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  1. Article ; Online: Generation of a SIAISi004-A hiPSC line from PBMCs of a 74 year-old Alzheimer’s disease woman by non-integrating sendai virus mediated reprogramming

    Wenxin Zhang / Weihao Di / Jian Zhao / Bei Zhang / Ying Wang

    Stem Cell Research, Vol 55, Iss , Pp 102501- (2021)

    2021  

    Abstract: A 74-year-old Alzheimer’s Disease (AD) woman patient donated her peripheral blood mononuclear cells (PBMC). Non-integrating episomal vector system were used to reprogram their PBMCs with human OKSM (OCT3/4, KLF4, SOX2, and c-MYC) transcription factors. ... ...

    Abstract A 74-year-old Alzheimer’s Disease (AD) woman patient donated her peripheral blood mononuclear cells (PBMC). Non-integrating episomal vector system were used to reprogram their PBMCs with human OKSM (OCT3/4, KLF4, SOX2, and c-MYC) transcription factors. Immunocytochemistry and flow cytometry for pluripotency makers confirmed the pluripotency of transgene-free iPSCs. Pluripotency was confirmed by the ability of iPSCs to spontaneously differentiate three germ layers in vitro as well. The newly generated human iPSC from PBMCs of a 74 year-old Alzheimer’s disease woman can be used for studying further disease mechanisms of Alzheimer’s Disease (AD).
    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2021-08-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Establishment of SIAISi018-A, an induced pluripotent stem cell (iPSC) line from a healthy 45-year-old Chinese Han

    Yi Zhang / Jiajun Shou / Wentao Gu / Weihao Di / Jian Zhao / Ying Wang / Shixin Gu

    Stem Cell Research, Vol 60, Iss , Pp 102659- (2022)

    2022  

    Abstract: A 45-year-old Han Male from China contributed peripheral blood mononuclear cells induced by reprogramming human OKSM transcription factors (OCT3/4, KLF4 SOX2 and C-MYC) with a non-integrated additional vector system. Immunological markers confirmed the ... ...

    Abstract A 45-year-old Han Male from China contributed peripheral blood mononuclear cells induced by reprogramming human OKSM transcription factors (OCT3/4, KLF4 SOX2 and C-MYC) with a non-integrated additional vector system. Immunological markers confirmed the pluripotent nature of IPSC. Spontaneous tridermal differentiation confirmed the differentiation ability of IPSC with normal karyotype.
    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2022-04-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Establishment of SIAISi017-A, an induced pluripotent stem cell(iPSC)line from a healthy 25-year-old Chinese Hui

    Yi Zhang / Jiajun Shou / Wentao Gu / Weihao Di / Jian Zhao / Ying Wang / Shixin Gu

    Stem Cell Research, Vol 60, Iss , Pp 102645- (2022)

    2022  

    Abstract: With the development of cytology, the establishment of cell models in vitro has become a powerful means to study the mechanism and treatment of diseases. Here we successfully generated the IPSC-derived modeling system of a 25-year-old healthy male. His ... ...

    Abstract With the development of cytology, the establishment of cell models in vitro has become a powerful means to study the mechanism and treatment of diseases. Here we successfully generated the IPSC-derived modeling system of a 25-year-old healthy male. His peripheral blood mononuclear cells (PBMC) were reprogrammed using human OKSM (SOX2, OCT3/4, KLF4, and C-MYC) transcription factors using a non-integrated additional vector system. Immunocytochemistry demonstrated that IPSCS expressed all the markers of pluripotency and demonstrated their ability to differentiate spontaneously from three hypoderms in vitro. Karyotype is normal.
    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2022-04-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Generation of a human induced pluripotent stem cell line (SIAISi010-A) from a 31-year-old healthy donor with Chinese Han genetic background

    Qiuting Dai / Weihao Di / Yi Zhang / Shixin Gu / Jian Zhao / Bei Zhang / Ying Wang

    Stem Cell Research, Vol 53, Iss , Pp 102314- (2021)

    2021  

    Abstract: A healthy 31-year-old Chinese Han female donated peripheral blood mononuclear cells (PBMC). Her PBMCs were reprogrammed with human OKSM (OCT3/4, KLF4 SOX2, and c-MYC) transcription factors by the non-integrating episomal vector system. ... ...

    Abstract A healthy 31-year-old Chinese Han female donated peripheral blood mononuclear cells (PBMC). Her PBMCs were reprogrammed with human OKSM (OCT3/4, KLF4 SOX2, and c-MYC) transcription factors by the non-integrating episomal vector system. Immunocytochemistry for pluripotency markers confirmed the pluripotency of transgene-free iPSCs. Their ability to differentiate spontaneously three germ layers in vitro is also confirmed. The iPSC line displayed a normal karyotype. This model can be used as a control in pathological mechanism studies.
    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2021-05-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    Inter-library loan at ZB MED

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  5. Article ; Online: Generation of an induced pluripotent stem cell line (SIAISi009-A) from a 60-year-old Chinese Han female with mild cognitive impairment (MCI) having two copies of APOE4/4 allele

    Jinghui Guo / Weihao Di / Wenxin Zhang / Qiuting Dai / Jian Zhao / Yulei Deng / Ying Wang

    Stem Cell Research, Vol 51, Iss , Pp 102198- (2021)

    2021  

    Abstract: Induced pluripotent stem cells play vitally essential roles in regenerative medicines for disease modeling and drug screening. Here, we successfully generated an iPSC line from PBMC of a 60-year-old female with mild cognitive impairment in an APOE 4/4 ... ...

    Abstract Induced pluripotent stem cells play vitally essential roles in regenerative medicines for disease modeling and drug screening. Here, we successfully generated an iPSC line from PBMC of a 60-year-old female with mild cognitive impairment in an APOE 4/4 background to better understand studies relating to MCI and other cognitive diseases. In the newly-developed hiPSC line SIAISi009-A, all pluripotent markers were well expressed. Moreover, cells displayed a normal karyotype and have differentiation potential proven by in vitro trilineage differentiation method.
    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2021-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Inter-library loan at ZB MED

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  6. Article ; Online: Generation of an induced pluripotent stem cell line (SIAISi003-A) from a 79-year-old patient with Alzheimer’s disease having APOE3/4 genetic background

    Qiuting Dai / Wenxin Zhang / Jinghui Guo / Weihao Di / Jian Zhao / Bei Zhang / Ying Wang

    Stem Cell Research, Vol 48, Iss , Pp 101949- (2020)

    2020  

    Abstract: The genotype of apolipoprotein E (APOE) is closely associated with susceptibility to Alzheimer's disease. Here, we described the generation and characterization of human induced pluripotent stem cells from peripheral blood mononuclear cells (PBMCs) of a ... ...

    Abstract The genotype of apolipoprotein E (APOE) is closely associated with susceptibility to Alzheimer's disease. Here, we described the generation and characterization of human induced pluripotent stem cells from peripheral blood mononuclear cells (PBMCs) of a 79-year-old female patient with Alzheimer's disease having APOE3/4 genotype. The generated iPSCs expressed pluripotent stem cell markers that were observed using immunocytochemistry. Moreover, they displayed a normal karyotype and had the potential to differentiate spontaneously into three germ layers in vitro. Our model could provide valuable insights into pathological mechanisms, and offer a unique opportunity for developing drugs against specific phenotypes for Alzheimer's disease therapy.
    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2020-10-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    Inter-library loan at ZB MED

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  7. Article ; Online: Generation of a human induced pluripotent stem cell line (SIAISi011-A) from a 61-year-old Chinese Han healthy female donor

    Wenxin Zhang / Yishi Hua / Weihao Di / Jinghui Guo / Qiuting Dai / Jian Zhao / Bei Zhang / Ying Wang

    Stem Cell Research, Vol 51, Iss , Pp 102173- (2021)

    2021  

    Abstract: Peripheral blood mononuclear cells (PBMC) were donated by a healthy 61-year old Chinese Han female. Human OKSM (OCT3/4, KLF4 SOX2, and c-MYC) transcription factors were used to reprogram her PBMCs with the non-integrating episomal vector system. ... ...

    Abstract Peripheral blood mononuclear cells (PBMC) were donated by a healthy 61-year old Chinese Han female. Human OKSM (OCT3/4, KLF4 SOX2, and c-MYC) transcription factors were used to reprogram her PBMCs with the non-integrating episomal vector system. Immunocytochemistry for pluripotency makers confirmed the pluripotency of transgene-free iPSCs and their ability to differentiate spontaneously three germ layers in vitro. The iPSC line displayed a normal karyotype. Our model offers the possibility to be used a control in pathological mechanism studies.
    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2021-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  8. Article ; Online: Generation of three iPSC cell lines (SIAISi006-A, SIAISi007-A and SIAISi008-A) from a 66-year-old Alzheimer’s disease (AD) patient and her two unaffected children from Chinese Han population

    Wenxin Zhang / Qiuting Dai / Yishi Hua / Weihao Di / Jinghui Guo / Jian Zhao / Yulei Deng / Ying Wang

    Stem Cell Research, Vol 51, Iss , Pp 102172- (2021)

    2021  

    Abstract: A 66-year-old Chinese Han Alzheimer's Disease (AD) female patient and her two unaffected children donated their Peripheral blood mononuclear cells (PBMC). Non-integrating episomal vector system were used to reprogram their PBMCs with human OKSM (OCT3/4, ... ...

    Abstract A 66-year-old Chinese Han Alzheimer's Disease (AD) female patient and her two unaffected children donated their Peripheral blood mononuclear cells (PBMC). Non-integrating episomal vector system were used to reprogram their PBMCs with human OKSM (OCT3/4, KLF4, SOX2, and c-MYC) transcription factors. Immunocytochemistry for pluripotency makers confirmed the pluripotency of transgene-free iPSCs. Pluripotency was confirmed by the ability of iPSCs to spontaneously differentiate three germ layers in vitro as well. The iPSC line displayed a normal karyotype. This model provides insight into further pathological studies to research identify early biomarkers, study disease pedigrees, and also for drug testing purposes.
    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2021-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Inter-library loan at ZB MED

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  9. Article ; Online: Generation of a human-derived induced pluripotent stem cell line (SIAISi012-A) from an 82-year-old healthy Chinese Han male

    Jinghui Guo / Fang Fang / Qiuting Dai / Wenxin Zhang / Weihao Di / Jiaan Hu / Jian Zhao / Jiumei Cao / Ying Wang

    Stem Cell Research, Vol 52, Iss , Pp 102186- (2021)

    2021  

    Abstract: As the global median population age increases, neurological diseases associated with aging pose significant challenges to human health. Appropriate modeling systems can be useful tools to better understand the mechanism of age-related neuronal ... ...

    Abstract As the global median population age increases, neurological diseases associated with aging pose significant challenges to human health. Appropriate modeling systems can be useful tools to better understand the mechanism of age-related neuronal degeneration diseases. Here, we successfully generated an iPSC-derived modeling system of an 82-year-old healthy man, this newly established line showed that all pluripotent markers were expressed, and the differentiation potential was confirmed by trilineage differentiation. STR profiling proved the cell line identity, and G-binding showed the normal karyotype.
    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2021-04-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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