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  1. Article ; Online: Combination of MAR and intron increase transgene expression of episomal vectors in CHO cells.

    Wang, Xiao-Yin / Zhang, Wei-Li / Zhang, Xi / Fu, Yu-Shun / Wang, Hao-Min / Sun, Qiu-Li / Li, Qin / Jia, Yan-Long / Zhang, Jun-He / Wang, Tian-Yun

    Biotechnology journal

    2023  Volume 18, Issue 12, Page(s) e2200643

    Abstract: Previous work has shown that the EF-1α promoter of episomal vectors maintains high-level transgene expression in stably transfected Chinese hamster ovary (CHO) cells. However, the transgene expression levels need to be further increased. Here, we first ... ...

    Abstract Previous work has shown that the EF-1α promoter of episomal vectors maintains high-level transgene expression in stably transfected Chinese hamster ovary (CHO) cells. However, the transgene expression levels need to be further increased. Here, we first incorporated matrix attachment regions (MARs), ubiquitous chromatin opening element (UCOE), stabilizing anti repressor elements 40 (STAR 40) elements into episomal vector at different sites and orientations, and systemically assessed their effects on transgene expression in transfected CHO-K1 cells. Results showed that enhanced green fluorescent protein (eGFP) expression levels increased remarkably when MAR X-29 was inserted upstream of the promoter, followed by the insertion of MAR1 downstream of the poly A, and the orientation had no significant effect. Moreover, MAR X-29 combined with human cytomegalovirus intron (hCMVI) yielded the highest transgene expression levels (4.52-fold). Transgene expression levels were not exclusively dependent on transgene copy numbers and were not related to the mRNA expression level. In addition, vector with MAR X-29+hCMVI can induce herpes simplex virus thymidine kinase (HSV-TK) protein expression, and the HSV-TK protein showed a cell-killing effect and an obvious bystander effect on HCT116 cells. In conclusion, the combination of MAR X-29 and hCMV intron can achieve high efficiency transgene expression mediated by episomal vectors in CHO-K1 cells.
    MeSH term(s) Cricetinae ; Animals ; Humans ; Cricetulus ; Transfection ; CHO Cells ; Introns/genetics ; Transgenes/genetics ; Matrix Attachment Regions/genetics ; Genetic Vectors/genetics
    Language English
    Publishing date 2023-08-21
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2221885-3
    ISSN 1860-7314 ; 1860-6768
    ISSN (online) 1860-7314
    ISSN 1860-6768
    DOI 10.1002/biot.202200643
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  2. Article ; Online: Hilbert-Huang Transformation Based Analyses of FP1, FP2, and Fz Electroencephalogram Signals in Alcoholism.

    Lin, Chin-Feng / Su, Jiun-Yi / Wang, Hao-Min

    Journal of medical systems

    2015  Volume 39, Issue 9, Page(s) 83

    Abstract: Chronic alcoholism may damage the central nervous system, causing imbalance in the excitation-inhibition homeostasis in the cortex, which may lead to hyper-arousal of the central nervous system, and impairments in cognitive function. In this paper, we ... ...

    Abstract Chronic alcoholism may damage the central nervous system, causing imbalance in the excitation-inhibition homeostasis in the cortex, which may lead to hyper-arousal of the central nervous system, and impairments in cognitive function. In this paper, we use the Hilbert-Huang transformation (HHT) method to analyze the electroencephalogram (EEG) signals from control and alcoholic observers who watched two different pictures. We examined the intrinsic mode function (IMF) based energy distribution features of FP1, FP2, and Fz EEG signals in the time and frequency domains for alcoholics. The HHT-based characteristics of the IMFs, the instantaneous frequencies, and the time-frequency-energy distributions of the IMFs of the clinical FP1, FP2, and Fz EEG signals recorded from normal and alcoholic observers who watched two different pictures were analyzed. We observed that the number of peak amplitudes of the alcoholic subjects is larger than that of the control. In addition, the Pearson correlation coefficients of the IMFs, and the energy-IMF distributions of the clinical FP1, FP2, and Fz EEG signals recorded from normal and alcoholic observers were analyzed. The analysis results show that the energy ratios of IMF4, IMF5, and IMF7 waves of the normal observers to the refereed total energy were larger than 10 %, respectively. In addition, the energy ratios of IMF3, IMF4, and IMF5 waves of the alcoholic observers to the refereed total energy were larger than 10 %. The FP1 and FP2 waves of the normal observers, the FP1 and FP2 waves of the alcoholic observers, and the FP1 and Fz waves of the alcoholic observers demonstrated extremely high correlations. On the other hand, the FP1 waves of the normal and alcoholic observers, the FP1 wave of the normal observer and the FP2 wave of the alcoholic observer, the FP1 wave of the normal observer and the Fz wave of the alcoholic observer, the FP2 waves of the normal and alcoholic FP2 observers, and the FP2 wave of the normal observer and the Fz wave of the alcoholic observer demonstrated extremely low correlations. The IMF4 of the FP1 and FP2 signals of the normal observer, and the IMF5 of the FP1 and FP2 signals of the alcoholic observer were correlated. The IMF4 of the FP1 signal of the normal observer and that of the FP2 signal of the alcoholic observer as well as the IMF5 of the FP1 signal of the normal observer and that of the FP2 signal of the alcoholic observer exhibited extremely low correlations. In this manner, our experiment leads to a better understanding of the HHT-based IMFs features of FP1, FP2, and Fz EEG signals in alcoholism. The analysis results show that the energy ratios of the wave of an alcoholic observer to its refereed total energy for IMF4, and IMF5 in the δ band for FP1, FP2, and Fz channels were larger than those of the respective waves of the normal observer. The alcoholic EEG signals constitute more than 1 % of the total energy in the δ wave, and the reaction times were 0_4, 4_8, 8_12, and 12_16 s. For normal EEG signals, more than 1 % of the total energy is distributed in the δ wave, with a reaction time 0 to 4 s. We observed that the alcoholic subject reaction times were slower than those of the normal subjects, and the alcoholic subjects could have experienced a cognitive error. This phenomenon is due to the intoxicated central nervous systems of the alcoholic subjects.
    MeSH term(s) Alcoholism/physiopathology ; Algorithms ; Chronic Disease ; Electroencephalography ; Humans ; Reaction Time ; Signal Processing, Computer-Assisted
    Language English
    Publishing date 2015-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 423488-1
    ISSN 1573-689X ; 0148-5598
    ISSN (online) 1573-689X
    ISSN 0148-5598
    DOI 10.1007/s10916-015-0275-6
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    Zs.A 1426: Show issues Location:
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  3. Article: Post-treatment With Qing-Ying-Tang, a Compound Chinese Medicine Relives Lipopolysaccharide-Induced Cerebral Microcirculation Disturbance in Mice.

    Wang, Hao-Min / Huang, Ping / Li, Quan / Yan, Lu-Lu / Sun, Kai / Yan, Li / Pan, Chun-Shui / Wei, Xiao-Hong / Liu, Yu-Ying / Hu, Bai-He / Wang, Chuan-She / Fan, Jing-Yu / Han, Jing-Yan

    Frontiers in physiology

    2019  Volume 10, Page(s) 1320

    Abstract: ... ...

    Abstract Objective
    Language English
    Publishing date 2019-10-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2564217-0
    ISSN 1664-042X
    ISSN 1664-042X
    DOI 10.3389/fphys.2019.01320
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  4. Article ; Online: Tunable stress and controlled thickness modification in graphene by annealing.

    Ni, Zhen Hua / Wang, Hao Min / Ma, Yun / Kasim, Johnson / Wu, Yi Hong / Shen, Ze Xiang

    ACS nano

    2008  Volume 2, Issue 5, Page(s) 1033–1039

    Abstract: Graphene has many unique properties which make it an attractive material for fundamental study as well as for potential applications. In this paper, we report the first experimental study of process-induced defects and stress in graphene using Raman ... ...

    Abstract Graphene has many unique properties which make it an attractive material for fundamental study as well as for potential applications. In this paper, we report the first experimental study of process-induced defects and stress in graphene using Raman spectroscopy and imaging. While defects lead to the observation of defect-related Raman bands, stress causes shift in phonon frequency. A compressive stress (as high as 2.1 GPa) was induced in graphene by depositing a 5 nm SiO(2) followed by annealing, whereas a tensile stress ( approximately 0.7 GPa) was obtained by depositing a thin silicon capping layer. In the former case, both the magnitude of the compressive stress and number of graphene layers can be controlled or modified by the annealing temperature. As both the stress and thickness affect the physical properties of graphene, this study may open up the possibility of utilizing thickness and stress engineering to improve the performance of graphene-based devices. Local heating techniques may be used to either induce the stress or reduce the thickness selectively.
    MeSH term(s) Compressive Strength ; Crystallization/methods ; Elastic Modulus ; Graphite/chemistry ; Hardness ; Hot Temperature ; Macromolecular Substances/chemistry ; Materials Testing ; Molecular Conformation ; Nanostructures/chemistry ; Nanostructures/ultrastructure ; Nanotechnology/methods ; Particle Size ; Surface Properties ; Tensile Strength
    Chemical Substances Macromolecular Substances ; Graphite (7782-42-5)
    Language English
    Publishing date 2008-05
    Publishing country United States
    Document type Journal Article
    ISSN 1936-086X
    ISSN (online) 1936-086X
    DOI 10.1021/nn800031m
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Book ; Online: Tunable stress and controlled thickness modification in graphene by annealing

    Ni, Zhen Hua / Wang, Hao Min / Ma, Yun / Kasim, Johnson / Wu, Yi Hong / Shen, Ze Xiang

    2008  

    Abstract: Graphene has many unique properties which make it an attractive material for fundamental study as well as for potential applications. In this paper, we report the first experimental study of process-induced defects and stress in graphene using Raman ... ...

    Abstract Graphene has many unique properties which make it an attractive material for fundamental study as well as for potential applications. In this paper, we report the first experimental study of process-induced defects and stress in graphene using Raman spectroscopy and imaging. While defects lead to the observation of defect-related Raman bands, stress causes shift in phonon frequency. A compressive stress (as high as 2.1 GPa) was induced in graphene by depositing a 5 nm SiO2 followed by annealing, whereas a tensile stress (~ 0.7 GPa) was obtained by depositing a thin silicon capping layer. In the former case, both the magnitude of the compressive stress and number of graphene layers can be controlled or modified by the annealing temperature. As both the stress and thickness affect the physical properties of graphene, this study may open up the possibility of utilizing thickness and stress engineering to improve the performance of graphene-based devices. Local heating techniques may be used to either induce the stress or reduce the thickness selectively.

    Comment: 19 pages, 7 figures, accepted by ACS nano
    Keywords Condensed Matter - Materials Science
    Subject code 620
    Publishing date 2008-05-07
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Electro-acupuncture stimulation improves motor disorders in Parkinsonian rats.

    Jia, Jun / Sun, ZuoLi / Li, Bo / Pan, YanLi / Wang, HaoMin / Wang, Xuan / Yu, Fen / Liu, LiMin / Zhang, LiJuan / Wang, XiaoMin

    Behavioural brain research

    2009  Volume 205, Issue 1, Page(s) 214–218

    Abstract: Electro-acupuncture (EA) is believed to be effective for alleviating motor symptoms in patients with Parkinson's disease. In a rat hemiparkinsonian model induced by unilateral transection of the medial forebrain bundle (MFB), the effects of EA ... ...

    Abstract Electro-acupuncture (EA) is believed to be effective for alleviating motor symptoms in patients with Parkinson's disease. In a rat hemiparkinsonian model induced by unilateral transection of the medial forebrain bundle (MFB), the effects of EA stimulation were investigated. EA stimulation at a high frequency (100 Hz) significantly reduced apomorphine-induced rotational behavior. Tyrosine hydroxylase immunohistochemical staining revealed that EA at 100 Hz protected axotomized dopaminergic neurons from degeneration in the substantia nigra (SN). Moreover, high frequency EA reversed the axotomy-induced decrease in substance P content and increase in glutamate decarboxylase-67 (GAD 67) mRNA level in the midbrain; however, it did not affect the axotomy-induced increase in enkephalin content in the globus pallidus. These results suggest that the effects of high frequency EA on motor symptoms of Parkinsonian rats may involve restoration of the homeostasis of dopaminergic transmission in the basal ganglia circuit.
    MeSH term(s) Animals ; Antiparkinson Agents/adverse effects ; Apomorphine/adverse effects ; Brain/drug effects ; Brain/pathology ; Brain/physiopathology ; Disease Models, Animal ; Dopamine/metabolism ; Dyskinesias/pathology ; Dyskinesias/physiopathology ; Dyskinesias/therapy ; Electroacupuncture/methods ; Enkephalins/metabolism ; Glutamate Decarboxylase/metabolism ; Male ; Medial Forebrain Bundle/injuries ; Motor Activity/drug effects ; Motor Activity/physiology ; Neurons/drug effects ; Neurons/pathology ; Neurons/physiology ; Parkinsonian Disorders/pathology ; Parkinsonian Disorders/physiopathology ; Parkinsonian Disorders/therapy ; RNA, Messenger/metabolism ; Random Allocation ; Rats ; Rats, Wistar ; Substance P/metabolism ; Tyrosine 3-Monooxygenase/metabolism
    Chemical Substances Antiparkinson Agents ; Enkephalins ; RNA, Messenger ; Substance P (33507-63-0) ; Tyrosine 3-Monooxygenase (EC 1.14.16.2) ; Glutamate Decarboxylase (EC 4.1.1.15) ; glutamate decarboxylase 1 (EC 4.1.1.15) ; Apomorphine (N21FAR7B4S) ; Dopamine (VTD58H1Z2X)
    Language English
    Publishing date 2009-12-14
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 449927-x
    ISSN 1872-7549 ; 0166-4328
    ISSN (online) 1872-7549
    ISSN 0166-4328
    DOI 10.1016/j.bbr.2009.06.024
    Database MEDical Literature Analysis and Retrieval System OnLINE

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