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Article ; Online: Genetic alterations and allele frequency of BRAF V600E and TERT mutation in papillary thyroid carcinoma with intermediate-to-high recurrence risk: a retrospective study.

Huang, Jiayu / Wang, Jiazhi / Xv, Jingchao / Wang, Jingran / Wang, Guangzhi / Zhao, Yongfu

2024 Volume 24, Issue 1, Page(s) 76

Abstract: The predictive value of allele frequency (AF) of BRAF V600E and TERT mutations in papillary thyroid carcinoma (PTC) remains controversial. We aimed to investigate the AF of BRAF V600E and TERT mutations in intermediate-to-high risk PTC and their ... ...

Abstract	The predictive value of allele frequency (AF) of BRAF V600E and TERT mutations in papillary thyroid carcinoma (PTC) remains controversial. We aimed to investigate the AF of BRAF V600E and TERT mutations in intermediate-to-high risk PTC and their association between tumor invasiveness, prognosis, and other mutations. Probe hybridization capture and high-throughput sequencing were used to quantitatively test 40 gene loci in 94 intermediate-to-high recurrence risk PTC patients, combined with clinical characteristics and follow-up for retrospective analysis. BRAF V600E mutation AF was linked to a increased risk of thyroid capsule penetration, recurrence, and concurrent mutations. Concurrent mutations could lead to a worse prognosis and increased invasiveness. TERT promoter mutation frequently accompanied other mutations and resulted in a poorer prognosis. However, there was no clear association between the TERT mutation AF and tumor invasiveness or recurrence. The sensitivity and specificity of predicting recurrence in intermediate-to-high risk PTC with BRAF V600E mutation AF > 28.2% were 60 and 80%. Although genetic alterations in PTC can differ among different ethnicities, the AF of BRAF V600E and TERT mutations may be similar. The AF of BRAF V600E has the potential to be a novel indicator in predicting PTC invasiveness and prognosis.
MeSH term(s)	Humans ; Thyroid Cancer, Papillary/genetics ; Retrospective Studies ; Proto-Oncogene Proteins B-raf/genetics ; Mutation ; Gene Frequency ; Thyroid Neoplasms/genetics ; Telomerase/genetics
Chemical Substances	Proto-Oncogene Proteins B-raf (EC 2.7.11.1) ; TERT protein, human (EC 2.7.7.49) ; Telomerase (EC 2.7.7.49) ; BRAF protein, human (EC 2.7.11.1)
Language	English
Publishing date	2024-04-12
Publishing country	Italy
Document type	Journal Article
ZDB-ID	2053018-3
ISSN	1591-9528 ; 1591-8890
ISSN (online)	1591-9528
ISSN	1591-8890
DOI	10.1007/s10238-024-01320-4
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Article ; Online: Linking Autophagy to Potential Agronomic Trait Improvement in Crops.

Wang, Jingran / Miao, Shulei / Liu, Yule / Wang, Yan

International journal of molecular sciences

2022 Volume 23, Issue 9

Abstract: Autophagy is an evolutionarily conserved catabolic process in eukaryotic cells, by which the superfluous or damaged cytoplasmic components can be delivered into vacuoles or lysosomes for degradation and recycling. Two decades of autophagy research in ... ...

Abstract	Autophagy is an evolutionarily conserved catabolic process in eukaryotic cells, by which the superfluous or damaged cytoplasmic components can be delivered into vacuoles or lysosomes for degradation and recycling. Two decades of autophagy research in plants uncovers the important roles of autophagy during diverse biological processes, including development, metabolism, and various stress responses. Additionally, molecular machineries contributing to plant autophagy onset and regulation have also gradually come into people's sights. With the advancement of our knowledge of autophagy from model plants, autophagy research has expanded to include crops in recent years, for a better understanding of autophagy engagement in crop biology and its potentials in improving agricultural performance. In this review, we summarize the current research progress of autophagy in crops and discuss the autophagy-related approaches for potential agronomic trait improvement in crop plants.
MeSH term(s)	Autophagy ; Crops, Agricultural/genetics ; Humans
Language	English
Publishing date	2022-04-26
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms23094793
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Article: Enhanced hepatic metabolic perturbation of polystyrene nanoplastics by UV irradiation-induced hydroxyl radical generation.

He, Shiyu / Wang, Jingran / Zhou, Lihong / Mao, Zhen / Zhang, Xiaodan / Cai, Jin / Huang, Peili

Journal of environmental sciences (China)

2023 Volume 142, Page(s) 259–268

Abstract: The environmental behavior of and risks associated with nanoplastics (NPs) have attracted considerable attention. However, compared to pristine NPs, environmental factors such as ultraviolet (UV) irradiation that lead to changes in the toxicity of NPs ... ...

Abstract	The environmental behavior of and risks associated with nanoplastics (NPs) have attracted considerable attention. However, compared to pristine NPs, environmental factors such as ultraviolet (UV) irradiation that lead to changes in the toxicity of NPs have rarely been studied. We evaluated the changes in morphology and physicochemical properties of polystyrene (PS) NPs before and after UV irradiation, and compared their hepatotoxicity in mice. The results showed that UV irradiation caused particle size reduction and increased the carbonyl index (CI) and negative charge on the particle surface. UV-aged PS NPs (aPS NPs) could induce the generation of hydroxyl radicals (·OH), but also further promoted the generation of ·OH in the Fenton reaction system. Hepatic pathological damage was more severe in mice exposed to aPS NPs, accompanied by a large number of vacuoles and hepatocyte balloon-like changes and more marked perturbations in blood glucose and serum lipoprotein, alanine aminotransferase and aspartate aminotransferase levels. In addition, exposure to PS NPs and aPS NPs, especially aPS NPs, triggered oxidative stress and significantly damaged the antioxidant capacity of mice liver. Compared with PS NPs, exposure to aPS NPs increased the number of altered metabolites in hepatic and corresponding metabolic pathways, especially glutathione metabolism. Our research suggests that UV irradiation can disrupt the redox balance in organisms by promoting the production of ·OH, enhancing PS NPs-induced liver damage and metabolic disorders. This study will help us understand the health risks of NPs and to avoid underestimation of the risks of NPs in nature.
MeSH term(s)	Animals ; Mice ; Hydroxyl Radical ; Microplastics/toxicity ; Polystyrenes/toxicity ; Ultraviolet Rays ; Liver ; Nanoparticles/toxicity ; Water Pollutants, Chemical
Chemical Substances	Hydroxyl Radical (3352-57-6) ; Microplastics ; Polystyrenes ; Water Pollutants, Chemical
Language	English
Publishing date	2023-06-30
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1092300-7
ISSN	1878-7320 ; 1001-0742
ISSN (online)	1878-7320
ISSN	1001-0742
DOI	10.1016/j.jes.2023.06.030
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Article ; Online: Short term exposure to polystyrene nanoplastics in mice evokes self-regulation of glycolipid metabolism.

He, Shiyu / Wang, Jingran / Zhou, Lihong / Jia, Tianjiang / Mao, Zhen / Zhang, Xiaodan / Zhang, Lingyan / Wang, Ji / Yang, Man / Huang, Peili

Ecotoxicology and environmental safety

2023 Volume 256, Page(s) 114906

Abstract: With the detection of nano-plastics (NPs) in daily essentials and drinking water, the potential harm of NPs to human health has become the focus of global attention. Studies have shown that long term exposure to NPs can lead to disorders of glucose and ... ...

Abstract	With the detection of nano-plastics (NPs) in daily essentials and drinking water, the potential harm of NPs to human health has become the focus of global attention. Studies have shown that long term exposure to NPs can lead to disorders of glucose and lipid metabolism in organisms, while the effects of short term exposure are rarely reported. Moreover, environmental factors cause the aging of NPs, and it is unclear whether this has an effect on their toxicity. In this study, we use 100 nm polystyrene (PS) NPs and ultraviolet (UV) aging PS (aPS) NPs to gavage mice for 7 days at an exposure dose of 50 mg/kg/day. To evaluate the effects of exposure on mice hepatic glucose lipid metabolism, we performed blood biochemical, pathological and metabolomic analyses. The results showed that exposure to PS NPs and aPS NPs increased serum glucose, disrupted serum lipoprotein levels, and up-regulated the expression levels of phosphatidylinositol 3-kinase (PI3K)/ phosphoprotein kinase B (p-AKT)/Glucose transporter 4 (GLUT4) proteins in the glucose metabolism pathway. The expression levels of key proteins sterol regulatory element binding protein-1 (SREBP-1)/peroxisome proliferator-activated receptor-γ (PPARγ)/adipose triglyceride lipase (ATGL) in the lipid metabolism signaling pathway were significantly increased. These findings suggest that short term exposure to PS NPs and aPS NPs induces glycolipid metabolism disturbance in mice, which may subsequently awaken the mice to self-regulate the serum levels of various lipoproteins and the expression of related key proteins. Compared with PS NPs, the aPS NPs interfered more strongly with glucose metabolism, and the corresponding self-regulation in mice was also more obvious. These findings not only provide a basis for environmental factors to increase the health risk of NPs but also provided a reference for the selection of test substances for further studies on the toxicity of NPs.
MeSH term(s)	Animals ; Humans ; Mice ; Glucose ; Glycolipids ; Lipid Metabolism ; Microplastics/metabolism ; Microplastics/toxicity ; Nanoparticles/toxicity ; Phosphatidylinositol 3-Kinases/metabolism ; Polystyrenes/toxicity ; Self-Control ; Environmental Pollutants/toxicity
Chemical Substances	Glucose (IY9XDZ35W2) ; Glycolipids ; Microplastics ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Polystyrenes ; Environmental Pollutants
Language	English
Publishing date	2023-04-14
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	436536-7
ISSN	1090-2414 ; 0147-6513
ISSN (online)	1090-2414
ISSN	0147-6513
DOI	10.1016/j.ecoenv.2023.114906
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Article ; Online: Parametric analysis of the output performance of a valveless piezoelectric pump with a bullhorn-shaped structure.

He, Lipeng / Hu, Dianbin / Wang, Jingran / Zhang, Zheng / Zhou, Ziming / Yu, Gang / Cheng, Guangming

The Review of scientific instruments

2021 Volume 92, Issue 7, Page(s) 75005

Abstract: In response to the serious problem of backflow in valveless piezoelectric pumps, this paper proposes a valveless piezoelectric pump with a bullhorn-shaped structure. In this paper, we analyze the flow guide by using the "tail flow space pressure ... ...

Abstract	In response to the serious problem of backflow in valveless piezoelectric pumps, this paper proposes a valveless piezoelectric pump with a bullhorn-shaped structure. In this paper, we analyze the flow guide by using the "tail flow space pressure comparison method" and "fluid unit dynamic analysis method," revealing the working principle of the flow guide in the valveless piezoelectric pump. The effects of the height, sharp angle, and filter angle of the bullhorn-shaped structure on the output flow were investigated separately by the experiment. The experimental results show that the output flow rate is best when the height of the bullhorn structure is 2 mm, the choke of the bullhorn-shaped structure is 2, and the sharp angle and fillet angle are 180°. The output flow rate of this valveless piezoelectric pump can reach 170.6 ml/min at a drive voltage of 210 V and a drive frequency of 45 Hz, indicating that the valveless piezoelectric pump has good pumping capability and can effectively alleviate the backflow phenomenon. This study offers some valuable insights into improving its performance and practical application.
Language	English
Publishing date	2021-08-03
Publishing country	United States
Document type	Journal Article
ZDB-ID	209865-9
ISSN	1089-7623 ; 0034-6748
ISSN (online)	1089-7623
ISSN	0034-6748
DOI	10.1063/5.0049776
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Article ; Online: Plant UVRAG interacts with ATG14 to regulate autophagosome maturation and geminivirus infection

Wang, Yan / Li, Jinlin / Wang, Jingran / Han, Ping / Miao, Shulei / Zheng, Xiyin / Han, Meng / Shen, Xueqi / Li, Huangai / Wu, Ming / Hong, Yiguo / Liu, Yule

New Phytologist. 2022 Nov., v. 236, no. 4 p.1358-1374

2022

Abstract: Autophagy is an essential degradation pathway that assists eukaryote survival under multiple stress conditions. Autophagosomes engulfing cargoes accomplish degradation only when they have matured through fusing with lysosomes or vacuoles. However, the ... ...

Abstract	Autophagy is an essential degradation pathway that assists eukaryote survival under multiple stress conditions. Autophagosomes engulfing cargoes accomplish degradation only when they have matured through fusing with lysosomes or vacuoles. However, the molecular machinery mediating autophagosome maturation in plants remains unknown. Using the combined approaches of mass spectrometry, biochemistry, reverse genetics and microscopy, we uncover that UVRAG, a subunit of the class III phosphatidylinositol 3‐kinase complexes in Nicotiana benthamiana, plays an essential role in autophagsome maturation via ATG14‐assisted recruitment to autophagosomes and by facilitating RAB7 activation. An interaction between N. benthamiana UVRAG and ATG14 was observed in vitro and in vivo, which strikingly differed from their mutually exclusive appearance in different PI3KC3 complexes in yeast and mammals. This interaction increased the localisation of UVRAG on autophagosomes and enabled the convergence of autophagic and late endosomal structures, where they contributed to fusions between these two types of organelles by recruiting the essential membrane fusion factors RAB7 GTPase and the homotypic fusion and protein sorting (HOPS) complex. In addition, we uncovered a joint contribution of ATG14 and UVRAG to geminiviral infection, beyond autophagy. Our study provides insights into the mechanisms of autophagosome maturation in plants and expands the understanding of organisations and roles of the PI3KC3 complexes.
Keywords	Geminiviridae ; Nicotiana benthamiana ; autophagosomes ; autophagy ; eukaryotic cells ; guanosinetriphosphatase ; lysosomes ; mass spectrometry ; membrane fusion ; microscopy ; phosphatidylinositol 3-kinase ; reverse genetics ; yeasts
Language	English
Dates of publication	2022-11
Size	p. 1358-1374.
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	208885-x
ISSN	1469-8137 ; 0028-646X
ISSN (online)	1469-8137
ISSN	0028-646X
DOI	10.1111/nph.18437
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Article ; Online: Source analysis of heavy metal pollution in agricultural soil irrigated with sewage in Wuqing, Tianjin.

Wang, Jingran / Yu, Danyang / Wang, Yanhong / Du, Xueli / Li, Guochen / Li, Bo / Zhao, Yujie / Wei, Yinghui / Xu, Shuang

Scientific reports

2021 Volume 11, Issue 1, Page(s) 17816

Abstract: In this study, the contents of heavy metals and Cd and Pb isotope ratios of agricultural soil and potential source samples collected from farmland receiving sewage irrigation in Wuqing District, Tianjin, China were determined. Multiple methods were used ... ...

Abstract	In this study, the contents of heavy metals and Cd and Pb isotope ratios of agricultural soil and potential source samples collected from farmland receiving sewage irrigation in Wuqing District, Tianjin, China were determined. Multiple methods were used for source analysis, including positive matrix factorization (PMF), correlation analysis, principal component analysis (PCA), and the Cd and Pb isotope ratio method. The results showed that agricultural soil was slightly contaminated by heavy metals in the research area, with relatively higher Cd and Pb accumulation levels compared to those of other heavy metals. Four types of pollution sources, including the soil parent material sources, industrial emission sources, agricultural practice sources, and mixed sources of sewage irrigation and transportation were apportioned and quantified by PMF, combined with the results of PCA and correlation analysis. The contribution rates quantified by the Cd and Pb isotope ratio method were similar, suggesting that no single source dominates Pb and Cd pollution. The contribution rates of Pb analyzed by the isotope ratio method were almost identical to those of the PMF model, indicating the rationality of the PMF result. Our results suggested that correlation analysis and PCA should be utilized to provide information for obtaining reasonable results and defining source categories for PMF, whereas the isotope ratio method should be applied to verify the accuracy of source contributions analyzed by PMF.
Language	English
Publishing date	2021-09-08
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-96367-8
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Article ; Online: Effects of astaxanthin on plasma membrane function and fertility of boar sperm during cryopreservation.

Guo, Hai-Tao / Wang, Jing-Ran / Sun, Liang-Zhen / Jin, Xiao-Hu / Shi, Xue-Ying / Lin, Jing-Yi / Yue, Shun-Li / Zhou, Jia-Bo

Theriogenology

2021 Volume 164, Page(s) 58–64

Abstract: Mammalian sperm is highly susceptible to reactive oxygen species (ROS) during the cryopreservation process. Astaxanthin (AST), a red pigment of the carotenoid family, is recognized as having a variety of beneficial biological activities and effects, ... ...

Abstract	Mammalian sperm is highly susceptible to reactive oxygen species (ROS) during the cryopreservation process. Astaxanthin (AST), a red pigment of the carotenoid family, is recognized as having a variety of beneficial biological activities and effects, including antioxidant, anticancer, anti-diabetic, and anti-inflammatory. The present study aimed to investigate whether the presence of AST protected boar sperm from ROS stress during cryopreservation. Boar sperm was diluted with a freezing medium supplemented with different concentrations of AST (0, 0.5, 1, 2, or 5 μM). The addition of AST, especially at a concentration of 2 μM, exerted positive effects on post-thaw sperm motility parameters. Meanwhile, sperm plasma membrane integrity and acrosome integrity of post-thaw sperm were significantly increased, while lipid peroxidation was inhibited in response to 2 μM AST treatment. Interestingly, compared to the control, supplementation with 2 μM AST increased unsaturated fatty acids (UFAs) levels and decreased saturated fatty acids (SFAs) content in post-thaw sperm, leading to a decreased ratio of SFAs/UFAs in the AST group. In conclusion, the addition of AST to freezing extenders inhibited lipid peroxidation and regulated fatty acid composition of the sperm membrane, improved post-thaw sperm quality, and had no adverse effect on boar sperm in vitro fertilization (IVF) capacity and potential for embryonic development. Our data provide a novel insight into understanding the mechanisms of AST concerning protecting boar sperm quality against ROS damage during cryopreservation.
MeSH term(s)	Animals ; Cell Membrane ; Cryopreservation/veterinary ; Cryoprotective Agents/pharmacology ; Fertility ; Male ; Semen Preservation/veterinary ; Sperm Motility ; Spermatozoa ; Swine ; Xanthophylls
Chemical Substances	Cryoprotective Agents ; Xanthophylls ; astaxanthine (8XPW32PR7I)
Language	English
Publishing date	2021-01-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	189232-0
ISSN	1879-3231 ; 0093-691X
ISSN (online)	1879-3231
ISSN	0093-691X
DOI	10.1016/j.theriogenology.2021.01.007
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Article: Effects of astaxanthin on plasma membrane function and fertility of boar sperm during cryopreservation

Guo, Hai-tao / Wang, Jing-ran / Sun, Liang-zhen / Jin, Xiao-hu / Shi, Xue-ying / Lin, Jing-yi / Yue, Shun-li / Zhou, Jia-bo

Theriogenology. 2021 Apr. 01, v. 164

2021

Abstract	Mammalian sperm is highly susceptible to reactive oxygen species (ROS) during the cryopreservation process. Astaxanthin (AST), a red pigment of the carotenoid family, is recognized as having a variety of beneficial biological activities and effects, including antioxidant, anticancer, anti-diabetic, and anti-inflammatory. The present study aimed to investigate whether the presence of AST protected boar sperm from ROS stress during cryopreservation. Boar sperm was diluted with a freezing medium supplemented with different concentrations of AST (0, 0.5, 1, 2, or 5 μM). The addition of AST, especially at a concentration of 2 μM, exerted positive effects on post-thaw sperm motility parameters. Meanwhile, sperm plasma membrane integrity and acrosome integrity of post-thaw sperm were significantly increased, while lipid peroxidation was inhibited in response to 2 μM AST treatment. Interestingly, compared to the control, supplementation with 2 μM AST increased unsaturated fatty acids (UFAs) levels and decreased saturated fatty acids (SFAs) content in post-thaw sperm, leading to a decreased ratio of SFAs/UFAs in the AST group. In conclusion, the addition of AST to freezing extenders inhibited lipid peroxidation and regulated fatty acid composition of the sperm membrane, improved post-thaw sperm quality, and had no adverse effect on boar sperm in vitro fertilization (IVF) capacity and potential for embryonic development. Our data provide a novel insight into understanding the mechanisms of AST concerning protecting boar sperm quality against ROS damage during cryopreservation.
Keywords	acrosome ; adverse effects ; astaxanthin ; boars ; cryopreservation ; embryogenesis ; fatty acid composition ; lipid peroxidation ; plasma membrane ; reactive oxygen species ; sperm motility ; sperm quality
Language	English
Dates of publication	2021-0401
Size	p. 58-64.
Publishing place	Elsevier Inc.
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	189232-0
ISSN	1879-3231 ; 0093-691X
ISSN (online)	1879-3231
ISSN	0093-691X
DOI	10.1016/j.theriogenology.2021.01.007
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Article: A 16S rDNA sequencing-based analysis of airway microecology in patients with an acute exacerbation of chronic obstructive pulmonary disease: A cross-sectional study in Inner Mongolia, China.

Zhu, Shu-Fen / Wu, Xin-Xin / Guo, Yan / Li, Peng-Fei / Wang, Jing-Ran / Liu, Miao / Luo, Cheng-Wen / Yuan, Xiang-Zhen / Li, Shao-Wei

Frontiers in medicine

2022 Volume 9, Page(s) 946238

Abstract: Aim: To study the microecological characteristics of the airway and similarities and differences between healthy people and patients with the acute exacerbation of chronic obstructive pulmonary disease (AECOPD) in Inner Mongolia, and analyze the ... ...

Abstract	Aim: To study the microecological characteristics of the airway and similarities and differences between healthy people and patients with the acute exacerbation of chronic obstructive pulmonary disease (AECOPD) in Inner Mongolia, and analyze the correlation between the characteristics of the airway microecological structure and clinical indicators of AECOPD patients. Methods: Sputum samples from 36 healthy volunteers and 34 patients with AECOPD were detected by 16S rDNA high-throughput sequencing, and the airway microecological characteristics of healthy people and AECOPD patients were revealed by an alpha diversity analysis, beta diversity analysis, and LefSe difference analysis. Results: There were differences in the airway microecological structure between healthy people and AECOPD patients in Inner Mongolia. The airway microbiota composition of AECOPD patients showed an increase in the abundance of common pathogens and a decrease in the abundance of commensal bacteria, and the airway microbial diversity in AECOPD patients was lower than that in healthy people. Long-term use of inhaled glucocorticoid + long-acting β2 agonist mixture (ICS + LABA), procalcitonin (PCT), blood monocyte count (MONO), hemoglobin (HGB), D-dimer (D-D), and body temperature were negatively correlated with the alpha diversity of the airway micro-ecosystem. Conclusion: The airway microecological composition of the AECOPD population in Inner Mongolia was different from that of the healthy population, and the airway microecological diversity was lower than that of the healthy population. The long-term use of ICS + LABA preparation by patients with AECOPD leads to lower alpha diversity. Alpha diversity was negatively correlated with inflammatory markers (PCT, MONO, D-dimer, body temperature) and HGB in AECOPD patients.
Language	English
Publishing date	2022-10-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2775999-4
ISSN	2296-858X
ISSN	2296-858X
DOI	10.3389/fmed.2022.946238
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