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Article ; Online: Acquisition of different transcriptional shear mRNA and biological function of porcine interleukin 18 binding protein in PRRSV infection.

Zhao, Hong-Zhe / Liu, Chun-Yu / Song, Qian-Jin / Guo, Hao / Wen, Yong-Jun / Wang, Feng-Xue

2024 , Page(s) e0064024

Abstract: Interleukin-18 binding protein (IL-18BP), a natural regulator molecule of the pro-inflammatory cytokine interleukin-18 (IL-18), plays an important role in regulating the expression of the cellular immunity factor interferon-γ (IFN-γ). In a previous RNA- ... ...

Abstract	Interleukin-18 binding protein (IL-18BP), a natural regulator molecule of the pro-inflammatory cytokine interleukin-18 (IL-18), plays an important role in regulating the expression of the cellular immunity factor interferon-γ (IFN-γ). In a previous RNA-seq analysis of porcine alveolar macrophages (PAM) infected with the TIM and TJ strains of porcine reproductive and respiratory syndrome virus (PRRSV), we unexpectedly found that the mRNA expression of porcine interleukin 18-binding protein (pIL-18BP) in PAM cells infected with the TJM strain was significantly higher than that infected with the TJ strain. Studies have shown that human interleukin-18 binding protein (hIL-18bp) plays an important role in regulating cellular immunity in the course of the disease. However, there is a research gap on pIL-18BP. At the same time, PRRSV infection in pigs triggers weak cellular immune response problems. To explore the expression and the role of pIL-18BP in the cellular immune response induced by PRRSV, we strived to acquire the pIL-18BP gene from PAM or peripheral blood mononuclear cell (PBMC) with RT-PCR and sequencing. Furthermore, pIL-18BP and pIL-18 were both expressed prokaryotically and eukaryotically. The colocalization and interaction based on recombinant pIL-18BP and pIL-18 on cells were confirmed
Language	English
Publishing date	2024-05-10
Publishing country	United States
Document type	Journal Article
ZDB-ID	2557172-2
ISSN	2150-7511 ; 2161-2129
ISSN (online)	2150-7511
ISSN	2161-2129
DOI	10.1128/mbio.00640-24
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Article ; Online: Prediction Model of Agricultural Non-point Source Water Pollution Based on Grey Correlation Method

R. Wang, F. X. Yang and G. M. Qu

Nature Environment and Pollution Technology, Vol 20, Iss 2, Pp 633-

2021 Volume 641

Abstract: Accurate prediction of non-point source water pollution is conducive to the prevention and control of rural water pollution. To improve the prediction accuracy of agricultural non-point source water pollution and achieve timely prevention and control, a ... ...

Abstract	Accurate prediction of non-point source water pollution is conducive to the prevention and control of rural water pollution. To improve the prediction accuracy of agricultural non-point source water pollution and achieve timely prevention and control, a prediction model of agricultural non-point source water pollution based on the grey correlation method is designed. According to the historical data of agricultural non-point source water pollution, the influencing factors of water pollution degree are determined by using the grey correlation method, and standardized pretreatment is carried out. The pretreatment results are transformed into function expression forms, and the original sequences of different influencing factors are generated, which are brought into the function table to achieve the results, and the whitening differential equation is constructed to measure the concentration of agricultural non-point source water pollution. The structure design of the prediction model of agricultural non-point source water pollution can realize the prediction of agricultural non-point source water pollution. The results show that the prediction model of agricultural non-point source water pollution based on the grey correlation method has high prediction accuracy and small prediction error.
Keywords	grey relation method ; agricultural non-point source ; pollution ; prediction model ; Environmental effects of industries and plants ; TD194-195 ; Science (General) ; Q1-390
Subject code	333
Language	English
Publishing date	2021-06-01T00:00:00Z
Publisher	Technoscience Publications
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: [The efficacy of chemotherapy re-challenge in third-line setting for metastatic colorectal cancer patients: a real-world study].

Duan, J J / Ning, T / Bai, M / Zhang, L / Li, H L / Liu, R / Ge, S H / Wang, X / Yang, Y C / Ji, Z / Wang, F X / Sun, Y S / Ba, Y / Deng, T

Zhonghua zhong liu za zhi [Chinese journal of oncology

2023 Volume 45, Issue 11, Page(s) 967–972

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Humans ; Irinotecan/therapeutic use ; Oxaliplatin/therapeutic use ; Colorectal Neoplasms/pathology ; Retrospective Studies ; Fluorouracil ; Colonic Neoplasms/chemically induced ; Rectal Neoplasms/drug therapy ; Antineoplastic Combined Chemotherapy Protocols/adverse effects ; Camptothecin/adverse effects
Chemical Substances	Irinotecan (7673326042) ; Oxaliplatin (04ZR38536J) ; Fluorouracil (U3P01618RT) ; Camptothecin (XT3Z54Z28A)
Language	Chinese
Publishing date	2023-11-16
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	603424-x
ISSN	0253-3766
ISSN	0253-3766
DOI	10.3760/cma.j.cn112152-20220901-00591
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Article ; Online: Targeting the HMGA2 oncogene by miR-498 inhibits non-small cell lung cancer biological behaviors.

Gao, N / Wang, F-X / Wang, G / Zhao, Q-S

European review for medical and pharmacological sciences

2018 Volume 22, Issue 6, Page(s) 1693–1699

Abstract: Objective: Previous study reported that miR-498 served as a tumor suppressor in non-small cell lung cancer (NSCLC), but the underlying mechanism remains largely unknown. The aim of this study is to investigate the role of miR-498 and its target gene ... ...

Abstract	Objective: Previous study reported that miR-498 served as a tumor suppressor in non-small cell lung cancer (NSCLC), but the underlying mechanism remains largely unknown. The aim of this study is to investigate the role of miR-498 and its target gene HMGA2 in NSCLC progression. Patients and methods: The expression of miR-498 was assessed in clinical NSCLC specimens and cell lines using RT-PCR. Overexpression of miR-498 and transfection of pLenti-HMGA2 were performed in A549 cells. Cell proliferation, apoptosis, migration, and invasion were determined using cell counting kit-8 (CCK-8) assay, clone formation assay, flow cytometry, and transwell assay, respectively. Luciferase reporter assays were performed to analyze the regulation of putative target of miR-498. Western blot was used to detect the levels of HMGA2 in A549 cells. Results: MiR-498 was found to be down-regulated in NSCLC tissues and cell lines. After miR-498 mimics transfection, cell proliferation, migration, and invasion were significantly suppressed in the NSCLC cells. Mechanistically, bioinformatic analysis predicted that miR-498 may target the 3'-UTR of HMGA2 and suppressed its translation, and was further confirmed by luciferase assay. Furthermore, restoration of HMGA2 expression completely rescued the inhibitory effect of miR-498 in NSCLC cells. Conclusions: This paper revealed that miR-498 may serve as a tumor suppressor in NSCLC through targeting HMGA2, suggesting that miR-498 could represent a novel target for effective therapies.
MeSH term(s)	A549 Cells ; Apoptosis/genetics ; Carcinoma, Non-Small-Cell Lung/genetics ; Carcinoma, Non-Small-Cell Lung/pathology ; Cell Movement/genetics ; Cell Proliferation/genetics ; Down-Regulation ; Female ; Genes, Tumor Suppressor ; HMGA2 Protein/genetics ; Humans ; Lung Neoplasms/genetics ; Lung Neoplasms/pathology ; MicroRNAs/genetics ; Oncogenes ; Transfection
Chemical Substances	HMGA2 Protein ; HMGA2 protein, human ; MIRN498 microRNA, human ; MicroRNAs
Language	English
Publishing date	2018-04-08
Publishing country	Italy
Document type	Journal Article
ZDB-ID	605550-3
ISSN	2284-0729 ; 1128-3602 ; 0392-291X
ISSN (online)	2284-0729
ISSN	1128-3602 ; 0392-291X
DOI	10.26355/eurrev_201803_14582
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Article: [Inhibition of the TGF-β/Smad3 signaling pathway by SB431542: A study of the intervention effect of SB431542 on silicotic fibrosis in rats].

Zhou, Q W / Gao, F Y / Tian, D / Liu, H R / Wang, F X

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases

2019 Volume 37, Issue 11, Page(s) 801–805

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Animals ; Benzamides/pharmacology ; Benzamides/therapeutic use ; Dioxoles/pharmacology ; Dioxoles/therapeutic use ; Fibrosis/drug therapy ; Gene Expression Regulation/drug effects ; Rats ; Rats, Sprague-Dawley ; Signal Transduction/drug effects ; Smad3 Protein/genetics ; Transforming Growth Factor beta/genetics
Chemical Substances	4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide ; Benzamides ; Dioxoles ; Smad3 Protein ; Transforming Growth Factor beta
Language	Chinese
Publishing date	2019-12-11
Publishing country	China
Document type	Journal Article
ISSN	1001-9391
ISSN	1001-9391
DOI	10.3760/cma.j.issn.1001-9391.2019.11.001
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Article: Reproductive allocation and fruit-set pattern in the euhalophyte Suaeda salsa in controlled and field conditions

Wang, F. X / C. H. Yin / C. Y. Tian / J. Song / Q. Li / Y. P. Song

Plant biosystems. 2018 July 4, v. 152, no. 4

2018

Abstract: Reproductive allocation and fruit-set pattern are important characteristics of halophytes, and these characteristics were investigated in euhalophyte Suaeda salsa (S. salsa). The plants comprised of those treated with 1 mM (control) or 500 mM (high ... ...

Abstract	Reproductive allocation and fruit-set pattern are important characteristics of halophytes, and these characteristics were investigated in euhalophyte Suaeda salsa (S. salsa). The plants comprised of those treated with 1 mM (control) or 500 mM (high salinity) of NaCl for 120 days in a glasshouse and those collected in the field. In controlled conditions, high salinity increased reproductive allocation and the number of fruits and seeds in each node of the branches. In field conditions, reproductive allocation and the number of fruits and brown seeds in S. salsa at the upper tide line were higher than at the lower tide line. There were more fruits and seeds distributed in the central part than those in the base or top of branches in both controlled and field conditions. Soil organic matter, total nitrogen and total phosphorus content at the upper tide line were lower than at the lower tide line. An opposite trend could be observed in the total soluble salt, Na+ and Cl− content in soil. In conclusion, the fruit-set pattern of S. salsa may be affected by both resource constraints and resource allocation. Reproductive allocation and fruit-set pattern may ensure the establishment of S. salsa in high saline and low nutrient environments.
Keywords	branches ; fruit set ; fruits ; greenhouses ; halophytes ; nitrogen content ; phosphorus ; resource allocation ; salinity ; seeds ; sodium ; sodium chloride ; soil ; soil organic matter ; Suaeda
Language	English
Dates of publication	2018-0704
Size	p. 749-758.
Publishing place	Taylor & Francis
Document type	Article
ISSN	1724-5575
DOI	10.1080/11263504.2017.1330776
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Mechanism underlying the bio-effects of an electromagnetic field based on the Huang-Ferrell model.

Geng, D Y / Hu, G / Wang, L / Jia, N / Wang, F X

Genetics and molecular research : GMR

2016 Volume 15, Issue 2

Abstract: To understand the beneficial and harmful bio-effects of extremely low frequency electromagnetic fields, we studied the MAPK/ERK signaling pathway based on the Huang-Ferrell model. The sensitivity analysis method was used to study the influence of the ... ...

Abstract	To understand the beneficial and harmful bio-effects of extremely low frequency electromagnetic fields, we studied the MAPK/ERK signaling pathway based on the Huang-Ferrell model. The sensitivity analysis method was used to study the influence of the model parameters on the activity of ERK, and to further investigate the key biochemical reactions and proteins. The results of the simulation show that an increase in the reaction rate of MAPK/ERK kinase had little effect on ERK activation and the steady-state molecular number. However, a decrease in the reaction rate of MAPK/ERK kinase significantly affected the trigger time of ERK activation and decreased the steady-state molecular number. Together with the biological significance of ERK activity, our findings indicate that the effects of electromagnetic fields are a result of the decrease in the reaction rate of MAPK/ERK kinase, which eventually determines whether these effects cause physical damage or are beneficial in treatment.
MeSH term(s)	Animals ; Electromagnetic Fields/adverse effects ; Extracellular Signal-Regulated MAP Kinases/metabolism ; Humans ; MAP Kinase Signaling System/radiation effects ; Models, Theoretical
Chemical Substances	Extracellular Signal-Regulated MAP Kinases (EC 2.7.11.24)
Language	English
Publishing date	2016-06-27
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2114039-X
ISSN	1676-5680 ; 1676-5680
ISSN (online)	1676-5680
ISSN	1676-5680
DOI	10.4238/gmr.15028796
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Article: [Efficacy of antiretroviral therapy and influencing factors for people living with HIV/AIDS in designated hospitals in Harbin].

Zhao, P Y / Yu, X / Yang, K / Feng, S Y / Wang, F X / Wang, B Y

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi

2016 Volume 37, Issue 5, Page(s) 634–637

Abstract: Objective: To understand the efficacy of antiretroviral therapy for people living with HIV/AIDS and influencing factors; and provide evidence to improve the treatment of HIV infection and AIDS for the better life of the patients.: Methods: A cross ... ...

Abstract	Objective: To understand the efficacy of antiretroviral therapy for people living with HIV/AIDS and influencing factors; and provide evidence to improve the treatment of HIV infection and AIDS for the better life of the patients. Methods: A cross sectional study was conducted in designated AIDS hospitals in Harbin. A questionnaire was used to collect the information of the patients receiving treatment in these hospitals. The statistical analysis was done with software SAS 9.2 and Excel 2010. Univariate analysis was performed with t test and multivariate analysis was performed with ordinal logistic regression model. Wilcoxon ranks sum test was conducted to compare the CD4(+) T lymphocyte counts. Results: The number of the patients receiving antiretroviral therapy was in increase in recent years. The HIV infection route was mainly homosexual contact. The CD4(+)T lymphocyte count of the patients increased at different levels after ≥6 months treatment(P<0.01). Household income(P<0.05), adherence to treatment plan or not(P<0.05), social relationship(P< 0.05), concern of economic cost(P<0.01)medication compliance(P<0.01)and initial level of CD4(+) T lymphocyte(P<0.01)were the influencing factors for antiretroviral therapy efficacy. Conclusion: In designated hospitals in Harbin, the number of the patients receiving HIV antiretroviral therapy kept to increase and the efficacy of the treatment was obvious.
MeSH term(s)	Acquired Immunodeficiency Syndrome ; Antiretroviral Therapy, Highly Active/methods ; China ; Communicable Diseases ; Cross-Sectional Studies ; HIV Infections/drug therapy ; HIV Infections/psychology ; HIV Infections/virology ; Hospitals ; Humans ; Medication Adherence/statistics & numerical data ; Quality of Life ; T-Lymphocytes ; Treatment Outcome
Language	Chinese
Publishing date	2016-05
Publishing country	China
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	645026-x
ISSN	0254-6450
ISSN	0254-6450
DOI	10.3760/cma.j.issn.0254-6450.2016.05.009
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Article ; Online: Development of an indirect enzyme-linked immunosorbent assay (ELISA) to differentiate antibodies against wild-type porcine reproductive and respiratory syndrome from the vaccine strain TJM-F92 based on a recombinant Nsp2 protein.

Wang, X X / Wang, F X / Li, Z G / Wen, Y J / Wang, X / Song, N / Wu, H

Journal of virological methods

2018 Volume 251, Page(s) 151–154

Abstract: An accurate ELISA method to differentiate pigs infected with wild-type porcine reproductive and respiratory syndrome (PRRSV) strains from vaccinated ones would help to monitor PRRSV vaccination compliance. The recombinant protein GST-d120aa derived from ... ...

Abstract	An accurate ELISA method to differentiate pigs infected with wild-type porcine reproductive and respiratory syndrome (PRRSV) strains from vaccinated ones would help to monitor PRRSV vaccination compliance. The recombinant protein GST-d120aa derived from the continuous deletion of 120 amino acids in the non-structural protein 2 region of the modified-live vaccine strain TJM-F92 was used to develop an indirect enzyme-linked immunosorbent assay (d120-ELISA) for differentiating serum antibodies against TJM-F92 from other PRRSV strains. At the optimized cut-off value which was calculated at an S/P of 0.25, it yielded a sensitivity of 90.7% and a specificity of 95.1%. Cross-reactivity tests suggested that the d120-ELISA was PRRSV-specific. Coefficient of variations of the repeatability tests ranged between 1.41-17.02%. The results suggest that the d120-ELISA is suitable for differentiating animals infected with wild-type strains from those immunized with MLV TJM-F92.
Language	English
Publishing date	2018-01
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	8013-5
ISSN	1879-0984 ; 0166-0934
ISSN (online)	1879-0984
ISSN	0166-0934
DOI	10.1016/j.jviromet.2017.09.001
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Article: Effects of stocking density on behavior, productivity, and comfort indices of lactating dairy cows

Wang, F.X / A. Azarfar / D.F. Shao / S.L. Li / Y.J. Wang / Z.J. Cao

American Dairy Science Association Journal of dairy science. 2016 May, v. 99, no. 5

2016

Abstract: The objective of this study was to investigate the effects of different stocking densities of 82 (0.82 cows per freestall and feed bin), 100, and 129% on behavior, productivity, and comfort indices of lactating Holstein dairy cows. Twenty-seven lactating ...

Abstract	The objective of this study was to investigate the effects of different stocking densities of 82 (0.82 cows per freestall and feed bin), 100, and 129% on behavior, productivity, and comfort indices of lactating Holstein dairy cows. Twenty-seven lactating cows (15 primiparous and 12 multiparous) were assigned to 1 of the 3 treatments, which were balanced for parity, milk yield, days in milk, and body weight in a 3×3 Latin square design with 14-d periods. After 7 d of adaptation to the treatments, lying time and bouts were recorded at 1-min intervals for 3 d, DMI and feeding time were monitored electronically by feed bins, and rumination time was quantified at 2-h periods for 5 d during each period. The cow comfort index, stall standing index, stall perching index, and stall use index (SUI) were calculated using 10-min scan samples of video recording from d 8 to 10 of each period. Milk yield was recorded from d 8 to 12 and milk composition was determined from composite samples on d 12 in each period. Daily lying time, lying bouts, and bout duration did not differ among the stocking densities. The ratio of lying time ≥12 h/d (the number of cows with daily lying time ≥12 h/d divided by number of cows per pen) was higher for cows housed at 82% stocking density compared with those housed at 100% stocking density, with stocking density of 129% intermediate. Hourly lying time was lower at 100% stocking density compared with 82 and 129% stocking densities during the peak period (2300–0400 h), determined based on diurnal pattern of lying time. Daily dry matter intake, feeding time, and feeding rate were not affected by stocking density. After morning milking, dry matter intake and feeding time was reduced at 129 versus 82% stocking density during peak feeding time (0600–0800 h), determined based on diurnal patterns of feeding behavior. Stocking density had no effect on rumination time, milk yield and milk composition. The ratio of SUI ≥85% (mean of the number of SUI ≥85% divided by the number of SUI at 10-min scan samples during a 24-h period) was lower at 129 versus 82% stocking density, with stocking density of 100% intermediate. During peak lying time after evening milking (2300–0400 h), both cow comfort index and SUI were higher at 129 than at 100% stocking density. The SUI was lower 2h after morning milking (0800–0900 h) for cows housed at 129% compared with those housed at 82 and 100% stocking densities. In conclusion, when compared with 100% stocking density, understocking contributed to natural behaviors of cows that including lying, feeding, and rumination behavior, whereas overstocking did not cause negative effect on behavior, productivity, and comfort indices of cows in this study.
Keywords	adverse effects ; body weight ; dairy cows ; diurnal variation ; dry matter intake ; feeding behavior ; free stalls ; Holstein ; lactation ; milk composition ; milk yield ; milking ; rumination ; stocking rate
Language	English
Dates of publication	2016-05
Size	p. 3709-3717.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	242499-x
ISSN	1525-3198 ; 0022-0302
ISSN (online)	1525-3198
ISSN	0022-0302
DOI	10.3168/jds.2015-10098
Database	NAL-Catalogue (AGRICOLA)

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