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Article: It is the Frequency that Matters: Effects of Electromagnetic Fields on the Release and Content of Extracellular Vesicles.

Wang, Yihua / Worrell, Gregory A / Wang, Hai-Long

bioRxiv : the preprint server for biology

2023

Abstract: Extracellular vesicles (EVs) are small membrane-bound structures that originate from various cell types and carry molecular cargo to influence the behavior of recipient cells. The use of EVs as biomarkers and delivery vehicles for diagnosis and treatment ...

Abstract	Extracellular vesicles (EVs) are small membrane-bound structures that originate from various cell types and carry molecular cargo to influence the behavior of recipient cells. The use of EVs as biomarkers and delivery vehicles for diagnosis and treatment in a wide range of human disease is a rapidly growing field of research and clinical practice. Four years ago, we postulated the hypothesis that electromagnetic fields (EMF) will influence the release and content of EVs (1). Since then, we have optimized several technical aspects of our experimental setup. We used a bioreactor system that allows cells to grow in a three-dimensional environment mimicking
Language	English
Publishing date	2023-08-11
Publishing country	United States
Document type	Preprint
DOI	10.1101/2023.08.08.552505
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Article ; Online: The emergence of inter-clade hybrid SARS-CoV-2 lineages revealed by 2D nucleotide variation mapping

Wang, Hai-Long

bioRxiv

Abstract: I performed whole-genome sequencing on SARS-CoV-2 collected from COVID-19 samples at Mayo Clinic Rochester in mid-April, 2020, generated 85 consensus genome sequences and compared them to other genome sequences collected worldwide. I proposed a novel ... ...

Abstract	I performed whole-genome sequencing on SARS-CoV-2 collected from COVID-19 samples at Mayo Clinic Rochester in mid-April, 2020, generated 85 consensus genome sequences and compared them to other genome sequences collected worldwide. I proposed a novel illustrating method using a 2D map to display populations of co-occurring nucleotide variants for intra- and inter- viral clades. This method is highly advantageous for the new era of big-data when high-throughput sequencing is becoming readily available. Using this method, I revealed the emergence of inter-clade hybrid SARS-CoV-2 lineages that are potentially caused by homologous genetic recombination.
Keywords	covid19
Language	English
Publishing date	2020-10-14
Publisher	Cold Spring Harbor Laboratory
Document type	Article ; Online
DOI	10.1101/2020.10.13.338038
Database	COVID19

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Article ; Online: The emergence of inter-clade hybrid SARS-CoV-2 lineages revealed by 2D nucleotide variation mapping

Wang, Hai-Long

bioRxiv

Abstract	I performed whole-genome sequencing on SARS-CoV-2 collected from COVID-19 samples at Mayo Clinic Rochester in mid-April, 2020, generated 85 consensus genome sequences and compared them to other genome sequences collected worldwide. I proposed a novel illustrating method using a 2D map to display populations of co-occurring nucleotide variants for intra- and inter-viral clades. This method is highly advantageous for the new era of “big-data” when high-throughput sequencing is becoming readily available. Using this method, I revealed the emergence of inter-clade hybrid SARS-CoV-2 lineages that are potentially caused by homologous genetic recombination.
Keywords	covid19
Publisher	BioRxiv; WHO
Document type	Article ; Online
DOI	10.1101/2020.10.13.338038
Database	COVID19

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Article: The many roles of cathepsins in restenosis.

Wang, Hai Long / Narisawa, Megumi / Wu, Pan / Meng, Xiangkun / Cheng, Xian Wu

Heliyon

2024 Volume 10, Issue 3, Page(s) e24720

Abstract: Drug-eluting stents (DES) and dual antiplatelet regimens have significantly improved the clinical management of ischemic heart disease; however, the drugs loaded with DES in clinical practice are mostly paclitaxel or rapamycin derivatives, which target ... ...

Abstract	Drug-eluting stents (DES) and dual antiplatelet regimens have significantly improved the clinical management of ischemic heart disease; however, the drugs loaded with DES in clinical practice are mostly paclitaxel or rapamycin derivatives, which target symptoms of post implantation proliferation and inflammation, leading to delayed re-endothelialization and neo-atherosclerosis. Along with the treatments already in place, there is a need for novel strategies to lessen the negative clinical outcomes of DES delays as well as a need for greater understanding of their pathobiological mechanisms. This review concentrates on the function of cathepsins (Cats) in the inflammatory response and granulation tissue formation that follow Cat-induced damage to the vasculature scaffold, as well as the functions of Cats in intimal hyperplasia, which is characterized by the migration and proliferation of smooth muscle cells, and endothelial denudation, re-endothelialization, and/or neo-endothelialization. Additionally, Cats can alter essential neointima formation and immune response inside scaffolds, and if Cats are properly controlled in vivo, they may improve scaffold biocompatibility. This unique profile of functions could lead to an original concept for a cathepsin-based coronary intervention treatment as an adjunct to stent placement.
Language	English
Publishing date	2024-02-01
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2024.e24720
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Inhibiting MMP13 Attenuates Deep Vein Thrombosis in a Mouse Model by Reducing the Expression of Pdpn.

Luo, Ji / Zhou, Jin / Luo, Jing-Zeng / Wang, Hai-Long / Zhao, Xue-Ling / Zhou, Ru-Dan

Current medical science

2024 Volume 44, Issue 2, Page(s) 369–379

Abstract: Objective: Matrix metalloproteinase 13 (MMP13) is an extracellular matrix protease that affects the progression of atherosclerotic plaques and arterial thrombi by degrading collagens, modifying protein structures and regulating inflammatory responses, ... ...

Abstract	Objective: Matrix metalloproteinase 13 (MMP13) is an extracellular matrix protease that affects the progression of atherosclerotic plaques and arterial thrombi by degrading collagens, modifying protein structures and regulating inflammatory responses, but its role in deep vein thrombosis (DVT) has not been determined. The purpose of this study was to investigate the potential effects of MMP13 and MMP13-related genes on the formation of DVT. Methods: We altered the expression level of MMP13 in vivo and conducted a transcriptome study to examine the expression and relationship between MMP13 and MMP13-related genes in a mouse model of DVT. After screening genes possibly related to MMP13 in DVT mice, the expression levels of candidate genes in human umbilical vein endothelial cells (HUVECs) and the venous wall were evaluated. The effect of MMP13 on platelet aggregation in HUVECs was investigated in vitro. Results: Among the differentially expressed genes, interleukin 1 beta, podoplanin (Pdpn), and factor VIII von Willebrand factor (F8VWF) were selected for analysis in mice. When MMP13 was inhibited, the expression level of PDPN decreased significantly in vitro. In HUVECs, overexpression of MMP13 led to an increase in the expression level of PDPN and induced platelet aggregation, while transfection of PDPN-siRNA weakened the ability of MMP13 to increase platelet aggregation. Conclusions: Inhibiting the expression of MMP13 could reduce the burden of DVT in mice. The mechanism involves downregulating the expression of Pdpn through MMP13, which could provide a novel gene target for DVT diagnosis and treatment.
MeSH term(s)	Animals ; Humans ; Mice ; Disease Models, Animal ; Human Umbilical Vein Endothelial Cells/metabolism ; Matrix Metalloproteinase 13/genetics ; Platelet Aggregation ; Venous Thrombosis/genetics
Chemical Substances	Matrix Metalloproteinase 13 (EC 3.4.24.-) ; MMP13 protein, human (EC 3.4.24.-) ; Mmp13 protein, mouse (EC 3.4.24.-) ; PDPN protein, human
Language	English
Publishing date	2024-04-15
Publishing country	China
Document type	Journal Article
ZDB-ID	2931065-9
ISSN	2523-899X ; 2096-5230
ISSN (online)	2523-899X
ISSN	2096-5230
DOI	10.1007/s11596-024-2862-6
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Article: Research on vibration effect of tunnel blasting based on an improved Hilbert–Huang transform

Zhao, Yan / Shan, Ren liang / Wang, Hai long

Environmental earth sciences. 2021 Mar., v. 80, no. 5

2021

Abstract: Through a tunnel-blasting project, the effect of tunnel-blasting vibration has been analyzed from the perspective of vibration energy transfer. The non-linear regression method was used to obtain the prediction equation for blast vibration velocity based ...

Abstract	Through a tunnel-blasting project, the effect of tunnel-blasting vibration has been analyzed from the perspective of vibration energy transfer. The non-linear regression method was used to obtain the prediction equation for blast vibration velocity based on the field blast vibration data. Then, the maximum charge per delay of the blasting construction of the tunnel was obtained through the formula inversion. Based on the traditional Hilbert transform, a novel Hilbert–Huang transform (HHT) analysis method considering Complete Ensemble Empirical Mode Decomposition with Adaptive Noise decomposition (CEEMDAN) and wavelet packet threshold de-noising method has been proposed, the feasibility of which was verified using the field blast vibration signals. It has been proved that the improved HHT analysis method can be used to analyze the influence of the different blasting vibration parameters on the regular distribution of vibration energy. In addition, the dimensional analysis method was used to establish the blasting vibration energy prediction model. The results of this research show that the improved HHT analysis method can solve the problem of modal aliasing caused by the traditional decomposition method, and can obtain the purified main modal components, which improves the adaptability of HHT analysis. In addition, with the increase of the distance between blast area and the maximum charge per delay, the high-frequency energy of the blasting signal gradually weakens, while the dominant energy frequency band diverts to the low-frequency band. The methods and conclusions of this research can provide a certain reference for the controlled blasting construction in similar cases.
Keywords	energy transfer ; equations ; models ; prediction ; regression analysis ; vibration ; wavelet
Language	English
Dates of publication	2021-03
Size	p. 206.
Publishing place	Springer Berlin Heidelberg
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	2493699-6
ISSN	1866-6299 ; 1866-6280
ISSN (online)	1866-6299
ISSN	1866-6280
DOI	10.1007/s12665-021-09506-9
Database	NAL-Catalogue (AGRICOLA)

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Article: Establishment of a prognosis predictive model for liver cancer based on expression of genes involved in the ubiquitin-proteasome pathway.

Li, Hua / Ma, Yi-Po / Wang, Hai-Long / Tian, Cai-Juan / Guo, Yi-Xian / Zhang, Hong-Bo / Liu, Xiao-Min / Liu, Peng-Fei

World journal of clinical oncology

2024 Volume 15, Issue 3, Page(s) 434–446

Abstract: Background: The ubiquitin-proteasome pathway (UPP) has been proven to play important roles in cancer.: Aim: To investigate the prognostic significance of genes involved in the UPP and develop a predictive model for liver cancer based on the ... ...

Abstract	Background: The ubiquitin-proteasome pathway (UPP) has been proven to play important roles in cancer. Aim: To investigate the prognostic significance of genes involved in the UPP and develop a predictive model for liver cancer based on the expression of these genes. Methods: In this study, UPP-related E1, E2, E3, deubiquitylating enzyme, and proteasome gene sets were obtained from the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, aiming to screen the prognostic genes using univariate and multivariate regression analysis and develop a prognosis predictive model based on the Cancer Genome Atlas liver cancer cases. Results: Five genes (including autophagy related 10, proteasome 20S subunit alpha 8, proteasome 20S subunit beta 2, ubiquitin specific peptidase 17 like family member 2, and ubiquitin specific peptidase 8) were proven significantly correlated with prognosis and used to develop a prognosis predictive model for liver cancer. Among training, validation, and Gene Expression Omnibus sets, the overall survival differed significantly between the high-risk and low-risk groups. The expression of the five genes was significantly associated with immunocyte infiltration, tumor stage, and postoperative recurrence. A total of 111 differentially expressed genes (DEGs) were identified between the high-risk and low-risk groups and they were enriched in 20 and 5 gene ontology and KEGG pathways. Cell division cycle 20, Kelch repeat and BTB domain containing 11 Conclusion: We have constructed a prognosis predictive model in patients with liver cancer, which contains five genes that associate with immunocyte infiltration, tumor stage, and postoperative recurrence.
Language	English
Publishing date	2024-03-27
Publishing country	United States
Document type	Journal Article
ZDB-ID	2587357-X
ISSN	2218-4333
ISSN	2218-4333
DOI	10.5306/wjco.v15.i3.434
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Article ; Online: Nuclear matrix protein 22 in bladder cancer.

Cheng, Kun / Wan, Shun / Chen, Si-Yu / Yang, Jian-Wei / Wang, Hai-Long / Xu, Chang-Hong / Qiao, Si-Hang / Yang, Li

Clinica chimica acta; international journal of clinical chemistry

2024 Volume 560, Page(s) 119718

Abstract: Bladder cancer (BC) is ranked as the ninth most common malignancy worldwide, with approximately 570,000 new cases reported annually and over 200,000 deaths. Cystoscopy remains the gold standard for the diagnosis of BC, however, its invasiveness, cost, ... ...

Abstract	Bladder cancer (BC) is ranked as the ninth most common malignancy worldwide, with approximately 570,000 new cases reported annually and over 200,000 deaths. Cystoscopy remains the gold standard for the diagnosis of BC, however, its invasiveness, cost, and discomfort have driven the demand for the development of non-invasive, cost-effective alternatives. Nuclear matrix protein 22 (NMP22) is a promising non-invasive diagnostic tool, having received FDA approval. Traditional methods for detecting NMP22 require a laboratory environment equipped with specialized equipment and trained personnel, thus, the development of NMP22 detection devices holds substantial potential for application. In this review, we evaluate the NMP22 sensors developed over the past decade, including electrochemical, colorimetric, and fluorescence biosensors. These sensors have enhanced detection sensitivity and overcome the limitations of existing diagnostic methods. However, many emerging devices exhibit deficiencies that limit their potential clinical use, therefore, we propose how sensor design can be optimized to enhance the likelihood of clinical translation and discuss the future applications of NMP22 as a legacy biomarker, providing insights for the design of new sensors.
Language	English
Publishing date	2024-05-07
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	80228-1
ISSN	1873-3492 ; 0009-8981
ISSN (online)	1873-3492
ISSN	0009-8981
DOI	10.1016/j.cca.2024.119718
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Article ; Online: ANKRD49 promotes the metastasis of NSCLC via activating JNK-ATF2/c-Jun-MMP-2/9 axis.

Sun, Jia / Hu, Jin-Rui / Liu, Chao-Feng / Li, Yuan / Wang, Wei / Fu, Rong / Guo, Min / Wang, Hai-Long / Pang, Min

BMC cancer

2023 Volume 23, Issue 1, Page(s) 1108

Abstract: Background: Ankyrin repeat domain 49 (ANKRD49) has been found to be highly expressed in multiple cancer including lung adenocarcinoma (LUAD) and lung squamous carcinoma (LUSC). However, the function of ANKRD49 in the pathogenesis of NSCLC still remains ... ...

Abstract	Background: Ankyrin repeat domain 49 (ANKRD49) has been found to be highly expressed in multiple cancer including lung adenocarcinoma (LUAD) and lung squamous carcinoma (LUSC). However, the function of ANKRD49 in the pathogenesis of NSCLC still remains elusive. Previously, ANKRD49 has been demonstrated to promote the invasion and metastasis of A549 cells, a LUAD cell line, via activating the p38-ATF-2-MMP2/MMP9 pathways. Considering the heterogeneity of tumor cells, the function and mechanism of ANKRD49 in NSCLC need more NSCLC-originated cells to clarify. Methods: Real-time qPCR was employed to test ANKRD49 expression levels in nine pairs of fresh NSCLC tissues and the corresponding adjacent normal tissues. The function of ANKRD49 was investigated using overexpression and RNA interference assays in lung adenocarcinoma cell line (NCI-H1299) and lung squamous carcinoma cell line (NCI-H1703) through gelatin zymography, cell counting kit-8, colony formation, wound healing, migration and invasion assays mmunoprecipitation was performed to in vitro. Immunoprecipitation was performed to test the interaction of c-Jun and ATF2. Chromatin immunoprecipitation was conducted to assess the transcriptional regulation of ATF2/c-Jun on MMP-2/9. Moreover, the tumorigenicity of ANKRD49 was evaluated in nude mice models and the involved signal molecular was also measured by immunohistochemical method. Results: We found that the levels of ANKRD49 in cancerous tissues were higher than those in adjacent normal tissues. in vitro assay showed that ANKRD49 promoted the migration and invasion of NCI-H1299 and NCI-H1703 cells via enhancing the levels of MMP-2 and MMP-9. Furthermore, ANKRD49 elevated phosphorylation of JNK and then activated c-Jun and ATF2 which interact in nucleus to promote the binding of ATF2:c-Jun with the promoter MMP-2 or MMP-9. In vivo assay showed that ANKRD49 promoted lung metastasis of injected-NSCLC cells and the high metastatic rate was positively correlated with the high expression of ANKRD49, MMP-2, MMP-9, p-JNK, p-c-Jun and p-ATF2. Conclusion: The present study indicated that ANKRD49 accelerated the invasion and metastasis of NSCLC cells via JNK-mediated transcription activation of c-Jun and ATF2 which regulated the expression of MMP-2/MMP-9. The molecular mechanisms of ANKRD49's function is different from those found in A549 cells. The current study is a supplement and improvement to the previous research.
MeSH term(s)	Animals ; Mice ; Matrix Metalloproteinase 2/metabolism ; Matrix Metalloproteinase 9/metabolism ; Mice, Nude ; Cell Proliferation/genetics ; Cell Line, Tumor ; Carcinoma, Non-Small-Cell Lung/pathology ; Lung Neoplasms/pathology ; Carcinoma, Squamous Cell ; Adenocarcinoma of Lung
Chemical Substances	Matrix Metalloproteinase 2 (EC 3.4.24.24) ; Matrix Metalloproteinase 9 (EC 3.4.24.35)
Language	English
Publishing date	2023-11-14
Publishing country	England
Document type	Journal Article
ZDB-ID	2041352-X
ISSN	1471-2407 ; 1471-2407
ISSN (online)	1471-2407
ISSN	1471-2407
DOI	10.1186/s12885-023-11612-9
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Article ; Online: Intranasal immunisation with recombinant Toxoplasma gondii uridine phosphorylase confers resistance against acute toxoplasmosis in mice.

Yin, Li-Tian / Ren, Ying-Jie / You, Yu-Jie / Yang, Yong / Wang, Zhi-Xin / Wang, Hai-Long

Parasite (Paris, France)

2023 Volume 30, Page(s) 46

Abstract: Toxoplasmosis is caused by Toxoplasma gondii, which infects all warm-blooded animals, including humans. Currently, control measures for T. gondii infection are insufficient due to the lack of effective medications or vaccines. In this paper, recombinant ... ...

Title translation	L’immunisation intranasale avec l’uridine phosphorylase recombinante de Toxoplasma gondii confère une résistance contre la toxoplasmose aiguë chez la souris.
Abstract	Toxoplasmosis is caused by Toxoplasma gondii, which infects all warm-blooded animals, including humans. Currently, control measures for T. gondii infection are insufficient due to the lack of effective medications or vaccines. In this paper, recombinant T. gondii uridine phosphorylase (rTgUPase) was expressed in Escherichia coli and purified via Ni
MeSH term(s)	Humans ; Female ; Animals ; Mice ; Toxoplasma/genetics ; Uridine Phosphorylase/genetics ; Protozoan Proteins/genetics ; Cytokines ; Toxoplasmosis ; Immunization ; Immunoglobulin G ; Protozoan Vaccines ; Mice, Inbred BALB C ; Antibodies, Protozoan ; Toxoplasmosis, Animal/prevention & control
Chemical Substances	Uridine Phosphorylase (EC 2.4.2.3) ; Protozoan Proteins ; Cytokines ; Immunoglobulin G ; Protozoan Vaccines ; Antibodies, Protozoan
Language	English
Publishing date	2023-11-02
Publishing country	France
Document type	Journal Article
ZDB-ID	1187629-3
ISSN	1776-1042 ; 1252-607X
ISSN (online)	1776-1042
ISSN	1252-607X
DOI	10.1051/parasite/2023047
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