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Article ; Online: Troubleshooting in thoracoscopic anatomical lung resection for lung cancer.

Watanabe, Atsushi

2020 Volume 51, Issue 5, Page(s) 669–677

Abstract: Video-assisted thoracoscopic surgery (VATS) anatomical lung resection (ALR) has been gaining popularity in the treatment of lung cancer in line with remarkable advances in both equipment and technique. The development and refinement of its technique have ...

Abstract	Video-assisted thoracoscopic surgery (VATS) anatomical lung resection (ALR) has been gaining popularity in the treatment of lung cancer in line with remarkable advances in both equipment and technique. The development and refinement of its technique have allowed thoracic surgeons to perform a wide variety of challenging and complex procedures in a minimally invasive fashion. Careful and meticulous preparation may shift in the future with the increasing sophistication and capabilities of VATS ALR. Moreover, constant awareness and a structured plan of the procedure are imperative to reducing or preventing complications. Intraoperative major complications during VATS ALR are infrequent, but can have catastrophic consequences. The decision to continue with VATS should take into consideration the surgeon's skill level and ease with the approach and the relative potential benefit against the risk to the patient. We conducted this study to investigate the possible problems during VATS ALR and identify how to solve them based on the previous literature and our institutional data sampling.
Language	English
Publishing date	2020-09-17
Publishing country	Japan
Document type	Journal Article ; Review
ZDB-ID	1115435-4
ISSN	1436-2813 ; 0941-1291
ISSN (online)	1436-2813
ISSN	0941-1291
DOI	10.1007/s00595-020-02136-x
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Article ; Online: The quantification of the airborne plastic particles of 0.43-11 μm: Procedure development and application to atmospheric environment.

Morioka, Tamaki / Tanaka, Shuhei / Kohama-Inoue, Akiko / Watanabe, Atsushi

Chemosphere

2024 Volume 351, Page(s) 141131

Abstract: The environmental degradation of microplastics results in ultrafine particles that may incur severe biological concerns. Despite this, the atmospheric existence of plastics of less than a few microns has barely been investigated due to the particle size ... ...

Abstract	The environmental degradation of microplastics results in ultrafine particles that may incur severe biological concerns. Despite this, the atmospheric existence of plastics of less than a few microns has barely been investigated due to the particle size limit of conventional analytical methods. This study develops a procedure to quantify and characterize plastic particles (including nanoplastics; less than 1 μm) in the air through fractional sampling, a simple pretreatment method, and pyrolysis-gas chromatography-mass spectrometry (pyr-GC/MS). We targeted 11 major polymers, namely, polyethylene, polypropylene, polystyrene, acrylonitrile-butadiene-styrene resin, styrene-butadiene rubber, polymethylmethacrylate, polycarbonate, polyvinyl chloride, polyethylene terephthalate (PET), polyamide 6, and polyamide 66 (PA66). The average spike and recovery rate of each polymer in the aerosol collected on the roof of a four-story building near a major road in Kyoto, Japan, amounted to 78-130%, with a coefficient of variation of less than 15%. By coupling pyr-GC/MS analysis with fractional sampling of particles within the size range of >11 μm, 11-7.0 μm, 7.0-4.7 μm, 4.7-3.3 μm, 3.3-2.1 μm, 2.1-1.1 μm, 1.1-0.65 μm, 0.65-0.43 μm, it was possible to quantify airborne nano- and microplastics by particle size. Polyethylene, polystyrene, PET, and PA66 were detected in the air, and the total mass concentration of tiny plastic particles (0.43-11 μm) amounted to 1.20 μg/m
MeSH term(s)	Plastics/analysis ; Microplastics/analysis ; Polystyrenes/analysis ; Water Pollutants, Chemical/analysis ; Polyethylenes ; Environmental Monitoring/methods
Chemical Substances	Plastics ; Microplastics ; Polystyrenes ; Water Pollutants, Chemical ; Polyethylenes
Language	English
Publishing date	2024-01-06
Publishing country	England
Document type	Journal Article
ZDB-ID	120089-6
ISSN	1879-1298 ; 0045-6535 ; 0366-7111
ISSN (online)	1879-1298
ISSN	0045-6535 ; 0366-7111
DOI	10.1016/j.chemosphere.2024.141131
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Article ; Online: Utility of chloroplast DNA haplotype data for ecological restoration using Fagus crenata seedlings in case of incomplete seed source information availability

Takahashi, Makoto / Goto, Susumu / Fukuda, Yoko / Watanabe, Atsushi

Ecological Research. 2023 Mar., v. 38, no. 2 p.255-264

2023

Abstract: Forest trees species are often genetically adapted to local environmental conditions. Therefore, local seeds are recommended for ecological restoration. However, seedlings of broad‐leaved tree species, such as Japanese beech (Fagus crenata), are limited ... ...

Abstract	Forest trees species are often genetically adapted to local environmental conditions. Therefore, local seeds are recommended for ecological restoration. However, seedlings of broad‐leaved tree species, such as Japanese beech (Fagus crenata), are limited in their commercial seedling production in Japan. Thus, long‐distance transfer of seeds and/or seedlings is common. F. crenata's distinct geographical structure is well known; large‐scale seed transfer may increase the risk of genetic disturbance. Several provenance trials of the species revealed that phenotypic traits such as leaf area and bud flush date differed latitudinally and/or between the Pacific Ocean side and the Japan Sea side. We investigated leaf size and bud flush date and identified the chloroplast DNA haplotype of trees planted in two provenance trials established in Hokkaido, Japan. We then examined whether cpDNA haplotype information is useful as a proxy in ecological restoration when using seedlings with incomplete seed source information. This study indicated that suitable seedlings could be selected based on chloroplast DNA haplotype information in F. crenata in the case of incomplete seed source information.
Keywords	Fagus crenata ; Japan ; broadleaved trees ; chloroplast DNA ; ecological restoration ; forests ; haplotypes ; leaf area ; phenotype ; provenance ; research ; risk ; seedling production ; Pacific Ocean
Language	English
Dates of publication	2023-03
Size	p. 255-264.
Publishing place	John Wiley & Sons, Inc.
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	233459-8
ISSN	1440-1703 ; 0912-3814
ISSN (online)	1440-1703
ISSN	0912-3814
DOI	10.1111/1440-1703.12351
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Article ; Online: Pulmonary artery occlusion post immunotherapy for hilar node-positive lung cancer.

Tada, Makoto / Uehara, Hirofumi / Ohyu, Takeshi / Watanabe, Atsushi

Thoracic cancer

2023 Volume 14, Issue 22, Page(s) 2222–2224

Abstract: We present the case of a patient who developed pulmonary artery occlusion following immune-checkpoint inhibitor (ICI) therapy for non-small cell lung cancer. A 69-year-old man with squamous cell carcinoma (yc-T1cN0M0), initially diagnosed at c-stage IVA ( ...

Abstract	We present the case of a patient who developed pulmonary artery occlusion following immune-checkpoint inhibitor (ICI) therapy for non-small cell lung cancer. A 69-year-old man with squamous cell carcinoma (yc-T1cN0M0), initially diagnosed at c-stage IVA (T3N1M1b), in the upper lobe of the left lung, was scheduled to undergo salvage lung resection after ICI therapy. However, he exhibited an occlusion of the lingular pulmonary artery near the clinically metastatic hilar lymph node. To avoid severe adhesions, the patient underwent successful wedge resection without dividing pulmonary vessels and was discharged without complications. Surgeons should be prepared for pulmonary artery changes following ICI therapy.
MeSH term(s)	Male ; Humans ; Aged ; Lung Neoplasms/pathology ; Carcinoma, Non-Small-Cell Lung/pathology ; Pulmonary Artery/pathology ; Lung/pathology ; Immunotherapy/adverse effects
Language	English
Publishing date	2023-06-20
Publishing country	Singapore
Document type	Case Reports
ZDB-ID	2625856-0
ISSN	1759-7714 ; 1759-7706
ISSN (online)	1759-7714
ISSN	1759-7706
DOI	10.1111/1759-7714.15001
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Article ; Online: Bovine neutrophils stimulated with Streptococcus uberis induce neutrophil extracellular traps, and cause cytotoxicity and transcriptional upregulation of inflammatory cytokine genes in bovine mammary epithelial cells.

Goto, Shinya / Mikami, Osamu / Nagasawa, Yuya / Watanabe, Atsushi

The Journal of veterinary medical science

2023 Volume 86, Issue 2, Page(s) 141–149

Abstract: This study aimed to understand the response of neutrophils stimulated by Streptococcus uberis, a major cause of mastitis. It was found that the production of neutrophil extracellular traps (NETs) was induced in milk clots from mastitic milk produced by S. ...

Abstract	This study aimed to understand the response of neutrophils stimulated by Streptococcus uberis, a major cause of mastitis. It was found that the production of neutrophil extracellular traps (NETs) was induced in milk clots from mastitic milk produced by S. uberis-infected bovine udders. The release of NETs from neutrophils stimulated by S. uberis was investigated. Bovine neutrophils cocultured with S. uberis in vitro released the components of NETs, which contained extracellular DNA and elastase. Bovine mammary epithelial cells (BMECs) incubated in coculture supernatants containing components of NETs, caused cytotoxicity and transcriptional upregulation of inflammatory cytokines, including of interleukin (IL) -1β, tumor necrosis factor (TNF)-α, IL-6, and IL-8, in BMECs. These findings suggest that bovine neutrophils stimulated by S. uberis induce responses that cause exacerbated inflammation, such as NET formation, cytotoxicity against BMECs, and increased production of inflammatory cytokines. Bovine neutrophil responses stimulated by S. uberis could be involved in the progression of S. uberis-induced mastitis.
MeSH term(s)	Female ; Animals ; Cattle ; Neutrophils/metabolism ; Extracellular Traps/metabolism ; Streptococcal Infections/veterinary ; Streptococcal Infections/pathology ; Up-Regulation ; Mastitis, Bovine ; Cytokines/genetics ; Tumor Necrosis Factor-alpha/genetics ; Mammary Glands, Animal/pathology ; Interleukin-1beta ; Epithelial Cells/metabolism ; Cattle Diseases/pathology ; Streptococcus
Chemical Substances	Cytokines ; Tumor Necrosis Factor-alpha ; Interleukin-1beta
Language	English
Publishing date	2023-12-18
Publishing country	Japan
Document type	Journal Article
ZDB-ID	1071753-5
ISSN	1347-7439 ; 0916-7250
ISSN (online)	1347-7439
ISSN	0916-7250
DOI	10.1292/jvms.23-0302
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Article ; Online: Comparison of imaging changes in pulmonary artery diameter at the occlusion site using silk or metal clamps for pulmonary artery troubles.

Chiba, Yoshiki / Miyajima, Masahiro / Takahashi, Yuki / Shindo, Yuma / Tsuruta, Kodai / Maki, Ryunosuke / Watanabe, Atsushi

European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery

2024 Volume 65, Issue 3

Abstract: Objectives: We analysed our clinical experience using silk sutures [the double-loop technique (DLT)] or DeBakey type vascular clamp (DeBakey clamp) for pulmonary artery (PA) troubles during anatomical lung resection to validate its practicality and ... ...

Abstract	Objectives: We analysed our clinical experience using silk sutures [the double-loop technique (DLT)] or DeBakey type vascular clamp (DeBakey clamp) for pulmonary artery (PA) troubles during anatomical lung resection to validate its practicality and safety. Methods: We retrospectively reviewed the records of patients who underwent either of the above clamping techniques during anatomical lung resection at our hospital between April 2007 and August 2022. We measured the PA diameter at the occlusion site on computed tomography images acquired within 1 year pre- and postoperatively. The difference between pre- and postoperative diameters of the occlusion sites was calculated as the change in the PA diameter. We zoned the occlusion site of the PA to adjust for variation. PA deformation was evaluated as an adverse event caused by clamping. Results: Ultimately, 27 and 26 patients who underwent the DLT and DeBakey clamp, respectively, were included. No additional injury due to the clamp procedure was found in either group. For zone R1/L1, defined as the main PA, the median changes in the PA diameter were 0.02 (-0.7 to 0.27) mm for the DLT and 0.36 (-0.28 to 0.89) mm for the DeBakey clamp. No significant differences were observed between the 2 groups (P = 0.106). Furthermore, no aneurysms, dissections, or stenoses were found in either group. Conclusions: The DLT and DeBakey clamp had only minimal effects on the occlusion site of the PA. The DLT is a practical thoracoscopic technique for PA bleeding when primary haemostasis has been achieved.
MeSH term(s)	Humans ; Pulmonary Artery/diagnostic imaging ; Pulmonary Artery/surgery ; Silk ; Retrospective Studies ; Tomography, X-Ray Computed ; Thoracic Surgery, Video-Assisted/methods
Chemical Substances	Silk
Language	English
Publishing date	2024-02-07
Publishing country	Germany
Document type	Journal Article
ZDB-ID	639293-3
ISSN	1873-734X ; 1010-7940 ; 1567-4258
ISSN (online)	1873-734X
ISSN	1010-7940 ; 1567-4258
DOI	10.1093/ejcts/ezae034
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Article ; Online: Genetic Counseling and Genetic Testing for Familial Hypercholesterolemia.

Tada, Hayato / Kawashiri, Masa-Aki / Nohara, Atsushi / Sekiya, Tomoko / Watanabe, Atsushi / Takamura, Masayuki

Genes

2024 Volume 15, Issue 3

Abstract: Familial hypercholesterolemia (FH) is one of the most common autosomal codominant Mendelian diseases. The major complications of FH include tendon and cutaneous xanthomas and coronary artery disease (CAD) associated with a substantial elevation of serum ... ...

Abstract	Familial hypercholesterolemia (FH) is one of the most common autosomal codominant Mendelian diseases. The major complications of FH include tendon and cutaneous xanthomas and coronary artery disease (CAD) associated with a substantial elevation of serum low-density lipoprotein levels (LDL). Genetic counseling and genetic testing for FH is useful for its diagnosis, risk stratification, and motivation for further LDL-lowering treatments. In this study, we summarize the epidemiology of FH based on numerous genetic studies, including its pathogenic variants, genotype-phenotype correlation, prognostic factors, screening, and usefulness of genetic counseling and genetic testing. Due to the variety of treatments available for this common Mendelian disease, genetic counseling and genetic testing for FH should be implemented in daily clinical practice.
MeSH term(s)	Humans ; Cholesterol, LDL/genetics ; Genetic Counseling ; Hyperlipoproteinemia Type II/diagnosis ; Hyperlipoproteinemia Type II/genetics ; Genetic Testing ; Coronary Artery Disease/genetics
Chemical Substances	Cholesterol, LDL
Language	English
Publishing date	2024-02-26
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2527218-4
ISSN	2073-4425 ; 2073-4425
ISSN (online)	2073-4425
ISSN	2073-4425
DOI	10.3390/genes15030297
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Article ; Online: Robotic single right anterior medial (S3b) subsegmentectomy for early primary lung cancer in a patient with a small physique.

Takase, Yoshiaki / Miyajima, Masahiro / Watanabe, Atsushi

Multimedia manual of cardiothoracic surgery : MMCTS

2022 Volume 2022

Abstract: Subsegmentectomy is an optional treatment for early primary lung cancer presenting without adequate surgical margins for wedge resection. There are no previous reports regarding a robotic single subsegmentectomy. We report a robotic single ... ...

Abstract	Subsegmentectomy is an optional treatment for early primary lung cancer presenting without adequate surgical margins for wedge resection. There are no previous reports regarding a robotic single subsegmentectomy. We report a robotic single subsegmentectomy of the right anterior medial subsegment (S3b). The lung parenchyma was dissected with a Vessel Sealer Extend along the intersubsegmental veins for proper lung mobilization to solve the directional limitations of the robotic stapler. Additionally, we inserted the most ventral port from the subcostal region to increase the distance between the ports. Performing a robotic single subsegmentectomy is challenging. However, our techniques help to achieve a safe and effective operation.
MeSH term(s)	Humans ; Lung ; Lung Neoplasms/surgery ; Robotic Surgical Procedures
Language	English
Publishing date	2022-05-25
Publishing country	England
Document type	Video-Audio Media
ZDB-ID	2280156-X
ISSN	1813-9175 ; 1813-9175
ISSN (online)	1813-9175
ISSN	1813-9175
DOI	10.1510/mmcts.2022.027
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Article: [Companion Diagnostics for Solid Tumors].

Watanabe, Atsushi

Rinsho byori. The Japanese journal of clinical pathology

2015 Volume 63, Issue 11, Page(s) 1310–1315

Abstract: Companion diagnostics (CoDx) will likely continue to rapidly increase in number and application to disease areas including solid tumors, for example EGFR for gefitinib and ALK fusion gene for crizotinib in non-small-cell lung cancer; KRAS against the use ...

Abstract	Companion diagnostics (CoDx) will likely continue to rapidly increase in number and application to disease areas including solid tumors, for example EGFR for gefitinib and ALK fusion gene for crizotinib in non-small-cell lung cancer; KRAS against the use of cetuximab and panitumumab in colorectal cancer; HER2 for trastuzumab in breast cancer. CoDx are an indispensable part of personalized medicine and pharmacogenomics. In CoDx development, there are still many challenges, such as the business model promoting cooperation between diagnostics and pharmaceutical companies, and also the regulations related to CoDx. The FDA notice on the development of CoDx in 2011 recommended the co-development of a new drug and CoDx as the best practice, and the Ministry of Health, Labour and Welfare in Japan also issued a statement in 2013. In addition, the recent discovery of many novel variants in the DNA sequence, advances in sequencing and genomic technology, and improved analytic methods have enabled the impact of germline and somatic mutations to be determined using multiplex diagnosis. The complex challenges to develop CoDx necessitate a close collaboration among academic institutions, regulatory authorities, and pharmaceutical companies. [Review].
MeSH term(s)	Biomarkers, Tumor ; Breast Neoplasms/diagnosis ; Breast Neoplasms/drug therapy ; Breast Neoplasms/genetics ; Cetuximab/therapeutic use ; Colonic Neoplasms/diagnosis ; Colonic Neoplasms/drug therapy ; Colonic Neoplasms/genetics ; Diagnostic Uses of Chemicals ; Drug Discovery ; Gene Fusion/genetics ; Guidelines as Topic ; Humans ; Lung Neoplasms/diagnosis ; Lung Neoplasms/drug therapy ; Lung Neoplasms/genetics ; Molecular Diagnostic Techniques ; Molecular Targeted Therapy ; Pharmacogenetics ; Precision Medicine ; Proto-Oncogene Proteins p21(ras)/genetics ; Pyrazoles/therapeutic use ; Pyridines/therapeutic use ; Quinazolines/therapeutic use ; Receptor Protein-Tyrosine Kinases/genetics ; Receptor, Epidermal Growth Factor ; Receptor, ErbB-2/genetics ; Trastuzumab/therapeutic use
Chemical Substances	Biomarkers, Tumor ; Diagnostic Uses of Chemicals ; KRAS protein, human ; Pyrazoles ; Pyridines ; Quinazolines ; crizotinib (53AH36668S) ; ERBB2 protein, human (EC 2.7.10.1) ; Receptor Protein-Tyrosine Kinases (EC 2.7.10.1) ; Receptor, Epidermal Growth Factor (EC 2.7.10.1) ; Receptor, ErbB-2 (EC 2.7.10.1) ; anaplastic lymphoma kinase (EC 2.7.10.1) ; Proto-Oncogene Proteins p21(ras) (EC 3.6.5.2) ; Trastuzumab (P188ANX8CK) ; Cetuximab (PQX0D8J21J) ; gefitinib (S65743JHBS)
Language	Japanese
Publishing date	2015-11
Publishing country	Japan
Document type	English Abstract ; Journal Article ; Review
ZDB-ID	604196-6
ISSN	0047-1860 ; 0485-1404
ISSN	0047-1860 ; 0485-1404
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Article: Intensity-Modulated Radiation Therapy for Bilateral Choroidal Metastases Involving Macula and Optic Disc.

Kubo, Kenya / Hashida, Noriyasu / Watanabe, Atsushi / Maruyama, Kazuichi / Oh, Ryoong-Jin / Nishida, Kohji

Cureus

2023 Volume 15, Issue 10, Page(s) e46729

Abstract: This case report discusses the case of a 76-year-old woman with choroidal metastasis from breast cancer who was treated with intensity-modulated radiation therapy (IMRT). Choroidal metastasis is a common ocular tumor, and the occurrence of this condition ...

Abstract	This case report discusses the case of a 76-year-old woman with choroidal metastasis from breast cancer who was treated with intensity-modulated radiation therapy (IMRT). Choroidal metastasis is a common ocular tumor, and the occurrence of this condition has increased due to improved diagnostic tools and longer survival of metastatic patients. IMRT is an innovative radiation therapy technique that reduces complications and improves the curative effect by concentrating radiation on the tumor while minimizing exposure to surrounding tissues. In this case, the patient had a history of breast cancer and was undergoing chemotherapy when she presented with vision loss and blurred vision. Imaging tests confirmed choroidal metastasis, and IMRT was performed under the guidance of a radiation oncologist. After treatment, the choroidal lesion dramatically reduced in size, and the patient's vision improved. The text concludes that radiation therapy, including IMRT, is becoming more common as a treatment for ocular metastasis to improve vision and preserve the eye. When choosing radiation therapy, it is essential to consider the size of the tumor and the impact on surrounding tissues. IMRT is an effective treatment that enables precise and concentrated irradiation of the tumor tissue while minimizing exposure to normal tissues.
Language	English
Publishing date	2023-10-09
Publishing country	United States
Document type	Case Reports
ZDB-ID	2747273-5
ISSN	2168-8184
ISSN	2168-8184
DOI	10.7759/cureus.46729
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