrosine Phosphatase, Non-Receptor Type 11 (EC 3.1.3.48) ; Protein Tyrosine Phosphatases (EC 3.1.3.48) ; Ptpn11 protein, mouse (EC 3.1.3.48)
    Language English
    Publishing date 2006-09
    Publishing country Netherlands
    Document type Comparative Study ; Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 185107-x
    ISSN 1873-2399 ; 0301-472X ; 0531-5573
    ISSN (online) 1873-2399
    ISSN 0301-472X ; 0531-5573
    DOI 10.1016/j.exphem.2006.04.017
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 922: Show issues Location:
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