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  1. Article ; Online: Impact of Graft-Resident Leucocytes on Treg Mediated Skin Graft Survival.

    Steiner, Romy / Weijler, Anna M / Wekerle, Thomas / Sprent, Jonathan / Pilat, Nina

    Frontiers in immunology

    2021  Volume 12, Page(s) 801595

    Abstract: The importance and exact role of graft-resident leucocytes (also referred to as passenger leucocytes) in transplantation is controversial as these cells have been reported to either initiate or retard graft rejection. T cell activation to allografts is ... ...

    Abstract The importance and exact role of graft-resident leucocytes (also referred to as passenger leucocytes) in transplantation is controversial as these cells have been reported to either initiate or retard graft rejection. T cell activation to allografts is mediated
    MeSH term(s) Allografts/immunology ; Animals ; Graft Rejection/immunology ; Graft Survival/immunology ; Immune Tolerance/immunology ; Leukocytes/immunology ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Skin Transplantation ; T-Lymphocytes, Regulatory/immunology
    Language English
    Publishing date 2021-11-29
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2021.801595
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Oleic acid induces the novel apolipoprotein O and reduces mitochondrial membrane potential in chicken and human hepatoma cells

    Weijler, Anna M / Barbara Schmidinger / Stylianos Kapiotis / Hilde Laggner / Marcela Hermann

    Biochimie. 2018 Apr., v. 147

    2018  

    Abstract: Non-alcoholic fatty liver disease (NAFLD) is marked by hepatic fat accumulation and reflects a spectrum of chronic liver diseases associated with obesity, impaired insulin sensitivity and dyslipidemia.Apolipoprotein O (ApoO) is a new member of the plasma ...

    Abstract Non-alcoholic fatty liver disease (NAFLD) is marked by hepatic fat accumulation and reflects a spectrum of chronic liver diseases associated with obesity, impaired insulin sensitivity and dyslipidemia.Apolipoprotein O (ApoO) is a new member of the plasma apolipoprotein family that may play a role in lipid metabolism and electron transport activity of the mitochondrium. However, its physiological functions have not been elucidated yet. Based on our previous data in a non-mammalian experimental system [1], we hypothesized that hepatic expression of ApoO is tightly linked not only to diet-induced hepatosteatosis, but also to increased lipoprotein-production induced by, e.g., hormones and oxidative stress.To gain insight into a mammalian experimental system, we compared the effects of lipid loading on ApoO regulation in chicken hepatoma LMH cells with those in the human hepatoma cell line HepG2. Incubation of the cells with BSA-complexed oleic acid (OA-Alb) induced triglyceride accumulation, but did not affect cell viability. qPCR using specific primer pairs and Western blot analysis with in-house produced rabbit anti-ApoO antisera demonstrated significant increase in ApoO transcript and protein levels in both cell lines. ROS formation due to OA-Alb treatment was only slightly altered in LMH cells, indicating an intact antioxidant defense system of the cells.Oxidative stress applied by addition of H2O2 revealed induction of ApoO transcript and protein level in the same or even higher extent as monitored in the presence of OA-Alb. Upon treatment with estrogen for 24 h quantitative analysis of ApoO transcript and protein revealed increases of ApoO expression supporting the assumption that estrogen affects lipoprotein metabolism at various points. Furthermore, both cell lines showed a significant decrease of the mitochondrial membrane potential upon incubation with OA-Alb. Therefore, we assume that our findings support a role of ApoO as an effector of compromised mitochondrial function that likely accompanies the onset of non-alcoholic fatty liver disease.
    Keywords Western blotting ; antioxidant activity ; antiserum ; cell viability ; chickens ; electron transfer ; estrogens ; fatty liver ; hepatoma ; human cell lines ; humans ; hybridization probes ; hydrogen peroxide ; insulin resistance ; lipid metabolism ; lipoproteins ; membrane potential ; mitochondria ; mitochondrial membrane ; obesity ; oleic acid ; protein metabolism ; quantitative analysis ; quantitative polymerase chain reaction ; rabbits ; triacylglycerols
    Language English
    Dates of publication 2018-04
    Size p. 136-142.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 120345-9
    ISSN 0300-9084
    ISSN 0300-9084
    DOI 10.1016/j.biochi.2018.02.003
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    In stock of ZB MED Bonn / Germany

    Z 72/375: Show issues

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  3. Article ; Online: Oleic acid induces the novel apolipoprotein O and reduces mitochondrial membrane potential in chicken and human hepatoma cells.

    Weijler, Anna M / Schmidinger, Barbara / Kapiotis, Stylianos / Laggner, Hilde / Hermann, Marcela

    Biochimie

    2018  Volume 147, Page(s) 136–142

    Abstract: Non-alcoholic fatty liver disease (NAFLD) is marked by hepatic fat accumulation and reflects a spectrum of chronic liver diseases associated with obesity, impaired insulin sensitivity and dyslipidemia. Apolipoprotein O (ApoO) is a new member of the ... ...

    Abstract Non-alcoholic fatty liver disease (NAFLD) is marked by hepatic fat accumulation and reflects a spectrum of chronic liver diseases associated with obesity, impaired insulin sensitivity and dyslipidemia. Apolipoprotein O (ApoO) is a new member of the plasma apolipoprotein family that may play a role in lipid metabolism and electron transport activity of the mitochondrium. However, its physiological functions have not been elucidated yet. Based on our previous data in a non-mammalian experimental system [1], we hypothesized that hepatic expression of ApoO is tightly linked not only to diet-induced hepatosteatosis, but also to increased lipoprotein-production induced by, e.g., hormones and oxidative stress. To gain insight into a mammalian experimental system, we compared the effects of lipid loading on ApoO regulation in chicken hepatoma LMH cells with those in the human hepatoma cell line HepG2. Incubation of the cells with BSA-complexed oleic acid (OA-Alb) induced triglyceride accumulation, but did not affect cell viability. qPCR using specific primer pairs and Western blot analysis with in-house produced rabbit anti-ApoO antisera demonstrated significant increase in ApoO transcript and protein levels in both cell lines. ROS formation due to OA-Alb treatment was only slightly altered in LMH cells, indicating an intact antioxidant defense system of the cells. Oxidative stress applied by addition of H
    MeSH term(s) Adipose Tissue/drug effects ; Adipose Tissue/pathology ; Animals ; Apolipoproteins/biosynthesis ; Apolipoproteins/genetics ; Apolipoproteins/metabolism ; Carcinoma, Hepatocellular/pathology ; Cell Survival/drug effects ; Chickens ; Hep G2 Cells ; Humans ; Hydrogen Peroxide/pharmacology ; Liver Neoplasms/pathology ; Matrix Metalloproteinases/metabolism ; Membrane Potential, Mitochondrial/drug effects ; Oleic Acid/pharmacology ; Oxidative Stress/drug effects ; RNA, Messenger/genetics ; RNA, Messenger/metabolism
    Chemical Substances APOO protein, human ; Apolipoproteins ; RNA, Messenger ; Oleic Acid (2UMI9U37CP) ; Hydrogen Peroxide (BBX060AN9V) ; Matrix Metalloproteinases (EC 3.4.24.-)
    Language English
    Publishing date 2018-02-10
    Publishing country France
    Document type Journal Article
    ZDB-ID 120345-9
    ISSN 1638-6183 ; 0300-9084
    ISSN (online) 1638-6183
    ISSN 0300-9084
    DOI 10.1016/j.biochi.2018.02.003
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    In stock of ZB MED Cologne/Königswinter

    Uc I Zs.135: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Hepatosteatosis and estrogen increase apolipoprotein O production in the chicken.

    Schmidinger, Barbara / Weijler, Anna M / Schneider, Wolfgang J / Hermann, Marcela

    Biochimie

    2016  Volume 127, Page(s) 37–43

    Abstract: Apolipoprotein O (ApoO) is a recently discovered plasma apolipoprotein that may also play a role in the mitochondrial inner membrane. Possibly due to this complexity, its physiological functions have not been elucidated yet. To gain insight from a non- ... ...

    Abstract Apolipoprotein O (ApoO) is a recently discovered plasma apolipoprotein that may also play a role in the mitochondrial inner membrane. Possibly due to this complexity, its physiological functions have not been elucidated yet. To gain insight from a non-mammalian experimental system, we have investigated the regulation of ApoO levels in an alternative, well-suited model for studies on lipid metabolism, the chicken. qPCR using specific primer pairs and Western blot analysis with our rabbit anti-chicken ApoO antiserum demonstrated ApoO in the liver of chickens fed a control or a fat-enriched diet, as well as in 2 chicken hepatoma cell lines, LMH cells and the estrogen-responsive LMH-2A cells, under conditions of lipid loading by incubation with BSA-complexed oleic acid. Induced triglyceride accumulation in both the liver and the hepatic cells was associated with significantly increased levels of ApoO mRNA and protein. Furthermore, upon treatment for 24 h with estrogen of the estrogen receptor-expressing LMH-2A cells, quantitative analysis of ApoO transcripts and Western blotting revealed increases of ApoO expression. Finally, upon a single administration of estrogen to roosters that leads to hyperlipidemia, higher hepatic levels of both ApoO transcript and protein were observed within 24 h. Based on these data, we propose that hepatic expression of ApoO is tightly linked not only to diet-induced hepatosteatosis, but also to increased lipoprotein-production induced by, e.g., hormones. The findings support a role of ApoO as an effector of compromised mitochondrial function that likely accompanies the onset of non-alcoholic fatty liver disease.
    MeSH term(s) Adipose Tissue/drug effects ; Adipose Tissue/pathology ; Animals ; Apolipoproteins/biosynthesis ; Apolipoproteins/genetics ; Cell Line ; Chickens ; Estrogens/pharmacology ; Female ; Gene Expression Regulation/drug effects ; Liver/drug effects ; Liver/metabolism ; Liver/pathology ; Male ; Non-alcoholic Fatty Liver Disease/genetics ; Non-alcoholic Fatty Liver Disease/metabolism ; Non-alcoholic Fatty Liver Disease/pathology ; RNA, Messenger/genetics ; RNA, Messenger/metabolism
    Chemical Substances Apolipoproteins ; Estrogens ; RNA, Messenger
    Language English
    Publishing date 2016-08
    Publishing country France
    Document type Journal Article
    ZDB-ID 120345-9
    ISSN 1638-6183 ; 0300-9084
    ISSN (online) 1638-6183
    ISSN 0300-9084
    DOI 10.1016/j.biochi.2016.04.017
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Uc I Zs.135: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  5. Article: Hepatosteatosis and estrogen increase apolipoprotein O production in the chicken

    Schmidinger, Barbara / Weijler, Anna M / Schneider, Wolfgang J / Hermann, Marcela

    Biochimie. 2016 Aug., v. 127

    2016  

    Abstract: Apolipoprotein O (ApoO) is a recently discovered plasma apolipoprotein that may also play a role in the mitochondrial inner membrane. Possibly due to this complexity, its physiological functions have not been elucidated yet. To gain insight from a non- ... ...

    Abstract Apolipoprotein O (ApoO) is a recently discovered plasma apolipoprotein that may also play a role in the mitochondrial inner membrane. Possibly due to this complexity, its physiological functions have not been elucidated yet. To gain insight from a non-mammalian experimental system, we have investigated the regulation of ApoO levels in an alternative, well-suited model for studies on lipid metabolism, the chicken. qPCR using specific primer pairs and Western blot analysis with our rabbit anti-chicken ApoO antiserum demonstrated ApoO in the liver of chickens fed a control or a fat-enriched diet, as well as in 2 chicken hepatoma cell lines, LMH cells and the estrogen-responsive LMH-2A cells, under conditions of lipid loading by incubation with BSA-complexed oleic acid. Induced triglyceride accumulation in both the liver and the hepatic cells was associated with significantly increased levels of ApoO mRNA and protein. Furthermore, upon treatment for 24 h with estrogen of the estrogen receptor-expressing LMH-2A cells, quantitative analysis of ApoO transcripts and Western blotting revealed increases of ApoO expression. Finally, upon a single administration of estrogen to roosters that leads to hyperlipidemia, higher hepatic levels of both ApoO transcript and protein were observed within 24 h. Based on these data, we propose that hepatic expression of ApoO is tightly linked not only to diet-induced hepatosteatosis, but also to increased lipoprotein-production induced by, e.g., hormones. The findings support a role of ApoO as an effector of compromised mitochondrial function that likely accompanies the onset of non-alcoholic fatty liver disease.
    Keywords Western blotting ; antiserum ; binding proteins ; diet ; fatty liver ; hepatoma ; hormones ; hyperlipidemia ; lipid metabolism ; liver ; messenger RNA ; mitochondria ; mitochondrial membrane ; models ; oleic acid ; quantitative analysis ; quantitative polymerase chain reaction ; rabbits ; roosters ; triacylglycerols
    Language English
    Dates of publication 2016-08
    Size p. 37-43.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 120345-9
    ISSN 0300-9084
    ISSN 0300-9084
    DOI 10.1016/j.biochi.2016.04.017
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 72/375: Show issues

    Order via subito

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    Inter-library loan at ZB MED

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  6. Article ; Online: Distinct roles for major and minor antigen barriers in chimerism-based tolerance under irradiation-free conditions.

    Mahr, Benedikt / Pilat, Nina / Granofszky, Nicolas / Muckenhuber, Moritz / Unger, Lukas W / Weijler, Anna M / Wiletel, Mario / Steiner, Romy / Dorner, Lisa / Regele, Heinz / Wekerle, Thomas

    American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

    2020  Volume 21, Issue 3, Page(s) 968–977

    Abstract: Eliminating cytoreductive conditioning from chimerism-based tolerance protocols would facilitate clinical translation. Here we investigated the impact of major histocompatibility complex (MHC) and minor histocompatibility antigen (MiHA) barriers on ... ...

    Abstract Eliminating cytoreductive conditioning from chimerism-based tolerance protocols would facilitate clinical translation. Here we investigated the impact of major histocompatibility complex (MHC) and minor histocompatibility antigen (MiHA) barriers on mechanisms of tolerance and rejection in this setting. Transient depletion of natural killer (NK) cells at the time of bone marrow (BM) transplantation (BMT) (20 × 10
    MeSH term(s) Animals ; Bone Marrow Transplantation ; Chimerism ; Immune Tolerance ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Skin Transplantation ; Transplantation Chimera ; Transplantation Tolerance
    Language English
    Publishing date 2020-07-24
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2060594-8
    ISSN 1600-6143 ; 1600-6135
    ISSN (online) 1600-6143
    ISSN 1600-6135
    DOI 10.1111/ajt.16177
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5542: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  7. Article ; Online: Treg-mediated prolonged survival of skin allografts without immunosuppression.

    Pilat, Nina / Wiletel, Mario / Weijler, Anna M / Steiner, Romy / Mahr, Benedikt / Warren, Joanna / Corpuz, Theresa M / Wekerle, Thomas / Webster, Kylie E / Sprent, Jonathan

    Proceedings of the National Academy of Sciences of the United States of America

    2019  Volume 116, Issue 27, Page(s) 13508–13516

    Abstract: Injection of Interleukin-2 (IL-2) complexed with a particular anti-IL-2 monoclonal antibody (mab) JES6-1 has been shown to selectively expand ... ...

    Abstract Injection of Interleukin-2 (IL-2) complexed with a particular anti-IL-2 monoclonal antibody (mab) JES6-1 has been shown to selectively expand CD4
    MeSH term(s) Allografts ; Animals ; Antibodies, Monoclonal/immunology ; Female ; Flow Cytometry ; Graft Rejection/prevention & control ; Graft Survival/immunology ; Immunosuppressive Agents/therapeutic use ; Interleukin-2/immunology ; Interleukin-6/antagonists & inhibitors ; Mice, Inbred BALB C ; Mice, Inbred C3H ; Mice, Inbred C57BL ; Sirolimus/therapeutic use ; Skin Transplantation ; T-Lymphocytes, Regulatory/immunology
    Chemical Substances Antibodies, Monoclonal ; Immunosuppressive Agents ; Interleukin-2 ; Interleukin-6 ; Sirolimus (W36ZG6FT64)
    Language English
    Publishing date 2019-06-13
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.1903165116
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1148: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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