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  1. Article ; Online: Endonuclease IV and T4 ligase enhanced detection of mutations in low abundance.

    Li, Bo / Wang, Chunyan

    The Analyst

    2024  Volume 149, Issue 4, Page(s) 1050–1054

    Abstract: We propose a mutant detection approach based on endonuclease IV and DNA ligase in combination with qPCR. The enzymes functioned cooperatively to facilitate PCR for low abundance DNA detection. We demonstrate that our approach can distinguish mutations as ...

    Abstract We propose a mutant detection approach based on endonuclease IV and DNA ligase in combination with qPCR. The enzymes functioned cooperatively to facilitate PCR for low abundance DNA detection. We demonstrate that our approach can distinguish mutations as low as 0.01%, indicating the potential application of this strategy in early cancer diagnosis.
    MeSH term(s) Deoxyribonuclease IV (Phage T4-Induced) ; Ligases ; Mutation ; DNA/genetics ; DNA/analysis ; DNA Ligases
    Chemical Substances Deoxyribonuclease IV (Phage T4-Induced) (EC 3.1.21.2) ; Ligases (EC 6.-) ; DNA (9007-49-2) ; DNA Ligases (EC 6.5.1.-)
    Language English
    Publishing date 2024-02-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 210747-8
    ISSN 1364-5528 ; 0003-2654
    ISSN (online) 1364-5528
    ISSN 0003-2654
    DOI 10.1039/d3an02083e
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Brain tumor detection based on a novel and high-quality prediction of the tumor pixel distributions.

    Sun, Yanming / Wang, Chunyan

    Computers in biology and medicine

    2024  Volume 172, Page(s) 108196

    Abstract: The work presented in this paper is in the area of brain tumor detection. We propose a fast detection system with 3D MRI scans of Flair modality. It performs 2 functions, predicting the gray level distribution and location distribution of the pixels in ... ...

    Abstract The work presented in this paper is in the area of brain tumor detection. We propose a fast detection system with 3D MRI scans of Flair modality. It performs 2 functions, predicting the gray level distribution and location distribution of the pixels in the tumor regions and generating tumor masks with pixel-wise precision. To facilitate 3D data analysis and processing, we introduce a 2D histogram presentation encompassing the gray-level distribution and pixel-location distribution of a 3D object. In the proposed system, specific 2D histograms highlighting tumor-related features are established by exploiting the left-right asymmetry of a brain structure. A modulation function, generated from the input data of each patient case, is applied to the 2D histograms to transform them into coarsely or finely predicted distributions of tumor pixels. The prediction result helps to identify/remove tumor-free slices. The prediction and removal operations are performed to the axial, coronal and sagittal slice series of a brain image, transforming it into a 3D minimum bounding box of its tumor region. The bounding box is utilized to finalize the prediction and generate a 3D tumor mask. The proposed system has been tested extensively with the data of more than 1200 patient cases in BraTS2018∼2021 datasets. The test results demonstrate that the predicted 2D histograms resemble closely the true ones. The system delivers also very good tumor detection results, comparable to those of state-of-the-art CNN systems with mono-modality inputs. They are reproducible and obtained at an extremely low computation cost and without need for training.
    MeSH term(s) Humans ; Brain ; Brain Neoplasms/diagnostic imaging ; Magnetic Resonance Imaging/methods ; Radiopharmaceuticals
    Chemical Substances Radiopharmaceuticals
    Language English
    Publishing date 2024-02-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 127557-4
    ISSN 1879-0534 ; 0010-4825
    ISSN (online) 1879-0534
    ISSN 0010-4825
    DOI 10.1016/j.compbiomed.2024.108196
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  3. Article ; Online: High resolution remote sensing image segmentation based on interval type-2 fuzzy theory

    WANG Chunyan

    Acta Geodaetica et Cartographica Sinica, Vol 48, Iss 1, Pp 132-

    2018  Volume 132

    Keywords Mathematical geography. Cartography ; GA1-1776
    Language Chinese
    Publishing date 2018-01-01T00:00:00Z
    Publisher Surveying and Mapping Press
    Document type Article ; Online
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  4. Article ; Online: The emerging role of clinical genetics in pediatric patients with chronic kidney disease.

    Dai, Rufeng / Wang, Chunyan / Shen, Qian / Xu, Hong

    Pediatric nephrology (Berlin, Germany)

    2024  

    Language English
    Publishing date 2024-03-19
    Publishing country Germany
    Document type Editorial
    ZDB-ID 631932-4
    ISSN 1432-198X ; 0931-041X
    ISSN (online) 1432-198X
    ISSN 0931-041X
    DOI 10.1007/s00467-024-06329-1
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  5. Article ; Online: Effects of lncRNA FEZF1-AS1 on proliferation, migration and invasion through regulating EZH2 of lung interstitial cells

    WANG Chunyan / WANG Ping / SONG Longfei / LIU Yongquan / MAN Jun

    Jichu yixue yu linchuang, Vol 44, Iss 1, Pp 43-

    2024  Volume 50

    Abstract: Objective To investigate the effects of long non-coding RNA FEZ family zinc finger 1 antisense RNA 1(lncRNA FEZF1-AS1) on enhancer of zeste homolog 2(EZH2) in regulation of proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of ...

    Abstract Objective To investigate the effects of long non-coding RNA FEZ family zinc finger 1 antisense RNA 1(lncRNA FEZF1-AS1) on enhancer of zeste homolog 2(EZH2) in regulation of proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of pulmonary interstitial cells and its mechanism. Methods The A549 cells human lung adenocarcinoma cell line were divided into control group and model group [model cells were induced into lung interstitial cells after being treated with transforming growth factor β1(TGF-β1)20 ng/mL for 48 h]. The protein expression of E-cadherin, N-cadherin and vimentin in each group was detected by Western blot. The expression of lncRNA FEZF1-AS1 and EZH2 in the two groups was detected by RT-qPCR. Cells in the transfection group were divided into si NC group,lncRNA FEZF1-AS1+OE vector group and si lncRNA FEZF1-AS1+OE EZH2 group. Cell proliferation was examined by CCK-8 method, cell migration was detected by cell scratch, and cell invasion was detected by Transwell assays. The protein expression of E-cadherin, N-cadherin, vimentin and EZH2 in each group was detected by Western blot. The direct binding effect of FEZF1-AS1 and EZH2 was determined by RNA immuno-precipitation (RIP). Results Compared with the control group, the protein expression level of E-cadherin in the model group was significantly decreased (P＜0.05),and the protein expression of N-cadherin and vimentin was significantly increased(P＜0.05). Compared with the control group, the expression level of lncRNA FEZF1-AS1 and EZH2 genes was significantly increased in the model group (P＜0.05). Compared with si NC group, the proliferation, migration and invasion ability of si lncRNA FEZF1-AS1+OE vector group were decreased, the expression of E-cadherin protein was increased while the expression of N-cadherin, vimentin and EZH2 was decreased(P＜0.05). Compared with si lncRNA FEZF1-AS1+OE vector group, the proliferation, invasion and migration of si lncRNA FEZF1-AS1+OE EZH2 group were increased (P＜0.05). E-cadherin expression was ...
    Keywords idiopathic pulmonary interstitial fibrosis|fez family zinc finger 1 antisense rna 1(fezf1-as1)|epithelial-mesenchymal transition(emt)|enhancer of zeste homolog 2(ezh2)|human lung adenocarcinoma cell line a549 ; Medicine ; R
    Subject code 616
    Language Chinese
    Publishing date 2024-01-01T00:00:00Z
    Publisher Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
    Document type Article ; Online
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  6. Article ; Online: Clinical Significance of Detection of Human Papilloma Virus DNA and E6/E7 mRNA for Cervical Cancer Patients.

    Yu, Tingting / Wang, Chunyan

    Cellular and molecular biology (Noisy-le-Grand, France)

    2022  Volume 67, Issue 6, Page(s) 155–159

    Abstract: This study aimed to explore the clinical significance of detection of human papillomavirus (HPV) DNA and E6/E7 mRNA for cervical cancer patients. For this purpose, a total of 300 patients with cervical lesions who performed the colposcopy examination for ...

    Abstract This study aimed to explore the clinical significance of detection of human papillomavirus (HPV) DNA and E6/E7 mRNA for cervical cancer patients. For this purpose, a total of 300 patients with cervical lesions who performed the colposcopy examination for the cervix between January 2018 and January 2020 were divided into three groups according to the level of cervical intraepithelial neoplasia (CIN): Low-level group (Group A, n = 101), high-level group (Group B, n = 149), cervical cancer group (Group C, n = 50) and gynecological inflammation group (Group D, n = 60). Tissue samples were collected from subjects above to perform the immunohistochemistry for E6/E7 protein and detection of HPV DNA and HPV E6/E7 mRNA. The results showed that HPV DNA copies and the positive rates of protein, DNA and mRNA of HPV E6/E7 in Groups A, B and C were significantly higher than those in Group D (all P<0.05), while no significant difference was identified in the comparison of the positive rate and copies of DNA among Group A, B and C (P> 0.05); in the Group A, B and C, patients had a higher positive rate of E6/E7 mRNA than those in the Group D (P<0.05), while in the Group B and C, the positive rate and copies of mRNA of HPV E6/E7 and HPV E6/E7 proteins were all higher than those in the Group A (P<0.05). In addition, the specificity of HPV E6/E7 mRNA was inferior to HPV DNA in the detection of the low-level intraepithelial neoplasia, but it performed better in the detection of the high-level intraepithelial neoplasia and cervical cancer. In general, HPV E6/E7 mRNA shows promising value in the detection of the development and progression of cervical cancer.
    MeSH term(s) DNA ; Female ; Humans ; Oncogene Proteins, Viral/genetics ; Papillomaviridae/genetics ; Papillomavirus Infections/pathology ; RNA, Messenger/genetics ; RNA, Viral/genetics ; Uterine Cervical Neoplasms/pathology
    Chemical Substances Oncogene Proteins, Viral ; RNA, Messenger ; RNA, Viral ; DNA (9007-49-2)
    Language English
    Publishing date 2022-02-27
    Publishing country France
    Document type Journal Article
    ZDB-ID 1161779-2
    ISSN 1165-158X ; 0145-5680
    ISSN (online) 1165-158X
    ISSN 0145-5680
    DOI 10.14715/cmb/2021.67.6.21
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    Zs.A 3812: Show issues Location:
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  7. Article ; Online: Analysis of the incidence and influencing factors of abdominal distension in postoperative lung cancer patients in ICU based on real-world data: a retrospective cohort study.

    Liu, Yan / Tang, Tingting / Wang, Chunyan / Wang, Chunmei / Zhu, Daxing

    BMC surgery

    2024  Volume 24, Issue 1, Page(s) 26

    Abstract: Background: Abdominal distension is a relatively common complication in postoperative lung cancer patients, which affects patients' early postoperative recovery to varying degrees. However, the current status of the incidence of abdominal distension in ... ...

    Abstract Background: Abdominal distension is a relatively common complication in postoperative lung cancer patients, which affects patients' early postoperative recovery to varying degrees. However, the current status of the incidence of abdominal distension in postoperative lung cancer patients and the affecting factors are not well understood. This study aims at exploring the incidence of abdominal distension in postoperative lung cancer patients in ICU based on real-world data and analyzing its influencing factors.
    Methods: A retrospective cohort study was conducted, encompassing patients who underwent lung cancer resections in the Lung Cancer Center of West China Hospital of Sichuan University from April 2020 to April 2021. Nevertheless, patients younger than 18 years and those whose information was limited in medical records were excluded. All data were obtained from the hospital HIS system. In this study, the influencing factors of abdominal distension were analyzed by univariate analysis and multiple logistic regression methods.
    Results: A total of 1317 patients met eligibility criteria, and were divided into the abdominal distended group and the non-distended group according to whether abdominal distension occurred after surgery. Abdominal distension occurred in a total of 182 cases(13.8%). The results of the univariate analysis showed that, compared with the non-distended group, the abdominal distended group had these features as follows: more women (P = 0.021), older (P = 0.000), lower BMI (P = 0.000), longer operation duration (P = 0.031), more patients with open thoracotomy (P = 0.000), more patients with pneumonectomy (p = 0.002), more patients with neoadjuvant chemotherapy (P = 0.000), more days of hospitalization on average (P = 0.000), and higher costs of hospitalization on average (P = 0.032). Multifactor logistic regression analysis showed that sex (OR = 0.526; 95% CI = 0.378 ~0.731), age (OR = 1.154; 95%CI = 1.022 ~1.304) and surgical approach (OR = 4.010; 95%CI = 2.781 ~5.781) were independent influencing factors for the occurrence of abdominal distension in patients after lung cancer surgery in ICU.
    Conclusions: The incidence of abdominal distension was high in postoperative lung cancer patients in ICU, and female, older and patients with open thoracotomy were more likely to experience abdominal distension.
    Trial registration: The study was approved by the Chinese Clinical Trials Registry (registration number was ChiCTR2200061370).
    MeSH term(s) Female ; Humans ; Incidence ; Intensive Care Units ; Lung Neoplasms/surgery ; Postoperative Complications/epidemiology ; Retrospective Studies ; Risk Factors ; Male
    Language English
    Publishing date 2024-01-18
    Publishing country England
    Document type Journal Article
    ZDB-ID 2050442-1
    ISSN 1471-2482 ; 1471-2482
    ISSN (online) 1471-2482
    ISSN 1471-2482
    DOI 10.1186/s12893-024-02317-2
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  8. Article ; Online: 2,4,6-trinitrotoluene causes mitochondrial toxicity in Caenorhabditis elegans by affecting electron transport.

    Zhou, Yanping / Wang, Chunyan / Nie, Yaguang / Wu, Lijun / Xu, An

    Environmental research

    2024  Volume 252, Issue Pt 1, Page(s) 118820

    Abstract: As a typical energetic compound widely used in military activities, 2,4,6-trinitrotoluene (TNT) has attracted great attention in recent years due to its heavy pollution and wide distribution in and around the training facilities, firing ranges, and ... ...

    Abstract As a typical energetic compound widely used in military activities, 2,4,6-trinitrotoluene (TNT) has attracted great attention in recent years due to its heavy pollution and wide distribution in and around the training facilities, firing ranges, and demolition sites. However, the subcellular targets and the underlying toxic mechanism of TNT remain largely unknown. In this study, we explored the toxic effects of TNT biological reduction on the mitochondrial function and homeostasis in Caenorhabditis elegans (C. elegans). With short-term exposure of L4 larvae, 10-1000 ng/mL TNT reduced mitochondrial membrane potential and adenosine triphosphate (ATP) content, which was associated with decreased expression of specific mitochondrial complex involving gas-1 and mev-1 genes. Using fluorescence-labeled transgenic nematodes, we found that fluorescence expression of sod-3 (muls84) and gst-4 (dvls19) was increased, suggesting that TNT disrupted the mitochondrial antioxidant defense system. Furthermore, 10 ng/mL TNT exposure increased the expression of the autophagy-related gene pink-1 and activated mitochondrial unfolded protein response (mt UPR), which was indicated by the increased expression of mitochondrial stress activated transcription factor atfs-1, ubiquitin-like protein ubl-5, and homeobox protein dve-1. Our findings demonstrated that TNT biological reduction caused mitochondrial dysfunction and the development of mt UPR protective stress responses, and provided a basis for determining the potential risks of energetic compounds to living organisms.
    Language English
    Publishing date 2024-03-29
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 205699-9
    ISSN 1096-0953 ; 0013-9351
    ISSN (online) 1096-0953
    ISSN 0013-9351
    DOI 10.1016/j.envres.2024.118820
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    Zs.A 726: Show issues Location:
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  9. Article ; Online: Effects of evidence-based nursing care interventions on wound pain and wound complications following surgery for finger tendon injury.

    Zhang, Xin-Lan / Wang, Chun-Yan / Pan, Liu-Liu / Li, Yu-Jie

    International wound journal

    2024  Volume 21, Issue 3, Page(s) e14818

    Abstract: We conducted this study aimed to examine the impact of evidence-based nursing interventions on postoperative wound pain and complications after surgery for finger tendon injury. A total of 86 patients treated for finger tendon injuries at our hospital ... ...

    Abstract We conducted this study aimed to examine the impact of evidence-based nursing interventions on postoperative wound pain and complications after surgery for finger tendon injury. A total of 86 patients treated for finger tendon injuries at our hospital from January 2021 to October 2023 were selected and randomly divided into an experimental group and a control group. The control group received conventional nursing care, while the experimental group received evidence-based nursing interventions. The study compared the postoperative wound pain intensity, incidence of complications and patient satisfaction with nursing care between the two groups. The analysis revealed that compared with conventional care, evidence-based nursing interventions significantly reduced the level of wound pain (p = 0.034) and the incidence of complications (4.65% vs. 18.60%, p = 0.043). It also increased patient satisfaction with the nursing care (97.67% vs. 83.72%, p = 0.026). The study indicates that the application of evidence-based nursing interventions for patients with finger tendon injuries can reduce postoperative wound pain, decrease the incidence of complications and enhance patient satisfaction with nursing care.
    MeSH term(s) Humans ; Evidence-Based Nursing ; Finger Injuries/surgery ; Fingers ; Pain, Postoperative/therapy ; Tendon Injuries/surgery
    Language English
    Publishing date 2024-03-05
    Publishing country England
    Document type Journal Article ; Randomized Controlled Trial
    ZDB-ID 2170920-8
    ISSN 1742-481X ; 1742-4801
    ISSN (online) 1742-481X
    ISSN 1742-4801
    DOI 10.1111/iwj.14818
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  10. Article ; Online: DNA Windmill Probe for Multiplexed mRNA Detection and Cell Type Discrimination.

    Zhao, Yu / Li, Zhihao / Li, Bo / Wang, Chunyan

    Chemistry (Weinheim an der Bergstrasse, Germany)

    2023  Volume 29, Issue 47, Page(s) e202301300

    Abstract: Accurate cancer diagnosis especially early diagnosis is of great importance for prompt therapy and elevated survival rate. mRNAs are widely used as biomarkers for cancer identification and treatment. mRNA expression levels are highly associated with ... ...

    Abstract Accurate cancer diagnosis especially early diagnosis is of great importance for prompt therapy and elevated survival rate. mRNAs are widely used as biomarkers for cancer identification and treatment. mRNA expression levels are highly associated with cancer stage and malignant progression. Nevertheless, single type mRNA detection is insufficient and unreliable. Herein, we developed a DNA nano-windmill probe for in situ multiplexed mRNAs detection and imaging in this paper. The probe is designed to simultaneously target four types of mRNA through wind blades. Importantly, recognition of targets is independent from each other, which further facilitate cell type discrimination. The probe can specifically distinguish cancer cell lines from normal cells. In addition, it can identify changes in mRNA expression levels in living cells. The current strategy enriches the toolbox for improving the accuracy of cancer diagnosis and therapeutic solutions.
    MeSH term(s) RNA, Messenger/genetics ; DNA Probes ; Cell Line, Tumor ; DNA
    Chemical Substances RNA, Messenger ; DNA Probes ; DNA (9007-49-2)
    Language English
    Publishing date 2023-07-28
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1478547-X
    ISSN 1521-3765 ; 0947-6539
    ISSN (online) 1521-3765
    ISSN 0947-6539
    DOI 10.1002/chem.202301300
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