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  1. Article ; Online: Screening and characterization of agarolytic bacteria from different sources.

    Labade, Dinesh / Tabassum, Heena / Wani, Minal

    Letters in applied microbiology

    2023  Volume 76, Issue 7

    Abstract: According to the results of our investigation, distinct bacterial isolates capable of breaking down agar were found in various nonmarine environments. The deficiency of reducing sugar in the control media demonstrates that the agar in the experiment is ... ...

    Abstract According to the results of our investigation, distinct bacterial isolates capable of breaking down agar were found in various nonmarine environments. The deficiency of reducing sugar in the control media demonstrates that the agar in the experiment is broken down by the bacteria to produce various oligosaccharides because the viscosity of the medium containing the agar was found to have been extremely high before inoculation, reducing with incubation duration and attaining a maximum after 48 hours. These isolates were subsequently used in tests along with additional investigation since they could create reducing sugar. Interestingly, the deterioration of agar appears to be mainly caused by Gram-negative bacteria. In order to study the agarase properties, the relative quantity of the enzyme secreted by the bacteria that hydrolyze the agar was used. The detection of extracellular agarase surrounding the colonies and the absence of stained halos on iodine-treated agar plates show that the agarase diffusing from the bacteria impacted the characteristics of the gel. Inconclusion, these agarsase-producing bacteria can be exploited for industrial applications. Waste agar from the plant tissue culture business can be utilized for a range of applications and this degraded agar can be explored for reliable and ecologically safe alternatives.
    MeSH term(s) Agar/metabolism ; Bacteria/metabolism ; Gram-Negative Bacteria ; Glycoside Hydrolases/metabolism ; Oligosaccharides ; Sugars
    Chemical Substances Agar (9002-18-0) ; Glycoside Hydrolases (EC 3.2.1.-) ; Oligosaccharides ; Sugars
    Language English
    Publishing date 2023-07-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 632584-1
    ISSN 1472-765X ; 0266-8254
    ISSN (online) 1472-765X
    ISSN 0266-8254
    DOI 10.1093/lambio/ovad079
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  2. Article ; Online: Nigella sativa

    Gawas, Chaitali G / Mathur, Sakshi / Wani, Minal / Tabassum, Heena

    Ibrain

    2023  Volume 9, Issue 1, Page(s) 111–123

    Abstract: ... Nigella ... ...

    Abstract Nigella sativa
    Language English
    Publishing date 2023-02-07
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 2769-2795
    ISSN (online) 2769-2795
    DOI 10.1002/ibra.12091
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  3. Article ; Online: Statistical optimization of process variables for agarase production using

    Labade, Dinesh / Sevamani, Selvaraju / Tabassum, Heena / Madhyastha, Harishkumar / Wani, Minal

    Preparative biochemistry & biotechnology

    2023  Volume 54, Issue 3, Page(s) 393–406

    Abstract: Agar oligosaccharides are thought to be valuable biomolecules with high bioactivity potential, along with a wide range of applications and advantages. The current study aimed to optimize the culture parameters required to produce agarase enzyme and agar ... ...

    Abstract Agar oligosaccharides are thought to be valuable biomolecules with high bioactivity potential, along with a wide range of applications and advantages. The current study aimed to optimize the culture parameters required to produce agarase enzyme and agar oligosaccharides from industrial waste agar.
    MeSH term(s) Agar/chemistry ; Microbacterium ; Temperature ; Oligosaccharides ; Glycoside Hydrolases
    Chemical Substances Agar (9002-18-0) ; agarase (EC 3.2.1.81) ; Oligosaccharides ; Glycoside Hydrolases (EC 3.2.1.-)
    Language English
    Publishing date 2023-09-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 1322522-4
    ISSN 1532-2297 ; 1082-6068
    ISSN (online) 1532-2297
    ISSN 1082-6068
    DOI 10.1080/10826068.2023.2245866
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  4. Article ; Online: Neurodegenerative disorders: Assessing the impact of natural vs drug-induced treatment options.

    Mathur, Sakshi / Gawas, Chaitali / Ahmad, Iffat Zareen / Wani, Minal / Tabassum, Heena

    Aging medicine (Milton (N.S.W))

    2023  Volume 6, Issue 1, Page(s) 82–97

    Abstract: Neurodegenerative illnesses refer to the gradual, cumulative loss of neural activity. Neurological conditions are considered to be the second leading cause of mortality in the modern world and the two most prevalent ones are Parkinson's disease and ... ...

    Abstract Neurodegenerative illnesses refer to the gradual, cumulative loss of neural activity. Neurological conditions are considered to be the second leading cause of mortality in the modern world and the two most prevalent ones are Parkinson's disease and Alzheimer's disease. The negative side effects of pharmaceutical use are a major global concern, despite the availability of many different treatments for therapy. We concentrated on different types of neurological problems and their influence on targets, in vitro, in vivo, and in silico methods toward neurological disorders, as well as the molecular approaches influencing the same, in the first half of the review. The bulk of the second half of the review focuses on the many categories of treatment possibilities, including natural and artificial. Nevertheless, herbal treatment solutions are piquing scholarly attention due to their anti-oxidative properties and accessibility. However, more quality investigations and innovations are undoubtedly needed to back up these conclusions.
    Language English
    Publishing date 2023-02-22
    Publishing country Australia
    Document type Journal Article ; Review
    ISSN 2475-0360
    ISSN (online) 2475-0360
    DOI 10.1002/agm2.12243
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  5. Article ; Online: We are all aging, and here's why.

    Shinde, Atharva / Deore, Gargi / Navsariwala, Kedar P / Tabassum, Heena / Wani, Minal

    Aging medicine (Milton (N.S.W))

    2022  Volume 5, Issue 3, Page(s) 211–231

    Abstract: Here, through this review, we aim to serve this purpose by first discussing the statistics and aging demographics, including the life expectancy of the world and India, along with the gender life expectancy gap observed throughout the world, followed by ... ...

    Abstract Here, through this review, we aim to serve this purpose by first discussing the statistics and aging demographics, including the life expectancy of the world and India, along with the gender life expectancy gap observed throughout the world, followed by explaining the hallmarks and integral causes of aging, along with the role played by senescent cells in controlling inflammation and the effect of senescence associated secretory phenotype on longevity. A few of the molecular pathways which are crucial in modulating the process of aging, such as the nutrient-sensing mTOR pathway, insulin signaling, Nrf2, FOXO, PI3-Akt, Sirtuins, and AMPK, and their effects are also covered in paramount detail. A diverse number of ingenious research methodologies are used in the modern era of longevity exploration. We have attempted to cover these methods under the umbrella of three broad categories: in vitro, in vivo, and in silico techniques. The drugs developed to attenuate the aging process, such as rapamycin, metformin, resveratrol, etc. and their interactions with the above-mentioned molecular pathways along with their toxicity have also been reviewed in detail.
    Language English
    Publishing date 2022-10-03
    Publishing country Australia
    Document type Journal Article ; Review
    ISSN 2475-0360
    ISSN (online) 2475-0360
    DOI 10.1002/agm2.12223
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  6. Article ; Online: Screening of potential phytomolecules against MurG as drug target in nosocomial pathogen

    Lokhande, Kiran Bharat / Pawar, Sarika Vishnu / Madkaiker, Smriti / Shrivastava, Ashish / Venkateswara, Swamy K / Nawani, Neelu / Wani, Minal / Ghosh, Payel / Singh, Ashutosh

    Journal of biomolecular structure & dynamics

    2023  Volume 42, Issue 1, Page(s) 495–508

    Abstract: The nosocomial infection outbreak caused ... ...

    Abstract The nosocomial infection outbreak caused by
    MeSH term(s) Humans ; Pseudomonas aeruginosa ; Cross Infection ; Theobromine ; Molecular Docking Simulation ; Molecular Dynamics Simulation
    Chemical Substances petasiphenol (145904-50-3) ; demethoxycurcumin (W2F8059T80) ; Theobromine (OBD445WZ5P)
    Language English
    Publishing date 2023-03-28
    Publishing country England
    Document type Journal Article
    ZDB-ID 49157-3
    ISSN 1538-0254 ; 0739-1102
    ISSN (online) 1538-0254
    ISSN 0739-1102
    DOI 10.1080/07391102.2023.2194005
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  7. Article ; Online: Computational docking investigation of phytocompounds from bergamot essential oil against Serratia marcescens protease and FabI: Alternative pharmacological strategy.

    Lokhande, Kiran Bharat / Tiwari, Ayushi / Gaikwad, Swapnil / Kore, Supriya / Nawani, Neelu / Wani, Minal / Swamy, K Venkateswara / Pawar, Sarika Vishnu

    Computational biology and chemistry

    2023  Volume 104, Page(s) 107829

    Abstract: The rapid development of multi-drug resistant (MDR) pathogens adds urgency to search for novel and safe drugs having promising action on new and re-emerging infectious pathogens. Serratia marcescens is an MDR pathogen that causes several-healthcare ... ...

    Abstract The rapid development of multi-drug resistant (MDR) pathogens adds urgency to search for novel and safe drugs having promising action on new and re-emerging infectious pathogens. Serratia marcescens is an MDR pathogen that causes several-healthcare associated infections. Curbing bacterial virulence, rather than inhibiting its growth, is a promising strategy to diminish the pathogenesis of infectious bacteria, reduce the development of antimicrobial resistance, and boost the host immune power to eradicate infections. Bergamot essential oil (BEO) is a remarkable source of promising therapeutics against pathogens. Therefore, the present investigation aimed to analyze the major phytocompounds from BEO against S. marcescens virulent proteins using in silico studies. The analysis of BEO phytocompounds was achieved by Gas chromatography-mass spectrometry (GC-MS) method. The molecular docking was carried out using the SP and XP docking protocol of the Glide program. The drug-likeness and pharmacokinetics properties (ADMET properties) were analyzed with SwissADME and pkCSM server. The results revealed that the major compounds present in BEO are Linalool (8.17%), D-Limonene (21.26%), and Linalyl acetate (26.91%). Molecular docking analysis revealed that these compounds docked strongly within the binding cavities of Serratia protease and FabI model which in turn curb the pathogenesis of this bacteria. Linalool interacted with the Serratia protease and FabI with a binding energy of - 3.130 kcal/mol and - 3.939 kcal/mol, respectively. Based on the pharmacokinetics findings all lead BEO phytocompounds appear to be promising drug candidates. Overall, these results represent a significant step in the development of plant-based compounds as a promising inhibitor of the virulent proteins of the MDR S. marcescens.
    MeSH term(s) Oils, Volatile/pharmacology ; Oils, Volatile/chemistry ; Serratia marcescens ; Peptide Hydrolases ; Molecular Docking Simulation
    Chemical Substances Oils, Volatile ; linalool (D81QY6I88E) ; Peptide Hydrolases (EC 3.4.-)
    Language English
    Publishing date 2023-02-15
    Publishing country England
    Document type Journal Article
    ISSN 1476-928X
    ISSN (online) 1476-928X
    DOI 10.1016/j.compbiolchem.2023.107829
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  8. Article ; Online: Hepatitis B virus DNA polymerase gene polymorphism based prediction of genotypes in chronic HBV patients from Western India.

    Chavan, Yashwant G / Pawar, Sharad R / Wani, Minal / Raut, Amol D / Misra, Rabindra N

    African health sciences

    2017  Volume 17, Issue 3, Page(s) 762–772

    Abstract: Background: Hepatitis B Virus (HBV) infection is one of the major causes of liver cirrhosis, hepatocellular carcinoma and deaths due to the acute or chronic consequences worldwide. HBV is distributed into various genotypes based on nucleic acid sequence ...

    Abstract Background: Hepatitis B Virus (HBV) infection is one of the major causes of liver cirrhosis, hepatocellular carcinoma and deaths due to the acute or chronic consequences worldwide. HBV is distributed into various genotypes based on nucleic acid sequence variation.
    Objectives: To develop a method of HBV genotyping and drug resistance interpretation using partial sequencing of polymerase gene.
    Methods: This study was performed on 98 HBV infected patients' serum samples from Western India. A nested PCR protocol was designed for amplification of pol gene from HBV genome and Sanger's sequencing of the gene fragment. Sequences were aligned with HBV reference sequences for phylogenetic analysis and for characterization of genetic diversity. Drug resistance mutations were screened using HBVSeq program from Stanford University.
    Results: Distribution of HBV genotypes showed predominance of genotype D, circulating in 76 (77.55%) patients (p < 0.05). Genotypes A and C were less prevalent and were identified in 4 (4.08%) and 18 (18.37%) patients, respectively. Anti-retroviral drug resistance mutations were not detected in any patient.
    Conclusion: A method for determination of HBV genotypes using pol gene sequencing which simultaneously detects major drug resistance mutations has been established. HBV genetic diversity may play an important role in treatment decision.
    Language English
    Publishing date 2017-09
    Publishing country Uganda
    Document type Journal Article
    ZDB-ID 2240308-5
    ISSN 1729-0503 ; 1680-6905
    ISSN (online) 1729-0503
    ISSN 1680-6905
    DOI 10.4314/ahs.v17i3.19
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    Zs.A 3851: Show issues Location:
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  9. Article ; Online: Development, validation and clinical evaluation of a low cost in-house HIV-1 drug resistance genotyping assay for Indian patients.

    Acharya, Arpan / Vaniawala, Salil / Shah, Parth / Misra, Rabindra Nath / Wani, Minal / Mukhopadhyaya, Pratap N

    PloS one

    2014  Volume 9, Issue 8, Page(s) e105790

    Abstract: Human Immunodeficiency Virus-1 (HIV-1) drug resistance genotyping assay is a part of clinical management of HIV-1 positive individuals under treatment with highly active antiretroviral therapy (HAART). Routine monitoring of drug resistance mutations in ... ...

    Abstract Human Immunodeficiency Virus-1 (HIV-1) drug resistance genotyping assay is a part of clinical management of HIV-1 positive individuals under treatment with highly active antiretroviral therapy (HAART). Routine monitoring of drug resistance mutations in resource limited settings like India is not possible due to high cost of commercial drug resistance assays. In this study we developed an in-house, cost effective HIV-1 drug resistance genotyping assay for Indian patients and validated it against the US-FDA-approved ViroSeq HIV-1 drug resistance testing system. A reference panel of 20 clinical samples was used to develop and validate the assay against ViroSeq HIV-1 drug resistance testing system which was subsequently used to genotype a clinical panel of 225 samples. The Stanford HIV database was used to identify drug resistant mutations. The analytical sensitivity of the assay was 1000 HIV-1 RNA copies/ml of plasma sample while precision and reproducibility was 99.68 ± 0.16% and 99.76 ± 0.18% respectively. One hundred and one drug resistant mutations were detected by the in-house assay compared to 104 by ViroSeq system in the reference panel. The assay had 91.55% success rate in genotyping the clinical panel samples and was able to detect drug resistant mutations related to nucleoside reverse transcriptase inhibitor (NRTI), non-nucleoside reverse-transcriptase inhibitor (NNRTI) as well as protease inhibitor (PI) classes of antiretroviral drugs. It was found to be around 71.9% more cost effective compared to ViroSeq genotyping system. This evaluation of the assay on the clinical panel demonstrates its potential for monitoring clinical HIV-1 drug resistance mutations and population-based surveillance in resource limited settings like India.
    MeSH term(s) Adult ; Anti-HIV Agents/pharmacology ; Cost-Benefit Analysis ; DNA Mutational Analysis ; Drug Resistance, Viral/genetics ; Female ; Genotyping Techniques/economics ; HIV Infections/drug therapy ; HIV Infections/virology ; HIV-1/drug effects ; HIV-1/genetics ; Humans ; India ; Male ; Phylogeny ; RNA, Viral/genetics ; RNA, Viral/isolation & purification ; Reproducibility of Results ; Sensitivity and Specificity
    Chemical Substances Anti-HIV Agents ; RNA, Viral
    Language English
    Publishing date 2014-08-26
    Publishing country United States
    Document type Journal Article ; Validation Study
    ISSN 1932-6203
    ISSN (online) 1932-6203
    DOI 10.1371/journal.pone.0105790
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  10. Article: In vitro callus induction and estimation of plumbagin content from Plumbago auriculata Lam.

    Deshpande, Jyoti / Labade, Dinesh / Shankar, Kalaiselvi / Kata, Navin / Chaudhari, Manoj / Wani, Minal / Khetmalas, Madhukar

    Indian journal of experimental biology

    2014  Volume 52, Issue 11, Page(s) 1122–1127

    Abstract: The medicinal plant Plumbago contains a very potent secondary metabolite, plumbagin having many therapeutic properties. Callus culture was induced using explants, leaf, stem and shoot apex, from P. auriculata. Murashige and Skoog media fortified with ... ...

    Abstract The medicinal plant Plumbago contains a very potent secondary metabolite, plumbagin having many therapeutic properties. Callus culture was induced using explants, leaf, stem and shoot apex, from P. auriculata. Murashige and Skoog media fortified with various growth hormones like NAA, IAA, IBA and 2, 4-D individually and in various combinations were checked for callus induction. Among the growth hormones used, 1 mg/L 2, 4-D showed best callusing. The hormonal combinations of 1 mg/L IAA and 1.5 mg/L NAA in the media exhibited best callus induction using stem internode as an explant. Plumbagin content from root, stem, leaf and callus was analyzed by using thin layer chromatographic technique. The callus derived from stem showed comparable plumbagin content to the in vivo plant parts. Quantitative spectrophotometric analysis of plumbagin from plant samples and callus indicated that plumbagin content was maximum in roots which was followed by callus, stem and leaf samples respectively. Generation of in vitro sources for p!umbagin, for therapeutic applications will serve as a continuous supply and will contribute to preserve the natural plant recourses.
    MeSH term(s) Chromatography, Thin Layer ; Colorimetry ; Cytokinins/pharmacology ; Indoleacetic Acids/pharmacology ; Naphthoquinones/analysis ; Naphthoquinones/metabolism ; Organ Specificity ; Organoids/drug effects ; Plant Cells/drug effects ; Plant Leaves/metabolism ; Plant Roots/metabolism ; Plant Shoots/metabolism ; Plant Stems/metabolism ; Plants, Medicinal/growth & development ; Plants, Medicinal/metabolism ; Plumbaginaceae/growth & development ; Plumbaginaceae/metabolism ; Tissue Culture Techniques
    Chemical Substances Cytokinins ; Indoleacetic Acids ; Naphthoquinones ; plumbagin (YAS4TBQ4OQ)
    Language English
    Publishing date 2014-11
    Publishing country India
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 416061-7
    ISSN 0975-1009 ; 0019-5189
    ISSN (online) 0975-1009
    ISSN 0019-5189
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