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Article ; Online: [Advances in nanobody screening technology].

Tang, Xiulan / Deng, Anqi / Chen, Wencong / Zhao, Yan / Wang, Maopeng / Li, Chang

Sheng wu gong cheng xue bao = Chinese journal of biotechnology

2024 Volume 40, Issue 2, Page(s) 350–366

Abstract: Nanobody (Nb) is a novel type of antibody discovered in the serum of Camelidae. It is characterized by its small size, high specificity, stability, and ease of preparation. Nanobodies exhibit the ability to identify hidden epitopes and have diverse ... ...

Abstract	Nanobody (Nb) is a novel type of antibody discovered in the serum of Camelidae. It is characterized by its small size, high specificity, stability, and ease of preparation. Nanobodies exhibit the ability to identify hidden epitopes and have diverse applications across various fields. This review aims to introduce three key stages in the screening and optimization of nanobodies, including nanobody library construction,
MeSH term(s)	Animals ; Single-Domain Antibodies/genetics ; Camelidae ; Cloning, Molecular ; Epitopes ; Saccharomyces cerevisiae/genetics
Chemical Substances	Single-Domain Antibodies ; Epitopes
Language	Chinese
Publishing date	2024-02-18
Publishing country	China
Document type	Review ; English Abstract ; Journal Article
ZDB-ID	1042206-7
ISSN	1872-2075 ; 1042-749X
ISSN (online)	1872-2075
ISSN	1042-749X
DOI	10.13345/j.cjb.230472
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Article ; Online: Effectiveness of an online management platform (Joint Cloud) versus standard process for patients undergoing total knee arthroplasty: study protocol for a prospective randomised controlled trial.

Zhang, Shuhao / Ma, He / Wang, Lei / Wang, Maopeng / Li, Bing / Liu, Jun

BMJ open

2023 Volume 13, Issue 11, Page(s) e073058

Abstract: Introduction: Osteoarthritis (OA) is one of the main causes of mobility impairment in the elderly worldwide. Therefore, total knee arthroplasty (TKA) is often performed and is one of the most successful surgery and has resulted in substantial quality-of- ...

Abstract	Introduction: Osteoarthritis (OA) is one of the main causes of mobility impairment in the elderly worldwide. Therefore, total knee arthroplasty (TKA) is often performed and is one of the most successful surgery and has resulted in substantial quality-of-life gains for people with end-stage arthritis. There is still room for improvement in the standard treatment process in the preoperative, intraoperative and postoperative period of TKA. Telerehabilitation has the potential to become a positive alternative to face-to-face rehabilitation nowadays. But it remains unclear how well telemedicine interventions cover the entire surgical pathway (preoperation, intraoperation, postoperation). This study aims to explore the effectiveness of Joint Cloud (JC, an online management platform) compared with existing standard process in regulating functional recovery, pain management, muscle strength changes and other health-related outcomes in patients undergoing total knee arthroplasty preoperation, intraoperation and postoperation. Methods and analysis: A randomised controlled trial was designed to compare the online management platform (JC) with standard process (SP) in patients undergoing TKA. A total of 186 TKA patients will be randomly assigned to the intervention (n=93) or control (n=93) group. Patients in the intervention group will receive access to the 'JC' mini-program. This mini-program provides popular science information (eg, information about OA and TKA), functional exercise information and communication channels. Patients evaluate their condition and functional level through standardised digital questionnaires. The control group of patients will not accept any functions of this mini-program. The primary outcome is knee functional recovery, and the secondary outcomes are pain management, isometric knee extensor muscle strength, patient satisfaction and cost-benefit analysis. Assessments will be performed 1 month and 3 days before surgery (T0) and 1 month and 3 months after surgery. Data analysis will be performed according to the intent-to-treat (ITT) principle. Repeated measures of linear mixed models and parametric and non-parametric testing will be used for statistical analysis. Ethics and dissemination: The study was reviewed and approved by the Tianjin Hospital Medical Ethics Review Committee on 10 February 2023 (2022YLS155). Test data are considered highly sensitive but are available upon request. The findings will be disseminated in peer-reviewed publications. Trial registration number: ChiCTR2300068486.
MeSH term(s)	Humans ; Aged ; Arthroplasty, Replacement, Knee/rehabilitation ; Osteoarthritis, Knee/surgery ; Prospective Studies ; Knee Joint/surgery ; Patient Satisfaction ; Treatment Outcome ; Randomized Controlled Trials as Topic
Language	English
Publishing date	2023-11-23
Publishing country	England
Document type	Clinical Trial Protocol ; Journal Article
ZDB-ID	2599832-8
ISSN	2044-6055 ; 2044-6055
ISSN (online)	2044-6055
ISSN	2044-6055
DOI	10.1136/bmjopen-2023-073058
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Article ; Online: Transcriptomic Analysis of PDCoV-Infected HIEC-6 Cells and Enrichment Pathways PI3K-Akt and P38 MAPK.

Jiang, Yuhang / Zhang, Guoqing / Li, Letian / Wang, Maopeng / Chen, Jing / Hao, Pengfei / Gao, Zihan / Hao, Jiayi / Li, Chang / Jin, Ningyi

Viruses

2024 Volume 16, Issue 4

Abstract: Porcine Deltacoronavirus (PDCoV) is a newly identified coronavirus that causes severe intestinal lesions in piglets. However, the understanding of how PDCoV interacts with human hosts is limited. In this study, we aimed to investigate the interactions ... ...

Abstract	Porcine Deltacoronavirus (PDCoV) is a newly identified coronavirus that causes severe intestinal lesions in piglets. However, the understanding of how PDCoV interacts with human hosts is limited. In this study, we aimed to investigate the interactions between PDCoV and human intestinal cells (HIEC-6) by analyzing the transcriptome at different time points post-infection (12 h, 24 h, 48 h). Differential gene analysis revealed a total of 3560, 5193, and 4147 differentially expressed genes (DEGs) at 12 h, 24 h, and 48 h, respectively. The common genes among the DEGs at all three time points were enriched in biological processes related to cytokine production, extracellular matrix, and cytokine activity. KEGG pathway analysis showed enrichment of genes involved in the p53 signaling pathway, PI3K-Akt signaling pathway, and TNF signaling pathway. Further analysis of highly expressed genes among the DEGs identified significant changes in the expression levels of BUB1, DDIT4, ATF3, GBP2, and IRF1. Comparison of transcriptome data at 24 h with other time points revealed 298 DEGs out of a total of 6276 genes. KEGG analysis of these DEGs showed significant enrichment of pathways related to viral infection, specifically the PI3K-Akt and P38 MAPK pathways. Furthermore, the genes EFNA1 and KITLG, which are associated with viral infection, were found in both enriched pathways, suggesting their potential as therapeutic or preventive targets for PDCoV infection. The enhancement of PDCoV infection in HIEC-6 was observed upon inhibition of the PI3K-Akt and P38 MAPK signaling pathways using sophoridine. Overall, these findings contribute to our understanding of the molecular mechanisms underlying PDCoV infection in HIEC-6 cells and provide insights for developing preventive and therapeutic strategies against PDCoV infection.
MeSH term(s)	Humans ; Animals ; Proto-Oncogene Proteins c-akt/metabolism ; Proto-Oncogene Proteins c-akt/genetics ; Gene Expression Profiling ; Phosphatidylinositol 3-Kinases/metabolism ; Phosphatidylinositol 3-Kinases/genetics ; Signal Transduction ; Swine ; Transcriptome ; p38 Mitogen-Activated Protein Kinases/metabolism ; p38 Mitogen-Activated Protein Kinases/genetics ; Deltacoronavirus/genetics ; Cell Line ; Coronavirus Infections/virology ; Coronavirus Infections/genetics ; Swine Diseases/virology ; Swine Diseases/genetics ; Host-Pathogen Interactions/genetics
Chemical Substances	Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; p38 Mitogen-Activated Protein Kinases (EC 2.7.11.24)
Language	English
Publishing date	2024-04-09
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v16040579
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Article: A micro-sized vaccine based on recombinant

Li, Letian / Hao, Jiayi / Jiang, Yuhang / Hao, Pengfei / Gao, Yuwei / Chen, Jing / Zhang, Guoqing / Jin, Ningyi / Wang, Maopeng / Li, Chang

Acta pharmaceutica Sinica. B

2023

Abstract: COVID-19 has globally spread to burden the medical system. Even with a massive vaccination, a mucosal vaccine offering more comprehensive and convenient protection is imminent. Here, a micro-sized vaccine based on ... ...

Abstract	COVID-19 has globally spread to burden the medical system. Even with a massive vaccination, a mucosal vaccine offering more comprehensive and convenient protection is imminent. Here, a micro-sized vaccine based on recombinant
Language	English
Publishing date	2023-02-23
Publishing country	Netherlands
Document type	Journal Article
ISSN	2211-3835
ISSN	2211-3835
DOI	10.1016/j.apsb.2023.01.005
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Article ; Online: Genomic characteristics of an avipoxvirus 282E4 strain.

Deng, Lingcong / Liu, Cunxia / Li, Letian / Hao, Pengfei / Wang, Maopeng / Jin, Ningyi / Yin, Ronglan / Du, Shouwen / Li, Chang

Virus research

2023 Volume 336, Page(s) 199218

Abstract: Avipoxvirus 282E4 strain was extensively applied into recombinant vaccine vector to prevent other infectious diseases. However, little information on the genomic background, functional and genetic evolutionary of the isolate 282E4 strain was clarified. ... ...

Abstract	Avipoxvirus 282E4 strain was extensively applied into recombinant vaccine vector to prevent other infectious diseases. However, little information on the genomic background, functional and genetic evolutionary of the isolate 282E4 strain was clarified. The results showed that the linear genome of avipoxvirus 282E4 was 308,826 bp, containing 313 open reading frames (ORFs) and 12 new predicted ORFs. The 282E4 strain appears to encode two novel thymidine kinase proteins and two TGF-beta-like proteins that may be associated with the suppression of the host's antiviral response. Avipoxvirus 282E4 also encodes 57 ankyrin repeat proteins and 5 variola B22R-like proteins, which composed 7% of the avipoxvirus 282E4 genome. GO and KEGG analysis further revealed that 12 ORFs participate in viral transcription process, 7 ORFs may function during DNA repair, replication and biological synthesis, and ORF 208 is involved in the process of virus life cycle. Interestingly, phylogenetic analysis based on concatenated sequences p4b and DNA polymerase of avipoxviruses gene demonstrates that avipoxvirus 282E4 strain is divergent from known FWPV isolates and is similar to shearwater poxvirus (SWPV-1) that belongs to the CNPV-like virus. Sequencing avipoxvirus 282E4 is a significant step to judge the genetic position of avipoxviruses within the larger Poxviridae phylogenetic tree and provide a new insight into the genetic background of avipoxvirus 282E4 and interspecies transmission of poxviruses, meanwhile, explanation of gene function provides theoretical foundation for vaccine design with 282E4 strain as skeleton.
Language	English
Publishing date	2023-09-13
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	605780-9
ISSN	1872-7492 ; 0168-1702
ISSN (online)	1872-7492
ISSN	0168-1702
DOI	10.1016/j.virusres.2023.199218
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Article ; Online: Nanobodies against porcine CD163 as PRRSV broad inhibitor.

Deng, Zhuofan / Zhang, Shengkun / Sun, Meiqi / Yang, Haotian / Lu, Ying / Wang, Maopeng / Fang, Weihuan / Shi, Fushan / He, Fang

International journal of biological macromolecules

2023 Volume 253, Issue Pt 7, Page(s) 127493

Abstract: PRRSV (Porcine Reproductive and Respiratory Syndrome Virus) is a major swine pathogen causing economic losses. To the date, effective broad PRRSV inhibitory strategies have not been available in practice yet. Targeting the key viral receptor CD163 to ... ...

Abstract	PRRSV (Porcine Reproductive and Respiratory Syndrome Virus) is a major swine pathogen causing economic losses. To the date, effective broad PRRSV inhibitory strategies have not been available in practice yet. Targeting the key viral receptor CD163 to block PRRSV entry has emerged as an alternative approach beside vaccines for PRRSV inhibition. As an effective therapeutic tool, nanoantibodies (Nbs) have been widely used in antiviral research. In this study, a phage display VHH library was constructed for the selection of Nbs against porcine CD163 scavenger receptor cysteine-rich 5-9 domain (SRCR5-9). After five rounds of bio-panning and indirect ELISA, seven CD163-specific Nbs (Nb1-Nb7) were identified. All obtained Nbs displayed strong affinity to CD163 receptor and excellent antiviral activity. In particular, Nb2 exhibited significant broad inhibitory effects on variable PRRSV lineages and downregulated virus-related NF-κB signaling. Further studies suggested that Nbs mainly exerted antiviral functions by interfering with virus attachment stage, and also decreased the transcription of CD163. The conformational epitopes recognized by Nbs were localized in the SRCR5 domain of CD163, a crucial region in PRRSV infection. Overall, our findings provide a novel insight into the biofunction of CD163 in antiviral infection and the development of broad-spectrum strategies against PRRSV.
MeSH term(s)	Swine ; Animals ; Porcine respiratory and reproductive syndrome virus ; Single-Domain Antibodies/pharmacology ; Porcine Reproductive and Respiratory Syndrome ; Antiviral Agents/pharmacology
Chemical Substances	CD163 antigen ; Single-Domain Antibodies ; Antiviral Agents
Language	English
Publishing date	2023-10-17
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2023.127493
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Article ; Online: Association between matrix metalloproteinase-1 (MMP-1) protein level and the risk of rheumatoid arthritis and osteoarthritis: a meta-analysis.

Wang, Maopeng / Zhou, You / Huang, Wei / Zeng, Yong / Li, Xinzhi

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas

2020 Volume 54, Issue 2, Page(s) e10366

Abstract: Recent publications have investigated the potential role of the protein level of matrix metalloproteinase-1 (MMP-1) in the susceptibility to rheumatoid arthritis (RA) and osteoarthritis (OA). However, no unanimous conclusion was obtained. Therefore, we ... ...

Abstract	Recent publications have investigated the potential role of the protein level of matrix metalloproteinase-1 (MMP-1) in the susceptibility to rheumatoid arthritis (RA) and osteoarthritis (OA). However, no unanimous conclusion was obtained. Therefore, we carried out a meta-analysis to explore the association between MMP-1 expression and these two clinical disorders. After database searching and screening, we enrolled a total of eighteen articles for the pooled analysis. We observed a significant association between RA cases and controls in the whole population [SMD (standard mean difference)=1.01, P=0.017]. There were similar positive results in the subgroup analysis of "population-based control" (SMD=1.50, P=0.032) and "synovial fluid" (SMD=1.32, P=0.049). In addition, we observed an increased risk in OA cases, compared with controls, in the overall analysis (SMD=0.47, P=0.004) and subsequent subgroup analysis of "knee OA" (SMD=0.86, P<0.001), "Asian/China" (SMD=0.76, P=0.003), "cartilage-Asian/China" (SMD=1.21, P<0.001), and "synovial fluid-Asian/China" (SMD=0.73, P=0.004). In summary, a high protein level of MMP-1 in synovial fluid may be associated with the susceptibility to RA, and the high MMP-1 level in the cartilage tissue or synovial fluid may be related to the pathogenesis of knee OA in the Chinese population. This should be confirmed by larger sample sizes.
MeSH term(s)	Arthritis, Rheumatoid/genetics ; Humans ; Matrix Metalloproteinase 1/genetics ; Osteoarthritis, Knee/genetics ; Synovial Fluid
Chemical Substances	MMP1 protein, human (EC 3.4.24.7) ; Matrix Metalloproteinase 1 (EC 3.4.24.7)
Language	English
Publishing date	2020-12-09
Publishing country	Brazil
Document type	Journal Article ; Meta-Analysis
ZDB-ID	786234-9
ISSN	1414-431X ; 0100-879X
ISSN (online)	1414-431X
ISSN	0100-879X
DOI	10.1590/1414-431X202010366
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Article ; Online: Ferroptosis in viral infection: the unexplored possibility.

Wang, Mao-Peng / Joshua, Banda / Jin, Ning-Yi / Du, Shou-Wen / Li, Chang

Acta pharmacologica Sinica

2021 Volume 43, Issue 8, Page(s) 1905–1915

Abstract: Virus-induced cell death has long been thought of as a double-edged sword in the inhibition or exacerbation of viral infections. The vital role of iron, an essential element for various enzymes in the maintenance of cellular physiology and efficient ... ...

Abstract	Virus-induced cell death has long been thought of as a double-edged sword in the inhibition or exacerbation of viral infections. The vital role of iron, an essential element for various enzymes in the maintenance of cellular physiology and efficient viral replication, places it at the crossroads and makes it a micronutrient of competition between the viruses and the host. Viruses can interrupt iron uptake and the antioxidant response system, while others can utilize iron transporter proteins as receptors. Interestingly, the unavailability of iron facilitates certain viral infections and causes cell death characterized by lipid peroxide accumulation and malfunction of the antioxidant system. In this review, we discuss how iron uptake, regulation and metabolism, including the redistribution of iron in the host defense system during viral infection, can induce ferroptosis. Fenton reactions, a central characteristic of ferroptosis, are caused by the increased iron content in the cell. Therefore, viral infections that increase cellular iron content or intestinal iron absorption are likely to cause ferroptosis. In addition, we discuss the hijacking of the iron regulatoy pathway and the antioxidant response, both of which are typical in viral infections. Understanding the potential signaling mechanisms of ferroptosis in viral infections will aid in the development of new therapeutic agents.
MeSH term(s)	Antioxidants/metabolism ; Antioxidants/therapeutic use ; Cell Death ; Ferroptosis ; Humans ; Iron/metabolism ; Lipid Peroxidation ; Virus Diseases
Chemical Substances	Antioxidants ; Iron (E1UOL152H7)
Language	English
Publishing date	2021-12-06
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	1360774-1
ISSN	1745-7254 ; 0253-9756 ; 1671-4083
ISSN (online)	1745-7254
ISSN	0253-9756 ; 1671-4083
DOI	10.1038/s41401-021-00814-1
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Article: A recombinant Lactobacillus plantarum strain expressing the spike protein of SARS-CoV-2

Wang, Maopeng / Fu, Tingting / Hao, Jiayi / Li, Letian / Tian, Mingyao / Jin, Ningyi / Ren, Linzhu / Li, Chang

International journal of biological macromolecules. 2020 Oct. 01, v. 160

2020

Abstract: Coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a global pandemic in the past four months and causes respiratory disease in humans of almost all ages. Although several drugs ...

Abstract	Coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a global pandemic in the past four months and causes respiratory disease in humans of almost all ages. Although several drugs have been announced to be partially effective treatments for this disease, no approved vaccine is available. Here, we described the construction of a recombinant Lactobacillus plantarum strain expressing the SARS-CoV-2 spike protein. The results showed that the spike gene with optimized codons could be efficiently expressed on the surface of recombinant L. plantarum and exhibited high antigenicity. The highest protein yield was obtained under the following conditions: cells were induced with 50 ng/mL SppIP at 37 °C for 6–10 h. The recombinant spike (S) protein was stable under normal conditions and at 50 °C, pH = 1.5, or a high salt concentration. Recombinant L. plantarum may provide a promising food-grade oral vaccine candidate against SARS-CoV-2 infection.
Keywords	COVID-19 infection ; Lactobacillus plantarum ; Severe acute respiratory syndrome coronavirus 2 ; codons ; edible vaccines ; pH ; pandemic ; respiratory tract diseases ; salt concentration
Language	English
Dates of publication	2020-1001
Size	p. 736-740.
Publishing place	Elsevier B.V.
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2020.05.239
Database	NAL-Catalogue (AGRICOLA)

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Article: Construction and optimization of Lactobacillus plantarum expression system expressing glycoprotein 5 of porcine reproductive and respiratory syndrome virus

Wang, Maopeng / Du, Shouwen / Xu, Wang / Song, Lina / Hao, Pengfei / Jin, Ningyi / Ren, Linzhu / Li, Chang

International journal of biological macromolecules. 2020 Jan. 15, v. 143

2020

Abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) causes serious reproductive failure and respiratory disease in pigs. Although numerous vaccines were developed against the virus, licensed vaccines showed limited efficacy. Here, we describe the ...

Abstract	Porcine reproductive and respiratory syndrome virus (PRRSV) causes serious reproductive failure and respiratory disease in pigs. Although numerous vaccines were developed against the virus, licensed vaccines showed limited efficacy. Here, we describe the construction and optimization of Lactobacillus plantarum expression system of PRRSV GP5 gene. The wild-type truncated GP5 or codon-optimized truncated GP5 was linked with endogenous signal peptide and target peptides (DCpep or Mpep) at 5′ and 3′ end of the gene, respectively. Then, the fragments were cloned into the L. plantarum expression plasmid pSIP411 and expressed under the induction of SppIP. As a result, PRRSV GP5 genes with optimized codons have higher expressions than that of the GP5 genes with wild-type codons, indicating codons optimization is an effective way to enhance the expression of an exogenous gene in L. plantarum. Further analysis showed that the codon-optimized GP5 with endogenous signal peptide can be effectively displayed on the surface of the L. plantarum, and the GP5 harboring target peptide Mpep displayed the highest antigenicity than the others. The highest production of PRRSV GP5 was obtained under the following conditions: L. plantarum harboring the plasmid pSIP-1320-O5MH are induced with 200 ng/mL SppIP at 33 °C for 7 h.
Keywords	Lactobacillus plantarum ; Porcine reproductive and respiratory syndrome virus ; codons ; glycoproteins ; peptides ; plasmids ; respiratory tract diseases ; signal peptide ; swine ; swine diseases ; vaccines ; viruses
Language	English
Dates of publication	2020-0115
Size	p. 112-117.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2019.12.006
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