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  1. Article ; Online: Protein detection in blood with single-molecule imaging.

    Mao, Chih-Ping / Wang, Shih-Chin / Su, Yu-Pin / Tseng, Ssu-Hsueh / He, Liangmei / Wu, Annie A / Roden, Richard B S / Xiao, Jie / Hung, Chien-Fu

    Science advances

    2021  Volume 7, Issue 33

    Abstract: The ability to characterize individual biomarker protein molecules in patient blood samples could enable diagnosis of diseases at an earlier stage, when treatment is typically more effective. Single-molecule imaging offers a promising approach to ... ...

    Abstract The ability to characterize individual biomarker protein molecules in patient blood samples could enable diagnosis of diseases at an earlier stage, when treatment is typically more effective. Single-molecule imaging offers a promising approach to accomplish this goal. However, thus far, single-molecule imaging methods have not been translated into the clinical setting. The detection limit of these methods has been confined to the picomolar (10
    MeSH term(s) Biomarkers/analysis ; Humans ; Nanotechnology ; Proteins/analysis ; Single Molecule Imaging/methods
    Chemical Substances Biomarkers ; Proteins
    Language English
    Publishing date 2021-08-11
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2810933-8
    ISSN 2375-2548 ; 2375-2548
    ISSN (online) 2375-2548
    ISSN 2375-2548
    DOI 10.1126/sciadv.abg6522
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Development and application of a high-content virion display human GPCR array.

    Syu, Guan-Da / Wang, Shih-Chin / Ma, Guangzhong / Liu, Shuang / Pearce, Donna / Prakash, Atish / Henson, Brandon / Weng, Lien-Chun / Ghosh, Devlina / Ramos, Pedro / Eichinger, Daniel / Pino, Ignacio / Dong, Xinzhong / Xiao, Jie / Wang, Shaopeng / Tao, Nongjian / Kim, Kwang Sik / Desai, Prashant J / Zhu, Heng

    Nature communications

    2019  Volume 10, Issue 1, Page(s) 1997

    Abstract: Human G protein-coupled receptors (GPCRs) respond to various ligands and stimuli. However, GPCRs rely on membrane for proper folding, making their biochemical properties difficult to study. By displaying GPCRs in viral envelopes, we fabricated a Virion ... ...

    Abstract Human G protein-coupled receptors (GPCRs) respond to various ligands and stimuli. However, GPCRs rely on membrane for proper folding, making their biochemical properties difficult to study. By displaying GPCRs in viral envelopes, we fabricated a Virion Display (VirD) array containing 315 non-olfactory human GPCRs for functional characterization. Using this array, we found that 10 of 20 anti-GPCR mAbs were ultra-specific. We further demonstrated that those failed in the mAb assays could recognize their canonical ligands, suggesting proper folding. Next, using two peptide ligands on the VirD-GPCR array, we identified expected interactions and novel interactions. Finally, we screened the array with group B Streptococcus, a major cause of neonatal meningitis, and demonstrated that inhibition of a newly identified target, CysLTR1, reduced bacterial penetration both in vitro and in vivo. We believe that the VirD-GPCR array holds great potential for high-throughput screening for small molecule drugs, affinity reagents, and ligand deorphanization.
    MeSH term(s) Animals ; Blotting, Western ; Chlorocebus aethiops ; Fluorescent Antibody Technique ; HEK293 Cells ; HeLa Cells ; Humans ; Proteomics/methods ; Receptors, G-Protein-Coupled/metabolism ; Streptococcus/metabolism ; Vero Cells ; Virion/metabolism ; Virology/methods
    Chemical Substances Receptors, G-Protein-Coupled
    Language English
    Publishing date 2019-04-30
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-019-09938-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Preoperative transarterial chemoembolization and resection for hepatocellular carcinoma: a nationwide Taiwan database analysis of long-term outcome predictors.

    Shi, Hon-Yi / Wang, Shen-Nien / Wang, Shih Chin / Chuang, Shih-Chang / Chen, Chun-Min / Lee, King-Teh

    Journal of surgical oncology

    2014  Volume 109, Issue 5, Page(s) 487–493

    Abstract: Background: To explore long-term predictors of outcome after TACE and resection in a population of patients with hepatocellular carcinoma (HCC).: Methods: A total of 648 had received TACE before liver resection (TACE group) while 10,431 patients had ... ...

    Abstract Background: To explore long-term predictors of outcome after TACE and resection in a population of patients with hepatocellular carcinoma (HCC).
    Methods: A total of 648 had received TACE before liver resection (TACE group) while 10,431 patients had received liver resection without TACE (LR group). Propensity scores were calculated by entering the patient data into a logistic regression model for predicting HCC outcomes.
    Results: Compared to the LR group, the TACE group did not significantly differ in disease-free survival (DFS) (median, 17 months in the TACE group vs. 13 months in the LR group; P = 0.410) and overall-survival (OS) (median, 56 months in the TACE group vs. 54 months in the LR group; P = 0.777). The TACE group also showed that gender, liver cirrhosis, CCI score, hospital volume, and surgeon volume were independently associated with DFS while gender, CCI score and hospital level were independently associated with DFS/OS.
    Conclusions: This population-based cohort study provides compelling evidence that preoperative TACE does not significantly reduce DFS or OS in patients with resectable HCC. Moreover, long-term outcomes for these procedures are significantly associated with patient characteristics and hospital characteristics. Medical professionals and health care providers should carefully evaluate candidates for preoperative TACE in patients with resectable HCC.
    MeSH term(s) Adult ; Aged ; Carcinoma, Hepatocellular/drug therapy ; Carcinoma, Hepatocellular/surgery ; Chemoembolization, Therapeutic/methods ; Chemoembolization, Therapeutic/statistics & numerical data ; Confounding Factors (Epidemiology) ; Databases, Factual ; Disease-Free Survival ; Female ; Hepatectomy/statistics & numerical data ; Hepatic Artery ; Hepatitis B/complications ; Hepatitis C/complications ; Hospitals/statistics & numerical data ; Humans ; Kaplan-Meier Estimate ; Liver Neoplasms/drug therapy ; Liver Neoplasms/surgery ; Male ; Middle Aged ; Neoadjuvant Therapy/methods ; Predictive Value of Tests ; Prognosis ; Propensity Score ; Proportional Hazards Models ; Retrospective Studies ; Risk Factors ; Taiwan ; Treatment Outcome
    Language English
    Publishing date 2014-04
    Publishing country United States
    Document type Journal Article
    ZDB-ID 82063-5
    ISSN 1096-9098 ; 0022-4790
    ISSN (online) 1096-9098
    ISSN 0022-4790
    DOI 10.1002/jso.23521
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 706: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
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  4. Article ; Online: In vivo organization of the FtsZ-ring by ZapA and ZapB revealed by quantitative super-resolution microscopy.

    Buss, Jackson / Coltharp, Carla / Huang, Tao / Pohlmeyer, Chris / Wang, Shih-Chin / Hatem, Christine / Xiao, Jie

    Molecular microbiology

    2013  Volume 89, Issue 6, Page(s) 1099–1120

    Abstract: In most bacterial cells, cell division is dependent on the polymerization of the FtsZ protein to form a ring-like structure (Z-ring) at the midcell. Despite its essential role, the molecular architecture of the Z-ring remains elusive. In this work we ... ...

    Abstract In most bacterial cells, cell division is dependent on the polymerization of the FtsZ protein to form a ring-like structure (Z-ring) at the midcell. Despite its essential role, the molecular architecture of the Z-ring remains elusive. In this work we examine the roles of two FtsZ-associated proteins, ZapA and ZapB, in the assembly dynamics and structure of the Z-ring in Escherichia coli cells. In cells deleted of zapA or zapB, we observed abnormal septa and highly dynamic FtsZ structures. While details of these FtsZ structures are difficult to discern under conventional fluorescence microscopy, single-molecule-based super-resolution imaging method Photoactivated Localization Microscopy (PALM) reveals that these FtsZ structures arise from disordered arrangements of FtsZ clusters. Quantitative analysis finds these clusters are larger and comprise more molecules than a single FtsZ protofilament, and likely represent a distinct polymeric species that is inherent to the assembly pathway of the Z-ring. Furthermore, we find these clusters are not due to the loss of ZapB-MatP interaction in ΔzapA and ΔzapB cells. Our results suggest that the main function of ZapA and ZapB in vivo may not be to promote the association of individual protofilaments but to align FtsZ clusters that consist of multiple FtsZ protofilaments.
    MeSH term(s) Bacterial Proteins/metabolism ; Carrier Proteins/genetics ; Carrier Proteins/metabolism ; Cell Cycle Proteins/genetics ; Cell Cycle Proteins/metabolism ; Cytoskeletal Proteins/metabolism ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Escherichia coli Proteins/genetics ; Escherichia coli Proteins/metabolism ; Gene Deletion ; Macromolecular Substances/metabolism ; Microscopy/methods ; Protein Multimerization
    Chemical Substances Bacterial Proteins ; Carrier Proteins ; Cell Cycle Proteins ; Cytoskeletal Proteins ; Escherichia coli Proteins ; FtsZ protein, Bacteria ; Macromolecular Substances ; ZapA protein, E coli ; ZapB protein, E coli
    Language English
    Publishing date 2013-08-14
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 619315-8
    ISSN 1365-2958 ; 0950-382X
    ISSN (online) 1365-2958
    ISSN 0950-382X
    DOI 10.1111/mmi.12331
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2502: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  5. Article ; Online: Aqueous Extract of Paeonia suffruticosa Inhibits Migration and Metastasis of Renal Cell Carcinoma Cells via Suppressing VEGFR-3 Pathway.

    Wang, Shih-Chin / Tang, Sai-Wen / Lam, Sio-Hong / Wang, Chung-Chieh / Liu, Yu-Huei / Lin, Hsuan-Yuan / Lee, Shoei-Sheng / Lin, Jung-Yaw

    Evidence-based complementary and alternative medicine : eCAM

    2012  Volume 2012, Page(s) 409823

    Abstract: Renal cell carcinoma (RCC) cells are characterized by strong drug resistance and high metastatic incidence. In this study, the effects of ten kinds of Chinese herbs on RCC cell migration and proliferation were examined. Aqueous extract of Paeonia ... ...

    Abstract Renal cell carcinoma (RCC) cells are characterized by strong drug resistance and high metastatic incidence. In this study, the effects of ten kinds of Chinese herbs on RCC cell migration and proliferation were examined. Aqueous extract of Paeonia suffruticosa (PS-A) exerted strong inhibitory effects on cancer cell migration, mobility, and invasion. The results of mouse xenograft experiments showed that the treatment of PS-A significantly suppressed tumor growth and pulmonary metastasis. We further found that PS-A markedly decreased expression of VEGF receptor-3 (VEGFR-3) and phosphorylation of FAK in RCC cells. Moreover, the activation of Rac-1, a modulator of cytoskeletal dynamics, was remarkably reduced by PS-A. Additionally, PS-A suppressed polymerization of actin filament as demonstrated by confocal microscopy analysis and decreased the ratio of F-actin to G-actin in RCC cells, suggesting that PS-A inhibits RCC cell migration through modulating VEGFR-3/FAK/Rac-1 pathway to disrupt actin filament polymerization. In conclusion, this research elucidates the effects and molecular mechanism for antimigration of PS-A on RCC cells and suggests PS-A to be a therapeutic or adjuvant strategy for the patients with aggressive RCC.
    Language English
    Publishing date 2012-02-08
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2171158-6
    ISSN 1741-4288 ; 1741-427X
    ISSN (online) 1741-4288
    ISSN 1741-427X
    DOI 10.1155/2012/409823
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    Zs.A 5995: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  6. Article ; Online: Optomotor-blind negatively regulates Drosophila eye development by blocking Jak/STAT signaling.

    Tsai, Yu-Chen / Grimm, Stefan / Chao, Ju-Lan / Wang, Shih-Chin / Hofmeyer, Kerstin / Shen, Jie / Eichinger, Fred / Michalopoulou, Theoni / Yao, Chi-Kuang / Chang, Chih-Hsuan / Lin, Shih-Han / Sun, Y Henry / Pflugfelder, Gert O

    PloS one

    2015  Volume 10, Issue 3, Page(s) e0120236

    Abstract: Organ formation requires a delicate balance of positive and negative regulators. In Drosophila eye development, wingless (wg) is expressed at the lateral margins of the eye disc and serves to block retinal development. The T-box gene optomotor-blind (omb) ...

    Abstract Organ formation requires a delicate balance of positive and negative regulators. In Drosophila eye development, wingless (wg) is expressed at the lateral margins of the eye disc and serves to block retinal development. The T-box gene optomotor-blind (omb) is expressed in a similar pattern and is regulated by Wg. Omb mediates part of Wg activity in blocking eye development. Omb exerts its function primarily by blocking cell proliferation. These effects occur predominantly in the ventral margin. Our results suggest that the primary effect of Omb is the blocking of Jak/STAT signaling by repressing transcription of upd which encodes the Jak receptor ligand Unpaired.
    MeSH term(s) Animals ; Cell Proliferation/physiology ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Drosophila melanogaster ; Eye/cytology ; Eye/embryology ; Janus Kinases/genetics ; Janus Kinases/metabolism ; Nerve Tissue Proteins/genetics ; Nerve Tissue Proteins/metabolism ; STAT Transcription Factors/genetics ; STAT Transcription Factors/metabolism ; T-Box Domain Proteins/genetics ; T-Box Domain Proteins/metabolism ; Transcription Factors/genetics ; Transcription Factors/metabolism
    Chemical Substances Drosophila Proteins ; Nerve Tissue Proteins ; STAT Transcription Factors ; T-Box Domain Proteins ; Transcription Factors ; bi protein, Drosophila (146635-31-6) ; Janus Kinases (EC 2.7.10.2) ; hop protein, Drosophila (EC 2.7.10.2)
    Language English
    Publishing date 2015-03-17
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0120236
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