Article ; Online: Cryptanshinone extract of Salvia miltiorrhiza stimulates pediatric acute myeloid leukemia stem cell apoptosis and the anti-inflammatory mechanism via accelerating microRNA-211-5p to supress Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway activation.
Journal of physiology and pharmacology : an official journal of the Polish Physiological Society
2024 Volume 74, Issue 6
Abstract: This study was designed to explore cryptanshinone (CPT) extract of Salvia miltiorrhiza stimulating pediatric acute myeloid leukemia (AML) stem cell (LSC) apoptosis and anti-inflammatory mechanism via accelerating microRNA (miR)-211-5p to restrain Janus ... ...
| Abstract | This study was designed to explore cryptanshinone (CPT) extract of Salvia miltiorrhiza stimulating pediatric acute myeloid leukemia (AML) stem cell (LSC) apoptosis and anti-inflammatory mechanism via accelerating microRNA (miR)-211-5p to restrain Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathway activation. Obtaining blood samples from pediatric acute myeloid leukemia patients and healthy volunteers and detecting miR-211-5p and JAK2 were performed. Purchase of the human AML cell line KG1a was conducted, and sorting of KG1a cells was to gain LSC. Test of miR-211-5p and JAK2, the phosphorylation of JAK2/STAT3 was implemented. Pretreatment of LSCs was with CPT. Variation of miR-211-5p and JAK2 in LSCs was via plasmid transfection to explore their actions in cell advancement with apoptosis and inflammation. Identification of the targeting of miR-211-5p with JAK2 was implemented. In results: MiR-211-5p was declined in endometrial cancer, while JAK2 was elevated; CPT was available to boost LSC apoptosis and restrain the inflammation; elevated miR-211-5p or repressive JAK2 was available to strengthen the acceleration of CPT on LSCs apoptosis and the repression of inflammation; MiR-211-5p targeted JAK2; augmented JAK2 was available to turn around the action of elevated miR-211-5p. We conclude that CPT extract of Salvia miltiorrhiza stimulated pediatric LSC apoptosis and restrained the inflammation via accelerating microRNA (miR)-211-5p to suppress JAK2/STAT3 pathway activation. |
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| MeSH term(s) | Child ; Humans ; Anti-Inflammatory Agents/pharmacology ; Apoptosis ; Cell Proliferation ; Inflammation ; Janus Kinase 2/metabolism ; Leukemia, Myeloid, Acute/drug therapy ; Leukemia, Myeloid, Acute/genetics ; Leukemia, Myeloid, Acute/metabolism ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Salvia miltiorrhiza/chemistry ; Signal Transduction ; STAT3 Transcription Factor/metabolism ; Stem Cells ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use |
| Chemical Substances | Anti-Inflammatory Agents ; Janus Kinase 2 (EC 2.7.10.2) ; MicroRNAs ; MIRN211 microRNA, human ; STAT3 Transcription Factor ; Plant Extracts |
| Language | English |
| Publishing date | 2024-02-07 |
| Publishing country | Poland |
| Document type | Journal Article |
| ZDB-ID | 1125221-2 |
| ISSN | 1899-1505 ; 0867-5910 ; 0044-6033 |
| ISSN (online) | 1899-1505 |
| ISSN | 0867-5910 ; 0044-6033 |
| DOI | 10.26402/jpp.2023.6.09 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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