LIVIVO - Search results -

Search results

Result 1 - 10 of total 18

Search options

Article ; Online: Cell adhesion control by photoinduced LCST shift of PNIPAAm-based brush scaffolds.

Imato, Keiichi / Nagata, Kazuho / Watanabe, Rina / Takeda, Naoya

2020 Volume 8, Issue 12, Page(s) 2393–2399

Abstract: Poly(N-isopropylacrylamide) (PNIPAAm)-brush substrates are one of the most successful dynamic scaffolds for thermally controlling cell adhesion. Endowing substrates with photocontrollability promises further development and applications in the biomedical ...

Abstract	Poly(N-isopropylacrylamide) (PNIPAAm)-brush substrates are one of the most successful dynamic scaffolds for thermally controlling cell adhesion. Endowing substrates with photocontrollability promises further development and applications in the biomedical and bioengineering fields. In this study, we developed photoresponsive PNIPAAm copolymers by incorporation of spiropyran into the polymer chain, enabling the photoinduced shift of the lower critical solution temperatures. Their brush substrates were fabricated in a simple, facile grafting-to manner. Co-grafting with non-cell-adhesive poly(ethylene glycol) enhanced the modulation of surface chemical properties upon photostimulation; it enabled significant photocontrol of cell adhesion of several types with keeping culture temperature constant.
MeSH term(s)	Acrylic Resins/chemistry ; Acrylic Resins/pharmacology ; Animals ; Cell Adhesion/drug effects ; Cell Line ; Mice ; NIH 3T3 Cells ; Particle Size ; Photochemical Processes ; Solutions ; Surface Properties ; Temperature ; Ultraviolet Rays
Chemical Substances	Acrylic Resins ; Solutions ; poly-N-isopropylacrylamide (25189-55-3)
Language	English
Publishing date	2020-02-27
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2702241-9
ISSN	2050-7518 ; 2050-750X
ISSN (online)	2050-7518
ISSN	2050-750X
DOI	10.1039/c9tb02958c
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article: Cell adhesion control by photoinduced LCST shift of PNIPAAm-based brush scaffolds

Imato, Keiichi / Nagata, Kazuho / Watanabe, Rina / Takeda, Naoya

Journal of materials chemistry B. 2020 Mar. 25, v. 8, no. 12

2020

Abstract	Poly(N-isopropylacrylamide) (PNIPAAm)-brush substrates are one of the most successful dynamic scaffolds for thermally controlling cell adhesion. Endowing substrates with photocontrollability promises further development and applications in the biomedical and bioengineering fields. In this study, we developed photoresponsive PNIPAAm copolymers by incorporation of spiropyran into the polymer chain, enabling the photoinduced shift of the lower critical solution temperatures. Their brush substrates were fabricated in a simple, facile grafting-to manner. Co-grafting with non-cell-adhesive poly(ethylene glycol) enhanced the modulation of surface chemical properties upon photostimulation; it enabled significant photocontrol of cell adhesion of several types with keeping culture temperature constant.
Keywords	bioengineering ; cell adhesion ; composite polymers ; physicochemical properties ; polyethylene glycol ; temperature
Language	English
Dates of publication	2020-0325
Size	p. 2393-2399.
Publishing place	The Royal Society of Chemistry
Document type	Article
ZDB-ID	2702241-9
ISSN	2050-7518 ; 2050-750X
ISSN (online)	2050-7518
ISSN	2050-750X
DOI	10.1039/c9tb02958c
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: Distribution of cervical intraepithelial neoplasia is closely associated with HPV status and uterine position.

Tamura, Daisuke / Sako, Wataru / Watanabe, Rina / Shitara, Akihiro / Saito, Fumiko / Yamauchi, Misa / Sugita, Akihiro / Karube, Akihiro

Journal of medical virology

2023 Volume 95, Issue 5, Page(s) e28777

Abstract: Although cervical intraepithelial neoplasia (CIN) lesions are considered to be not randomly distributed across the cervix, but predominantly in the anterior wall, the clinicopathological etiology remains unknown. Herein, we aimed to elucidate the ... ...

Abstract	Although cervical intraepithelial neoplasia (CIN) lesions are considered to be not randomly distributed across the cervix, but predominantly in the anterior wall, the clinicopathological etiology remains unknown. Herein, we aimed to elucidate the relationship between quantitatively measured area of CIN2/3 and cervical cancer associated factors by retrospective cohort study. We analyzed 235 consecutive therapeutic conization specimens dissected as a single intact section to determine CIN2/3 area and its correlation with both clinical risk factors including human papillomavirus (HPV) status (single or multiple infection) and uterine position defined by transvaginal ultrasound. Cervical wall was classified into three groups: anterior: (11, 12, 1, and 2 o'clock), posterior (5, 6, 7, and 8 o'clock) and lateral (3, 4, 9, and 10 o'clock). Multiple regression revealed that younger age and HPV16 status were significantly correlated with CIN2/3 area (p = 0.0224 and p = 0.0075, respectively). The Jonckheere-Terpstra test showed a significant trend: CIN2/3 area was highest in the single HPV16 group, followed by the multiple HPV16 group and the non-HPV16 group (p < 0.0001). CIN2/3 area in the anterior wall was statistically significantly larger than the posterior and lateral wall (p = 0.0059 and p = 0.0107, respectively). CIN2/3 area in the anterior wall was significantly greater with anteversion-anteflexion than retroversion-retroflexion (p = 0.0485), whereas CIN2/3 area in the posterior wall was significantly larger with retroversion-retroflexion than anteversion-anteflexion (p = 0.0394). In conclusion, the topographical distribution of CIN2/3 area is closely associated with patient age, high-risk HPV status, especially single HPV16 infection and uterine position.
MeSH term(s)	Female ; Humans ; Uterine Cervical Neoplasms ; Human Papillomavirus Viruses ; Retrospective Studies ; Uterine Cervical Dysplasia ; Cervix Uteri ; Human papillomavirus 16 ; Papillomavirus Infections ; Papillomaviridae/genetics
Language	English
Publishing date	2023-05-20
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	752392-0
ISSN	1096-9071 ; 0146-6615
ISSN (online)	1096-9071
ISSN	0146-6615
DOI	10.1002/jmv.28777
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1320: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: A liquid culture cancer spheroid model reveals low PI3K/Akt pathway activity and low adhesiveness to the extracellular matrix

Abe‐Fukasawa, Natsuki / Watanabe, Rina / Gen, Yuki / Nishino, Taito / Itasaki, Nobue

FEBS journal. 2021 Oct., v. 288, no. 19

2021

Abstract: Three‐dimensional (3D) cultures of cancer cells in liquid without extracellular matrix (ECM) offer in vitro models for metastasising conditions such as those in vessels and effusion. However, liquid culturing is often hindered by cell adhesiveness, which ...

Abstract	Three‐dimensional (3D) cultures of cancer cells in liquid without extracellular matrix (ECM) offer in vitro models for metastasising conditions such as those in vessels and effusion. However, liquid culturing is often hindered by cell adhesiveness, which causes large cell clumps. We previously described a liquid culture material, LA717, which prevents nonclonal cell adhesion and subsequent clumping, thus allowing clonal growth of spheroids in an anchorage‐independent manner. Here, we examined such liquid culture cancer spheroids for the acquisition of apical–basal polarity, sensitivity to an Akt inhibitor (anticancer drug MK‐2206) and interaction with ECM. The spheroids present apical plasma membrane on the surface, which originated from the failure of polarisation at the single‐cell stage and subsequent defects in phosphorylated ezrin accumulation at the cell boundary during the first cleavage, failing internal lumen formation. At the multicellular stage, liquid culture spheroids presented bleb‐like protrusion on the surface, which was enhanced by the activation of the PI3K/Akt pathway and reduced by PI3K/Akt inhibitors. Liquid culture spheroids exhibited slow proliferation speed and low endogenous pAkt levels compared with gel‐cultured spheroids and 2D‐cultured cells, explaining the susceptibility to the Akt‐inhibiting anticancer drug. Subcutaneous xenografting and in vitro analysis demonstrated low viability and adhesive property of liquid culture spheroids to ECM, while migratory and invasive capacities were comparable with gel‐cultured spheroids. These features agree with the low efficacy of circulating tumour spheroids in the settling step of metastasis. This study demonstrates the feature of anchorage‐independent spheroids and validates liquid cultures as a useful method in cancer spheroid research.
Keywords	adhesion ; antineoplastic agents ; cell adhesion ; extracellular matrix ; liquids ; metastasis ; migratory behavior ; models ; neoplasms ; plasma membrane ; viability ; xenotransplantation
Language	English
Dates of publication	2021-10
Size	p. 5650-5667.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	2173655-8
ISSN	1742-4658 ; 1742-464X
ISSN (online)	1742-4658
ISSN	1742-464X
DOI	10.1111/febs.15867
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 2006/704: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: A liquid culture cancer spheroid model reveals low PI3K/Akt pathway activity and low adhesiveness to the extracellular matrix.

Abe-Fukasawa, Natsuki / Watanabe, Rina / Gen, Yuki / Nishino, Taito / Itasaki, Nobue

The FEBS journal

2021 Volume 288, Issue 19, Page(s) 5650–5667

Abstract: Three-dimensional (3D) cultures of cancer cells in liquid without extracellular matrix (ECM) offer in vitro models for metastasising conditions such as those in vessels and effusion. However, liquid culturing is often hindered by cell adhesiveness, which ...

Abstract	Three-dimensional (3D) cultures of cancer cells in liquid without extracellular matrix (ECM) offer in vitro models for metastasising conditions such as those in vessels and effusion. However, liquid culturing is often hindered by cell adhesiveness, which causes large cell clumps. We previously described a liquid culture material, LA717, which prevents nonclonal cell adhesion and subsequent clumping, thus allowing clonal growth of spheroids in an anchorage-independent manner. Here, we examined such liquid culture cancer spheroids for the acquisition of apical-basal polarity, sensitivity to an Akt inhibitor (anticancer drug MK-2206) and interaction with ECM. The spheroids present apical plasma membrane on the surface, which originated from the failure of polarisation at the single-cell stage and subsequent defects in phosphorylated ezrin accumulation at the cell boundary during the first cleavage, failing internal lumen formation. At the multicellular stage, liquid culture spheroids presented bleb-like protrusion on the surface, which was enhanced by the activation of the PI3K/Akt pathway and reduced by PI3K/Akt inhibitors. Liquid culture spheroids exhibited slow proliferation speed and low endogenous pAkt levels compared with gel-cultured spheroids and 2D-cultured cells, explaining the susceptibility to the Akt-inhibiting anticancer drug. Subcutaneous xenografting and in vitro analysis demonstrated low viability and adhesive property of liquid culture spheroids to ECM, while migratory and invasive capacities were comparable with gel-cultured spheroids. These features agree with the low efficacy of circulating tumour spheroids in the settling step of metastasis. This study demonstrates the feature of anchorage-independent spheroids and validates liquid cultures as a useful method in cancer spheroid research.
MeSH term(s)	Animals ; Cell Adhesion/genetics ; Cell Culture Techniques ; Cell Line, Tumor ; Cell Polarity/genetics ; Extracellular Matrix/genetics ; Humans ; Mice ; Neoplasm Metastasis ; Neoplasms/genetics ; Neoplasms/pathology ; Oncogene Protein v-akt/genetics ; Phosphatidylinositol 3-Kinases/genetics ; Signal Transduction/genetics ; Spheroids, Cellular/metabolism ; Spheroids, Cellular/pathology ; Transplantation, Heterologous
Chemical Substances	Oncogene Protein v-akt (EC 2.7.11.1)
Language	English
Publishing date	2021-05-02
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2173655-8
ISSN	1742-4658 ; 1742-464X
ISSN (online)	1742-4658
ISSN	1742-464X
DOI	10.1111/febs.15867
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 260: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Perinatal Management in a Pregnant Woman with Ureteropelvic Junction Obstruction: Case Report and Literature Review.

Tamura, Daisuke / Narita, Shintaro / Yamauchi, Misa / Watanabe, Rina / Yokoyama, Shota / Kikuchi, Akane / Shitara, Akihiro / Chiba, Syuji / Saito, Fumiko / Sugita, Akihiro / Sato, Kazunari / Karube, Akihiro

Diagnostics (Basel, Switzerland)

2022 Volume 12, Issue 4

Abstract: Although giant hydronephrosis (GH) associated with ureteropelvic junction obstruction (UPJO) is extremely rarely detected in pregnant women, diagnostic methods, therapeutic approaches, and perinatal management have not been established. A 31-year-old ... ...

Abstract	Although giant hydronephrosis (GH) associated with ureteropelvic junction obstruction (UPJO) is extremely rarely detected in pregnant women, diagnostic methods, therapeutic approaches, and perinatal management have not been established. A 31-year-old Japanese primipara had a 15 cm × 12 cm multi-cystic mass in the right abdomen detected by transabdominal ultrasound at gestational week 26. Magnetic resonance imaging revealed that the mass was right renal GH. She underwent serial ultrasound-guided transretroperitoneal drainage as conservative treatment. She delivered vaginally at gestational week 36. Since she had flank pain and a documented non-functional right kidney, laparoscopic nephrectomy was conducted 22 months after delivery. UPJO with fewer smooth muscle cells and fibrosis was histologically diagnosed in the surgical specimen. Her postpartum and postoperative courses were uneventful for 10 months. We performed a literature review of diagnostic methods, clinical characteristics, and perinatal management in pregnant women with GH due to UPJO.
Language	English
Publishing date	2022-04-06
Publishing country	Switzerland
Document type	Case Reports
ZDB-ID	2662336-5
ISSN	2075-4418
ISSN	2075-4418
DOI	10.3390/diagnostics12040913
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Stressor interaction networks suggest antibiotic resistance co-opted from stress responses to temperature.

Cruz-Loya, Mauricio / Kang, Tina Manzhu / Lozano, Natalie Ann / Watanabe, Rina / Tekin, Elif / Damoiseaux, Robert / Savage, Van M / Yeh, Pamela J

The ISME journal

2018 Volume 13, Issue 1, Page(s) 12–23

Abstract: Environmental factors like temperature, pressure, and pH partly shaped the evolution of life. As life progressed, new stressors (e.g., poisons and antibiotics) arose as part of an arms race among organisms. Here we ask if cells co-opted existing ... ...

Abstract	Environmental factors like temperature, pressure, and pH partly shaped the evolution of life. As life progressed, new stressors (e.g., poisons and antibiotics) arose as part of an arms race among organisms. Here we ask if cells co-opted existing mechanisms to respond to new stressors, or whether new responses evolved de novo. We use a network-clustering approach based purely on phenotypic growth measurements and interactions among the effects of stressors on population growth. We apply this method to two types of stressors-temperature and antibiotics-to discover the extent to which their cellular responses overlap in Escherichia coli. Our clustering reveals that responses to low and high temperatures are clearly separated, and each is grouped with responses to antibiotics that have similar effects to cold or heat, respectively. As further support, we use a library of transcriptional fluorescent reporters to confirm heat-shock and cold-shock genes are induced by antibiotics. We also show strains evolved at high temperatures are more sensitive to antibiotics that mimic the effects of cold. Taken together, our results strongly suggest that temperature stress responses have been co-opted to deal with antibiotic stress.
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Biological Evolution ; Drug Resistance, Bacterial ; Escherichia coli/drug effects ; Escherichia coli/genetics ; Escherichia coli/physiology ; Gene Library ; Stress, Physiological ; Temperature
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2018-08-31
Publishing country	England
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2406536-5
ISSN	1751-7370 ; 1751-7362
ISSN (online)	1751-7370
ISSN	1751-7362
DOI	10.1038/s41396-018-0241-7
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Bonn / Germany

Z 7773: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Cyclosporine protects from intestinal epithelial injury by modulating butyrate uptake via upregulation of membrane monocarboxylate transporter 1 levels.

Ota, Shinji / Sakuraba, Hirotake / Hiraga, Hiroto / Yoshida, Shukuko / Satake, Miwa / Akemoto, Yui / Tanaka, Nahoko / Watanabe, Rina / Takato, Maeda / Murai, Yasuhisa / Ueno, Kayo / Niioka, Takenori / Hayakari, Makoto / Ishiguro, Yoh / Fukuda, Shinsaku

Biochemistry and biophysics reports

2020 Volume 24, Page(s) 100811

Abstract: Background and aims: A relationship between treatment outcomes and intestinal microbiota in patients with inflammatory bowel diseases has been demonstrated. Cyclosporine treatment leads to rapid improvement in severe ulcerative colitis. We hypothesized ... ...

Abstract	Background and aims: A relationship between treatment outcomes and intestinal microbiota in patients with inflammatory bowel diseases has been demonstrated. Cyclosporine treatment leads to rapid improvement in severe ulcerative colitis. We hypothesized that the potent effects of cyclosporine would be exerted through relationships between intestinal epithelial cells (IECs) and the host microbiota. The present study was designed to elucidate the effects of cyclosporine on monocarboxylate transporter 1 (MCT1) regulation and butyrate uptake by IECs. Methods: Colitis was induced in C57BL6 mice via the administration of 4% dextran sulfate sodium in drinking water, following which body weights, colon lengths, and histological scores were evaluated. To examine the role of butyrate in the protective effects of cyclosporine, MCT1 inhibitor and an antibiotic cocktail was administered and tributyrin (TB; a prodrug of butyrate) was supplemented; MCT1 protein expression and acetylated histone 3 (AcH3) signals in IECs, as well as the MCT1-membrane fraction of Caco-2 cells, were evaluated. To explore butyrate uptake, as s butyrate derivatives, 3-bromopyruvic acid (3-BrPA) and 1-pyrenebutyric acid were used. Results: Treatment with cyclosporine inhibited body weight loss and colon length shortening. However, treatment with MCT1 inhibitor and the antibiotic cocktail negated the efficacy of cyclosporine, whereas TB supplementation restored its protective effect. Furthermore, cyclosporine upregulated MCT1 expression in the membrane and the AcH3 signal in IECs, while also inducing higher anti-inflammatory cytokine production compared to that in the vehicle-treated mice. The transcription level of Conclusion: Cyclosporine treatment modulates butyrate uptake via the post-transcriptional upregulation of membrane MCT1 levels in IECs.
Language	English
Publishing date	2020-10-21
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2831046-9
ISSN	2405-5808 ; 2405-5808
ISSN (online)	2405-5808
ISSN	2405-5808
DOI	10.1016/j.bbrep.2020.100811
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Identification of a Novel Copy Number Variation of EYA4 Causing Autosomal Dominant Non-syndromic Hearing Loss.

Ishino, Takashi / Ogawa, Yui / Sonoyama, Toru / Taruya, Takayuki / Kono, Takashi / Hamamoto, Takao / Ueda, Tsutomu / Takeno, Sachio / Moteki, Hideaki / Nishio, Shin-Ya / Usami, Shin-Ichi / Nagano, Yuka / Yoshimura, Akiko / Yoshikawa, Kohei / Kato, Mikako / Ichimoto, Masaya / Watanabe, Rina

Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology

2021 Volume 42, Issue 7, Page(s) e866–e874

Abstract: Objective: Eyes absent 4 (EYA4) is the causative gene of autosomal dominant non-syndromic hereditary hearing loss, DFNA10. We aimed to identify a copy number variation of EYA4 in a non-syndromic sensory neural hearing loss pedigree.: Family and ... ...

Abstract	Objective: Eyes absent 4 (EYA4) is the causative gene of autosomal dominant non-syndromic hereditary hearing loss, DFNA10. We aimed to identify a copy number variation of EYA4 in a non-syndromic sensory neural hearing loss pedigree. Family and clinical evaluation: A Japanese family showing late-onset and progressive hearing loss was evaluated. A pattern of autosomal dominant inheritance of hearing loss was recognized in the pedigree. No cardiac disease was observed in any of the individuals. Methods: Targeted exon sequencing was performed using massively parallel DNA sequencing (MPS) analysis. Scanning of the array comparative genomic hybridization (aCGH) was completed and the copy number variation (CNV) data from the aCGH analysis was confirmed by matching all CNV calls with MPS analysis. Breakpoint detection was performed by whole-genome sequencing and direct sequencing. Sequencing results were examined, and co-segregation analysis of hearing loss was completed. Results: We identified a novel hemizygous indel that showed CNV in the EYA4 gene from the position 133,457,057 to 133,469,892 on chromosome 6 (build GRCh38/hg38) predicted as p.(Val124_Pro323del), and that was segregated with post-lingual and progressive autosomal dominant sensorineural hearing loss by aCGH analysis. Conclusion: Based on the theory of genotype-phenotype correlation with EYA4 mutations in terms of hearing loss and comorbid dilated cardiomyopathy, the region of amino acids 124 to 343 is hypothesized not to be the pathogenic region causing dilated cardiomyopathy. Additionally, the theory of genotype-phenotype correlation about the prevalence of dilated cardiomyopathy is thought to be rejected because of no correlation of deleted amino acid region with the prevalence of dilated cardiomyopathy. These results will help expand the research on both the coordination of cochlear transcriptional regulation and normal cardiac gene regulation via EYA4 transcripts and provide information on the genotype-phenotype correlations of DFNA10 hearing loss.
MeSH term(s)	Comparative Genomic Hybridization ; DNA Copy Number Variations ; Hearing Loss ; Hearing Loss, Sensorineural/genetics ; Humans ; Mutation ; Pedigree ; Trans-Activators
Chemical Substances	EYA4 protein, human ; Trans-Activators
Language	English
Publishing date	2021-04-05
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2036790-9
ISSN	1537-4505 ; 1531-7129
ISSN (online)	1537-4505
ISSN	1531-7129
DOI	10.1097/MAO.0000000000003169
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1554: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Time-course microarrays reveal early activation of the immune transcriptome in a choline-deficient mouse model of liver injury.

Mitsumoto, Koji / Watanabe, Rina / Nakao, Katsuki / Yonenaka, Hisaki / Hashimoto, Takao / Kato, Norihisa / Kumrungsee, Thanutchaporn / Yanaka, Noriyuki

Life sciences

2017 Volume 184, Page(s) 103–111

Abstract: Aims: Choline-deficient diet is extensively used as a model of nonalcoholic fatty liver disease (NAFLD). In this study, we explored genes in the liver for which the expression changed in response to the choline-deficient (CD) diet.: Main methods: ... ...

Abstract	Aims: Choline-deficient diet is extensively used as a model of nonalcoholic fatty liver disease (NAFLD). In this study, we explored genes in the liver for which the expression changed in response to the choline-deficient (CD) diet. Main methods: Male CD-1 mice were divided into two groups and fed a CD diet with or without 0.2% choline bitartrate for one or three weeks. Hepatic levels of choline metabolites were analyzed by using liquid chromatography mass spectrometry and hepatic gene expression profiles were examined by DNA microarray analysis. Key findings: The CD diet lowered liver choline metabolites after one week and exacerbated fatty liver between one and three weeks. We identified >300 genes whose expression was significantly altered in the livers of mice after consumption of this CD diet for one week and showed that liver gene expression profiles could be classified into six distinct groups. This study showed that STAT1 and interferon-regulated genes was up-regulated after the CD diet consumption and that the Stat1 mRNA level was negatively correlated with liver phosphatidylcholine level. Stat1 mRNA expression was actually up-regulated in isolated hepatocytes from the mouse liver with the CD diet. Significance: This study provides insight into the genomic effects of the CD diet through the Stat1 expression, which might be involved in NAFLD development.
MeSH term(s)	Animals ; Choline/metabolism ; Choline Deficiency/complications ; Chromatography, Liquid ; Disease Models, Animal ; Gene Expression Profiling ; Hepatocytes/metabolism ; Liver/metabolism ; Liver/physiopathology ; Male ; Mass Spectrometry ; Mice ; Mice, Inbred ICR ; Non-alcoholic Fatty Liver Disease/genetics ; Non-alcoholic Fatty Liver Disease/physiopathology ; Oligonucleotide Array Sequence Analysis ; Phosphatidylcholines/metabolism ; RNA, Messenger ; STAT1 Transcription Factor/genetics ; Time Factors ; Transcriptome
Chemical Substances	Phosphatidylcholines ; RNA, Messenger ; STAT1 Transcription Factor ; STAT1 protein, human ; Choline (N91BDP6H0X)
Language	English
Publishing date	2017-09-01
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	3378-9
ISSN	1879-0631 ; 0024-3205
ISSN (online)	1879-0631
ISSN	0024-3205
DOI	10.1016/j.lfs.2017.07.009
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Uc I Zs.368: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito