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Article ; Online: Interference-free quantitation of aromatic amino acids in two complex systems by three-way calibration with ultraviolet-visible spectrophotometer: Exploration of trilinear decomposition of spectrum-pH data.

Wang, Fu-Xin / Chen, Yang / Liang, Yan-Mei / Yang, Min / Kang, Chao

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

2022 Volume 290, Page(s) 122293

Abstract: Aromatic amino acids play an extremely important role in life activities and participate in many biological processes. Their concentration levels are associated with a variety of diseases, such as phenylketonuria and colorectal cancer. Therefore, the ... ...

Abstract	Aromatic amino acids play an extremely important role in life activities and participate in many biological processes. Their concentration levels are associated with a variety of diseases, such as phenylketonuria and colorectal cancer. Therefore, the quantification of aromatic amino acids is an important task. In the present work, a novel and rapid three-way analytical method was proposed to detect the levels of aromatic amino acids in prostate cancer cells (PC3 cells) and Dulbecco's modified minimal essential medium (DMEM cell culture), by using the affordable ultraviolet-visible spectrophotometer. First, spectrum-pH second-order data were designed per sample; Second, properties of the resulted spectrum-pH-sample three-way data were investigated by utilizing the parallel factor analysis (PARAFAC), alternating trilinear decomposition (ATLD), and constrained alternating trilinear decomposition (CATLD) algorithms, and a flexible scanning approach for determining the constraint parameters of CATLD was proposed; Third, a three-way calibration method based on the CATLD algorithm with the proposed scanning approach was developed for interference-free quantification of aromatic amino acids in these systems. The average relative predictive errors of validation (ARPEV) for phenylalanine, tyrosine, and tryptophan were 1.4%, 3.0%, and 0.7% in prostate cancer cells, and ARPEV for phenylalanine, tyrosine, and tryptophan were 4.1%, 1.2%, and 0.7% in DMEM cell culture. The predicted contents of tyrosine and tryptophan in DMEM cell culture were 64.2 ± 2.9 μg mL
MeSH term(s)	Amino Acids, Aromatic ; Calibration ; Tryptophan ; Chromatography, High Pressure Liquid/methods ; Algorithms ; Tyrosine ; Phenylalanine ; Hydrogen-Ion Concentration
Chemical Substances	Amino Acids, Aromatic ; Tryptophan (8DUH1N11BX) ; Tyrosine (42HK56048U) ; Phenylalanine (47E5O17Y3R)
Language	English
Publishing date	2022-12-31
Publishing country	England
Document type	Journal Article
ZDB-ID	210413-1
ISSN	1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425
ISSN (online)	1873-3557
ISSN	0370-8322 ; 0584-8539 ; 1386-1425
DOI	10.1016/j.saa.2022.122293
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Article ; Online: Selectivity Quantification with the Fluorescent Quantitative Model-Assisted Semi-Selective Probe (Carbon Dots): Accurate Determination of Chlortetracycline in Aqueous Environments with Interference.

Chen, Yang / Yan, Xiu-Fang / Bin, Jun / Liang, Yan-Mei / Wang, Fu-Xin / Yang, Min / Kang, Chao

Applied spectroscopy

2023 Volume 77, Issue 6, Page(s) 636–651

Abstract: Probes such as carbon dots (C-dots) have extensive and important applications in the quantitative analysis of complex biological and environmental systems. However, the development of probes is often hindered by incomplete selectivity, i.e., a probe that ...

Abstract	Probes such as carbon dots (C-dots) have extensive and important applications in the quantitative analysis of complex biological and environmental systems. However, the development of probes is often hindered by incomplete selectivity, i.e., a probe that responds to one substance is also prone to respond to coexisting structurally similar substances. Therefore, the above dilemma often leads to be developed as semi-selective probes, so that the development of probes is abandoned halfway. This work shows how a semi-selective probe can enhance selectivity by combining a proper multivariate calibration model. Primarily, we developed a semi-selective fluorescent probe that responded to tetracyclines (TCs) with discarded tobacco leaves. Then, we introduced the multivariate quantitative fluorescence model (QFM) to enhance its selectivity and solve the problem of fluorescence spectral shift. For the determination of chlortetracycline (CTC) with this semi-selective C-dots probe in mineral and lake water samples and compared to the traditional quantitative model, the introduced QFM resulted in an average relative predictive error (ARPE) in mineral water spiked samples decreased from 57.1 to 5.6%, which reduced the ARPE in the lake water spiked samples from 18.1 to 4.7%. The above results show that the QFM-assisted semi-selective probe C-dots strategy (QFM
MeSH term(s)	Chlortetracycline/analysis ; Fluorescent Dyes ; Carbon ; Spectrometry, Fluorescence/methods ; Quantum Dots ; Water ; Limit of Detection
Chemical Substances	Chlortetracycline (WCK1KIQ23Q) ; Fluorescent Dyes ; Carbon (7440-44-0) ; Water (059QF0KO0R)
Language	English
Publishing date	2023-05-07
Publishing country	United States
Document type	Journal Article
ISSN	1943-3530
ISSN (online)	1943-3530
DOI	10.1177/00037028231167177
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Article ; Online: The cytokine profiles in follicular fluid and reproductive outcomes in women with endometriosis.

Han, Mu-Tian / Cheng, Wei / Zhu, Rui / Wu, Hui-Hua / Ding, Jie / Zhao, Nan-Nan / Li, Hong / Wang, Fu-Xin

American journal of reproductive immunology (New York, N.Y. : 1989)

2022 Volume 89, Issue 6, Page(s) e13633

Abstract: Problem: Endometriosis patients undergoing in vitro fertilization-embryo transfer (IVF-ET) treatment suffer from poor oocyte quality, a reduced likelihood of the fertilization rate, and low embryo quality. The dysregulation of immune cells and cytokine ... ...

Abstract	Problem: Endometriosis patients undergoing in vitro fertilization-embryo transfer (IVF-ET) treatment suffer from poor oocyte quality, a reduced likelihood of the fertilization rate, and low embryo quality. The dysregulation of immune cells and cytokine profiles in the follicular fluid (FF) may play an important role in the competence of the oocyte and the development of the embryo, but the mechanism remains largely unknown. Method of study: A total of 40 proved advanced staged endometriosis patients were enrolled in this study. The pregnancy results were followed until all the embryos collected by the first oocyte retrieval cycle were used up. The immune cells subtypes in FF and serum collected on the day of oocyte retrieval were detected by flow cytometry and 27 cytokines were determined using the Bio-Plex Pro Human Cytokine 27-Plex Immunoassay. The specific effect of cytokine on the gene expression of human granulosa cells was determined by RT-qPCR. Results: The fertilization rate and the cumulative live birth rate were significantly lower in the endometriosis group. The ratio of CD4 Conclusion: These results suggest that alterations of the lymphocyte subsets and cytokines in women with advanced endometriosis may have an impact on the oocyte development and resulting in poorer IVF outcomes.
MeSH term(s)	Pregnancy ; Humans ; Female ; Follicular Fluid/metabolism ; Endometriosis/metabolism ; Infertility, Female/metabolism ; CD8-Positive T-Lymphocytes/metabolism ; Chemokine CXCL10/metabolism ; Fertilization in Vitro/methods ; Cytokines/metabolism
Chemical Substances	Chemokine CXCL10 ; Cytokines
Language	English
Publishing date	2022-10-26
Publishing country	Denmark
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	604542-x
ISSN	1600-0897 ; 0271-7352 ; 8755-8920 ; 1046-7408
ISSN (online)	1600-0897
ISSN	0271-7352 ; 8755-8920 ; 1046-7408
DOI	10.1111/aji.13633
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Article: The Role of Traditional Chinese Formula Ding-Kun Pill (DKP) in Expected Poor Ovarian Response Women (POSEIDON Group 4) Undergoing

Song, Jing-Yan / Gao, Dan-Dan / Cao, Xian-Ling / Xiang, Shan / Chen, Yan-Hua / Teng, Yi-Li / Li, Xiu-Fang / Liu, Hai-Ping / Wang, Fu-Xin / Zhang, Bin / Xu, Li-Hua / Zhou, Li / Huang, Xiang-Hong / Sun, Zhen-Gao

Frontiers in endocrinology

2021 Volume 12, Page(s) 675997

Abstract: Objective: The primary objective of the study was to assess traditional Chinese formula DKP supplementation in terms of efficacy and safety on reproductive outcomes of expected poor ovarian responder (POR, POSEIDON Group 4) undergoing : Design setting ...

Abstract	Objective: The primary objective of the study was to assess traditional Chinese formula DKP supplementation in terms of efficacy and safety on reproductive outcomes of expected poor ovarian responder (POR, POSEIDON Group 4) undergoing Design setting and participants: Women eligible for IVF-ET were invited to participate in this randomized, double-blind, placebo-controlled, superiority trial at academic fertility centers of ten public hospitals in Chinese Mainland. A total of 462 patients (35-44 years) equally divided between DKP and placebo groups with antral follicle count (AFC) <5 or anti-müllerian hormone (AMH) <1.2 ng/ml were randomized. Interventions: All participants were given DKP or 7 g placebo twice daily on the previous menstrual cycle day 5 until oocyte retrieval, which took approximately 5 to 6 weeks. Main outcome measure: The primary outcome was the ongoing pregnancy defined as more than 20 gestational weeks of an intrauterine living fetus confirmed by pelvic ultrasonography. Results: Demographic characteristics were equally distributed between the study populations. Intention-to-treat (ITT) analysis revealed that ongoing pregnancy rate (OPR) was not significantly different between DKP and placebo groups [26.4% (61/231) versus 24.2% (56/231); relative risk (RR) 1.09, 95% confidence interval (CI) 0.80 to 1.49, Conclusion: This well-designed randomized controlled trial (RCT) offers new high-quality evidence to supplement existing retrospective literature concerning DKP performance in expected PORs. DKP could be recommended as a safe and natural remedy for expected PORs (aged 35-37 years) who fulfill the POSEIDON group 4 criteria. However, additional interventional clinical studies are undoubtedly required to be conducted in the future to validate this hypothesis. Clinical trial registration: www.chictr.org.cn, identifier ChiCTR1900026614.
MeSH term(s)	Adult ; China/epidemiology ; Double-Blind Method ; Embryo Transfer/methods ; Female ; Fertilization in Vitro/drug effects ; Follow-Up Studies ; Humans ; Infertility, Female/drug therapy ; Infertility, Female/epidemiology ; Medicine, Chinese Traditional/methods ; Oocyte Retrieval ; Ovulation Induction/standards ; Pregnancy ; Pregnancy Rate ; Prognosis
Language	English
Publishing date	2021-06-17
Publishing country	Switzerland
Document type	Journal Article ; Multicenter Study ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
ZDB-ID	2592084-4
ISSN	1664-2392
ISSN	1664-2392
DOI	10.3389/fendo.2021.675997
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Article ; Online: Cell-free decellularized cartilage extracellular matrix scaffolds combined with interleukin 4 promote osteochondral repair through immunomodulatory macrophages: In vitro and in vivo preclinical study.

Tian, Guangzhao / Jiang, Shuangpeng / Li, Junqi / Wei, Fu / Li, Xu / Ding, Yi / Yang, Zhen / Sun, Zhiqiang / Zha, Kangkang / Wang, FuXin / Huang, Bo / Peng, Liqing / Wang, Qiuming / Tian, Zhuang / Yang, Xi / Wang, Zhigang / Guo, Quanyi / Guo, Weimin / Liu, Shuyun

Acta biomaterialia

2021 Volume 127, Page(s) 131–145

Abstract: Cartilage regeneration is a complex physiological process. Synovial macrophages play a critical immunomodulatory role in the acute inflammatory response surrounding joint injury. Due to the contrasting differences and heterogeneity of macrophage, the ... ...

Abstract	Cartilage regeneration is a complex physiological process. Synovial macrophages play a critical immunomodulatory role in the acute inflammatory response surrounding joint injury. Due to the contrasting differences and heterogeneity of macrophage, the phenotype of macrophages are the key determinants of the healing response after cartilage injury. Biomaterials derived from extracellular matrix have been used for the repair and reconstruction of a variety of tissues by modulating the host macrophage response. However, the immunomodulatory effect of decellularized cartilage extracellular matrix (ECM) on macrophages has not been elucidated. It is necessary to clarify the immunomodulatory properties of decellularized cartilage matrix (DCM) to guide the design of cartilage regeneration materials. Here, we prepared porcine articular cartilage derived DCM and determined the response of mouse bone marrow-derived macrophages (BMDMs) to the pepsin-solubilized DCM (PDCM) in vitro. Macrophages activated by the PDCM could promote bone marrow-derived mesenchymal stem cells (BMSCs) invasion, migration, proliferation, and chondrogenic differentiation. Then, we verified that early optimization of the immunomodulatory effects of the cell-free DCM scaffold using IL-4 in vivo could achieve good cartilage regeneration in a rat knee osteochondral defect model. Therefore, this decellularized cartilage ECM scaffold combined with accurate and active immunomodulatory strategies provides a new approach for the development of cartilage regeneration materials. STATEMENT OF SIGNIFICANCE: This work reports a decellularized cartilage extracellular matrix (DCM) scaffold combined with an accurate and active immunomodulatory strategy to improve cartilage regeneration. Our findings demonstrated that the pepsin-solubilized DCM (PDCM) activated bone marrow-derived macrophages to polarize to a constructive macrophage phenotype. These polarized macrophages promoted bone marrow-derived mesenchymal stem cell invasion, migration, proliferation, and chondrogenic differentiation. DCM scaffolds combined with early-stage intra-articular injection of IL-4 created a wound-healing microenvironment and improved cartilage regeneration in a rat knee osteochondral defect model.
MeSH term(s)	Animals ; Cartilage, Articular ; Chondrogenesis ; Extracellular Matrix ; Interleukin-4 ; Macrophages ; Mice ; Rats ; Swine ; Tissue Engineering ; Tissue Scaffolds
Chemical Substances	Interleukin-4 (207137-56-2)
Language	English
Publishing date	2021-03-31
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2173841-5
ISSN	1878-7568 ; 1742-7061
ISSN (online)	1878-7568
ISSN	1742-7061
DOI	10.1016/j.actbio.2021.03.054
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Article ; Online: iTRAQ-based proteomic analysis of defence responses triggered by the necrotrophic pathogen Rhizoctonia solani in cotton.

Zhang, Min / Cheng, Shou-Ting / Wang, Hai-Yun / Wu, Jia-He / Luo, Yuan-Ming / Wang, Qian / Wang, Fu-Xin / Xia, Gui-Xian

Journal of proteomics

2017 Volume 152, Page(s) 226–235

Abstract: The soil-borne necrotrophic pathogen fungus Rhizoctonia solani is destructive, causing disease in various important crops. To date, little is known about the host defence mechanism in response to invasion of R. solani. Here, an iTRAQ-based proteomic ... ...

Abstract	The soil-borne necrotrophic pathogen fungus Rhizoctonia solani is destructive, causing disease in various important crops. To date, little is known about the host defence mechanism in response to invasion of R. solani. Here, an iTRAQ-based proteomic analysis was employed to investigate pathogen-responsive proteins in the disease tolerant/resistant cotton cultivar CRI35. A total of 174 differentially accumulated proteins (DAPs) were identified after inoculation of cotton plants with R. solani. Functional categorization analysis indicated that these DAPs can be divided into 12 subclasses. Notably, a large portion of DAPs are known to function in reactive oxygen species (ROS) metabolism and the expression of several histone-modifying and DNA methylating proteins were significantly induced upon challenge with the fungus, indicating that the redox homeostasis and epigenetic regulation are important for cotton defence against the pathogen. Additionally, the expression of proteins involved in phenylpropanoid biosynthesis was markedly changed in response to pathogen invasion, which may reflect a particular contribution of secondary metabolism in protection against the fungal attack in cotton. Together, our results indicate that the defence response of cotton plants to R. solani infection is active and multifaceted and involves the induction of proteins from various innate immunity-related pathways. Significance: Cotton damping-off is a destructive disease caused by the necrotrophic fungus Rhizoctonia solani. To date, the host defence mechanism involved in the disease protection remains largely unknown. Here, we reported the first proteomic analysis on cotton immune responses against R. solani infection. Employing iTRAQ technique, we obtained a total of 174 differentially accumulated proteins (DAPs) that can be classified into 12 functional groups. Further analysis indicated that ROS homeostasis, epigenetic regulation and phenylpropanoid biosynthesis were tightly associated with the innate immune responses against R. solani infection in cotton. The obtained data provide not only important information for understanding the molecular mechanism involved in plant-R. solani interaction but also application clues for genetic breeding of crops with improved R. solani resistance.
Language	English
Publishing date	2017-01-30
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2400835-7
ISSN	1876-7737 ; 1874-3919
ISSN (online)	1876-7737
ISSN	1874-3919
DOI	10.1016/j.jprot.2016.11.011
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Article ; Online: The Cotton Apoplastic Protein CRR1 Stabilizes Chitinase 28 to Facilitate Defense against the Fungal Pathogen

Han, Li-Bo / Li, Yuan-Bao / Wang, Fu-Xin / Wang, Wen-Yan / Liu, Jun / Wu, Jia-He / Zhong, Nai-Qin / Wu, Shen-Jie / Jiao, Gai-Li / Wang, Hai-Yun / Xia, Gui-Xian

The Plant cell

2019 Volume 31, Issue 2, Page(s) 520–536

Abstract: The apoplast serves as the first battlefield between the plant hosts and invading microbes; therefore, work on plant-pathogen interactions has increasingly focused on apoplastic immunity. In this study, we identified three proteins in the apoplast of ... ...

Abstract	The apoplast serves as the first battlefield between the plant hosts and invading microbes; therefore, work on plant-pathogen interactions has increasingly focused on apoplastic immunity. In this study, we identified three proteins in the apoplast of cotton (
MeSH term(s)	Chitinases/genetics ; Chitinases/metabolism ; Disease Resistance/genetics ; Gene Expression Regulation, Plant/genetics ; Gene Expression Regulation, Plant/physiology ; Gossypium/genetics ; Gossypium/metabolism ; Gossypium/microbiology ; Plant Diseases/genetics ; Plant Diseases/microbiology ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Verticillium/pathogenicity
Chemical Substances	Plant Proteins ; Chitinases (EC 3.2.1.14)
Language	English
Publishing date	2019-01-16
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	623171-8
ISSN	1532-298X ; 1040-4651
ISSN (online)	1532-298X
ISSN	1040-4651
DOI	10.1105/tpc.18.00390
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Article: The role of autophagy during development of the oomycete pathogen Phytophthora infestans.

Luo, Qian / Wang, Fu-Xin / Zhong, Nai-Qin / Wang, Hai-Yun / Xia, Gui-Xian

Journal of genetics and genomics = Yi chuan xue bao

2014 Volume 41, Issue 4, Page(s) 225–228

MeSH term(s)	Autophagy ; Oomycetes/parasitology ; Phytophthora infestans/cytology ; Phytophthora infestans/genetics ; Phytophthora infestans/physiology
Language	English
Publishing date	2014-04-20
Publishing country	China
Document type	Letter ; Research Support, Non-U.S. Gov't
ZDB-ID	2374568-X
ISSN	1873-5533 ; 1673-8527
ISSN (online)	1873-5533
ISSN	1673-8527
DOI	10.1016/j.jgg.2014.03.004
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Article ; Online: iTRAQ-based proteomics analysis of autophagy-mediated immune responses against the vascular fungal pathogen Verticillium dahliae in Arabidopsis.

Wang, Fu-Xin / Luo, Yuan-Ming / Ye, Zi-Qin / Cao, Xue / Liang, Jing-Nan / Wang, Qian / Wu, Yao / Wu, Jia-He / Wang, Hai-Yun / Zhang, Min / Cheng, Huan-Qing / Xia, Gui-Xian

Autophagy

2018 Volume 14, Issue 4, Page(s) 598–618

Abstract: The mechanisms underlying the functional link between autophagy and plant innate immunity remain largely unknown. In this study, we investigated the autophagy-mediated plant defense responses against Verticillium dahliae (V. dahliae) infection by ... ...

Abstract	The mechanisms underlying the functional link between autophagy and plant innate immunity remain largely unknown. In this study, we investigated the autophagy-mediated plant defense responses against Verticillium dahliae (V. dahliae) infection by comparative proteomics and cellular analyses. An assessment of the autophagy activity and disease development showed that autophagic processes were tightly related to the tolerance of Arabidopsis plant to Verticillium wilt. An isobaric tags for relative and absolute quantification (iTRAQ)-based proteomics analysis was performed, and we identified a total of 780 differentially accumulated proteins (DAPs) between wild-type and mutant atg10-1 Arabidopsis plants upon V. dahliae infection, of which, 193 ATG8-family-interacting proteins were identified in silico and their associations with autophagy were verified for several selected proteins. Three important aspects of autophagy-mediated defense against V. dahliae infection were revealed: 1) autophagy is required for the activation of upstream defense responses; 2) autophagy-mediated mitochondrial degradation (mitophagy) occurs and is an important player in the defense process; and 3) autophagy promotes the transdifferentiation of perivascular cells and the formation of xylem hyperplasia, which are crucial for protection against this vascular disease. Together, our results provide several novel insights for understanding the functional association between autophagy and plant immune responses.
MeSH term(s)	Arabidopsis/immunology ; Arabidopsis/microbiology ; Autophagy/immunology ; Gene Expression Regulation, Plant/immunology ; Plant Diseases/microbiology ; Plant Proteins/metabolism ; Proteomics/methods ; Verticillium/metabolism
Chemical Substances	Plant Proteins
Language	English
Publishing date	2018-02-21
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2454135-7
ISSN	1554-8635 ; 1554-8627
ISSN (online)	1554-8635
ISSN	1554-8627
DOI	10.1080/15548627.2017.1423438
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Article: [Cloning and functional analysis of chitinase gene GbCHI from sea-island cotton (Gossypium barbadense)].

Ma, Yin-Ping / Wang, Fu-Xin / Yang, Chun-Lin / Shen, Fa-Fu / Xia, Gui-Xian

Yi chuan = Hereditas

2012 Volume 34, Issue 2, Page(s) 240–247

Abstract: Chitinase is one of the important pathogenesis-related (PR) proteins in plants. By comparative proteomics study, a novel pathogen-responsive chitinase (known as GbCHI) has been identified from sea-island cotton (Gossypium barbadense). The GbCHI cDNA was ... ...

Abstract	Chitinase is one of the important pathogenesis-related (PR) proteins in plants. By comparative proteomics study, a novel pathogen-responsive chitinase (known as GbCHI) has been identified from sea-island cotton (Gossypium barbadense). The GbCHI cDNA was cloned from wilt-resistant sea-island cotton and the anti-fungal activity of the gene product was investigated. qRT-PCR analysis indicated that GbCHI was expressed constitutively in root, stem, leaf, flower, and ovule of cotton plant, and the expression could be induced by Verticillium dahliae and plant hormone SA, ACC, and JA. Subcellular localization analysis using GFP-tagged proteins showed that GbCHI-GFP fusion proteins were targeted mainly to the plasma membrane. Anti-fungal assay demonstrated that GbCHI could inhibit spore germination and hyphae growth of V. dahliae significantly. These results provide important information for understanding the cellular function of GbCHI and for exploring the application potential of this gene in molecular breeding of wilt-tolerant cotton plants.
MeSH term(s)	Amino Acid Sequence ; Antifungal Agents/pharmacology ; Chitinases/chemistry ; Chitinases/genetics ; Chitinases/pharmacology ; Cloning, Molecular ; Gossypium/genetics ; Molecular Sequence Data ; Proteomics ; Verticillium/drug effects
Chemical Substances	Antifungal Agents ; Chitinases (EC 3.2.1.14)
Language	Chinese
Publishing date	2012-01-24
Publishing country	China
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	0253-9772
ISSN	0253-9772
DOI	10.3724/sp.j.1005.2012.00240
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