LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 5 of total 5

Search options

  1. Article ; Online: Structural characterization and anti-oxidative activity for a glycopeptide from Ganoderma lucidum fruiting body.

    Luo, Hong-Jian / Zhang, Yu-Kun / Wang, Sai-Zhen / Lin, Shu-Qian / Wang, Lian-Fu / Lin, Zhan-Xi / Lu, Guo-Dong / Lin, Dong-Mei

    International journal of biological macromolecules

    2024  Volume 261, Issue Pt 2, Page(s) 129793

    Abstract: A water-soluble glycopeptide (named GL- ... ...

    Abstract A water-soluble glycopeptide (named GL-PWQ
    MeSH term(s) Reishi/chemistry ; Spectroscopy, Fourier Transform Infrared ; Polysaccharides/chemistry ; Glucose/analysis ; Molecular Weight ; Water
    Chemical Substances Polysaccharides ; Glucose (IY9XDZ35W2) ; Water (059QF0KO0R)
    Language English
    Publishing date 2024-01-28
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2024.129793
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 2374: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article: [Effect of Ganoderma lucidum polysaccharides peptide on invasion of human lung carcinoma cells in vitro].

    Cao, Qi Zhen / Lin, Shu Qian / Wang, Sai Zhen

    Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences

    2007  Volume 39, Issue 6, Page(s) 653–656

    Abstract: Objective: To find out the effects of Ganoderma lucidum polysaccharides peptide (Gl-PP) on the invasion of the human lung carcinoma cell (PG cell).: Methods: PG cells were pretreated with different concentration Gl-PP in vitro, using cell ... ...

    Abstract Objective: To find out the effects of Ganoderma lucidum polysaccharides peptide (Gl-PP) on the invasion of the human lung carcinoma cell (PG cell).
    Methods: PG cells were pretreated with different concentration Gl-PP in vitro, using cell proliferation assay, cell migration assay, adhesion assay, zymography and RT-PCR, then the effects of Gl-PP on proliferation, motility, adhesion and MMP-9 activity and mRNA expression of PG cells were investigated in vitro.
    Results: Gl-PP did not directly inhibit PG cell proliferation in vitro. However, pretreated with Gl-PP, PG cells motility was inhibited significantly. PG cells adhesion was also inhibited. The activity of MMP-9 was inhibited in a dose-dependent manner, and the inhibited ratio was 41.53% at a dose of 100 mg/L Gl-PP. The mRNA expression of MMP-9 of pretreated PG cells was inhibited.
    Conclusion: Gl-PP could suppress invasion of human lung carcinoma cells in vitro.
    MeSH term(s) Cell Adhesion/drug effects ; Cell Line, Tumor ; Cell Movement/drug effects ; Cell Proliferation/drug effects ; Humans ; Lung Neoplasms/metabolism ; Lung Neoplasms/pathology ; Matrix Metalloproteinase 9/metabolism ; Neoplasm Invasiveness ; Proteoglycans/pharmacology ; Reishi
    Chemical Substances Proteoglycans ; polysaccharide peptide ; MMP9 protein, human (EC 3.4.24.35) ; Matrix Metalloproteinase 9 (EC 3.4.24.35)
    Language Chinese
    Publishing date 2007-12-18
    Publishing country China
    Document type English Abstract ; Journal Article
    ISSN 1671-167X
    ISSN 1671-167X
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article: [Isolation, purification and structural analysis of GL-PP-3A, an active polysaccharide peptide from Ganoderma lucidum].

    Wang, Sai-zhen / Ding, Kan / Lin, Shu-qian / Lin, Zhi-bin

    Yao xue xue bao = Acta pharmaceutica Sinica

    2007  Volume 42, Issue 10, Page(s) 1058–1061

    Abstract: GL-PP-3A, an active polysaccharide peptide, was isolated and purified from Ganoderma lucidum, and then its structure was analyzed. Crude polysaccharide peptides were extracted from Ganoderma lucidum with hot water, precipitated with ethanol and then ... ...

    Abstract GL-PP-3A, an active polysaccharide peptide, was isolated and purified from Ganoderma lucidum, and then its structure was analyzed. Crude polysaccharide peptides were extracted from Ganoderma lucidum with hot water, precipitated with ethanol and then dialyzed from Ganoderma lucidum. Subsequently GL-PP-3A was isolated and purified from the crude polysaccharide peptides by fractional precipitation and chromatography of Bio-Gel P-10 column. The repetitive unit of GL-PP-3A was analyzed by high performance gel permeation chromatography (HPGPC), monosaccharide composition and methylation analysis, 1H NMR and 13C NMR. GL-PP-3A is a heteropolysaccharide which is composed mainly of glucose (Glc), and also contains saccharide residues such as rhamnose (Rha), xylose (Xyl), mannose (Man) and galactose (Gal) and 17 kinds of amino acids. Its weight-average molecular weight (Mw) and number-average molecular weight (Mn) were 1.7 x 10(4) and 1.1 x 10(4), respectively, with the ratio of Mw/Mn ( molecular weight distribution) being of 1.49. Its backbone chain is composed of 1,6-linked beta-D-Glcp and 1,3-liked beta-D-Glcp at a ratio of 2:1. Some of 1,6-linked glucose residuals of the backbone chain are substituted at 2-0 or 3-0, and there are 1 to 3 1,6-linked beta-D-Galp or 1,3-linked alpha-D-Manp in the branched chains, the nonreducing ends of which consist mainly of beta-D-Glcp and a few Rha.
    MeSH term(s) Amino Acids/analysis ; Chromatography, High Pressure Liquid ; Glucose/analysis ; Magnetic Resonance Spectroscopy ; Molecular Weight ; Plants, Medicinal/chemistry ; Polysaccharides/chemistry ; Polysaccharides/isolation & purification ; Proteoglycans/chemistry ; Proteoglycans/isolation & purification ; Reishi/chemistry ; Rhamnose/analysis ; Spectrophotometry, Ultraviolet
    Chemical Substances Amino Acids ; Polysaccharides ; Proteoglycans ; polysaccharide peptide ; Glucose (IY9XDZ35W2) ; Rhamnose (QN34XC755A)
    Language Chinese
    Publishing date 2007-10
    Publishing country China
    Document type English Abstract ; Journal Article
    ZDB-ID 788758-9
    ISSN 0513-4870
    ISSN 0513-4870
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 3194: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article: [Comparison of the immunomodulatory effects of spore polysaccharides and broken spore polysaccharides isolated from Ganoderma lucidum on murine splenic lymphocytes and peritoneal macrophages in vitro].

    Wang, Peng-yun / Wang, Sai-zhen / Lin, Shu-qian / Lin, Zhi-bin

    Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences

    2005  Volume 37, Issue 6, Page(s) 569–574

    Abstract: Objective: To compare the immunomodulatory effects of spore polysaccharides (Gl-SP) and broken spore polysaccharides (Gl-BSP) isolated from Ganoderma lucidum(Leyss et Fr.) Karst. on murine splenic lymphocytes and peritoneal macrophages in vitro.: ... ...

    Abstract Objective: To compare the immunomodulatory effects of spore polysaccharides (Gl-SP) and broken spore polysaccharides (Gl-BSP) isolated from Ganoderma lucidum(Leyss et Fr.) Karst. on murine splenic lymphocytes and peritoneal macrophages in vitro.
    Methods: Mixed lymphocyte culture reaction (MLR), lymphocyte proliferation in the presence or absence of mitogen, and the cytotoxic activity of splenic natural killer (NK) cells were detected with MTT assay in vitro. The percentage of phagocytosis of neutral red (NR) by mouse peritoneal macrophages was detected by colorimetric assay. Splenic T-lymphocyte subpopulations were measured with flow cytometry(FCM). IL-2, IFN-gamma and TNF-alpha in the culture supernatants were detected by ELISA and biological assay. Nitric oxide (NO) production was examined by Griess reaction.
    Results: At the concentration range of 0.2-12.8 mg/L, Gl-SP and Gl-BSP were shown to increase lymphocyte proliferation in the presence or absence of mitogen, enhance NK cytotoxic activity, augment the production of TNF-alpha and NO in Gl-SP- or Gl-BSP-activated macrophages, as well the percentage of phagocytosis of NR by macrophages in vitro. Both Gl-SP and Gl-BSP could promote MLR, however, at the dose of 12.8 mg/L, Gl-BSP showed higher activity than Gl-SP in the proliferation of lymphocytes. These two kinds of polysaccharide could significantly increase the secretion of IL-2 and IFN-gamma in doublejway MLR at the concentrations of 0.2-12.8 mg/L, but Gl-BSP had stronger effects than Gl-SP at the same concentrations. Both Gl-SP and Gl-BSP could increase the ratio of T-lymphocyte subpopulations in double-way MLR. At the concentrations of 0.2-12.8 mg/L or 3.2-12.8 mg/L, Gl-BSP demonstrated more significant activity in increasing the percentage of the CD4(+) or CD8(+) subset than Gl-SP. At the concentrations of 0.2-0.8 mg/L, the ratio of the CD4(+) and CD8(+) subset in the Gl-BSP treated group was higher than that of the Gl-SP treated group.
    Conclusion: Gl-SP and Gl-BSP have similar immunomodulatory effects in vitro, as though the immunomodulatory effects of Gl-BSP are stronger than that of Gl-SP.
    MeSH term(s) Adjuvants, Immunologic/pharmacology ; Animals ; Cell Survival/drug effects ; Cell Survival/immunology ; Enzyme-Linked Immunosorbent Assay ; Female ; Flow Cytometry ; Interferon-gamma/analysis ; Interleukin-2/analysis ; Lymphocytes/drug effects ; Lymphocytes/immunology ; Lymphocytes/metabolism ; Macrophages, Peritoneal/drug effects ; Macrophages, Peritoneal/immunology ; Macrophages, Peritoneal/metabolism ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Polysaccharides/isolation & purification ; Polysaccharides/pharmacology ; Reishi/chemistry ; Spleen/cytology ; Spores, Fungal/chemistry ; T-Lymphocytes, Cytotoxic/drug effects ; T-Lymphocytes, Cytotoxic/immunology ; Tumor Necrosis Factor-alpha/analysis
    Chemical Substances Adjuvants, Immunologic ; Interleukin-2 ; Polysaccharides ; Tumor Necrosis Factor-alpha ; Interferon-gamma (82115-62-6)
    Language Chinese
    Publishing date 2005-12-18
    Publishing country China
    Document type Comparative Study ; English Abstract ; Journal Article
    ISSN 1671-167X
    ISSN 1671-167X
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article: Ganoderma total sterol (GS) and GS1 protect rat cerebral cortical neurons from hypoxia/reoxygenation injury.

    Zhao, Hong-Bo / Wang, Sai-Zhen / He, Qi-Hua / Yuan, Lan / Chen, Alex F / Lin, Zhi-Bin

    Life sciences

    2005  Volume 76, Issue 9, Page(s) 1027–1037

    Abstract: The effect of Ganoderma total sterol (GS) and its main components(GS(1)) on rat cortical neuronal cultures exposed to hypoxia/reoxygenation (H/R) was studied in vitro. GS (0.01,0.1,1 microg/ml) increased neuron viability following H/R. GS also ... ...

    Abstract The effect of Ganoderma total sterol (GS) and its main components(GS(1)) on rat cortical neuronal cultures exposed to hypoxia/reoxygenation (H/R) was studied in vitro. GS (0.01,0.1,1 microg/ml) increased neuron viability following H/R. GS also significantly reduced malondialdehyde content and reactive oxygen species production and increased manganese superoxide dismutase (Mn-SOD) activity; furthermore, the translocation of nuclear factor-kappa B and the production of interleukin-1beta and tumor necrosis factor alpha induced by H/R were also blocked. These findings suggest that GS might be useful in treating H/R-induced oxidative stress and inflammatory response. We also hypothesized that Mn-SOD might play a critical role in the neuroprotective effect of GS against H/R injury. In addition, pretreatment with GS(1) (0.01, 0.1, 1 microg/ml) significantly attenuated the decline of neuron viability and the formation of reactive oxygen species. Furthermore GS(1) possessed more potent protective effect on neurons compared with GS at the same dose. These findings demonstrated that GS(1) is the main component in GS; and play a critical role in the neuroprotective effect of GS against H/R.
    MeSH term(s) Animals ; Cell Hypoxia/drug effects ; Cell Survival/drug effects ; Cells, Cultured ; Cerebral Cortex/drug effects ; Cerebral Cortex/metabolism ; Cholestadienols/pharmacology ; Cytokines/biosynthesis ; Ganoderma/chemistry ; I-kappa B Proteins/metabolism ; NF-KappaB Inhibitor alpha ; NF-kappa B/metabolism ; Neuroprotective Agents/pharmacology ; Oxidative Stress/drug effects ; Phytosterols/pharmacology ; Rats ; Rats, Sprague-Dawley ; Superoxide Dismutase/metabolism
    Chemical Substances Cholestadienols ; Cytokines ; I-kappa B Proteins ; NF-kappa B ; Neuroprotective Agents ; Nfkbia protein, rat ; Phytosterols ; NF-KappaB Inhibitor alpha (139874-52-5) ; brassicasterol (2B0KG2XFOF) ; Superoxide Dismutase (EC 1.15.1.1)
    Language English
    Publishing date 2005-01-14
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 3378-9
    ISSN 1879-0631 ; 0024-3205
    ISSN (online) 1879-0631
    ISSN 0024-3205
    DOI 10.1016/j.lfs.2004.08.013
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Uc I Zs.368: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top