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Conference proceedings ; Online: Project implementation progress

Wei, Sha

Piloting and scaling of low emission development options in large scale dairy farms in China

2020

Abstract: This presentation was given by Sha Wei at the kick-off meeting on "Piloting and scaling of low emission development options in large scale dairy farms in China" on September 28, 2020. ...

Abstract	This presentation was given by Sha Wei at the kick-off meeting on "Piloting and scaling of low emission development options in large scale dairy farms in China" on September 28, 2020.
Keywords	climate change ; agriculture ; food security ; dairy farms
Language	Chinese
Publishing date	2020-12-18T20:36:58Z
Publisher	CGIAR Research Program on Climate Change, Agriculture and Food Security
Publishing country	fr
Document type	Conference proceedings ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Addition of the T5 exonuclease increases the prime editing efficiency in plants

Liang, Zhen / Wu, Yuqing / Guo, Yingjie / Wei, Sha

Journal of Genetics and Genomics. 2023 Mar. 21,

2023

Abstract: Prime editing (PE) is a versatile genome editing tool without the need for double-stranded DNA breaks or donor DNA templates, but is limited by low editing efficiency. We previously fused the M-MLV reverse transcriptase to the Cas9 nickase, generating ... ...

Abstract	Prime editing (PE) is a versatile genome editing tool without the need for double-stranded DNA breaks or donor DNA templates, but is limited by low editing efficiency. We previously fused the M-MLV reverse transcriptase to the Cas9 nickase, generating the PE2 (v1) system, but the editing efficiency of this system is still low. Here we develop different versions of PE2 by adding the 5'-to-3' exonuclease at different positions of the nCas9-M-MLV RT fusion protein. PE2 (v2), in which the T5 exonuclease fused to the N-terminus of the nCas9-MMLV fusion protein enhances prime editing efficiency of base substitutions, deletions, and insertions at several genomic sites by 1.7- to 2.9-fold in plant cells compared to PE2 (v1). The improved editing efficiency of PE2 (v2) is further confirmed by generating increased heritable prime edits in stable transgenic plants compared to the previously established PE-P1, PE-P2, and PPE systems. Using PE2 (v2), we generate herbicide-resistant rice by simultaneously introducing mutations causing amino acid substitutions at two target sites. The PE efficiency is further improved by combining PE2 (v2) and dual-pegRNAs. Taken together, the increased genome editing efficiency of PE2 (v2) developed in this study may enhance the applications of PE in plants.
Keywords	DNA ; RNA-directed DNA polymerase ; amino acids ; genetically modified organisms ; genome ; genomics ; herbicide resistance ; rice ; Prime editing ; CRISPR ; T5 exonuclease ; Genome editing
Language	English
Dates of publication	2023-0321
Publishing place	Elsevier Ltd
Document type	Article ; Online
Note	Pre-press version ; Use and reproduction
ZDB-ID	2374568-X
ISSN	1873-5533 ; 1673-8527
ISSN (online)	1873-5533
ISSN	1673-8527
DOI	10.1016/j.jgg.2023.03.008
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Genome‑wide identification of MPK and MKK gene families and their responses to phytohormone treatment and abiotic stress in foxtail millet

Liang, Zhen / Wei, Sha / Guo, Yingjie / Wu, Yuqing

Plant Growth Regul. 2023 Jan., v. 99, no. 1 p.85-99

2023

Abstract: The mitogen-activated protein kinase (MAPK) cascade is one of the most important pathways in eukaryotic signaling networks, and it plays a crucial role in plant growth and development, hormonal responses, and responses to various biotic and abiotic ... ...

Abstract	The mitogen-activated protein kinase (MAPK) cascade is one of the most important pathways in eukaryotic signaling networks, and it plays a crucial role in plant growth and development, hormonal responses, and responses to various biotic and abiotic stress. Foxtail millet (Setaria italica) is a minor cereal with excellent nutritional value and fine adaptability to abiotic stress associated with climate change, and it has also emerged as a C₄ model plant. MAPK cascade genes from several model plant species have been analyzed, but not from foxtail millet. Here, 16 SiMPKs and 11 SiMKKs were systematically identified and analyzed in foxtail millet. Phylogenetic relationships, conserved protein motifs, and gene structure indicated clearly that both MPKs and MKKs were divided into four subgroups. RNA-seq data analysis showed that expression profiles of some SiMPK and SiMKK genes varied in different tissues or developmental stages. Furthermore, the expression levels of SiMPK and SiMKK genes under abiotic stresses as well as exogenously applied phytohormone were also investigated. The identified abiotic stress and phytohormone responsive genes suggested that the SiMPKs, SiMKKs, and MKK-MPK interactomes play key roles in abiotic stress and hormone signaling pathways and networks. Our study provides detailed information of MKK and MPK genes in foxtail millet and lays the foundation to explore their functional characterization for stress-tolerance.
Keywords	Setaria italica ; abiotic stress ; climate change ; genes ; growth and development ; mitogen-activated protein kinase ; nutritive value ; phylogeny ; plant growth ; plant hormones ; sequence analysis
Language	English
Dates of publication	2023-01
Size	p. 85-99.
Publishing place	Springer Netherlands
Document type	Article ; Online
ZDB-ID	843025-1
ISSN	1573-5087 ; 0167-6903
ISSN (online)	1573-5087
ISSN	0167-6903
DOI	10.1007/s10725-022-00877-y
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Stray Light Analysis and Suppression of Taiji Telescope for Space Gravitational Wave Detection Based on Phase Noise Requirement

Benliang Sang / Xiaoqin Deng / Wei Tao / Yuqing Diao / Wei Sha

Applied Sciences, Vol 13, Iss 2923, p

2023 Volume 2923

Abstract: The telescope is the core device for space gravitational wave detection. It is responsible for receiving signal light and emitting outgoing light simultaneously. When the high-energy outgoing laser passes through the telescope, it would produce stray ... ...

Abstract	The telescope is the core device for space gravitational wave detection. It is responsible for receiving signal light and emitting outgoing light simultaneously. When the high-energy outgoing laser passes through the telescope, it would produce stray light, which would interfere with the phase measurement and generate phase noise. Therefore, it is necessary to analyze and suppress the stray light of the telescope. In this paper, the requirement of point source transmittance (PST) of the Taiji telescope is obtained through theoretical derivation, which is less than <math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mn>4</mn><mo>×</mo><msup><mrow><mn>10</mn></mrow><mrow><mo>−</mo><mn>10</mn></mrow></msup></mrow></semantics></math> . Through a complete analysis of stray light, we determined that the M4 was the largest source of stray light under the condition that the roughness of each optical surface is equal, which accounted for 67.22%. We also determined the most lenient requirements for surface roughness <math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mi>σ</mi><mi>λ</mi></msub></mrow></semantics></math> , and found that when <math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mi>σ</mi><mi>λ</mi></msub></mrow></semantics></math> of the M1, M2, M3, and M4 were 20 Å, 4 Å, 1 Å, and 1 Å, respectively, the PST was <math xmlns="http://www.w3.org/1998/Math/MathML" ...<br />
Keywords	stray light analysis ; Taiji telescope ; space gravitational wave ; space gravitational wave detection ; surface roughness ; particulate contamination ; Technology ; T ; Engineering (General). Civil engineering (General) ; TA1-2040 ; Biology (General) ; QH301-705.5 ; Physics ; QC1-999 ; Chemistry ; QD1-999
Subject code	535
Language	English
Publishing date	2023-02-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Comparative Proteomic Analysis of Exosomes Derived from Patients Infected with Non-Tuberculous Mycobacterium and Mycobacterium tuberculosis

Li Wang / Xubin Zheng / Jun Ma / Jin Gu / Wei Sha

Microorganisms, Vol 11, Iss 2334, p

2023 Volume 2334

Abstract: The non-tuberculous mycobacterium (NTM) is a very troublesome opportunistic pathogen, placing a heavy burden on public health. The pathogenesis of NTM pulmonary infection is not well-revealed yet, and its diagnosis is always challenging. This study aimed ...

Abstract	The non-tuberculous mycobacterium (NTM) is a very troublesome opportunistic pathogen, placing a heavy burden on public health. The pathogenesis of NTM pulmonary infection is not well-revealed yet, and its diagnosis is always challenging. This study aimed to use a comprehensive proteomics analysis of plasma exosomes to distinguish patients with rapidly growing NTM M. abscessus (MAB), slowly growing NTM M. intracellulare (MAC), and Mycobacterium tuberculosis (MTB). The identified protein components were quantified with label-free proteomics and determined with a bioinformatics analysis. The complement and coagulation were significantly enriched in patients with Mycobacterium infection, and a total of 24 proteins were observed with up-regulation, which included C1R, C1S, C2, MASP2, C4B, C8B, C9, etc. Of them, 18 proteins were significantly up-regulated in patients with MAB, while 6 and 10 were up-regulated in patients with MAC or MTB, respectively. Moreover, MAB infection was also related to the HIF-1 signaling pathway and phagosome processes, and MTB infection was associated with the p53 signaling pathway. This study provided a comprehensive description of the exosome proteome in the plasma of patients infected with MAB, MAC, and MTB and revealed potential diagnostic and differential diagnostic markers.
Keywords	proteome ; exosome ; non-tuberculous mycobacterium ; Mycobacterium tuberculosis complement ; Biology (General) ; QH301-705.5
Subject code	572
Language	English
Publishing date	2023-09-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Cross-sectional survey of smartphone addiction and its relationship with personality traits among medical students.

Huang, Xing / Yang, Haijun / Wei, Sha / Wang, Yan

Australasian psychiatry : bulletin of Royal Australian and New Zealand College of Psychiatrists

2023 Volume 31, Issue 4, Page(s) 535–539

Abstract: Objective: To understand the current smart phone addiction tendency (SPAT) in medical university students and relationship with personality traits.: Methods: A cross-sectional study from September 2019 to December 2019 selected medical students from ... ...

Abstract	Objective: To understand the current smart phone addiction tendency (SPAT) in medical university students and relationship with personality traits. Methods: A cross-sectional study from September 2019 to December 2019 selected medical students from Hubei University of China by cluster sampling. Questionnaires were administered to measure smart phone addiction tendency and personality traits. Results: The prevalence of SPAT among 972 medical students was 24.3%. The prevalence was higher in students from one-child family than those with siblings (28.2% vs. 21.7%), and higher among students from urban families than those from rural ones (29.2% vs. 19.7%).Multivariate logistic regression analysis showed that extraversion and neuroticism were correlated with SPAT, with odds ratio and 95% confidence interval being equal to 1.070 (1.011-1.133) and 0.838 (0.795-0.844), respectively. Conclusions: The prevalence of SPAT was high in medical college students. Extraversion and neuroticism were risk and protective factors for SPAT. Long duration phone use and a low number of physical workouts predicted high SPAT.
MeSH term(s)	Humans ; Students, Medical ; Cross-Sectional Studies ; Personality ; Internet Addiction Disorder ; Neuroticism
Language	English
Publishing date	2023-04-17
Publishing country	England
Document type	Journal Article
ZDB-ID	2213198-X
ISSN	1440-1665 ; 1039-8562
ISSN (online)	1440-1665
ISSN	1039-8562
DOI	10.1177/10398562231167689
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Article: Addition of the T5 exonuclease increases the prime editing efficiency in plants.

Liang, Zhen / Wu, Yuqing / Guo, Yingjie / Wei, Sha

Journal of genetics and genomics = Yi chuan xue bao

2023 Volume 50, Issue 8, Page(s) 582–588

Abstract	Prime editing (PE) is a versatile genome editing tool without the need for double-stranded DNA breaks or donor DNA templates, but is limited by low editing efficiency. We previously fused the M-MLV reverse transcriptase to the Cas9 nickase, generating the PE2 (v1) system, but the editing efficiency of this system is still low. Here we develop different versions of PE2 by adding the 5'-to-3' exonuclease at different positions of the nCas9-M-MLV RT fusion protein. PE2 (v2), in which the T5 exonuclease fused to the N-terminus of the nCas9-MMLV fusion protein enhances prime editing efficiency of base substitutions, deletions, and insertions at several genomic sites by 1.7- to 2.9-fold in plant cells compared to PE2 (v1). The improved editing efficiency of PE2 (v2) is further confirmed by generating increased heritable prime edits in stable transgenic plants compared to the previously established PE-P1, PE-P2, and PPE systems. Using PE2 (v2), we generate herbicide-resistant rice by simultaneously introducing mutations causing amino acid substitutions at two target sites. The PE efficiency is further improved by combining PE2 (v2) and dual-pegRNAs. Taken together, the increased genome editing efficiency of PE2 (v2) developed in this study may enhance the applications of PE in plants.
MeSH term(s)	CRISPR-Cas Systems/genetics ; Gene Editing ; Genome ; Mutation ; Plants, Genetically Modified/genetics
Language	English
Publishing date	2023-03-21
Publishing country	China
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2374568-X
ISSN	1873-5533 ; 1673-8527
ISSN (online)	1873-5533
ISSN	1673-8527
DOI	10.1016/j.jgg.2023.03.008
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Article ; Online: THE NOMOLOGICAL NETWORK VALIDITY OF PERCEIVED FAIRNESS IN BUSINESS-TO-CONSUMER ECOMMERCE

Wei Sha

Issues in Information Systems, Vol 15, Iss 2, Pp 328-

2014 Volume 334

Keywords	Information technology ; T58.5-58.64 ; Electronic computers. Computer science ; QA75.5-76.95
Language	English
Publishing date	2014-01-01T00:00:00Z
Publisher	IACIS
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: The innovative checkpoint inhibitors of lung adenocarcinoma, cg09897064 methylation and ZBP1 expression reduction, have implications for macrophage polarization and tumor growth in lung cancer.

Wang, Ailing / Zheng, Wei-Sha / Luo, Zhen / Bai, Lian / Zhang, Shi

Journal of translational medicine

2024 Volume 22, Issue 1, Page(s) 173

Abstract: Lung cancer, a prevalent and aggressive disease, is characterized by recurrence and drug resistance. It is essential to comprehend the fundamental processes and discover novel therapeutic objectives for augmenting treatment results. Based on our research ...

Abstract	Lung cancer, a prevalent and aggressive disease, is characterized by recurrence and drug resistance. It is essential to comprehend the fundamental processes and discover novel therapeutic objectives for augmenting treatment results. Based on our research findings, we have identified a correlation between methylation of cg09897064 and decreased expression of ZBP1, indicating a link to unfavorable prognosis in patients with lung cancer. Furthermore, these factors play a role in macrophage polarization, with ZBP1 upregulated in M1 macrophages compared to both M0 and M2 polarized macrophages. We observed cg09897064 methylation in M2 polarization, but not in M0 and M1 polarized macrophages. ATACseq analysis revealed closed chromatin accessibility of ZBP1 in M0 polarized macrophages, while open accessibility was observed in both M1 and M2 polarized macrophages. Our findings suggest that ZBP1 is downregulated in M0 polarized macrophages due to closed chromatin accessibility and downregulated in M2 polarized macrophages due to cg09897064 methylation. Further investigations manipulating cg09897064 methylation and ZBP1 expression through overexpression plasmids and shRNAs provided evidence for their role in modulating macrophage polarization and tumor growth. ZBP1 inhibits M2 polarization and suppresses tumor growth, while cg09897064 methylation promotes M2 polarization and macrophage-induced tumor growth. In mechanism investigations, we found that cg09897064 methylation impairs CEBPA binding to the ZBP1 promoter, leading to decreased ZBP1 expression. Clinical experiments were conducted to validate the correlation between methylation at cg09897064, ZBP1 expression, and macrophage M2 polarization. Targeting these factors may hold promise as a strategy for developing innovative checkpoint inhibitors in lung cancer treatment.
MeSH term(s)	Humans ; Adenocarcinoma of Lung/genetics ; Adenocarcinoma of Lung/metabolism ; Chromatin/metabolism ; Lung Neoplasms/genetics ; Lung Neoplasms/metabolism ; Macrophages/metabolism ; Methylation ; RNA-Binding Proteins/genetics
Chemical Substances	Chromatin ; ZBP1 protein, human ; RNA-Binding Proteins
Language	English
Publishing date	2024-02-18
Publishing country	England
Document type	Journal Article
ZDB-ID	2118570-0
ISSN	1479-5876 ; 1479-5876
ISSN (online)	1479-5876
ISSN	1479-5876
DOI	10.1186/s12967-024-04995-1
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Article ; Online: Pyrenecarboxaldehyde@Graphene Oxide Acted as a Highly Efficient ECL Emitter and Target-Triggered the Recyclable Cascade System as an Amplifier for Ultrasensitive APE1 Activity Detection.

Chai, Duo-Duo / Zhuo, Ying / Zhao, Mei-Ling / Li, Hong-Ling / Yuan, Ruo / Wei, Sha-Ping

ACS sensors

2024 Volume 9, Issue 2, Page(s) 955–961

Abstract: Herein, pyrenecarboxaldehyde@graphene oxide (Pyc@GO) sheets with highly efficient electrochemiluminescence (ECL) as emitters were prepared by a noncovalent combination to develop a neoteric ECL biosensing platform for the ultrasensitive assessment of ... ...

Abstract	Herein, pyrenecarboxaldehyde@graphene oxide (Pyc@GO) sheets with highly efficient electrochemiluminescence (ECL) as emitters were prepared by a noncovalent combination to develop a neoteric ECL biosensing platform for the ultrasensitive assessment of human apurinic/apyrimidinic endonuclease1 (APE1) activity. Impressively, the pyrenecarboxaldehyde (Pyc) molecules were able to form stable polar functional groups on the surface of GO sheets through the noncovalent π-π stacking mechanism to prevent intermolecular restacking and simultaneously generate Pyc@GO sheets. Compared with the tightly packed PAH nanocrystals, the Pyc@GO sheets significantly reduced internal filtering effects and diminished nonactivated emitters to enhance ECL intensity and achieve strong ECL emission. Furthermore, the APE1-activated initiators could trigger the recyclable cascade amplified system based on the synergistic cross-activation between catalytic hairpin assembly (CHA) and DNAzyme, which improved the signal amplification and transduction ability. Consequently, the developed ECL platform for the detection of APE1 activity displayed exceptional sensitivity with a low detection limit of 4.6 × 10
MeSH term(s)	Humans ; Catalysis ; DNA, Catalytic ; Graphite ; Nanoparticles
Chemical Substances	graphene oxide ; DNA, Catalytic ; Graphite (7782-42-5)
Language	English
Publishing date	2024-01-22
Publishing country	United States
Document type	Journal Article
ISSN	2379-3694
ISSN (online)	2379-3694
DOI	10.1021/acssensors.3c02425
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