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  1. Article ; Online: Enterohemorrhagic

    Fang, Yuting / Fu, Muqing / Li, Xinyue / Zhang, Bao / Wan, Chengsong

    Infection and immunity

    2024  Volume 92, Issue 4, Page(s) e0000124

    Abstract: Attaching/effacing (A/E) pathogens induce DNA damage and colorectal cancer by injecting effector proteins into host cells via the type III secretion system (T3SS). EspF is one of the T3SS-dependent effector proteins exclusive to A/E pathogens, which ... ...

    Abstract Attaching/effacing (A/E) pathogens induce DNA damage and colorectal cancer by injecting effector proteins into host cells via the type III secretion system (T3SS). EspF is one of the T3SS-dependent effector proteins exclusive to A/E pathogens, which include enterohemorrhagic
    MeSH term(s) Humans ; Enterohemorrhagic Escherichia coli ; Epithelial Cells ; DNA Repair ; DNA Damage ; Oxidative Stress ; Colorectal Neoplasms
    Language English
    Publishing date 2024-02-28
    Publishing country United States
    Document type Journal Article
    ZDB-ID 218698-6
    ISSN 1098-5522 ; 0019-9567
    ISSN (online) 1098-5522
    ISSN 0019-9567
    DOI 10.1128/iai.00001-24
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 684: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Effects of a Shift of the Signal Peptide Cleavage Site in Signal Peptide Variant on the Synthesis and Secretion of SARS-CoV-2 Spike Protein.

    Zhang, Zhikai / Wan, Xuan / Li, Xinyue / Wan, Chengsong

    Molecules (Basel, Switzerland)

    2022  Volume 27, Issue 19

    Abstract: The COVID-19 pandemic is caused by SARS-CoV-2; the spike protein is a key structural protein that mediates infection of the host by SARS-CoV-2. In this study, we aimed to evaluate the effects of signal peptide on the secretion and release of SARS-CoV-2 ... ...

    Abstract The COVID-19 pandemic is caused by SARS-CoV-2; the spike protein is a key structural protein that mediates infection of the host by SARS-CoV-2. In this study, we aimed to evaluate the effects of signal peptide on the secretion and release of SARS-CoV-2 spike protein. Therefore, we constructed a signal peptide deletion mutant and three signal peptide site-directed mutants. The (H) region and (C) region in the signal peptide of L5F-S13I mutant have changed significantly, compared with wild type, L5F and S13I. We demonstrated the effects of signal peptide on the secretion and synthesis of RBD protein, finding that mutation of S13 to I13 on the signal peptide is more conducive to the secretion of RBD protein, which was mainly due to the shift of the signal peptide cleavage site in the mutant S13I. Here, we not only investigated the structure of the N-terminal signal peptide of the SARS-CoV-2 spike protein but also considered possible secretory pathways. We suggest that the development of drugs that target the signal peptide of the SARS-CoV-2 spike protein may have potential to treat COVID-19 in the future.
    MeSH term(s) COVID-19 ; Humans ; Pandemics ; Protein Sorting Signals/genetics ; SARS-CoV-2/genetics ; Spike Glycoprotein, Coronavirus/metabolism
    Chemical Substances Protein Sorting Signals ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
    Language English
    Publishing date 2022-10-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules27196688
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.MO 81: Show issues

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  3. Article ; Online: Enhancing the Immunogenicity of RBD Protein Variants through Amino Acid E484 Mutation in SARS-CoV-2.

    Zhang, Zhikai / Wan, Xuan / Li, Xinyue / Cai, Shaoxi / Wan, Chengsong

    Viruses

    2022  Volume 14, Issue 9

    Abstract: In the context of the COVID-19 pandemic, conducting antibody testing and vaccination is critical. In particular, the continued evolution of SARS-CoV-2 raises concerns about the effectiveness of vaccines currently in use and the activity of neutralizing ... ...

    Abstract In the context of the COVID-19 pandemic, conducting antibody testing and vaccination is critical. In particular, the continued evolution of SARS-CoV-2 raises concerns about the effectiveness of vaccines currently in use and the activity of neutralizing antibodies. Here, we used the
    MeSH term(s) Amino Acids/genetics ; Animals ; Antibodies, Neutralizing/chemistry ; Antibodies, Viral ; COVID-19/prevention & control ; Escherichia coli/genetics ; Humans ; Mice ; Mutant Proteins/genetics ; Mutation ; Neutralization Tests ; Pandemics ; Recombinant Proteins ; SARS-CoV-2/genetics ; Spike Glycoprotein, Coronavirus ; Viral Vaccines
    Chemical Substances Amino Acids ; Antibodies, Neutralizing ; Antibodies, Viral ; Mutant Proteins ; Recombinant Proteins ; Spike Glycoprotein, Coronavirus ; Viral Vaccines ; spike protein, SARS-CoV-2
    Language English
    Publishing date 2022-09-13
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14092020
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Calpain-2 protein influences

    Li, Jia / Zheng, Kang / Shen, Huilong / Wu, Hua / Wan, Chengsong / Zhang, Renli / Liu, Zhimin

    Frontiers in microbiology

    2023  Volume 14, Page(s) 1229576

    Abstract: Chikungunya fever (CHIF), a vector-borne disease transmitted mainly ... ...

    Abstract Chikungunya fever (CHIF), a vector-borne disease transmitted mainly by
    Language English
    Publishing date 2023-10-19
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2023.1229576
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Enhancing the Immunogenicity of RBD Protein Variants through Amino Acid E484 Mutation in SARS-CoV-2

    Zhang, Zhikai / Wan, Xuan / Li, Xinyue / Cai, Shaoxi / Wan, Chengsong

    Viruses. 2022 Sept. 13, v. 14, no. 9

    2022  

    Abstract: In the context of the COVID-19 pandemic, conducting antibody testing and vaccination is critical. In particular, the continued evolution of SARS-CoV-2 raises concerns about the effectiveness of vaccines currently in use and the activity of neutralizing ... ...

    Abstract In the context of the COVID-19 pandemic, conducting antibody testing and vaccination is critical. In particular, the continued evolution of SARS-CoV-2 raises concerns about the effectiveness of vaccines currently in use and the activity of neutralizing antibodies. Here, we used the Escherichia coli expression system to obtain nine different SARS-CoV-2 RBD protein variants, including six single-point mutants, one double-point mutant, and two three-point mutants. Western blotting results show that nine mutants of the RBD protein had strong antigenic activity in vitro. The immunogenicity of all RBD proteins was detected in mice to screen for protein mutants with high immunogenicity. The results show that the mutants E484K, E484Q, K417T-E484K-N501Y, and K417N-E484K-N501Y, especially the former two, had better immunogenicity than the wild type. This suggests that site E484 has a significant impact on the function of the RBD protein. Our results demonstrate that recombinant RBD protein expressed in E. coli can be an effective tool for the development of antibody detection methods and vaccines.
    Keywords COVID-19 infection ; Escherichia coli ; Severe acute respiratory syndrome coronavirus 2 ; amino acids ; antibodies ; antibody detection ; evolution ; immunogenicity ; mutants ; mutation ; vaccination
    Language English
    Dates of publication 2022-0913
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14092020
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: [A fluorometric method for direct detection of inorganic polyphosphate in enterohemorrhagic

    Du, Yanli / Han, Zongli / Wang, Xiangyu / Wan, Chengsong

    Nan fang yi ke da xue xue bao = Journal of Southern Medical University

    2019  Volume 39, Issue 3, Page(s) 344–350

    Abstract: Objective: To establish a quantitative fluorescent detection method using DAPI for detecting inorganic polyphosphate (polyP) in enterohemorrhagic Escherichia coli (EHEC) O157:H7.: Methods: The DNA of wild-type strain of EHEC O157:H7 was extracted and ...

    Abstract Objective: To establish a quantitative fluorescent detection method using DAPI for detecting inorganic polyphosphate (polyP) in enterohemorrhagic Escherichia coli (EHEC) O157:H7.
    Methods: The DNA of wild-type strain of EHEC O157:H7 was extracted and purified. DAPI was combined with the extracted DNA and polyP45 standards for measurement of the emission spectra at 360 nm and 415 nm fluorescence spectrophotometry. The fluorescence of DAPI-DNA and DAPI-polyP complexes was detected by fluorescence confocal microscopy to verify the feasibility of DAPI for detecting polyP. To determine the optimal pretreatment protocol for improving the cell membrane permeability, the effects of 6 pretreatments of the cells (namely snap-freezing in liquid nitrogen, freezing at -80 ℃, and freezing at -20 ℃, all followed by thawing at room temperature; heating at 60 ℃ for 10 min; treatment with Triton x-100; and placement at room temperature) were tested on the survival of EHEC O157:H7. The fluorescence values of the treated bacteria were then measured after DAPI staining. A standard calibration curve of polyP standard was established for calculation of the content of polyP in the live cells of wildtype EHEC strain and two
    Results: At the excitation wavelength of 360 nm, the maximum emission wavelength of DAPI-DNA was 460 nm, and the maximum emission wavelength of DAPI-polyP was 550 nm at the excitation wavelength of 415 nm. The results of confocal microscopy showed that 405 nm excitation elicited blue fluorescence from DAPIDNA complex with the emission wavelength of 425-475 nm; excitation at 488 nm elicited green fluorescence from the DAPIpolyP complex with the emission wavelength of 500-560 nm of. Snap-freezing of cells at -80 ℃ followed by thawing at room temperature was the optimal pretreatment to promote DAPI penetration into the live cells. The standard calibration curve was
    Conclusions: We established a convenient quantitative method for direct and reliable detection polyP content to facilitate further study of polyP and its catalytic enzymes in EHEC O157:H7.
    MeSH term(s) Escherichia coli O157 ; Escherichia coli Proteins ; Polyphosphates
    Chemical Substances Escherichia coli Proteins ; Polyphosphates
    Language Chinese
    Publishing date 2019-05-08
    Publishing country China
    Document type Journal Article
    ZDB-ID 2250951-3
    ISSN 1673-4254
    ISSN 1673-4254
    DOI 10.12122/j.issn.1673-4254.2019.03.13
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6344: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  7. Article ; Online: Development of monoclonal antibodies targeting the conserved fragment of hexon protein to detect different serotypes of human adenovirus.

    Wu, Linfan / Lin, Yuhao / Yin, Juzhen / Yang, Changbi / Jiang, Yushan / Zhai, Linlin / Wang, Yuelin / Zhu, Li / Wu, Qinghua / Zhang, Bao / Wan, Chengsong / Zhao, Wei / Yang, Yang / Shen, Chenguang / Xiao, Weiwei

    Microbiology spectrum

    2024  Volume 12, Issue 4, Page(s) e0181623

    Abstract: Human adenovirus (HAdV) infects the respiratory system, thus posing a threat to health. However, immunodiagnostic reagents for human adenovirus are limited. This study aimed to develop efficient diagnostic reagents based on monoclonal antibodies for ... ...

    Abstract Human adenovirus (HAdV) infects the respiratory system, thus posing a threat to health. However, immunodiagnostic reagents for human adenovirus are limited. This study aimed to develop efficient diagnostic reagents based on monoclonal antibodies for diagnosing various human adenovirus infections. Evolutionary and homology analyses of various human adenoviral antigen genes revealed highly conserved antigenic fragments. The prokaryotic expression system was applied to recombinant penton, hexon, and IVa2 conserved fragments of adenovirus, which were injected into BALB/c mice to prepare human adenovirus-specific monoclonal antibodies. Enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence assay (IFA), and Western blotting were used to determine the immune specificity of the monoclonal antibodies. Indirect ELISA showed that monoclonal antibodies 1F10, 8D3, 4A1, and 9B2 were specifically bound to HAdV-3 and HAdV-55 and revealed high sensitivity and low detection limits for various human adenoviruses. Western blotting showed that 1F10 and 8D3 specifically recognized various human adenovirus types, including HAdV-1, HAdV-2, HAdV-3, HAdV-4, HAdV-5, HAdV-7, HAdV-21, and HAdV-55, and 4A1 specifically recognized HAdV-1, HAdV-2, HAdV-3, HAdV-5, HAdV-7, HAdV-21, and HAdV-55. IFAs showed that 1F10, 8D3, and 4A1 exhibited highly selective localization to A549 cells infected with HAdV-3 and HAdV-55. Finally, two antibody pairs that could detect hexon antigens HAdV-3 and HAdV-55 at low concentrations were developed. The monoclonal antibodies developed in this study show potential for detecting human adenoviruses.
    Importance: In this study, we selected the three most conserved antigenic fragments of human adenovirus to prepare a murine monoclonal antibody for the first time, and human adenovirus antigenic fragments with heretofore unheard of degrees of conservatism were isolated. The three monoclonal antibodies with the ability to recognize human respiratory adenovirus over a broad spectrum were screened by hybridoma and monoclonal antibody preparation. Human adenovirus infections are serious; however, therapeutic drugs and diagnostic reagents are scarce. Thus, to reduce the serious consequences of human viral infections and adenovirus pneumonitis, early diagnosis of infection is required. The present study provides three monoclonal antibodies capable of recognizing a wide range of human adenoviruses, thereby offering guidance for subsequent research and development.
    MeSH term(s) Humans ; Animals ; Mice ; Antibodies, Monoclonal ; Antibodies, Viral ; Adenoviruses, Human/genetics ; Adenovirus Infections, Human ; Serogroup ; Capsid Proteins/genetics
    Chemical Substances Antibodies, Monoclonal ; Antibodies, Viral ; Capsid Proteins
    Language English
    Publishing date 2024-02-22
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.01816-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Clever Cooperation: Interactions Between EspF and Host Proteins.

    Hua, Ying / Yan, Kaina / Wan, Chengsong

    Frontiers in microbiology

    2018  Volume 9, Page(s) 2831

    Abstract: EspF is a central effector protein of ... ...

    Abstract EspF is a central effector protein of enterohemorrhagic
    Language English
    Publishing date 2018-11-22
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2018.02831
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Erratum for Li et al., "Luciferase Immunosorbent Assay Based on Multiple E Antigens for the Detection of Chikungunya Virus-Specific IgG Antibodies".

    Li, Xiaoxia / Wan, Xuan / Liu, Jinyue / Wang, Haiying / Li, Anan / Ke, Changwen / Tang, Shixing / Zhao, Wei / Cai, Shaoxi / Wan, Chengsong

    Microbiology spectrum

    2022  Volume 10, Issue 4, Page(s) e0228222

    Language English
    Publishing date 2022-07-05
    Publishing country United States
    Document type Journal Article ; Published Erratum
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.02282-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: EspF of Enterohemorrhagic

    Wang, Xiangyu / Yan, Kaina / Fu, Muqing / Liang, Song / Zhao, Haiyi / Fu, Changzhu / Yang, Lan / Song, Zhihong / Sun, Dayong / Wan, Chengsong

    Frontiers in microbiology

    2022  Volume 13, Page(s) 900919

    Abstract: There have been large foodborne outbreaks related to ... ...

    Abstract There have been large foodborne outbreaks related to Enterohemorrhagic
    Language English
    Publishing date 2022-06-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2022.900919
    Database MEDical Literature Analysis and Retrieval System OnLINE

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