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  1. Article: Multi-lineage Differentiation from Hematopoietic Stem Cells.

    Wang, Xiaoshuang / Liu, Siqi / Yu, Jia

    Advances in experimental medicine and biology

    2024  Volume 1442, Page(s) 159–175

    Abstract: The hematopoietic stem cells (HSCs) have the ability to differentiate and give rise to all mature blood cells. Commitment to differentiation progressively limits the self-renewal potential of the original HSCs by regulating the level of lineage-specific ... ...

    Abstract The hematopoietic stem cells (HSCs) have the ability to differentiate and give rise to all mature blood cells. Commitment to differentiation progressively limits the self-renewal potential of the original HSCs by regulating the level of lineage-specific gene expression. In this review, we will summarize the current understanding of the molecular mechanisms underlying HSC differentiation toward erythroid, myeloid, and lymphocyte lineages. Moreover, we will decipher how the single-cell technologies advance the lineage-biased HSC subpopulations and their differentiation potential.
    MeSH term(s) Hematopoietic Stem Cells ; Cell Differentiation/physiology ; Cell Lineage/genetics
    Language English
    Publishing date 2024-01-16
    Publishing country United States
    Document type Review ; Journal Article
    ZDB-ID 410187-X
    ISSN 0065-2598
    ISSN 0065-2598
    DOI 10.1007/978-981-99-7471-9_10
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Supervised Offseason Training Programs are able to mitigate the Effects of Detraining in Youth Men Soccer Players Physical Fitness: A Randomized Parallel Controlled Study.

    Liu, GuiYang / Wang, XiaoShuang / Xu, Qi

    Journal of sports science & medicine

    2024  Volume 23, Issue 1, Page(s) 219–227

    Abstract: This study aimed to analyze the effects of three off-season training programs on the aerobic capacity, countermovement jump (CMJ), and linear sprint performance of young male soccer players. The study employed a randomized multi-arm design, consisting of ...

    Abstract This study aimed to analyze the effects of three off-season training programs on the aerobic capacity, countermovement jump (CMJ), and linear sprint performance of young male soccer players. The study employed a randomized multi-arm design, consisting of three experimental groups: i) a high-intensity interval training (HIIT) group; (ii) a plyometric jump training (PJT) group; and (iii) a HIIT+PJT group; and an inactive control group. Fifty-eight under-19 male soccer players (aged 17.6 ±0.6 years) were randomly assigned to participate in a 3-week offseason training program exclusively performing HIIT, PJT, or a combination of both, while the fourth group remained inactive. Players underwent assessments twice, using the Yo-Yo Intermittent Recovery Test - Level 1 (YYIRT), CMJ, and 30-meter linear sprint. Significant interactions between time and groups were found in CMJ (p<0.001), YYIRT (p<0.001), and 30-m sprint (p<0.001). Group*time interaction revealed that the control group was significantly different from HIIT (p<0.001), PJT (p<0.001), and HIIT+PJT (p<0.001) considering the CMJ. Moreover, the control group was significantly different from HIIT (p=0.037) in YYIRT. Finally, the control group was significantly different from HIIT (p=0.024), PJT (p<0.001), and HIIT+PJT (p=0.021) considering the 30-m sprint. In conclusion, off-season training programs are effective in significantly reducing declines in CMJ and sprint performance compared to maintaining training cessation. However, in the YYIRT, only HIIT seems to be significantly superior to maintaining inactivity. To mitigate aerobic performance declines, incorporating HIIT sessions twice weekly during the offseason is advisable. To enhance or maintain jump performance, integrating at least one session of PJT weekly is beneficial.
    MeSH term(s) Humans ; Male ; Adolescent ; Soccer ; Athletic Performance ; Physical Fitness ; Plyometric Exercise ; High-Intensity Interval Training
    Language English
    Publishing date 2024-03-01
    Publishing country Turkey
    Document type Randomized Controlled Trial ; Journal Article
    ZDB-ID 2075564-8
    ISSN 1303-2968 ; 1303-2968
    ISSN (online) 1303-2968
    ISSN 1303-2968
    DOI 10.52082/jssm.2024.219
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Development, growth, and future of the Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College: 65 years tackling medical research paradigms.

    Wang, Xiaoshuang / Wang, Jing / Cheng, Tao

    Chinese medical journal

    2024  Volume 137, Issue 8, Page(s) 883–886

    MeSH term(s) Biomedical Research ; China ; Academies and Institutes ; Humans ; History, 20th Century ; History, 21st Century ; East Asian People
    Language English
    Publishing date 2024-03-19
    Publishing country China
    Document type Journal Article ; Historical Article
    ZDB-ID 127089-8
    ISSN 2542-5641 ; 0366-6999 ; 1002-0187
    ISSN (online) 2542-5641
    ISSN 0366-6999 ; 1002-0187
    DOI 10.1097/CM9.0000000000003050
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: [Prenatal diagnosis of two fetuses with 17q12 microdeletion syndrome].

    Wang, Xiaoshuang / Xu, Xiaoyuan

    Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics

    2022  Volume 39, Issue 10, Page(s) 1153–1157

    Abstract: Objective: To explore the genetic etiology of two fetuses with 17q12 microdeletion syndrome.: Methods: Chromosomal karyotype analysis, whole exome sequencing (WES) and chromosomal microarray analysis (CMA) were carried out for the fetuses. Relevant ... ...

    Abstract Objective: To explore the genetic etiology of two fetuses with 17q12 microdeletion syndrome.
    Methods: Chromosomal karyotype analysis, whole exome sequencing (WES) and chromosomal microarray analysis (CMA) were carried out for the fetuses. Relevant literature was searched in databases such as CNKI, Wanfang and PubMed to summarize the prenatal ultrasound finding, pregnancy outcome and clinical phenotype of the syndrome.
    Results: Both fetuses were found have renal parenchymal echo enhancement, accompanied by presence of renal cysts or hydramnios. Both were found to have a normal chromosomal karyotype, but had a 17q12 microdeletion by WES and CMA analysis. A total of 433 cases of 17q12 microdeletion syndromes have been reported in the literature, with renal cysts and diabetes as the most common phenotypes. Among 240 fetuses diagnosed with this syndrome, 72.9% showed unilateral or bilateral renal parenchymal echo enhancement, and 23.3% showed unilateral or bilateral renal cysts. Among these, 68 had reported the pregnancy outcome, for which 70.5% of pregnant women had opted termination of the pregnancy.
    Conclusion: WES and CMA can effectively detect 17q12 microdeletion. The clinical manifestations of this syndrome mainly include enhanced renal parenchymal echo, renal cyst, kidney disease and early-onset diabetes. Upon prenatal consultation, the prognosis of the fetus should be fully informed, and advice should be provided in combination with the preference of the couple, pregnancy history, family condition and other aspects.
    MeSH term(s) Chromosome Disorders/diagnosis ; Female ; Fetus ; Humans ; Karyotyping ; Kidney Diseases, Cystic ; Microarray Analysis ; Pregnancy ; Prenatal Diagnosis ; Syndrome
    Language Chinese
    Publishing date 2022-10-03
    Publishing country China
    Document type Journal Article
    ISSN 1003-9406
    ISSN 1003-9406
    DOI 10.3760/cma.j.cn511374-20210831-00712
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Higher final speed in 30-15 intermittent fitness tests correlates with soccer's locomotor demands, not heart rate responses in small-sided soccer games.

    Quan, YanXiu / Zhao, YongXing / Wang, XiaoShuang / Xu, Qi

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 10970

    Abstract: This study aimed to achieve two objectives: firstly, to analyze the relationships between aerobic fitness, as represented by the VIFT, and the heart rate and locomotor responses of youth male soccer players across various teams; and secondly, to compare ... ...

    Abstract This study aimed to achieve two objectives: firstly, to analyze the relationships between aerobic fitness, as represented by the VIFT, and the heart rate and locomotor responses of youth male soccer players across various teams; and secondly, to compare players with lower and higher VIFT in terms of performance outcomes extracted during small-sided games (SSGs). A total of twenty-six youth male soccer players, aged 16.5 ± 0.32 years, with 3.4 ± 1.1 years of experience, voluntarily participated in the study. These players belonged to two regional-level tier 2 teams (trained/developmental). In the initial week of observation, the 30-15 Intermittent Fitness Test was implemented to measure the final velocity (VIFT) achieved by the players. Subsequently, the 5v5 format of play was conducted twice a week over two consecutive weeks, during which heart rate responses and locomotor demands were measured. The Pearson product-moment correlation test revealed a significant correlation between VIFT and the total distance covered during the 5v5 format (r = 0.471 [95% CI: 0.093; 0.721], p = 0.015). Conversely, small and non-significant correlations were identified between VIFT and mean heart rate (r = 0.280 [95% CI: - 0.126; 0.598]; p = 0.166), VIFT and peak heart rate (r = 0.237 [95% CI: - 0.170; 0.569]; p = 0.243), as well as VIFT and high-speed running (r = 0.254 [95% CI: - 0.153; 0.580]; p = 0.211). Players with higher VIFT demonstrated a significantly greater total distance, with a large effect size (+ 6.64%; p = 0.015; d = 1.033), compared to those with lower VIFT. Our findings suggest that improved performance in VIFT may lead to covering more distance in 5v5 matches. However, the lack of significant associations between VIFT and heart rate levels during SSGs suggests that they are not strongly correlated, possibly because VIFT is more closely linked to locomotor profile. As a practical implication, coaches may consider organizing players during SSGs based on their VIFT if the goal is to standardize locomotor demands.
    MeSH term(s) Humans ; Soccer/physiology ; Heart Rate/physiology ; Male ; Adolescent ; Athletic Performance/physiology ; Running/physiology ; Physical Fitness/physiology ; Exercise Test/methods ; Locomotion/physiology
    Language English
    Publishing date 2024-05-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-61468-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Internal m 6 A and m 7 G RNA modifications in hematopoietic system and acute myeloid leukemia.

    Zhang, Xiaoxu / Ma, Yanni / Yu, Jia / Su, Rui / Wang, Xiaoshuang

    Chinese medical journal

    2024  Volume 137, Issue 9, Page(s) 1033–1043

    Abstract: Abstract: Epitranscriptomics focuses on the RNA-modification-mediated post-transcriptional regulation of gene expression. The past decade has witnessed tremendous progress in our understanding of the landscapes and biological functions of RNA ... ...

    Abstract Abstract: Epitranscriptomics focuses on the RNA-modification-mediated post-transcriptional regulation of gene expression. The past decade has witnessed tremendous progress in our understanding of the landscapes and biological functions of RNA modifications, as prompted by the emergence of potent analytical approaches. The hematopoietic system provides a lifelong supply of blood cells, and gene expression is tightly controlled during the differentiation of hematopoietic stem cells (HSCs). The dysregulation of gene expression during hematopoiesis may lead to severe disorders, including acute myeloid leukemia (AML). Emerging evidence supports the involvement of the mRNA modification system in normal hematopoiesis and AML pathogenesis, which has led to the development of small-molecule inhibitors that target N6-methyladenosine (m 6 A) modification machinery as treatments. Here, we summarize the latest findings and our most up-to-date information on the roles of m 6 A and N7-methylguanine in both physiological and pathological conditions in the hematopoietic system. Furthermore, we will discuss the therapeutic potential and limitations of cancer treatments targeting m 6 A.
    MeSH term(s) Humans ; Leukemia, Myeloid, Acute/genetics ; Leukemia, Myeloid, Acute/metabolism ; Adenosine/analogs & derivatives ; Adenosine/metabolism ; Hematopoietic System ; Hematopoietic Stem Cells/metabolism ; Hematopoiesis/genetics ; RNA Processing, Post-Transcriptional/genetics
    Chemical Substances Adenosine (K72T3FS567) ; N-methyladenosine (CLE6G00625)
    Language English
    Publishing date 2024-03-28
    Publishing country China
    Document type Journal Article ; Review
    ZDB-ID 127089-8
    ISSN 2542-5641 ; 0366-6999 ; 1002-0187
    ISSN (online) 2542-5641
    ISSN 0366-6999 ; 1002-0187
    DOI 10.1097/CM9.0000000000003073
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Baicalin suppress the development of polycystic ovary syndrome via regulating the miR-874-3p/FOXO3 and miR-144/FOXO1 axis.

    Xu, Xiaoyuan / Xu, Xiaohua / Wang, Xiaoshuang / Shen, Ling

    Pharmaceutical biology

    2023  Volume 61, Issue 1, Page(s) 878–885

    Abstract: Context: Polycystic ovary syndrome (PCOS) is a common and complex disease caused by endocrine and metabolic dysfunction in women of reproductive age. Baicalin is reported to ameliorate PCOS.: Objective: This study determines whether baicalin could ... ...

    Abstract Context: Polycystic ovary syndrome (PCOS) is a common and complex disease caused by endocrine and metabolic dysfunction in women of reproductive age. Baicalin is reported to ameliorate PCOS.
    Objective: This study determines whether baicalin could affect the progression of PCOS.
    Materials and methods: To establish an animal model of PCOS, female Sprague-Dawley (SD) rats were subcutaneously injected with dehydroepiandrosterone (DHEA, 60 mg/kg) for 20 days. Next, normal and PCOS mice were divided into 3 groups: control, PCOS, PCOS + Baicalin (20 mg/kg) groups. In addition, the levels of microRNA-874-3p (miR-874-3p) and microRNA-144 (miR-144) in ovarian tissues were assessed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR).
    Results: Compared to the PCOS group, baicalin treatment significantly declined free testosterone (33.71 pg/mL vs. 56.05 pg/mL) and luteinizing hormone (LH; 3971.73 pg/mL vs. 5201.50 pg/mL) levels in rats with PCOS. Additionally, compared to the control group, 100 μM baicalin lessened miR-874-3p and miR-144 levels in human ovarian granulosa cells (KGN cells) by 36.87% and 32.57%, respectively. Furthermore, forkhead box O (FOXO) proteins FOXO1 and FOXO3 are the direct targets of miR-144 and miR-874-3p, respectively. Meanwhile, baicalin induced G0-G1 phase arrest (69.56 ± 3.7% at baicalin with 100 μM vs. 51.24 ± 3.2%, control) in KGN cells correlating with decreased p27 Kip1 (FOXO proteins downstream effector gene) expression by 55.5%; however, miR-874-3p or miR-144 overexpression could abolish this effect.
    Conclusions: Baicalin could alleviate the symptoms of PCOS
    MeSH term(s) Humans ; Female ; Rats ; Mice ; Animals ; Polycystic Ovary Syndrome/chemically induced ; Polycystic Ovary Syndrome/drug therapy ; Polycystic Ovary Syndrome/genetics ; Rats, Sprague-Dawley ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Apoptosis ; Cell Proliferation/genetics ; Forkhead Box Protein O1 ; Forkhead Box Protein O3/genetics
    Chemical Substances MIRN874 microRNA, mouse ; baicalin (347Q89U4M5) ; MicroRNAs ; FOXO1 protein, human ; Forkhead Box Protein O1 ; FOXO3 protein, human ; Forkhead Box Protein O3 ; MIRN144 microRNA, human ; MIRN874 microRNA, human ; Foxo1 protein, mouse ; MIRN144 microRNA, mouse ; Foxo1 protein, rat ; MIRN144 microRNA, rat
    Language English
    Publishing date 2023-06-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 1440131-9
    ISSN 1744-5116 ; 1388-0209
    ISSN (online) 1744-5116
    ISSN 1388-0209
    DOI 10.1080/13880209.2023.2208636
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Efficacy of roxadustat in maintenance hemodialysis patients with erythropoietin-hyporesponsive anemia.

    Wang, Xiaoshuang / Cai, Hezhou / Xu, Haifan / Chen, Liqun

    Clinical nephrology

    2023  Volume 101, Issue 1, Page(s) 25–33

    Abstract: Objective: To investigate the efficacy of roxadustat in hemodialysis patients with erythropoietin (EPO) hypo-responsive anemia.: Materials and methods: This retrospective study included 55 hemodialysis patients with erythropoietin hypo-responsive ... ...

    Abstract Objective: To investigate the efficacy of roxadustat in hemodialysis patients with erythropoietin (EPO) hypo-responsive anemia.
    Materials and methods: This retrospective study included 55 hemodialysis patients with erythropoietin hypo-responsive anemia at the First Affiliated Hospital of Chongqing Medical University from January to December 2020. We observed their hemoglobin (Hb) changes, inflammatory factors, and adverse reactions before and after 12 weeks of roxadustat treatment.
    Results: Among the 55 patients, the average age was 60.75 ± 13.96 years old, and the median dialysis age was 48 months. All patients were taken off EPO and switched to roxadustat during the follow-up period. Compared with baseline, patients' Hb was significantly increased (90.64 ± 20.01 g/L, 98.52 ± 15.89 g/L, 104.34 ± 19.15 g/L, and 107.02 ± 20.54 g/L, respectively) (p < 0.05). At 12 weeks of roxadustat treatment, 34 patients (61.82%) met the target Hb levels (100 - 130 g/L). The multivariate logistic analysis showed that Hb response positively correlated with the Hb level before roxadustat treatment (p = 0.046), while responding well to roxadustat negatively correlated with blood platelet-to-lymphocyte ratio (PLR) and duration of dialysis (p = 0.029 and p = 0.046) in patients with EPO hypo-responsive anemia.
    Conclusion: Roxadustat could effectively improve anemia; the PLR and dialysis age were independent predictors of roxadustat efficacy in dialysis patients with EPO hypo-responsive anemia.
    MeSH term(s) Humans ; Middle Aged ; Anemia/drug therapy ; Anemia/etiology ; Epoetin Alfa ; Erythropoietin/therapeutic use ; Glycine/adverse effects ; Hemoglobins/analysis ; Isoquinolines/adverse effects ; Renal Dialysis/adverse effects ; Retrospective Studies
    Chemical Substances Epoetin Alfa (64FS3BFH5W) ; Erythropoietin (11096-26-7) ; Glycine (TE7660XO1C) ; Hemoglobins ; Isoquinolines
    Language English
    Publishing date 2023-11-16
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 185101-9
    ISSN 0301-0430
    ISSN 0301-0430
    DOI 10.5414/CN111109
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: N-doped carbon dots for the determination of Al

    Yang, Dou / Shao, Tong / Wang, Xiaoshuang / Hong, Min / Li, Rui / Li, Chenzhong / Yue, Qiaoli

    Mikrochimica acta

    2024  Volume 191, Issue 1, Page(s) 78

    Abstract: New portable hydrogel sensors for ... ...

    Abstract New portable hydrogel sensors for Al
    Language English
    Publishing date 2024-01-05
    Publishing country Austria
    Document type Journal Article
    ZDB-ID 89-9
    ISSN 1436-5073 ; 0026-3672
    ISSN (online) 1436-5073
    ISSN 0026-3672
    DOI 10.1007/s00604-023-06143-w
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  10. Article ; Online: A novel pectin polysaccharide from vinegar-baked Radix Bupleuri absorbed by microfold cells in the form of nanoparticles.

    Zhao, Ya / Li, Ping / Wang, Xiaoshuang / Wu, Yayun / Liu, Lijuan / Zhao, Ruizhi

    International journal of biological macromolecules

    2024  Volume 266, Issue Pt 1, Page(s) 131096

    Abstract: Polysaccharides of vinegar-baked Radix Bupleuri (VBCP) have been reported to exhibit liver-targeting and immunomodulatory activities through oral administration, but the absorption behavior and mechanism of VBCPs have not been extensively studied. In ... ...

    Abstract Polysaccharides of vinegar-baked Radix Bupleuri (VBCP) have been reported to exhibit liver-targeting and immunomodulatory activities through oral administration, but the absorption behavior and mechanism of VBCPs have not been extensively studied. In this study, a novel HG type pectin polysaccharide, VBCP1-4, with a high molecular weight of 2.94 × 10
    MeSH term(s) Pectins/chemistry ; Bupleurum/chemistry ; Acetic Acid/chemistry ; Nanoparticles/chemistry ; Humans ; Animals ; Caco-2 Cells ; Particle Size ; Molecular Weight ; M Cells
    Chemical Substances Pectins (89NA02M4RX) ; Acetic Acid (Q40Q9N063P)
    Language English
    Publishing date 2024-03-24
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2024.131096
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