Article ; Online: Differential LysoTracker Uptake Defines Two Populations of Distal Epithelial Cells in Idiopathic Pulmonary Fibrosis.
2022 Volume 11, Issue 2
Abstract: Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal degenerative lung disease of unknown etiology. Although in its final stages it implicates, in a reactive manner, all lung cell types, the initial damage involves the alveolar epithelial ... ...
| Abstract | Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal degenerative lung disease of unknown etiology. Although in its final stages it implicates, in a reactive manner, all lung cell types, the initial damage involves the alveolar epithelial compartment, in particular the alveolar epithelial type 2 cells (AEC2s). AEC2s serve dual progenitor and surfactant secreting functions, both of which are deeply impacted in IPF. Thus, we hypothesize that the size of the surfactant processing compartment, as measured by LysoTracker incorporation, allows the identification of different epithelial states in the IPF lung. Flow cytometry analysis of epithelial LysoTracker incorporation delineates two populations (Lyso |
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| MeSH term(s) | Alveolar Epithelial Cells/metabolism ; Amines/metabolism ; Animals ; Biomarkers/metabolism ; Bleomycin ; CD24 Antigen/metabolism ; Epithelium/pathology ; Gene Expression Profiling ; Humans ; Idiopathic Pulmonary Fibrosis/genetics ; Idiopathic Pulmonary Fibrosis/pathology ; Keratin-5/metabolism ; Mice, Inbred C57BL ; Pulmonary Surfactant-Associated Proteins/metabolism ; Receptors, Nerve Growth Factor/metabolism ; Tissue Donors ; Transcription, Genetic ; Up-Regulation ; Mice |
| Chemical Substances | Amines ; Biomarkers ; CD24 Antigen ; Keratin-5 ; Pulmonary Surfactant-Associated Proteins ; Receptors, Nerve Growth Factor ; Red DND-99 ; Bleomycin (11056-06-7) |
| Language | English |
| Publishing date | 2022-01-11 |
| Publishing country | Switzerland |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2661518-6 |
| ISSN | 2073-4409 ; 2073-4409 |
| ISSN (online) | 2073-4409 |
| ISSN | 2073-4409 |
| DOI | 10.3390/cells11020235 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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