Article ; Online: hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue.
2020 Volume 10, Issue 1, Page(s) 9950
Abstract: Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). ... ...
| Abstract | Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5 mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) fixation as compared to the more commonly used 4% paraformaldehyde (PFA) on clearing performance. |
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| MeSH term(s) | Affinity Labels/chemistry ; Animals ; Brain/anatomy & histology ; Brain/cytology ; Carbocyanines/chemistry ; Fluorescent Dyes/chemistry ; Formaldehyde/chemistry ; Humans ; Lipids/chemistry ; Lysine/chemistry ; Mice ; Periodic Acid/chemistry ; Swine ; Tissue Fixation/methods |
| Chemical Substances | Affinity Labels ; Carbocyanines ; Fluorescent Dyes ; Lipids ; periodate-lysine-paraformaldehyde ; Periodic Acid (10450-60-9) ; Formaldehyde (1HG84L3525) ; 3,3'-dioctadecylindocarbocyanine (40957-95-7) ; Lysine (K3Z4F929H6) |
| Language | English |
| Publishing date | 2020-06-19 |
| Publishing country | England |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2615211-3 |
| ISSN | 2045-2322 ; 2045-2322 |
| ISSN (online) | 2045-2322 |
| ISSN | 2045-2322 |
| DOI | 10.1038/s41598-020-66999-3 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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