LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 7 of total 7

Search options

  1. Article ; Online: Seed Development and Protein Accumulation Patterns in Faba Bean (

    Warsame, Ahmed O / Michael, Nicholas / O'Sullivan, Donal M / Tosi, Paola

    Journal of agricultural and food chemistry

    2022  Volume 70, Issue 30, Page(s) 9295–9304

    Abstract: A major objective in faba bean breeding is to improve its protein quality by selecting cultivars with enhanced desirable physicochemical properties. However, the protein composition of the mature seed is determined by a series of biological processes ... ...

    Abstract A major objective in faba bean breeding is to improve its protein quality by selecting cultivars with enhanced desirable physicochemical properties. However, the protein composition of the mature seed is determined by a series of biological processes occurring during seed growth. Thus, any attempt to explain the final seed composition must consider the dynamics of the seed proteome during seed development. Here, we investigated the proteomic profile of developing faba bean seeds across 12 growth stages from 20 days after pollination (DAP) to full maturity. We analyzed trypsin-digested total protein extracts from the seeds at different growth stages by liquid chromatography-tandem mass spectrometry (LC-MS/MS), identifying 1217 proteins. The functional clusters of these proteins showed that, in early growth stages, proteins related to cell growth, division, and metabolism were most abundant compared to seed storage proteins that began to accumulate from 45 DAP. Moreover, label-free quantification of the relative abundance of seed proteins, including important globulin proteins, revealed several distinct temporal accumulation trends among the protein classes. These results suggest that these proteins are regulated differently and require further understanding of the impact of the different environmental stresses occurring at different grain filling stages on the expression and accumulation of these seed storage proteins.
    MeSH term(s) Chromatography, Liquid ; Plant Breeding ; Proteomics ; Seed Storage Proteins/metabolism ; Seeds/chemistry ; Tandem Mass Spectrometry ; Vicia faba/chemistry
    Chemical Substances Seed Storage Proteins
    Language English
    Publishing date 2022-07-21
    Publishing country United States
    Document type Journal Article
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.2c02061
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1172: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article: Seed Development and Protein Accumulation Patterns in Faba Bean (Vicia faba, L.)

    Warsame, Ahmed O. / Michael, Nicholas / O’Sullivan, Donal M. / Tosi, Paola

    Journal of agricultural and food chemistry. 2022 July 21, v. 70, no. 30

    2022  

    Abstract: A major objective in faba bean breeding is to improve its protein quality by selecting cultivars with enhanced desirable physicochemical properties. However, the protein composition of the mature seed is determined by a series of biological processes ... ...

    Abstract A major objective in faba bean breeding is to improve its protein quality by selecting cultivars with enhanced desirable physicochemical properties. However, the protein composition of the mature seed is determined by a series of biological processes occurring during seed growth. Thus, any attempt to explain the final seed composition must consider the dynamics of the seed proteome during seed development. Here, we investigated the proteomic profile of developing faba bean seeds across 12 growth stages from 20 days after pollination (DAP) to full maturity. We analyzed trypsin-digested total protein extracts from the seeds at different growth stages by liquid chromatography-tandem mass spectrometry (LC-MS/MS), identifying 1217 proteins. The functional clusters of these proteins showed that, in early growth stages, proteins related to cell growth, division, and metabolism were most abundant compared to seed storage proteins that began to accumulate from 45 DAP. Moreover, label-free quantification of the relative abundance of seed proteins, including important globulin proteins, revealed several distinct temporal accumulation trends among the protein classes. These results suggest that these proteins are regulated differently and require further understanding of the impact of the different environmental stresses occurring at different grain filling stages on the expression and accumulation of these seed storage proteins.
    Keywords Vicia faba ; cell growth ; cultivars ; faba beans ; food chemistry ; globulins ; liquid chromatography ; metabolism ; pollination ; protein content ; protein value ; proteome ; proteomics ; seed growth ; seed storage ; tandem mass spectrometry
    Language English
    Dates of publication 2022-0721
    Size p. 9295-9304.
    Publishing place American Chemical Society
    Document type Article
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.2c02061
    Database NAL-Catalogue (AGRICOLA)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 1185: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: Identification and Quantification of Major Faba Bean Seed Proteins.

    Warsame, Ahmed O / Michael, Nicholas / O'Sullivan, Donal M / Tosi, Paola

    Journal of agricultural and food chemistry

    2020  Volume 68, Issue 32, Page(s) 8535–8544

    Abstract: Faba bean ( ...

    Abstract Faba bean (
    MeSH term(s) Chromatography, High Pressure Liquid ; Electrophoresis, Polyacrylamide Gel ; Mass Spectrometry ; Plant Proteins/chemistry ; Seeds/chemistry ; Vicia faba/chemistry
    Chemical Substances Plant Proteins
    Language English
    Publishing date 2020-07-28
    Publishing country United States
    Document type Journal Article
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.0c02927
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1172: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Seed Storage Proteins of Faba Bean ( Vicia faba L): Current Status and Prospects for Genetic Improvement.

    Warsame, Ahmed O / O'Sullivan, Donal M / Tosi, Paola

    Journal of agricultural and food chemistry

    2018  Volume 66, Issue 48, Page(s) 12617–12626

    Abstract: Faba bean ( Vicia faba L.) is one of the foremost candidate crops for simultaneously increasing both sustainability and global supply of plant protein. On a dry matter basis, its seeds contain about 29% protein of which more than 80% consists of globulin ...

    Abstract Faba bean ( Vicia faba L.) is one of the foremost candidate crops for simultaneously increasing both sustainability and global supply of plant protein. On a dry matter basis, its seeds contain about 29% protein of which more than 80% consists of globulin storage proteins (vicilin and legumin). However, to achieve optimum utilization of this crop for human and animal nutrition, both protein content and quality have to be improved. Though initial investigations on the heritability of these traits indicated the possibility for genetic improvement, little has been achieved so far, partly due to the lack of genetic information coupled with the complex relationship between protein content and grain yield. This review reports on the current knowledge on Vicia faba seed storage proteins, their structure, composition, and genetic control, and highlights key areas for further improvement of the content and composition of Vicia faba seed storage proteins on the basis of recent advances in Vicia faba genome knowledge and genetic tools.
    MeSH term(s) Genetic Variation ; Seed Storage Proteins/chemistry ; Seed Storage Proteins/genetics ; Seed Storage Proteins/metabolism ; Seeds/chemistry ; Seeds/genetics ; Seeds/metabolism ; Vicia faba/chemistry ; Vicia faba/genetics ; Vicia faba/metabolism
    Chemical Substances Seed Storage Proteins
    Language English
    Publishing date 2018-11-16
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.8b04992
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1172: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article: Identification and Quantification of Major Faba Bean Seed Proteins

    Warsame, Ahmed O / Michael, Nicholas / O’Sullivan, Donal M / Tosi, Paola

    Journal of agricultural and food chemistry. 2020 July 17, v. 68, no. 32

    2020  

    Abstract: Faba bean (Vicia faba L.) holds great importance for human and animal nutrition for its high protein content. However, better understanding of its seed protein composition is required in order to develop cultivars that meet market demands for plant ... ...

    Abstract Faba bean (Vicia faba L.) holds great importance for human and animal nutrition for its high protein content. However, better understanding of its seed protein composition is required in order to develop cultivars that meet market demands for plant proteins with specific quality attributes. In this study, we screened 35 diverse Vicia faba genotypes by employing the one-dimensional sodium dodecyl sulfate–polyacrylamide gel electrophoresis (1D SDS-PAGE) method, and 35 major protein bands obtained from three genotypes with contrasting seed protein profiles were further analyzed by mass spectrometry (MS). Twenty-five of these protein bands (MW range: ∼ 9–107 kDa) had significant (p ≤ 0.05) matches to polypeptides in protein databases. MS analysis showed that most of the analyzed protein bands contained more than one protein type and, in total, over 100 proteins were identified. These included major seed storage proteins such as legumin, vicilin, and convicilin, as well as other protein classes like lipoxygenase, heat shock proteins, sucrose-binding proteins, albumin, and defensin. Furthermore, seed protein extracts were separated by size-exclusion high-performance liquid chromatography (SE-HPLC), and percentages of the major protein classes were determined. On average, legumin and vicilin/convicilin accounted for 50 and 27% of the total protein extract, respectively. However, the proportions of these proteins varied considerably among genotypes, with the ratio of legumin:vicilin/convicilin ranging from 1:1 to 1:3. In addition, there was a significant (p < 0.01) negative correlation between the contents of these major fractions (r = −0.83). This study significantly extends the number of identified Vicia faba seed proteins and reveals new qualitative and quantitative variation in seed protein composition, filling a significant gap in the literature. Moreover, the germplasm and screening methods presented here are expected to contribute in selecting varieties with improved protein content and quality.
    Keywords Vicia faba ; albumins ; animal nutrition ; convicilin ; cultivars ; faba beans ; food chemistry ; germplasm ; heat stress ; high performance liquid chromatography ; humans ; legumin ; lipoxygenases ; markets ; mass spectrometry ; polyacrylamide gel electrophoresis ; polypeptides ; protein content ; seed storage ; vicilin
    Language English
    Dates of publication 2020-0717
    Size p. 8535-8544.
    Publishing place American Chemical Society
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.0c02927
    Database NAL-Catalogue (AGRICOLA)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 1185: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  6. Article: Seed Storage Proteins of Faba Bean (Vicia faba L): Current Status and Prospects for Genetic Improvement

    Warsame, Ahmed O / Donal M. O’Sullivan / Paola Tosi

    Journal of agricultural and food chemistry. 2018 Nov. 07, v. 66, no. 48

    2018  

    Abstract: Faba bean (Vicia faba L.) is one of the foremost candidate crops for simultaneously increasing both sustainability and global supply of plant protein. On a dry matter basis, its seeds contain about 29% protein of which more than 80% consists of globulin ... ...

    Abstract Faba bean (Vicia faba L.) is one of the foremost candidate crops for simultaneously increasing both sustainability and global supply of plant protein. On a dry matter basis, its seeds contain about 29% protein of which more than 80% consists of globulin storage proteins (vicilin and legumin). However, to achieve optimum utilization of this crop for human and animal nutrition, both protein content and quality have to be improved. Though initial investigations on the heritability of these traits indicated the possibility for genetic improvement, little has been achieved so far, partly due to the lack of genetic information coupled with the complex relationship between protein content and grain yield. This review reports on the current knowledge on Vicia faba seed storage proteins, their structure, composition, and genetic control, and highlights key areas for further improvement of the content and composition of Vicia faba seed storage proteins on the basis of recent advances in Vicia faba genome knowledge and genetic tools.
    Keywords animal nutrition ; crops ; faba beans ; genetic improvement ; genome ; globulins ; grain yield ; heritability ; legumin ; protein content ; seed storage proteins ; seeds ; Vicia faba ; vicilin
    Language English
    Dates of publication 2018-1107
    Size p. 12617-12626.
    Publishing place American Chemical Society
    Document type Article
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.8b04992
    Database NAL-Catalogue (AGRICOLA)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 1185: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  7. Article ; Online: The giant diploid faba genome unlocks variation in a global protein crop.

    Jayakodi, Murukarthick / Golicz, Agnieszka A / Kreplak, Jonathan / Fechete, Lavinia I / Angra, Deepti / Bednář, Petr / Bornhofen, Elesandro / Zhang, Hailin / Boussageon, Raphaël / Kaur, Sukhjiwan / Cheung, Kwok / Čížková, Jana / Gundlach, Heidrun / Hallab, Asis / Imbert, Baptiste / Keeble-Gagnère, Gabriel / Koblížková, Andrea / Kobrlová, Lucie / Krejčí, Petra /
    Mouritzen, Troels W / Neumann, Pavel / Nadzieja, Marcin / Nielsen, Linda Kærgaard / Novák, Petr / Orabi, Jihad / Padmarasu, Sudharsan / Robertson-Shersby-Harvie, Tom / Robledillo, Laura Ávila / Schiemann, Andrea / Tanskanen, Jaakko / Törönen, Petri / Warsame, Ahmed O / Wittenberg, Alexander H J / Himmelbach, Axel / Aubert, Grégoire / Courty, Pierre-Emmanuel / Doležel, Jaroslav / Holm, Liisa U / Janss, Luc L / Khazaei, Hamid / Macas, Jiří / Mascher, Martin / Smýkal, Petr / Snowdon, Rod J / Stein, Nils / Stoddard, Frederick L / Stougaard, Jens / Tayeh, Nadim / Torres, Ana M / Usadel, Björn / Schubert, Ingo / O'Sullivan, Donal Martin / Schulman, Alan H / Andersen, Stig Uggerhøj

    Nature

    2023  Volume 615, Issue 7953, Page(s) 652–659

    Abstract: Increasing the proportion of locally produced plant protein in currently meat-rich diets could substantially reduce greenhouse gas emissions and loss of ... ...

    Abstract Increasing the proportion of locally produced plant protein in currently meat-rich diets could substantially reduce greenhouse gas emissions and loss of biodiversity
    MeSH term(s) Chromosomes, Plant/genetics ; Crops, Agricultural/genetics ; Crops, Agricultural/metabolism ; Diploidy ; DNA Copy Number Variations/genetics ; DNA, Satellite/genetics ; Gene Amplification/genetics ; Genes, Plant/genetics ; Genetic Variation/genetics ; Genome, Plant/genetics ; Genome-Wide Association Study ; Genomics ; Geography ; Plant Breeding/methods ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Recombination, Genetic ; Retroelements/genetics ; Seeds/anatomy & histology ; Seeds/genetics ; Vicia faba/anatomy & histology ; Vicia faba/genetics ; Vicia faba/metabolism
    Chemical Substances DNA, Satellite ; Plant Proteins ; Retroelements
    Language English
    Publishing date 2023-03-08
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 120714-3
    ISSN 1476-4687 ; 0028-0836
    ISSN (online) 1476-4687
    ISSN 0028-0836
    DOI 10.1038/s41586-023-05791-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 26: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 9: Show issues
    Zs.MO 244: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top