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Article ; Online: miR-613 inhibits the proliferation of human ovarian granulosa cells by arresting cell cycle progression via the targeting of IGF-1.

Wan, Junhui / Liu, Sisun

2021 Volume 23, Issue 3

Abstract: Polycystic ovary syndrome (PCOS) is a prevalent endocrine disorder, and microRNA (miRNA) molecules have been implicated in the pathological process of PCOS. The aim of the present study was to elucidate the regulatory effects of miR-613 and insulin-like ... ...

Abstract	Polycystic ovary syndrome (PCOS) is a prevalent endocrine disorder, and microRNA (miRNA) molecules have been implicated in the pathological process of PCOS. The aim of the present study was to elucidate the regulatory effects of miR-613 and insulin-like growth factor-1 (IGF-1) on the pathological process of polycystic ovary syndrome (PCOS). The targeting of IGF-1 by miR-613 was investigated by dual-luciferase reporter assay. The regulatory effect of miR-613 on the mRNA and protein levels of IGF1 was determined by reverse transcription-quantitative PCR and western blot analysis. The regulatory effects of miR-613 and IGF-1 on the proliferation and cell cycle progression of KGN cells were evaluated by colony formation assay and flow cytometric analysis. The results revealed that miR-613 targeted IGF-1 and reduced its translational level. In KGN cells, miR-613 arrested cell cycle progression in the G2/M phase and downregulated the expression of cyclin D1 and CDK1. The overexpression of IGF-1 attenuated the inhibitory effects of miR-613 on cell cycle arrest, cyclin D1 and CDK1 expression, and the proliferation of KGN cells. In conclusion, the present study demonstrated that miR-613 targets IGF-1 and thus suppresses its translation. It arrests cell cycle progression and attenuates the proliferation of KGN cells via the targeting of IGF-1. Therefore, it is suggested that miR-613 and IGF-1 could potentially be diagnostic biomarkers and therapeutic targets for PCOS.
MeSH term(s)	Cell Cycle Checkpoints ; Female ; Granulosa Cells/cytology ; Granulosa Cells/metabolism ; Humans ; Insulin-Like Growth Factor I/metabolism ; MicroRNAs/metabolism
Chemical Substances	IGF1 protein, human ; MIRN613 microRNA, human ; MicroRNAs ; Insulin-Like Growth Factor I (67763-96-6)
Language	English
Publishing date	2021-01-05
Publishing country	Greece
Document type	Journal Article
ZDB-ID	2469505-1
ISSN	1791-3004 ; 1791-2997
ISSN (online)	1791-3004
ISSN	1791-2997
DOI	10.3892/mmr.2020.11817
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Rosiglitazone ameliorates palmitic acid-induced endoplasmic reticulum stress and steroidogenic capacity in granulosa cells.

Wan, Junhui / Liu, Sisun

Reproductive biology

2020 Volume 20, Issue 3, Page(s) 293–299

Abstract: Granulosa cells play essential roles in follicular development, oocyte maturation and sex hormone secretion. The exposure of granulosa cells to palmitic acid (PA), the main component of dietary saturated fat, inhibits cell viability. However, the ... ...

Abstract	Granulosa cells play essential roles in follicular development, oocyte maturation and sex hormone secretion. The exposure of granulosa cells to palmitic acid (PA), the main component of dietary saturated fat, inhibits cell viability. However, the mechanism underlying PA-induced cytotoxicity in granulosa cells has not been deeply investigated. Rosiglitazone (RSG) is a member of the thiazolidinedione family and is reported to protect cells from cytotoxicity and endoplasmic reticulum (ER) stress in other cell types, but whether RSG protects granulosa cells remain unknown. In this study, KGN cell line and primary granulosa cells were used as models of granulosa cells to explore the effects of PA and RSG and the underlying mechanisms. The results showed that PA inhibits cell viability and estradiol secretion through inducing ER stress and cAMP/PKA/CREB pathway. CCAAT/enhancer-binding protein homologous protein (CHOP), an ER stress marker, was demonstrated to participate in PA-induced cytotoxicity. RSG treatment rescued granulosa cells from PA-induced cell death and ER stress. Moreover, RSG was identified to ameliorate ER stress induced by tunicamycin in granulosa cells. In addition, RSG treatment rescued granulosa cells from PA-induced decrease of estrogen secretion by cAMP/PKA/CREB pathway. In conclusion, RSG can protect granulosa cells against PA-induced cytotoxicity by inhibiting ER stress, and can recover steroidogenic capacity, indicating a potential use of RSG in the treatment of granulosa cell dysfunction.
Language	English
Publishing date	2020-07-28
Publishing country	Poland
Document type	Journal Article
ZDB-ID	2189316-0
ISSN	2300-732X ; 1642-431X
ISSN (online)	2300-732X
ISSN	1642-431X
DOI	10.1016/j.repbio.2020.07.004
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Article: Rosiglitazone ameliorates palmitic acid-induced endoplasmic reticulum stress and steroidogenic capacity in granulosa cells

Wan, Junhui / Liu, Sisun

Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn Reproductive Biology. 2020 Sept., v. 20, no. 3

2020

Abstract	Granulosa cells play essential roles in follicular development, oocyte maturation and sex hormone secretion. The exposure of granulosa cells to palmitic acid (PA), the main component of dietary saturated fat, inhibits cell viability. However, the mechanism underlying PA-induced cytotoxicity in granulosa cells has not been deeply investigated. Rosiglitazone (RSG) is a member of the thiazolidinedione family and is reported to protect cells from cytotoxicity and endoplasmic reticulum (ER) stress in other cell types, but whether RSG protects granulosa cells remain unknown. In this study, KGN cell line and primary granulosa cells were used as models of granulosa cells to explore the effects of PA and RSG and the underlying mechanisms. The results showed that PA inhibits cell viability and estradiol secretion through inducing ER stress and cAMP/PKA/CREB pathway. CCAAT/enhancer-binding protein homologous protein (CHOP), an ER stress marker, was demonstrated to participate in PA-induced cytotoxicity. RSG treatment rescued granulosa cells from PA-induced cell death and ER stress. Moreover, RSG was identified to ameliorate ER stress induced by tunicamycin in granulosa cells. In addition, RSG treatment rescued granulosa cells from PA-induced decrease of estrogen secretion by cAMP/PKA/CREB pathway. In conclusion, RSG can protect granulosa cells against PA-induced cytotoxicity by inhibiting ER stress, and can recover steroidogenic capacity, indicating a potential use of RSG in the treatment of granulosa cell dysfunction.
Keywords	cAMP-dependent protein kinase ; cell death ; cell viability ; cyclic AMP ; cytotoxicity ; endoplasmic reticulum stress ; estradiol ; follicular development ; granulosa cells ; hormone secretion ; models ; oocytes ; palmitic acid ; saturated fats ; tunicamycin
Language	English
Dates of publication	2020-09
Size	p. 293-299.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	2189316-0
ISSN	1642-431X
ISSN	1642-431X
DOI	10.1016/j.repbio.2020.07.004
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Article: Long Non-Coding RNA HULC Promotes Cervical Cancer Cell Proliferation, Migration and Invasion via miR-218/TPD52 Axis.

Lu, Wenjun / Wan, Xiaobin / Tao, Limin / Wan, Junhui

OncoTargets and therapy

2020 Volume 13, Page(s) 1109–1118

Abstract: Objective: Long non-coding RNAs (lncRNAs) have been identified as important players in tumorigenesis. LncRNA highly upregulated in liver cancer (HULC) has been identified as a key regulator in the progression of various cancers. However, the functional ... ...

Abstract	Objective: Long non-coding RNAs (lncRNAs) have been identified as important players in tumorigenesis. LncRNA highly upregulated in liver cancer (HULC) has been identified as a key regulator in the progression of various cancers. However, the functional role and the mechanisms of HULC in regulating cervical cancer cell behavior remain unclear. Methods: HULC expression, miR-218 expression and TPD52 mRNA level in cervical cancer cells were examined by qRT-PCR. Cell proliferation was evaluated by MTT assay. Cell migration and invasion were examined by Transwell assay. TPD52 protein level was measured by Western blot. Dual-luciferase reporter assay was measured to verify the combination of HULC and miR-218 as well as miR-218 and TPD52. Results: HULC expression was upregulated in cervical cancer cell lines, and HULC promoted cervical cancer cell proliferation, migration and invasion. Mechanistically, HULC acted as a sponge of miR-218 to elevate expression of TPD52, a target of miR-218, and thereby promoted cervical cancer cell proliferation, migration, and invasion. Conclusion: HULC promotes cervical cancer cell proliferation, migration and invasion via miR-218/TPD52 axis.
Language	English
Publishing date	2020-02-05
Publishing country	New Zealand
Document type	Journal Article
ZDB-ID	2495130-4
ISSN	1178-6930
ISSN	1178-6930
DOI	10.2147/OTT.S232914
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Article: Prenatal Silver-Russell Syndrome in a Chinese Family Identified by Non-Invasive Prenatal Testing.

Zhang, Yong-Ling / Jing, Xiang-Yi / Wan, Jun-Hui / Pan, Min / Li, Dong-Zhi

Molecular syndromology

2022 Volume 13, Issue 4, Page(s) 323–327

Abstract: Russell-Silver syndrome (SRS) is a rare condition characterized by poor growth before and after birth along with multiple physical and psychosocial characteristics such as short stature, characteristic facial features, body asymmetry, feeding ... ...

Abstract	Russell-Silver syndrome (SRS) is a rare condition characterized by poor growth before and after birth along with multiple physical and psychosocial characteristics such as short stature, characteristic facial features, body asymmetry, feeding difficulties, and learning disabilities. In this study, we report a family with 2 recurrent SRS pregnancies due to a derivative chromosome 15 that is the result of a maternally derived t(11;15) translocation, detected by non-invasive prenatal testing (NIPT). The 2 SRS fetuses were diagnosed by chromosomal microarray analysis, but a balanced, reciprocal translocation of the mother was disclosed by the combination of routine karyotyping and FISH. This study demonstrates that NIPT has the ability to identify submicroscopic copy number variations (CNVs) in fetuses, which in some cases may result from a parent being a balanced rearrangement carrier. Because of the differences in resolution and the various benefits and limitations of each genetic technique, great care must be taken when deciding on which test(s) to employ in family studies.
Language	English
Publishing date	2022-02-04
Publishing country	Switzerland
Document type	Case Reports
ZDB-ID	2546218-0
ISSN	1661-8777 ; 1661-8769
ISSN (online)	1661-8777
ISSN	1661-8769
DOI	10.1159/000520389
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Article ; Online: Research Progress of HIV-1 Nef Inhibitors.

Lv, Yanhan / Meng, Yuanxi / Zhong, Jiaqian / Wan, Junhui / Zou, Wei

AIDS reviews

2020 Volume 22, Issue 4, Page(s) 221–226

Abstract: HIV-1 infection poses a major threat to the public health worldwide. The antiretroviral agents that are currently used to treat HIV-1 infection target viral reverse transcriptase, integrase and protease, or block the fusion of viral envelop and cell ... ...

Abstract	HIV-1 infection poses a major threat to the public health worldwide. The antiretroviral agents that are currently used to treat HIV-1 infection target viral reverse transcriptase, integrase and protease, or block the fusion of viral envelop and cell membrane. Studies have shown that the HIV-1 encoded protein Nef plays an important role in the pathogenesis of viral infection. Nef ensures efficient counterattack against host immune responses as well as long-term evasion of immune surveillance. In addition, Nef, expressing at a high level early in the viral life cycle, is required for maintaining a high viral load in the persistent infection in vivo and for full pathologic potential. Therefore, Nef may be an excellent target to treat HIV-1 infection. In this manuscript, we reviewed five potential Nef inhibitors, namely, DLC27-14, t ightly bound hydroxypyrazole HIV-1 Nef inhibitor B9, 2c-like inhibitors, N-(3-aminoquinoxalin-2-yl)-4-chlorobenzenesulfonamide and compound 1[(7-oxo-7H-benzo[anthracene]-3-yl)amino]anthraquinone, and their working mechanisms. These drugs may be further developed into new regimens for the treatment of HIV-1 infection.
MeSH term(s)	Anti-HIV Agents/pharmacology ; Gene Expression Regulation, Viral/drug effects ; HIV Infections/drug therapy ; HIV-1/drug effects ; Humans ; nef Gene Products, Human Immunodeficiency Virus/antagonists & inhibitors
Chemical Substances	Anti-HIV Agents ; nef Gene Products, Human Immunodeficiency Virus
Language	English
Publishing date	2020-10-26
Publishing country	Spain
Document type	Journal Article ; Review
ZDB-ID	2086783-9
ISSN	1698-6997 ; 1139-6121
ISSN (online)	1698-6997
ISSN	1139-6121
DOI	10.24875/AIDSRev.20000050
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Zs.A 5711: Show issues

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Article: Tumor-Infiltrating CD8+ T Cells Driven by the Immune Checkpoint-Associated Gene IDO1 Are Associated With Cervical Cancer Prognosis.

Zhang, Shun / Wan, Junhui / Chen, Minjie / Cai, Desheng / Xu, Junlan / Chen, Qi

Frontiers in oncology

2021 Volume 11, Page(s) 720447

Abstract: Tumor-infiltrating immune cells, associated with tumor progression, are promising prognostic biomarkers. However, the relationship between levels of gene expression and that of immune cell infiltration in cervical cancer prognosis is unknown. In this ... ...

Abstract	Tumor-infiltrating immune cells, associated with tumor progression, are promising prognostic biomarkers. However, the relationship between levels of gene expression and that of immune cell infiltration in cervical cancer prognosis is unknown. In this study, three cervical cancer gene expression microarrays (GSE6791, GSE63678 and GSE55940) were obtained from the GEO database. The IDO1 gene was identified by differentially expressed gene screening. The gene expression profiles of TCGA and GTEx databases along with comprehensive bioinformatics analysis identified that the IDO1 gene was upregulated in cervical cancer with significant difference in expression at different N stages. In addition, it was also upregulated in HPV16 positive sample. The pan-cancer analysis identified that IDO1 was highly expressed in most cancers. TIMER analysis revealed that the expression of IDO1 in CESC shows positive correlation with CD8
Language	English
Publishing date	2021-10-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2649216-7
ISSN	2234-943X
ISSN	2234-943X
DOI	10.3389/fonc.2021.720447
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Article ; Online: Uterine necrosis, infection, and subinvolution: complications observed after combined application of modified B-Lynch suture and vascular ligation.

Luo, Linfeng / Wan, Junhui / Chen, Xinping / Zhang, Huan / Zhang, Minjie / Chen, Qi

The Journal of international medical research

2021 Volume 49, Issue 5, Page(s) 3000605211010730

Abstract: Compression sutures are primarily used to treat atonic postpartum hemorrhage. We herein describe three cases of selective arterial ligation combined with B-Lynch or modified B-Lynch suture for the treatment of intractable postpartum hemorrhage ... ...

Abstract	Compression sutures are primarily used to treat atonic postpartum hemorrhage. We herein describe three cases of selective arterial ligation combined with B-Lynch or modified B-Lynch suture for the treatment of intractable postpartum hemorrhage unresponsive to available conservative interventions. Three pregnant women underwent a cesarean section for a macrosomic fetus, fetal distress, and oligohydramnios, respectively. All three women developed intractable postpartum hemorrhage due to uterine atony with no chance of embolization therapy. B-Lynch or modified B-Lynch suture and additional selective arterial ligation were performed using braided absorbable suture. The first woman developed postoperative hematometra and infection without response to drainage and antibiotic therapy. Although laparoscopic exploration was performed to loosen the suture line and drain the hematometra and pyometra, the necrosis and infection could not be controlled. Subtotal hysterectomy was therefore conducted, and the necrotic uterine adnexa was removed. The other two women developed subinvolution of the uterus resulting in prolonged menstruation and amenorrhea, although the uterus was preserved and the bleeding was controlled. Modified B-Lynch suture combined with vascular ligation is an invaluable technique for women with severe intractable postpartum hemorrhage. However, it can lead to serious complications such as uterine necrosis, infection, and subinvolution.
MeSH term(s)	Cesarean Section/adverse effects ; Female ; Humans ; Ligation/adverse effects ; Necrosis ; Postpartum Hemorrhage/etiology ; Postpartum Hemorrhage/surgery ; Pregnancy ; Retrospective Studies ; Suture Techniques ; Sutures/adverse effects ; Uterus/diagnostic imaging ; Uterus/surgery
Language	English
Publishing date	2021-05-05
Publishing country	England
Document type	Journal Article
ZDB-ID	184023-x
ISSN	1473-2300 ; 0300-0605 ; 0142-2596
ISSN (online)	1473-2300
ISSN	0300-0605 ; 0142-2596
DOI	10.1177/03000605211010730
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Zs.A 925: Show issues

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Article ; Online: Insufficient fetal fraction of cell-free DNA in non-invasive prenatal testing: Not always true.

Wan, Jun-Hui / Li, Fa-Tao / Li, Ru / Zhong, Hui-Zhu / Li, Dong-Zhi

European journal of obstetrics, gynecology, and reproductive biology

2021 Volume 261, Page(s) 245–247

MeSH term(s)	Aneuploidy ; Cell-Free Nucleic Acids ; Female ; Fetus ; Genetic Testing ; Humans ; Pregnancy ; Prenatal Diagnosis
Chemical Substances	Cell-Free Nucleic Acids
Language	English
Publishing date	2021-04-21
Publishing country	Ireland
Document type	Letter
ZDB-ID	190605-7
ISSN	1872-7654 ; 0301-2115 ; 0028-2243
ISSN (online)	1872-7654
ISSN	0301-2115 ; 0028-2243
DOI	10.1016/j.ejogrb.2021.04.026
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Article ; Online: Confined placental trisomy detection through non-invasive prenatal testing: benefit for pregnancy management.

Wan, Jun-Hui / He, Ping / Xu, Li-Li / Li, Dong-Zhi

Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology

2019 Volume 40, Issue 7, Page(s) 1020–1022

MeSH term(s)	Adult ; Amniocentesis ; Cesarean Section ; Chromosomes, Human, Pair 16 ; Female ; Gestational Age ; Humans ; Infant, Newborn ; Karyotyping ; Mosaicism ; Placenta ; Pregnancy ; Pregnancy Outcome ; Prenatal Diagnosis/methods ; Trisomy/diagnosis ; Uniparental Disomy/genetics
Language	English
Publishing date	2019-11-26
Publishing country	England
Document type	Case Reports ; Journal Article
ZDB-ID	604639-3
ISSN	1364-6893 ; 0144-3615
ISSN (online)	1364-6893
ISSN	0144-3615
DOI	10.1080/01443615.2019.1665631
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