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Article ; Online: How to Create a New Digital Culture Environment for Teenagers in Poor Rural Areas Under the Background of Rural Revitalization

WANG Sufang, TAN Qingan

Nongye tushu qingbao xuebao, Vol 34, Iss 1, Pp 16-

Based on a Study on Internet Use Behavior and Library Services Needs of Left-Behind Children

2022 Volume 37

Abstract: Purpose/Significance] How such public culture service institutions as libraries create a better digital culture environment for children in poor rural areas under the background of digital villages is worth our attention. [Method/Process] The Internet ... ...

Abstract	[Purpose/Significance] How such public culture service institutions as libraries create a better digital culture environment for children in poor rural areas under the background of digital villages is worth our attention. [Method/Process] The Internet usage behavior and needs and expectations for library services of left-behind children were examined through mixed methods of questionnaire, focus group interviews and one-on-one interviews on the middle school students, teachers and parents in Longshan County, Xiangxi Tujia and Miao Autonomous Prefecture, Hunan Province. [Results/Conclusions] It was found that rural children preferred to use the Internet for entertainment when they were online. Internet use behavior had little to do with left-behind experience, but was significantly related to gender, education of guardians, and surrounding culture environment. The poor rural region is seriously lack of a good digital cultural environment and social support systems for teenagers. Public cultural institutions, such as school libraries and public libraries should improve their functions and services, especially digital culture services according to the needs and behavioral characteristics of teenagers.
Keywords	rural left-behind children\|internet use\|internet addiction\|library service needs\|mixed methods ; Bibliography. Library science. Information resources ; Z ; Agriculture ; S
Subject code	306
Language	Chinese
Publishing date	2022-01-01T00:00:00Z
Publisher	Editorial Department of Journal of Library and Information Science in Agriculture
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: ATAC-DEA: A Web-Based ATAC-Seq Data Differential Peak and Annotation Analysis Application.

Zhang, Shilong / Wang, Sufang

Journal of computational biology : a journal of computational molecular cell biology

2023 Volume 30, Issue 3, Page(s) 337–345

Abstract: Assay for transposase-accessible chromatin sequencing (ATAC-seq) has become one of the most widely used sequencing methods in studies of gene regulation, aiming to identify open chromatin sites and decipher how chromatin accessibility regulates gene ... ...

Abstract	Assay for transposase-accessible chromatin sequencing (ATAC-seq) has become one of the most widely used sequencing methods in studies of gene regulation, aiming to identify open chromatin sites and decipher how chromatin accessibility regulates gene expression. However, due to a lack of programming experience or minimal bioinformatics training, it is difficult for biologists to fully explore and interpret ATAC-seq results. Despite several available programs or websites that allow researchers to analyze and visualize ATAC-seq data, several limitations exist. ATAC-seq data differential expression analysis (ATAC-DEA), a web application that facilitates the exploration and visualization of differential peak analysis and annotation from ATAC-seq data, was developed (www.atac-dea.xyz:3838/ATAC-DEA). ATAC-DEA uses DiffBind and ChIPpeakAnno to process differential peak and annotation analysis results. ATAC-DEA has five features: (1) runs on a web server; (2) processes three files into one small file, which is used as the input for ATAC-DEA; (3) availability of various downloadable plots; (4) multifactor analysis and customized contrast model; and (5) annotates individual, overlapped, and differential peaks. It provides an easy-to-use user interface (UI) design for users to explore the data and modify the parameters interactively based on experimental purposes. ATAC-DEA allows biologists to generate user-friendly visual results from ATAC-seq downstream analysis.
MeSH term(s)	Chromatin Immunoprecipitation Sequencing ; High-Throughput Nucleotide Sequencing/methods ; Chromatin/genetics ; Software ; Internet ; Sequence Analysis, DNA/methods
Chemical Substances	Chromatin
Language	English
Publishing date	2023-01-19
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2030900-4
ISSN	1557-8666 ; 1066-5277
ISSN (online)	1557-8666
ISSN	1066-5277
DOI	10.1089/cmb.2022.0033
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Article: A review of Ribosome profiling and tools used in Ribo-seq data analysis.

Limbu, Mingso Sherma / Xiong, Tianze / Wang, Sufang

Computational and structural biotechnology journal

2024 Volume 23, Page(s) 1912–1918

Abstract: Translational regulation plays the most critical role in gene expression. Ribosome profiling sequencing (Ribo-Seq) is one of the methods to study translation and its regulation. It is a high throughput technology based on deep sequencing, which targets ... ...

Abstract	Translational regulation plays the most critical role in gene expression. Ribosome profiling sequencing (Ribo-Seq) is one of the methods to study translation and its regulation. It is a high throughput technology based on deep sequencing, which targets ribosome protected mRNA fragments to produce a 'global snapshot' of translatome. There has been an annual increase in the number of publications incorporating Ribo-seq technology. Because of its importance, we used PubMed database to conduct a comprehensive bibliometric analysis on Ribo-seq. We identified 2744 published articles that utilized the term 'Ribo-seq' between 2009 and Jan 2024, and 684 articles that contained both Ribo-seq and RNA-seq terms. Based on keywords correlation analysis, we discovered that the primary focus of Ribo-seq articles lies in the areas of translation, transcriptome, and ribosome in the past few years and other topics such as single-cell ribo-seq and crispr within two years, reflecting current areas of interests in Ribo-seq research. The Ribo-seq data analysis applications were also explored and summarized, providing a guide for researchers to choose corresponding tools for different types of analysis. Overall, we highlighted the advances made by Ribo-seq technologies, and the possibilities of utilizing machine learning models to unravel information from multi-omics data. The integration of Ribo-seq with other omics data, such as RNA-seq, is essential to understand the gene expression in complex biological systems.
Language	English
Publishing date	2024-04-22
Publishing country	Netherlands
Document type	Journal Article ; Review
ZDB-ID	2694435-2
ISSN	2001-0370
ISSN	2001-0370
DOI	10.1016/j.csbj.2024.04.051
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Article: The efficacy of febuxostat and allopurinol in the treatment of gout with hyperuricemia.

Wang, Sufang

Pakistan journal of pharmaceutical sciences

2018 Volume 31, Issue 4(Special), Page(s) 1623–1627

Abstract: The aim of this research work was to observe and analyze the efficacy of febuxostat and allopurinol in the treatment of gout with hyperuricemia. The 160 patients who has been diagnosed with gout and hyperuricemia in our hospital were selected as research ...

Abstract	The aim of this research work was to observe and analyze the efficacy of febuxostat and allopurinol in the treatment of gout with hyperuricemia. The 160 patients who has been diagnosed with gout and hyperuricemia in our hospital were selected as research objects. They were randomly divided into research group and control group, each containing 80. The control group received conventional allopurinol treatment, while the research group was treated with febuxostat. Then, the treatment efficacy was compared between the two groups. Through comparison of blood uric acid levels between the two groups after treatment, it can be known that improvement was more significant in the research group, P<0.05. The adverse reaction rate in the research group was significantly lower, P<0.05. For patients with gout and hyperuricemia, febuxostat therapy has better efficacy than that of allopurinol.
MeSH term(s)	Adult ; Aged ; Aged, 80 and over ; Allopurinol/adverse effects ; Allopurinol/therapeutic use ; Double-Blind Method ; Drug Therapy, Combination/adverse effects ; Febuxostat/adverse effects ; Febuxostat/therapeutic use ; Female ; Gout/complications ; Gout/drug therapy ; Gout Suppressants/adverse effects ; Gout Suppressants/therapeutic use ; Humans ; Hyperuricemia/complications ; Hyperuricemia/drug therapy ; Male ; Middle Aged ; Treatment Outcome ; Uric Acid/blood
Chemical Substances	Gout Suppressants ; Febuxostat (101V0R1N2E) ; Uric Acid (268B43MJ25) ; Allopurinol (63CZ7GJN5I)
Language	English
Publishing date	2018-09-10
Publishing country	Pakistan
Document type	Journal Article ; Randomized Controlled Trial
ZDB-ID	885131-1
ISSN	1011-601X
ISSN	1011-601X
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Article: Combing phosphorus-modified hydrochar and zeolite prepared from coal gangue for highly effective immobilization of heavy metals in coal-mining contaminated soil

Ge, Qilong / Tian, Qi / Hou, Rui / Wang, Sufang

Chemosphere. 2022 Mar., v. 291

2022

Abstract: Considering the adverse effects of heavy metals (HMs) on agriculture soil, in-situ immobilization has been paid great attention worldwide. P-modified biochar/hydrochar along with synthetic zeolite for efficient HMs immobilization in contaminated soil ... ...

Abstract	Considering the adverse effects of heavy metals (HMs) on agriculture soil, in-situ immobilization has been paid great attention worldwide. P-modified biochar/hydrochar along with synthetic zeolite for efficient HMs immobilization in contaminated soil becomes a promising choice. In this study, H₃PO₄-modified hydrochar (BPH) derived from banana peels, and Na-X zeolite (ZL) prepared from coal gangue was applied individually and synergistically (1%BPH, 2%BPH 1%ZL, 2%ZL, and 1%BPH+1%ZL) to remediate a farmland soil polluted by Cd, Cu, and Pb near the coal-mining area. Compared with the mono-application of these two amendments, their combination significantly improved the soil organic carbon (SOC), electric conductivity (EC), and dehydrogenase activity. Besides, the addition of 1%CLH+1%ZL remarkably reduced the Cd, Cu, and Pb bioavailability by 67.01%, 57.01%, and 78.72%, respectively, in the soil after 100 d incubation by transforming these metals to more stable forms. The order of the HMs immobilization capacity for these two amendments was as follows: Pb > Cu > Cd. Moreover, the dominated immobilization mechanism of their synergistic application was that BPH could immobilize HMs by precipitation, complexation, and π−π electron-donor-acceptor interaction. The precipitation and complexation blocked the surface pores of BPH. The sustained release of phosphorus groups and radicals was prevented. This obstacle was possibly alleviated by adding ZL. Besides, the formation of cationic bridging, the enhancement of soil properties, and the physical adsorption of these amendments were also conducive to HMs immobilization in soil. This work indicated that co-application of BPH and ZL possibly was an excellent choice for immobilizing HMs in soil.
Keywords	adsorption ; agricultural soils ; bananas ; bioavailability ; biochar ; coal ; electrical conductivity ; hydrochars ; immobilization in soil ; oxidoreductases ; phosphorus ; polluted soils ; soil organic carbon ; zeolites
Language	English
Dates of publication	2022-03
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	120089-6
ISSN	1879-1298 ; 0045-6535 ; 0366-7111
ISSN (online)	1879-1298
ISSN	0045-6535 ; 0366-7111
DOI	10.1016/j.chemosphere.2021.132835
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Article ; Online: Antibodies against Toxoplasma gondii positive in serum and aqueous humor to diagnose clinically suspected ocular toxoplasmosis: A case report.

Niu, Lixia / Wang, Sufang / Li, Yunyun / Liu, Jun

Medicine

2022 Volume 101, Issue 40, Page(s) e30956

Abstract: Introduction: Previously, diagnosis of ocular toxoplasmosis is based on clinical symptoms and Toxoplasma serology. Checking serological indicators often cannot reflect the real intraocular situation, and may even mislead clinicians to make wrong ... ...

Abstract	Introduction: Previously, diagnosis of ocular toxoplasmosis is based on clinical symptoms and Toxoplasma serology. Checking serological indicators often cannot reflect the real intraocular situation, and may even mislead clinicians to make wrong judgments. Patient concerns: A 38-year-old male patient visited our ophthalmology clinic with a chief complaint of decreased vision for about 5 days in his right eye. Diagnosis: Aqueous humor sample analysis found Toxoplasma DNA detectable, and Toxoplasma immunoglobulin G (IgG) and immunoglobulin M (IgM) to be positive. His serum Toxoplasma IgG was also positive (10.04 IU/mL; reference range: 0 to 7.2 IU/mL). Therefore, the final diagnose was ocular toxoplasmosis involving his right eye. Interventions: Oral prednisone 60 mg/day and azithromycin 0.25 g/day were started. Oral antibiotic treatment for toxoplasma was continued for 4 weeks, and prednisone followed by weekly stepwise tapering in steps of 10 mg/day. Outcomes: The BCVA and fundus of right eye remained stable after treatment at follow-up. Conclusions: This article reported a case of ocular Toxoplasma gondii infection diagnosis by serum and aqueous humor antibody tests. We provide some additional information on the T gondii infection diagnosis.
MeSH term(s)	Adult ; Anti-Bacterial Agents ; Antibodies, Protozoan/analysis ; Aqueous Humor ; Azithromycin/therapeutic use ; Humans ; Immunoglobulin G ; Immunoglobulin M ; Male ; Prednisone ; Toxoplasma ; Toxoplasmosis, Ocular/diagnosis ; Toxoplasmosis, Ocular/drug therapy
Chemical Substances	Anti-Bacterial Agents ; Antibodies, Protozoan ; Immunoglobulin G ; Immunoglobulin M ; Azithromycin (83905-01-5) ; Prednisone (VB0R961HZT)
Language	English
Publishing date	2022-10-11
Publishing country	United States
Document type	Case Reports ; Journal Article
ZDB-ID	80184-7
ISSN	1536-5964 ; 0025-7974
ISSN (online)	1536-5964
ISSN	0025-7974
DOI	10.1097/MD.0000000000030956
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Article ; Online: Bibliometric review of ATAC-Seq and its application in gene expression.

Luo, Liheng / Gribskov, Michael / Wang, Sufang

Briefings in bioinformatics

2022 Volume 23, Issue 3

Abstract: With recent advances in high-throughput next-generation sequencing, it is possible to describe the regulation and expression of genes at multiple levels. An assay for transposase-accessible chromatin using sequencing (ATAC-seq), which uses Tn5 ... ...

Abstract	With recent advances in high-throughput next-generation sequencing, it is possible to describe the regulation and expression of genes at multiple levels. An assay for transposase-accessible chromatin using sequencing (ATAC-seq), which uses Tn5 transposase to sequence protein-free binding regions of the genome, can be combined with chromatin immunoprecipitation coupled with deep sequencing (ChIP-seq) and ribonucleic acid sequencing (RNA-seq) to provide a detailed description of gene expression. Here, we reviewed the literature on ATAC-seq and described the characteristics of ATAC-seq publications. We then briefly introduced the principles of RNA-seq, ChIP-seq and ATAC-seq, focusing on the main features of the techniques. We built a phylogenetic tree from species that had been previously studied by using ATAC-seq. Studies of Mus musculus and Homo sapiens account for approximately 90% of the total ATAC-seq data, while other species are still in the process of accumulating data. We summarized the findings from human diseases and other species, illustrating the cutting-edge discoveries and the role of multi-omics data analysis in current research. Moreover, we collected and compared ATAC-seq analysis pipelines, which allowed biological researchers who lack programming skills to better analyze and explore ATAC-seq data. Through this review, it is clear that multi-omics analysis and single-cell sequencing technology will become the mainstream approach in future research.
MeSH term(s)	Animals ; Bibliometrics ; Chromatin Immunoprecipitation Sequencing ; Gene Expression ; High-Throughput Nucleotide Sequencing/methods ; Humans ; Mice ; Phylogeny ; RNA ; Sequence Analysis, DNA/methods
Chemical Substances	RNA (63231-63-0)
Language	English
Publishing date	2022-03-07
Publishing country	England
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	2068142-2
ISSN	1477-4054 ; 1467-5463
ISSN (online)	1477-4054
ISSN	1467-5463
DOI	10.1093/bib/bbac061
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Article ; Online: Fabrication of WPI-EGCG covalent conjugates/gellan gum double network emulsion gels by duo-induction of GDL and CaCl2 for colon-controlled Lactobacillus Plantarum delivery

Qin, Xinsheng / Bo, Qingli / Qin, Panzhu / Wang, Sufang / Liu, Kaiyong

Food Chemistry. 2023 Mar., v. 404 p.134513-

2023

Abstract: In this study, we demonstrated the role of whey protein isolate (WPI)/(−)-epigallocatechin-3-gallate (EGCG) covalent conjugates/gellangum double network emulsion gels by duo-induction of glucono-δ-lactone (GDL) and CaCl₂ on the digestion viability of ... ...

Abstract	In this study, we demonstrated the role of whey protein isolate (WPI)/(−)-epigallocatechin-3-gallate (EGCG) covalent conjugates/gellangum double network emulsion gels by duo-induction of glucono-δ-lactone (GDL) and CaCl₂ on the digestion viability of Lactobacillus Plantarum powders. We employed a novel one-step continuous cold gelation strategy to product double network emulsion gels. The gellan gum in continuous phase was first induced by CaCl₂ to form a gel in a short time. Besides, the GDL was improved covalent cross-linking between WPI-EGCG covalent conjugate Pickering particles or particle-coated oil droplets because of the slowly pH reduction. The first cross-linked gellan gum gels could be more closely integrated into the GDL-induced second gel networks with synergistic role, which promoted the formation of compact and homogeneous networks through hydrophobic interactions and disulfide bonds. Thus, the results provide a promising strategy for probiotic powders encapsulation of double network emulsion gels in oral applications of gel type foods.
Keywords	Lactobacillus plantarum ; cold ; crosslinking ; digestion ; disulfides ; emulsions ; encapsulation ; epigallocatechin gallate ; food chemistry ; gelation ; gellan gum ; gels ; hydrophobicity ; oils ; pH ; probiotics ; viability ; whey protein isolate ; Probiotic powders ; Double network emulsion gels ; Colon-controlled delivery ; WPI-EGCG covalent conjugates ; Duo-induction of GDL and CaCl2
Language	English
Dates of publication	2023-03
Publishing place	Elsevier Ltd
Document type	Article ; Online
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2022.134513
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Article ; Online: Development and validation of an improved QuEChERS method for the extraction of semi-volatile organic compounds (SVOCs) from complex soils.

Lou, Hongbo / Wang, Fujia / Zhao, Hangchen / Wang, Sufang / Xiao, Xinxin / Yang, Yanmei / Wang, Xiaowei

Analytical methods : advancing methods and applications

2023 Volume 15, Issue 36, Page(s) 4767–4776

Abstract: In order to achieve rapid, sensitive, and high-throughput determination of typical semi-volatile organic compounds (SVOCs) in soil samples, a method for the rapid determination of 63 SVOCs in soil was developed by optimizing and improving the QuEChERS ( ... ...

Abstract	In order to achieve rapid, sensitive, and high-throughput determination of typical semi-volatile organic compounds (SVOCs) in soil samples, a method for the rapid determination of 63 SVOCs in soil was developed by optimizing and improving the QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) extraction technique in conjunction with gas chromatography-mass spectrometry (GC-MS) analysis. A small amount of soil sample (5.0 g) was vortexed with 10 mL of a mixture of acetone and
Language	English
Publishing date	2023-09-21
Publishing country	England
Document type	Journal Article
ZDB-ID	2515210-5
ISSN	1759-9679 ; 1759-9660
ISSN (online)	1759-9679
ISSN	1759-9660
DOI	10.1039/d3ay01326j
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Article ; Online: Global, regional and national burden of retinopathy of prematurity among childhood and adolescent: a spatiotemporal analysis based on the Global Burden of Disease Study 2019.

Wang, Sufang / Liu, Jun / Zhang, Xiurong / Liu, Yonggang / Li, Jiaxin / Wang, Hao / Luo, Xiaoyang / Liu, Shunming / Liu, Lei / Zhang, Jie

BMJ paediatrics open

2024 Volume 8, Issue 1

Abstract: Background: This study aimed to provide a comprehensive assessment of burden estimates and the secular trend of vision loss due to retinopathy of prematurity (ROP) among people younger than 20 years, at the global, regional and national levels.: ... ...

Abstract	Background: This study aimed to provide a comprehensive assessment of burden estimates and the secular trend of vision loss due to retinopathy of prematurity (ROP) among people younger than 20 years, at the global, regional and national levels. Methods: Data were obtained from the Global Burden of Disease Study 2019 database. The average annual percentage change (AAPC) was calculated to quantify the temporal trends in the measures of vision loss. Results: In 2019, the global age-standardised rates (ASRs) of prevalence per 100 000 population was 86.4 for vision loss, specifically, 35 for moderate vision loss, 19.9 for severe vision loss, 31.6 for blindness due to ROP among people younger than 20 years. Moreover, the ASR of years lived with disability per 100 000 was 10.6 for vision loss, specifically, 1.1 for moderate vision loss, 3.6 for severe vision loss, 5.9 for blindness, respectively. From 1990 to 2019, the ASR of prevalence of blindness and vision loss due to ROP significantly increased, while its burden slightly decreased. Males showed higher ASR of prevalence than females in 2019, whereas females have larger increasing trend than males from 1990 to 2019. The global highest ASR of disease burden was observed in South Asia and Southern sub-Saharan Africa, as well as low sociodemographic index (SDI) regions in 2019. Conclusions: Globally, although the burden decreased, the prevalence of childhood and adulthood vision loss due to ROP continues to increase. Reasonable resource allocation and advanced intervention are recommended to prevent and control the vision loss due to ROP.
MeSH term(s)	Female ; Male ; Infant, Newborn ; Humans ; Adolescent ; Young Adult ; Adult ; Global Burden of Disease ; Retinopathy of Prematurity/epidemiology ; Blindness/epidemiology ; Blindness/etiology ; Spatio-Temporal Analysis ; Asia, Southern
Language	English
Publishing date	2024-01-06
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2399-9772
ISSN (online)	2399-9772
DOI	10.1136/bmjpo-2023-002267
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