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Article ; Online: Genomic Characterization and Molecular Evolution of Sapovirus in Children under 5 Years of Age.

Ji, Xiaolei / Guo, Chen / Dai, Yaoyao / Chen, Lu / Chen, Yujia / Wang, Shifang / Sun, Yihua

2024 Volume 16, Issue 1

Abstract: Sapovirus (SaV) is a type of gastroenteric virus that can cause acute gastroenteritis. It is highly contagious, particularly among children under the age of 5. In this study, a total of 712 stool samples from children under the age of 5 with acute ... ...

Abstract	Sapovirus (SaV) is a type of gastroenteric virus that can cause acute gastroenteritis. It is highly contagious, particularly among children under the age of 5. In this study, a total of 712 stool samples from children under the age of 5 with acute gastroenteritis were collected. Out of these samples, 28 tested positive for SaV, resulting in a detection rate of 3.93% (28/712). Samples with Ct < 30 were collected for library construction and high-throughput sequencing, resulting in the acquisition of nine complete genomes. According to Blast, eight of them were identified as GI.1, while the remaining one was GI.6. The GI.6 strain sequence reported in our study represents the first submission of the GI.6 strain complete genome sequence from mainland China to the Genbank database, thus filling the data gap in our country. Sequence identity analysis revealed significant nucleotide variations between the two genotypes of SaV and their corresponding prototype strains. Phylogenetic and genetic evolution analyses showed no evidence of recombination events in the obtained sequences. Population dynamics analysis demonstrated potential competitive inhibition between two lineages of GI.1. Our study provides insights into the molecular epidemiological and genetic evolution characteristics of SaV prevalent in the Nantong region of China, laying the foundation for disease prevention and control, as well as pathogen tracing related to SaV in this area.
MeSH term(s)	Child ; Humans ; Child, Preschool ; Sapovirus/genetics ; Phylogeny ; Gastroenteritis/epidemiology ; Genotype ; Genomics ; Caliciviridae Infections/epidemiology ; Feces
Language	English
Publishing date	2024-01-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v16010146
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Enhanced Organic Contaminant Retardation by CTMAB-Modified Bentonite Backfill in Cut-Off Walls: Laboratory Test and Numerical Investigation.

He, Haijie / Wu, Tao / Shu, Xiaole / Chai, Kuan / Qiu, Zhanhong / Wang, Shifang / Yao, Jun

Materials (Basel, Switzerland)

2023 Volume 16, Issue 3

Abstract: Adding organically modified bentonite into impervious wall materials may improve the adsorption of organic pollutants. In this study, cetyltrimethylammonium bromide organically modified bentonite (CTMAB bentonite) was mixed with sodium bentonite and ... ...

Abstract	Adding organically modified bentonite into impervious wall materials may improve the adsorption of organic pollutants. In this study, cetyltrimethylammonium bromide organically modified bentonite (CTMAB bentonite) was mixed with sodium bentonite and kaolin to obtain two materials, which were then used as cut-off walls for typical pollutants. Soil column consolidation tests, diffusion tests, and breakdown tests were conducted to study migration of organic pollutants in soil columns. The parameter sensitivity of pollutant transport in the cut-off wall was analysed by numerical simulation. The sodium bentonite mixed with 10% CTMAB bentonite and kaolin-CTMAB bentonite showed the greatest impermeability: with a consolidation pressure of 200 kPa, the permeability coefficients were 1.03 × 10
Language	English
Publishing date	2023-02-01
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2487261-1
ISSN	1996-1944
ISSN	1996-1944
DOI	10.3390/ma16031255
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Article ; Online: An Apparent Gas Permeability Model for Real Gas Flow in Fractured Porous Media with Roughened Surfaces.

Wu, Tao / Wang, Qian / Wang, Shifang

Polymers

2021 Volume 13, Issue 12

Abstract: The investigation of gas transport in fractured porous media is essential in most petroleum and chemical engineering. In this paper, an apparent gas permeability model for real gas flow in fractured porous media is derived with adequate consideration of ... ...

Abstract	The investigation of gas transport in fractured porous media is essential in most petroleum and chemical engineering. In this paper, an apparent gas permeability model for real gas flow in fractured porous media is derived with adequate consideration of real gas effect, the roughness of fracture surface, and Knudsen diffusion based on the fractal theory. The fractal apparent gas permeability model is obtained to be a function of micro-structural parameters of fractured porous media, relative roughness, the pressure, the temperature, and the properties of gas. The predictions from the apparent gas permeability model based on the fractal theory match well with the published permeability model and experimental data, which verifies the rationality of the present fractal apparent gas permeability model.
Language	English
Publishing date	2021-06-10
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2527146-5
ISSN	2073-4360 ; 2073-4360
ISSN (online)	2073-4360
ISSN	2073-4360
DOI	10.3390/polym13121937
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Article: [Clinical Characteristics and Survival Analysis of Patients with Chronic Myelomonocytic Leukemia].

Wang, Jing-Yu / Chen, Jian-Fang / Wang, Shi-Fang / Guo, Qiao-Hua / Ma, Yan-Ping

Zhongguo shi yan xue ye xue za zhi

2023 Volume 31, Issue 2, Page(s) 476–482

Abstract: Objective: To investigate the clinical characteristics, prognostic factors and efficacy of hypomethylating agent (HMA) in patients with chronic myelomonocytic leukemia (CMML).: Methods: The clinical data of 37 newly diagnosed patients with CMML was ... ...

Abstract	Objective: To investigate the clinical characteristics, prognostic factors and efficacy of hypomethylating agent (HMA) in patients with chronic myelomonocytic leukemia (CMML). Methods: The clinical data of 37 newly diagnosed patients with CMML was analyzed retrospectively, and their clinical characteristics and the efficacy of HMA were summarized. Kaplan-Meier and Log-rank test were used for univariate survival analysis, and Cox proportional hazards regression model was used for multivariate analysis. Results: The median age at diagnosis was 67 years old. Their common manifestations included fatigue, bleeding, abnormal blood routine and fever. Most patients had splenomegaly. According to FAB classification, there were 6 cases of myelodysplastic CMML and 31 cases of myeloproliferative CMML, while according to WHO classification, 8 patients belonged to CMML-0, 9 patients to CMML-1 and 20 patients to CMML-2. At the time of diagnosis, the median white blood cell count was 32.84×10 Conclusion: CMML has high heterogeneity in clinical characteristics, genetic changes, prognosis and treatment response. HMA can not significantly improve the survival of CMML patients. ANC≥12×10
MeSH term(s)	Humans ; Aged ; Leukemia, Myelomonocytic, Chronic/genetics ; Retrospective Studies ; In Situ Hybridization, Fluorescence ; Survival Analysis ; Prognosis
Language	Chinese
Publishing date	2023-04-25
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	2404306-0
ISSN	1009-2137
ISSN	1009-2137
DOI	10.19746/j.cnki.issn.1009-2137.2023.02.024
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Article: [Clinical Characteristics and Survival Analysis of Patients with Plasma Cell Leukemia].

Wang, Shi-Fang / Qin, Xiao-Qi / Guo, Qiao-Hua / Wang, Jing-Yu / Ma, Yan-Ping

Zhongguo shi yan xue ye xue za zhi

2023 Volume 31, Issue 3, Page(s) 693–698

Abstract: Objective: To investigate the clinical characteristics, therapeutic response and prognosis of patients with plasma cell leukemia (PCL) and improve the understanding of this disease.: Methods: The clinical manifestations, laboratory tests and ... ...

Abstract	Objective: To investigate the clinical characteristics, therapeutic response and prognosis of patients with plasma cell leukemia (PCL) and improve the understanding of this disease. Methods: The clinical manifestations, laboratory tests and treatment response of 27 patients with plasma cell leukemia treated in The Second Hospital of Shanxi Medical University from December 2010 to August 2019 were analyzed retrospectively, and their clinical characteristics were summarized. Kaplan-Meier method was used for survival analysis. Results: There were 18 cases of primary plasma cell leukemia (pPCL) and 9 cases of secondary plasma cell leukemia (sPCL). The male to female ratio was 1.7∶1. The median age was 62 years old. The first manifestations were bone pain, fatigue, fever, splenomegaly and bleeding, and a large number of plasma cell infiltration was observed in the morphological examination of peripheral blood and bone marrow cells. 13 cases were detected by immunotyping and all of them expressed CD38/CD138. 8 cases underwent karyotype analysis, and 3 cases were normal, clonal abnormalities occurred in 5 cases. FISH detection was performed in 12 cases, of which 8 cases were abnormal. In 17 cases of bortezomib based chemotherapy, the ovevall response rate was 52.9%, which was higher than that in the non-bortezomib group, but there was no significant difference between the two groups ( Conclusion: PCL is highly invasive and has diverse clinical manifestations, and is not sensitive to traditional chemotherapy. The median survival time of patients with pPCL is relatively longer than that of patients with sPCL. The chemotherapy regimen based on bortezomib improves the treatment effectiveness and prolongs the survival time of PCL patients.
MeSH term(s)	Male ; Female ; Humans ; Leukemia, Plasma Cell/diagnosis ; Retrospective Studies ; Bortezomib/therapeutic use ; Prognosis ; Survival Analysis
Chemical Substances	Bortezomib (69G8BD63PP)
Language	Chinese
Publishing date	2023-03-11
Publishing country	China
Document type	English Abstract ; Journal Article
ZDB-ID	2404306-0
ISSN	1009-2137
ISSN	1009-2137
DOI	10.19746/j.cnki.issn.1009-2137.2023.03.012
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Article: Copper-Catalyzed Ring-Opening Defluorinative Alkylation of Siloxydifluorocyclopropanes: Synthesis of γ-Fluoro-δ-Ketoesters and γ,δ-Diketonitriles

Wang, Shifang / Liu, Xiaowei / Zhu, Dongsheng / Wang, Mang

Journal of organic chemistry. 2020 Sept. 04, v. 85, no. 19

2020

Abstract: In view of the importance of both fluorinated synthons and homoenolate equivalents, synthetic application of difluorocyclopropanols is desired but remains challenging due to their thermodynamic instability. Herein, we use siloxydifluorocyclopropanes as ... ...

Abstract	In view of the importance of both fluorinated synthons and homoenolate equivalents, synthetic application of difluorocyclopropanols is desired but remains challenging due to their thermodynamic instability. Herein, we use siloxydifluorocyclopropanes as difluorocyclopropanol precursors to carry out new Cu-catalyzed ring-opening defluorinative alkylation. With α-bromo carboxylic esters as coupling partners, the reaction affords γ-fluoro-δ-ketoesters via a Cuᴵ/Cuᴵᴵ catalytic cycle. Interestingly, by the use of α-bromoamides, the ring-opening defluorinative alkylation is followed by an additional intramolecular C–N oxidative coupling to deliver a lactam intermediate, which further undergoes defluorination, hydrolysis, ring opening, and dehydration cascade via a Cuᴵ/Cuᴵᴵ/Cuᴵᴵᴵ catalytic pathway, leading to γ,δ-diketonitriles as the final products.
Keywords	alkylation ; hydrolysis ; lactams ; organic chemistry ; thermodynamics
Language	English
Dates of publication	2020-0904
Size	p. 12408-12417.
Publishing place	American Chemical Society
Document type	Article
ZDB-ID	123490-0
ISSN	1520-6904 ; 0022-3263
ISSN (online)	1520-6904
ISSN	0022-3263
DOI	10.1021/acs.joc.0c01643
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Article ; Online: Development of Systemic Lupus Erythematosus After Infectious Mononucleosis in a 64-Year-Old Woman.

Wang, Shifang / Wang, Shiyu / Singh, Shaleindra

Journal of investigative medicine high impact case reports

2020 Volume 8, Page(s) 2324709620961613

Abstract: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by heterogeneous symptoms that can manifest in any organ, and often presents at a young age. Infectious mononucleosis (IM) is the acute clinical manifestation of Epstein- ... ...

Abstract	Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by heterogeneous symptoms that can manifest in any organ, and often presents at a young age. Infectious mononucleosis (IM) is the acute clinical manifestation of Epstein-Barr virus (EBV). It is characterized by low-grade fever, malaise, lymphadenopathy, splenomegaly, and occasionally symmetrical arthralgias. It has been proposed that EBV is a trigger for new-onset SLE, and patients with autoimmune disorders such as SLE are more likely to have recurrent IM infections. The patient, a 64-year old Caucasian female who's only past medical history was hypertension, developed several months-long period of vague symptoms, including fatigue, malaise, nausea, and nonbilious vomiting with oral intake. She presented with symmetrical polyarthritis involving the hands and elbows, with no history of arthritis before this episode. At the 5-month follow-up, she presented with worsening arthritis bilaterally in her elbows and in her right knee. For several decades, there has been a theoretical association between EBV and SLE, with EBV thought to be one of the many possible triggers for development of SLE. Based on the disease course, we theorize that the patient's IM and EBV infection led to development of SLE. A small fraction of SLE cases have been reported in literature to be associated with EBV. This case adds to that literature with EBV triggering development of SLE in a seemingly previously asymptomatic patient.
MeSH term(s)	Antibodies, Viral/analysis ; Female ; Herpesvirus 4, Human/immunology ; Humans ; Infectious Mononucleosis/complications ; Infectious Mononucleosis/diagnosis ; Lupus Erythematosus, Systemic/diagnosis ; Middle Aged
Chemical Substances	Antibodies, Viral
Language	English
Publishing date	2020-09-23
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	2710326-2
ISSN	2324-7096 ; 2324-7096
ISSN (online)	2324-7096
ISSN	2324-7096
DOI	10.1177/2324709620961613
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Article ; Online: Copper-Catalyzed Ring-Opening Defluorinative Alkylation of Siloxydifluorocyclopropanes: Synthesis of γ-Fluoro-δ-Ketoesters and γ,δ-Diketonitriles.

Wang, Shifang / Liu, Xiaowei / Zhu, Dongsheng / Wang, Mang

The Journal of organic chemistry

2020 Volume 85, Issue 19, Page(s) 12408–12417

Abstract	In view of the importance of both fluorinated synthons and homoenolate equivalents, synthetic application of difluorocyclopropanols is desired but remains challenging due to their thermodynamic instability. Herein, we use siloxydifluorocyclopropanes as difluorocyclopropanol precursors to carry out new Cu-catalyzed ring-opening defluorinative alkylation. With α-bromo carboxylic esters as coupling partners, the reaction affords γ-fluoro-δ-ketoesters
Language	English
Publishing date	2020-09-16
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	123490-0
ISSN	1520-6904 ; 0022-3263
ISSN (online)	1520-6904
ISSN	0022-3263
DOI	10.1021/acs.joc.0c01643
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Article: Adsorption of Rhodamine B from Simulated Waste Water onto Kaolin-Bentonite Composites.

He, Haijie / Chai, Kuan / Wu, Tao / Qiu, Zhanhong / Wang, Shifang / Hong, Jie

Materials (Basel, Switzerland)

2022 Volume 15, Issue 12

Abstract: Organic dye rhodamine B is one of the common organic pollutants in the water and soil environment. This study investigated the feasibility of removing rhodamine B from an aqueous solution through adsorption by kaolin, kaolin-sodium bentonite, and kaolin- ... ...

Abstract	Organic dye rhodamine B is one of the common organic pollutants in the water and soil environment. This study investigated the feasibility of removing rhodamine B from an aqueous solution through adsorption by kaolin, kaolin-sodium bentonite, and kaolin-organic bentonite. Batch adsorption test results showed that the maximum adsorption quantities of kaolin, kaolin-sodium bentonite, and kaolin-organic bentonite were 7.76 mg/g, 11.26 mg/g, and 12.68 mg/g, respectively, implying that the addition of bentonite to kaolin can effectively improve its adsorption capacity for rhodamine B. Moreover, the Langmuir isotherm model is suitable to describe the adsorption of rhodamine B by kaolin and kaolin-sodium bentonite, while it is preferable to use the Freundlich isotherm model in the case of kaolin-organic bentonite. The adsorption kinetic characteristics of rhodamine B, by these three adsorbents, are suitable to be described with a pseudo-second order kinetic model. Furthermore, the characteristics of the above three adsorbents were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The above results indicated that kaolin and organic bentonite can be used to design efficient adsorbents for organic pollutants similar to rhodamine B.
Language	English
Publishing date	2022-06-07
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2487261-1
ISSN	1996-1944
ISSN	1996-1944
DOI	10.3390/ma15124058
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Article ; Online: Determination of the median effective dose of sufentanil for inhibiting the laryngeal mask insertion response in geriatric patients: a prospective, double-blinded, dose-response trial.

Wang, ShiFang / Wang, WeiBing / Xiao, JinBo / Yu, HongPing / Zhou, Hui / Xu, Huang

BMC anesthesiology

2022 Volume 22, Issue 1, Page(s) 216

Abstract: Background: Laryngeal mask airway(LMA) have been widely used in clinical practice. Irritation to the patient during the insertion of a laryngeal mask can cause hemodynamic fluctuations, which is particularly unsafe for geriatric patients. We used probit ...

Abstract	Background: Laryngeal mask airway(LMA) have been widely used in clinical practice. Irritation to the patient during the insertion of a laryngeal mask can cause hemodynamic fluctuations, which is particularly unsafe for geriatric patients. We used probit regression analysis to determine the median effective dose of sufentanil to inhibit the response to LMA insertion in geriatric patients. Methods: A total of 90 patients were selected for the study using the following inclusion criteria: age ≥ 65 years old, ASA grade I-III, and scheduled to undergo intravenous general anesthesia with LMA insertion. Each patient received a dose of sufentanil for anesthesia induction in one of six levels: 0.05, 0.1, 0.15, 0.2, 0.25, or 0.3 μg kg Results: Probit regression analysis showed that the ED50 of sufentanil inhibiting the response to LMA insertion in geriatric patients was 0.18 μg kg Conclusions: Sufentanil can effectively inhibit the patient's response to LMA insertion, with stable hemodynamics and small stress response. The ED50 and ED95 were 0.18 μg kg Trial registration: This study was registered in the Chinese Clinical Trial Registry (Registration number: ChiCTR2100051827 ) on October 6, 2021.
MeSH term(s)	Aged ; Anesthesia, General ; Anesthesia, Intravenous ; Double-Blind Method ; Humans ; Laryngeal Masks ; Prospective Studies ; Sufentanil
Chemical Substances	Sufentanil (AFE2YW0IIZ)
Language	English
Publishing date	2022-07-11
Publishing country	England
Document type	Clinical Trial ; Journal Article
ZDB-ID	2091252-3
ISSN	1471-2253 ; 1471-2253
ISSN (online)	1471-2253
ISSN	1471-2253
DOI	10.1186/s12871-022-01758-7
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