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Article ; Online: Structural characterization and anti-oxidative activity for a glycopeptide from Ganoderma lucidum fruiting body.

Luo, Hong-Jian / Zhang, Yu-Kun / Wang, Sai-Zhen / Lin, Shu-Qian / Wang, Lian-Fu / Lin, Zhan-Xi / Lu, Guo-Dong / Lin, Dong-Mei

International journal of biological macromolecules

2024 Volume 261, Issue Pt 2, Page(s) 129793

Abstract: A water-soluble glycopeptide (named GL- ... ...

Abstract	A water-soluble glycopeptide (named GL-PWQ
MeSH term(s)	Reishi/chemistry ; Spectroscopy, Fourier Transform Infrared ; Polysaccharides/chemistry ; Glucose/analysis ; Molecular Weight ; Water
Chemical Substances	Polysaccharides ; Glucose (IY9XDZ35W2) ; Water (059QF0KO0R)
Language	English
Publishing date	2024-01-28
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2024.129793
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Zs.B 2374: Show issues

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Article ; Online: Ganoderic acid alleviates chemotherapy-induced fatigue in mice bearing colon tumor.

Abulizi, Abudumijiti / Hu, Ling / Ma, Ang / Shao, Fang-Yu / Zhu, Hui-Ze / Lin, Si-Mei / Shao, Guang-Ying / Xu, Yue / Ran, Jian-Hua / Li, Jing / Zhou, Hong / Lin, Dong-Mei / Wang, Lian-Fu / Li, Min / Yang, Bao-Xue

Acta pharmacologica Sinica

2021 Volume 42, Issue 10, Page(s) 1703–1713

Abstract: Chemotherapy-related fatigue (CRF) is increasingly being recognized as one of the severe symptoms in patients undergoing chemotherapy, which not only largely reduces the quality of life in patients, but also diminishes their physical and social function. ...

Abstract	Chemotherapy-related fatigue (CRF) is increasingly being recognized as one of the severe symptoms in patients undergoing chemotherapy, which not only largely reduces the quality of life in patients, but also diminishes their physical and social function. At present, there is no effective drug for preventing and treating CRF. Ganoderic acid (GA), isolated from traditional Chinese medicine Ganoderma lucidum, has shown a variety of pharmacological activities such as anti-tumor, anti-inflammation, immunoregulation, etc. In this study, we investigated whether GA possessed anti-fatigue activity against CRF. CT26 tumor-bearing mice were treated with 5-fluorouracil (5-FU, 30 mg/kg) and GA (50 mg/kg) alone or in combination for 18 days. Peripheral and central fatigue-related behaviors, energy metabolism and inflammatory factors were assessed. We demonstrated that co-administration of GA ameliorated 5-FU-induced peripheral muscle fatigue-like behavior via improving muscle quality and mitochondria function, increasing glycogen content and ATP production, reducing lactic acid content and LDH activity, and inhibiting p-AMPK, IL-6 and TNF-α expression in skeletal muscle. Co-administration of GA also retarded the 5-FU-induced central fatigue-like behavior accompanied by down-regulating the expression of IL-6, iNOS and COX2 in the hippocampus through inhibiting TLR4/Myd88/NF-κB pathway. These results suggest that GA could attenuate 5-FU-induced peripheral and central fatigue in tumor-bearing mice, which provides evidence for GA as a potential drug for treatment of CRF in clinic.
MeSH term(s)	Animals ; Antineoplastic Agents/therapeutic use ; Cell Line, Tumor ; Colonic Neoplasms/drug therapy ; Colonic Neoplasms/pathology ; Cytokines/metabolism ; Energy Metabolism/drug effects ; Female ; Fluorouracil/adverse effects ; Fluorouracil/therapeutic use ; Hippocampus/drug effects ; Hippocampus/metabolism ; Mice, Inbred BALB C ; Muscle Fatigue/drug effects ; Muscle, Skeletal/drug effects ; Muscle, Skeletal/pathology ; Triterpenes/therapeutic use ; Mice
Chemical Substances	Antineoplastic Agents ; Cytokines ; Triterpenes ; ganoderic acid ; Fluorouracil (U3P01618RT)
Language	English
Publishing date	2021-04-29
Publishing country	United States
Document type	Journal Article
ZDB-ID	1360774-1
ISSN	1745-7254 ; 0253-9756 ; 1671-4083
ISSN (online)	1745-7254
ISSN	0253-9756 ; 1671-4083
DOI	10.1038/s41401-021-00669-6
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Article ; Online: The role of the A2a receptor agonist, regadenoson, in modulating hepatic artery flow in the porcine small-for-size liver graft.

Zhu, Xiaocheng / Shiba, Hiroaki / Fung, John J / Wang, Lian-Fu / Arakawa, Yusuke / Irefin, Samuel / Demetris, Anthony J / Kelly, Dympna M

The Journal of surgical research

2012 Volume 174, Issue 1, Page(s) e37–45

Abstract: Background: Hepatic artery vasoconstriction plays a major role in the pathophysiology of the small-for-size (SFS) liver graft injury and is reversed by adenosine. The A2a adenosine receptor (AR) has been suggested to be one of the key receptors that ... ...

Abstract	Background: Hepatic artery vasoconstriction plays a major role in the pathophysiology of the small-for-size (SFS) liver graft injury and is reversed by adenosine. The A2a adenosine receptor (AR) has been suggested to be one of the key receptors that modulate hepatic hemodynamic changes. The aim of the study is to define the effects of the A2a AR agonist, regadenoson, in modulating hepatic artery flow (HAF) in SFS liver grafts of a porcine model. Methods: Seven female recipient pigs (66-70 kg) receiving 20% liver grafts were treated with regadenoson, 0.1 ug/kg/min starting on POD1 (n = 7). Results were compared with those with untreated 20% liver grafts (n= 8). The recipients were observed for 14 d. Hepatic artery flow (HAF) and portal vein flow (PVF) were recorded. Liver biopsies and serum samples were also taken at the designed time points through postoperative day (POD)14. Results: Dose-response curves of regadenoson established 0.1 ug/kg/min as the most effective dose of regadenoson for maintaining an increase in HAF. No adverse effects were seen with regadenoson infusion. HAF immediately increased by up to 2.2-fold after regadenoson infusion. The levels of daily average of HAF and percentage of HAF in total liver blood flow were 34.5% and 41.8%, respectively, higher in the regadenoson group than in the untreated group. Histologic scores of hepatic artery spasm and bile duct necrosis were significantly lower in the regadenoson group than in the untreated group (P = 0.01 and 0.04, respectively). The complication rates of hepatic artery thrombosis and gastrointestinal bleeding were lower in the regadenoson group than in the untreated group (0/7, 0% versus 2/8, 25% and 0/7, 0% versus 2/8 and 25%, respectively). The 14-d survival rates were 4/7 (57.1 %) in regadenoson group compared with 2/8 (25%) in the untreated group. Conclusion: Adenosine A2a AR agonist, regadenoson, increases HAF in the recipients of SFS grafts with modest improvements in outcome.
MeSH term(s)	Adenosine A2 Receptor Agonists/pharmacology ; Animals ; Dose-Response Relationship, Drug ; Female ; Hepatic Artery/drug effects ; Hepatic Artery/physiology ; Liver/pathology ; Liver Circulation/drug effects ; Liver Transplantation ; Organ Size ; Postoperative Care ; Purines/pharmacology ; Pyrazoles/pharmacology ; Receptor, Adenosine A2A/physiology ; Survival Rate ; Swine
Chemical Substances	Adenosine A2 Receptor Agonists ; Purines ; Pyrazoles ; Receptor, Adenosine A2A ; regadenoson (2XLN4Y044H)
Language	English
Publishing date	2012-05-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80170-7
ISSN	1095-8673 ; 0022-4804
ISSN (online)	1095-8673
ISSN	0022-4804
DOI	10.1016/j.jss.2011.10.003
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Article: Normal rat hepatic stellate cells respond to endotoxin in LBP-independent manner to produce inhibitor(s) of DNA synthesis in hepatocytes.

Thirunavukkarasu, Chinnasamy / Uemura, Tadahiro / Wang, Lian Fu / Watkins, Simon C / Gandhi, Chandrashekhar R

Journal of cellular physiology

2005 Volume 204, Issue 2, Page(s) 654–665

Abstract: Endotoxin is implicated in the pathology of acute liver failure. The mechanisms of its actions on quiescent hepatic stellate cells (qHSCs) and their implications in hepatocyte injury are incompletely understood. We investigated effects of endotoxin ( ... ...

Abstract	Endotoxin is implicated in the pathology of acute liver failure. The mechanisms of its actions on quiescent hepatic stellate cells (qHSCs) and their implications in hepatocyte injury are incompletely understood. We investigated effects of endotoxin (bacterial lipopolysaccharide; LPS) on qHSCs and subsequently on hepatocytes. After overnight culture following their isolation, qHSCs were incubated with or without endotoxin for 24 h. The cells and the culture supernatant were analyzed for cytokines and nitric oxide (NO) synthesis. The effects of qHSC-conditioned media on hepatocytes were then determined. LPS increased inducible NO synthase expression, stimulated NO synthesis, and inhibited DNA synthesis in qHSCs. qHSC-conditioned medium inhibited DNA synthesis in hepatocytes without affecting NO synthesis, while LPS (1-1,000 ng/ml)-conditioned qHSC medium stimulated NO synthesis and caused further inhibition of DNA synthesis and apoptosis. These effects of LPS were more pronounced when qHSCs were incubated with serum, but not with LPS-binding protein (LBP) although CD14 (a receptor for LPS-LBP complex) was found in qHSCs. LPS stimulated the synthesis of TNF-alpha, interleukin (IL)-6, and IL-1beta but not of TGF-beta in qHSCs. Individually or together, L-N(G)-monomethylarginine and antibodies to IL-1beta, IL-6, and TNF-alpha only partly reversed qHSC + LPS-conditioned medium-induced inhibition of DNA synthesis in hepatocytes. These results suggest that the effects of LPS on qHSCs are novel, occurring without the aid of LBP/CD14. They also indicate that other factors, in addition to NO, TGF-beta, TNF-alpha, IL-1beta, and IL-6 are involved in the mechanisms of the growth inhibitory effects of qHSCs on hepatocytes.
MeSH term(s)	Acute-Phase Proteins/pharmacology ; Acute-Phase Proteins/physiology ; Animals ; Apoptosis/drug effects ; Carrier Proteins/pharmacology ; Carrier Proteins/physiology ; Cells, Cultured ; Culture Media, Conditioned/pharmacology ; Cyclic GMP/biosynthesis ; Cytokines/metabolism ; DNA/antagonists & inhibitors ; DNA/biosynthesis ; Endotoxins/pharmacology ; Enzyme Inhibitors/pharmacology ; Hepatocytes/drug effects ; Hepatocytes/metabolism ; Hepatocytes/physiology ; Lipopolysaccharide Receptors/metabolism ; Lipopolysaccharides/pharmacology ; Liver/cytology ; Liver/drug effects ; Male ; Membrane Glycoproteins/metabolism ; Membrane Glycoproteins/pharmacology ; Membrane Glycoproteins/physiology ; Nitric Oxide/biosynthesis ; Oxadiazoles/pharmacology ; Quinoxalines/pharmacology ; Rats ; Rats, Sprague-Dawley ; Receptors, Cell Surface/metabolism ; Toll-Like Receptors ; omega-N-Methylarginine/pharmacology
Chemical Substances	1H-(1,2,4)oxadiazolo(4,3-a)quinoxalin-1-one ; Acute-Phase Proteins ; Carrier Proteins ; Culture Media, Conditioned ; Cytokines ; Endotoxins ; Enzyme Inhibitors ; Lipopolysaccharide Receptors ; Lipopolysaccharides ; Membrane Glycoproteins ; Oxadiazoles ; Quinoxalines ; Receptors, Cell Surface ; Toll-Like Receptors ; lipopolysaccharide-binding protein ; omega-N-Methylarginine (27JT06E6GR) ; Nitric Oxide (31C4KY9ESH) ; DNA (9007-49-2) ; Cyclic GMP (H2D2X058MU)
Language	English
Publishing date	2005-08
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	3116-1
ISSN	1097-4652 ; 0021-9541
ISSN (online)	1097-4652
ISSN	0021-9541
DOI	10.1002/jcp.20366
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Article: Augmenter of liver regeneration: an important intracellular survival factor for hepatocytes.

Thirunavukkarasu, Chinnasamy / Wang, Lian Fu / Harvey, Stephen A K / Watkins, Simon C / Chaillet, J Richard / Prelich, John / Starzl, Thomas E / Gandhi, Chandrashekhar R

Journal of hepatology

2008 Volume 48, Issue 4, Page(s) 578–588

Abstract: Background/aims: Augmenter of liver regeneration (ALR), a protein synthesized and stored in hepatocytes, is associated with mitochondria, and possesses sulfhydryl oxidase and cytochrome c reductase activities. We sought to determine the effects of ALR ... ...

Abstract	Background/aims: Augmenter of liver regeneration (ALR), a protein synthesized and stored in hepatocytes, is associated with mitochondria, and possesses sulfhydryl oxidase and cytochrome c reductase activities. We sought to determine the effects of ALR depletion in hepatocytes by antisense oligonucleotide transfection. Methods: Rat hepatocytes in primary culture were transfected with antisense oligonucleotide for ALR mRNA (ALR-AS) or scrambled oligonucleotide. Various analyses were performed at times up to 24h after transfection. Results: Treatment with ALR-AS caused a decrease in ALR mRNA, cellular depletion of ALR protein primarily from mitochondria, and decreased viability. Flow cytometric analysis of ALR-AS-transfected hepatocytes stained with annexin-Vcy3 and 7-aminoactinomycin D revealed apoptosis as the predominant cause of death up to 6h; incubation beyond this time resulted in necrosis in addition to apoptosis. ALR-AS-transfection caused release of mitochondrial cytochrome c, activation of caspase-3, profound reduction in the ATP content, and cellular release of LDH. Inhibition of caspase-3 inhibited the early phase of ALR-AS-induced death but not the late phase that included ALR and LDH release. Conclusions: These results suggest that ALR is critically important for the survival of hepatocytes by its association with mitochondria and regulation of ATP synthesis.
MeSH term(s)	Adenosine Triphosphate/biosynthesis ; Animals ; Apoptosis ; Cell Count ; Cell Survival/physiology ; Cells, Cultured ; Flow Cytometry ; Hepatocytes/cytology ; Hepatocytes/metabolism ; Intracellular Fluid/metabolism ; Mitochondria, Liver/genetics ; Mitochondria, Liver/metabolism ; Oligonucleotides, Antisense/genetics ; Proteins/genetics ; Proteins/metabolism ; RNA, Messenger/genetics ; Rats ; Reverse Transcriptase Polymerase Chain Reaction ; Spectrophotometry ; Transfection
Chemical Substances	Oligonucleotides, Antisense ; Proteins ; RNA, Messenger ; GFER protein, rat (EC 1.8.-) ; Adenosine Triphosphate (8L70Q75FXE)
Language	English
Publishing date	2008-01-28
Publishing country	Netherlands
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605953-3
ISSN	1600-0641 ; 0168-8278
ISSN (online)	1600-0641
ISSN	0168-8278
DOI	10.1016/j.jhep.2007.12.010
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Article ; Online: Elevated catecholamines and hepatic artery vasospasm in porcine small-for-size liver graft.

Zhu, Xiaocheng / Fung, John J / Nakagawa, Shunichi / Wang, Lian-Fu / Irefin, Samuel / Cocieru, Andrei / Quintini, Cristiano / Diago, Teresa / Shiba, Hiroaki / Parra Sanchez, Ivan / Kelly, Dympna M

The Journal of surgical research

2012 Volume 174, Issue 1, Page(s) 157–165

Abstract: Background: Elevated levels of norepinephrine (NE) have been reported in recipients of small-for-size liver (SFS) grafts in the perioperative period. The aim of the study is to test the hypothesis that although circulating catecholamines are elevated in ...

Abstract	Background: Elevated levels of norepinephrine (NE) have been reported in recipients of small-for-size liver (SFS) grafts in the perioperative period. The aim of the study is to test the hypothesis that although circulating catecholamines are elevated in recipients of SFS grafts, they are not the primary agents responsible for the hepatic artery (HA) vasospasm. Methods: Female porcine recipients receiving a 20% (n = 10) partial liver graft were compared with a control group, using 60% partial liver transplanted grafts (n = 9). Hepatic blood flow (PVF, HAF) and levels of plasma catecholamines (epinephrine and NE) were measured at designated time points through postoperative day (POD) 7. Phentolamine (PA), an α-adrenergic blocker, was administered at doses of 1 to 112.5 ug/kg/min through an indwelling HA to the recipients of 20% group on POD1 (n = 5). Results: In the 20% group following reperfusion, HA vasospasm was found at 10, 60, and 90 min, and persisted on POD 3 and POD 7. Plasma NE levels increased after reperfusion in 20% and 60% groups and peaked at 6 h with 10- to 13-fold increased levels compared with baseline. In the 20% group, NE levels remained elevated up to POD 7. PA infusion at low (1-10 ug/kg/min) and high (12.5-112.5 ug/kg/min) doses did not reverse the reduced HAF observed in 20% group recipients. Conclusion: Elevated serum NE does not appear to be the primary factor mediating HA vasospasm in the porcine SFS graft.
MeSH term(s)	Animals ; Catecholamines/blood ; Female ; Hepatic Artery ; Liver/pathology ; Liver Circulation ; Liver Transplantation/adverse effects ; Organ Size ; Swine ; Vascular Diseases/etiology
Chemical Substances	Catecholamines
Language	English
Publishing date	2012-05-01
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80170-7
ISSN	1095-8673 ; 0022-4804
ISSN (online)	1095-8673
ISSN	0022-4804
DOI	10.1016/j.jss.2010.11.880
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Zs.A 178: Show issues

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Article ; Online: Adenosine restores the hepatic artery buffer response and improves survival in a porcine model of small-for-size syndrome.

Kelly, Dympna M / Zhu, Xiaocheng / Shiba, Hiroaki / Irefin, Samuel / Trenti, Loris / Cocieru, Andrei / Diago, Teresa / Wang, Lian Fu / Quintini, Cristiano / Chen, Zhong / Alster, Joan / Nakagawa, Shunichi / Miller, Charles / Demetris, Anthony / Fung, John J

Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society

2009 Volume 15, Issue 11, Page(s) 1448–1457

Abstract: The aim of the study is to define the role of the HABR in the pathophysiology of the SFS liver graft and to demonstrate that restoration of hepatic artery flow (HAF) has a significant impact on outcome and improves survival. Nine pigs received partial ... ...

Abstract	The aim of the study is to define the role of the HABR in the pathophysiology of the SFS liver graft and to demonstrate that restoration of hepatic artery flow (HAF) has a significant impact on outcome and improves survival. Nine pigs received partial liver allografts of 60% liver volume, Group 1; 8 animals received 20% LV grafts, Group 2; 9 animals received 20% LV grafts with adenosine infusion, Group 3. HAF and portal vein flow (PVF) were recorded at 10 min, 60 min and 90 min post reperfusion, on POD 3 and POD 7 in Group 1, and daily in Group 2 and 3 up to POD 14. Baseline HAF and PVF (ml/100 g/min) were 29 +/- 12 (mean +/- SD) and 74 +/- 8 respectively, with 28% of total liver blood flow (TLBF) from the HA and 72% from the PV. PVF peaked at 10 mins in all groups, increasing by a factor of 3.8 in the 20% group compared to an increase of 1.9 in the 60% group. By POD 7-14 PVF rates approached baseline values in all groups. The HABR was intact immediately following reperfusion in all groups with a reciprocal decrease in HAF corresponding to the peak PVF at 10 min. However in the 20% group HAF decreased to 12 +/- 8 ml/100 g/min at 90 min and remained low out to POD 7-14 despite restoration of normal PVF rates. Histopathology confirmed evidence of HA vasospasm and its consequences, cholestasis, centrilobular necrosis and biliary ischemia in Group 2. HA infusion of adenosine significantly improved HAF (p < .0001), reversed pathological changes and significantly improved survival (p = .05). An impaired HABR is important in the pathophysiology of the SFSS. Reversal of the vasospasm significantly improves outcome.
MeSH term(s)	Adenosine/pharmacology ; Animals ; Blood Pressure/drug effects ; Buffers ; Disease Models, Animal ; Female ; Graft Survival/drug effects ; Hepatic Artery ; Ischemia/drug therapy ; Ischemia/mortality ; Ischemia/pathology ; Kaplan-Meier Estimate ; Liver Circulation/drug effects ; Liver Transplantation ; Organ Size ; Postoperative Complications/drug therapy ; Postoperative Complications/mortality ; Postoperative Complications/pathology ; Swine ; Vasoconstriction/drug effects
Chemical Substances	Buffers ; Adenosine (K72T3FS567)
Language	English
Publishing date	2009-11
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2006866-9
ISSN	1527-6473 ; 1527-6465
ISSN (online)	1527-6473
ISSN	1527-6465
DOI	10.1002/lt.21863
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Zs.A 4702: Show issues

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Article: Augmentation of type-1 polarizing ability of monocyte-derived dendritic cells from chronically immunosuppressed organ-transplant recipients.

Macedo, Camila / Popescu, Iulia / Abu-Elmagd, Kareem / Reyes, Jorge / Shapiro, Ron / Zeevi, Adriana / Berghaus, Jan Mueller / Wang, Lian Fu / Lu, Lina / Thomson, Angus W / Storkus, Walter J / Fung, John J / Metes, Diana

Transplantation

2005 Volume 79, Issue 4, Page(s) 451–459

Abstract: Background: Chronic immunosuppressive (IS) therapy impairs normal T-cell immune surveillance and may predispose to opportunistic infections and malignancies that represent life-threatening complication of solid-organ transplantation (SOTx). Our study ... ...

Abstract	Background: Chronic immunosuppressive (IS) therapy impairs normal T-cell immune surveillance and may predispose to opportunistic infections and malignancies that represent life-threatening complication of solid-organ transplantation (SOTx). Our study was designed to ascertain the impact of chronic in vivo administration of IS on the ability of monocyte-derived dendritic cells (MoDC) to differentiate, mature, and function ex vivo. The potential of these cells to be implemented for DC-based adoptive immunotherapy was also considered. Methods: MoDCs were propagated by conventional procedures, their phenotype was analyzed by flow cytometry, and their function was assessed by mixed leukocyte reaction, enzyme-linked immunoadsorbent assay, and ELISPOT assays. Nuclear translocation of nuclear factor (NF)-kB was analyzed by electrophoretic mobility shift assay. Results: Circulating DC1s in peripheral blood were reduced in SOTx patients. MoDCs generated from patients displayed higher endocytic activity versus normal DCs, indicating their comparative immaturity. Patients' DCs exposed to pro-inflammatory cytokines (tumor necrosis factor-alpha, interleukin [IL]-1beta, and IL-6) were less able to mature, to stimulate recall antigen (Ag)- or allo-Ag-induced proliferation responses, or to secrete IL-12p70. These deficiencies were associated with a decrease in NF-kB translocation. In contrast, combination of pro-inflammatory cytokines and interferon (IFN)-gamma (a Th1-polarizing factor) augmented patients' DC1-type function and IL-12p70 production by way of an NF-kB-independent mechanism. Conclusions: Chronic IS restrains DC differentiation, maturation, and function at a transcriptional level; however, type-1 polarizing potential of patients' DC1 can be augmented ex vivo by a two-signal stimulation provided by pro-inflammatory cytokines and IFN-gamma. These results may have implications for DC-based immunotherapy of malignancies in the transplantation setting.
MeSH term(s)	Active Transport, Cell Nucleus ; Adult ; Antigens, CD ; CD11c Antigen/analysis ; Cell Differentiation/drug effects ; Cell Line ; Cell Polarity ; Cytokines/biosynthesis ; Dendritic Cells/drug effects ; Dendritic Cells/physiology ; HLA-DQ Antigens/metabolism ; Humans ; Immunoglobulins/analysis ; Immunosuppressive Agents/pharmacology ; Immunotherapy, Adoptive ; Lymphocyte Activation ; Membrane Glycoproteins/analysis ; Monocytes/cytology ; NF-kappa B/metabolism ; Organ Transplantation ; CD83 Antigen
Chemical Substances	Antigens, CD ; CD11c Antigen ; Cytokines ; HLA-DQ Antigens ; Immunoglobulins ; Immunosuppressive Agents ; Membrane Glycoproteins ; NF-kappa B
Language	English
Publishing date	2005-02-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	208424-7
ISSN	1534-6080 ; 0041-1337
ISSN (online)	1534-6080
ISSN	0041-1337
DOI	10.1097/01.tp.0000146589.49756.7f
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Zs.A 488: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article: Progression of liver fibrosis in patients with chronic hepatitis C after orthotopic liver transplantation.

Chopra, Kapil B / Demetris, Anthony J / Blakolmer, Karen / Dvorchik, Igor / Laskus, Tomasz / Wang, Lian-Fu / Araya, Victor R / Dodson, Forest / Fung, John J / Rakela, Jorge / Vargas, Hugo E

Transplantation

2003 Volume 76, Issue 10, Page(s) 1487–1491

Abstract: Background: Hepatitis C virus (HCV)-related cirrhosis is the leading indication for orthotopic liver transplantation (OLTx). HCV recurrence is universal after OLTx, with a highly variable course. This study aimed to find factors that affect progression ... ...

Abstract	Background: Hepatitis C virus (HCV)-related cirrhosis is the leading indication for orthotopic liver transplantation (OLTx). HCV recurrence is universal after OLTx, with a highly variable course. This study aimed to find factors that affect progression of fibrosis in recurrent HCV. Methods: Fifty-eight HCV patients underwent OLTx at our center who were selected on the basis of available preOLTx serum or explanted liver sample and liver biopsy obtained at least 6 months postOLTx. All liver biopsies were performed when clinically indicated and were scored using the modified Hepatitis Activity Index (HAI). Primary immunosuppression consisted of tacrolimus and prednisone. Results: The group included 41 males (mean age 49.6 years). HCV genotype distribution was 1a, 31 (53%); 1b, 16 (28%), and others 11 (19%). The mean follow-up was 53.1 months. Patients with genotype 1a (n=31; mean 46.3 months) had significantly lower fibrosis-free survival analyzed by the presence of fibrosis stages 5 and 6 when compared with other genotypes (n=27; mean 60.1 months; P=0.0088, log rank test). Mean HAI scores were significantly higher in HCV genotype 1a, although there were no differences in survival between genotypes. Similarly, patients with cytomegalovirus (CMV) infection postOLTx (n=4) had a higher fibrosis progression rate compared with those without CMV (n=54) (mean fibrosis-free survival 29.0 vs. 53.0 months P=0.0004, log-rank test). Human leukocyte antigen matching and rate of acute rejection did not influence progression of fibrosis. Conclusion: Patients with HCV genotype 1a and those developing CMV postOLTx have a higher rate of hepatic fibrosis progression after OLTx for HCV-related chronic liver disease.
MeSH term(s)	Adult ; Disease Progression ; Disease-Free Survival ; Genotype ; Hepacivirus/isolation & purification ; Hepatitis C, Chronic/surgery ; Humans ; Immunosuppression/methods ; Liver Cirrhosis/physiopathology ; Liver Transplantation/mortality ; Liver Transplantation/pathology ; Retrospective Studies ; Survival Analysis ; Time Factors
Language	English
Publishing date	2003-11-27
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	208424-7
ISSN	1534-6080 ; 0041-1337
ISSN (online)	1534-6080
ISSN	0041-1337
DOI	10.1097/01.TP.0000088668.28950.7C
Database	MEDical Literature Analysis and Retrieval System OnLINE

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