Result 1 - 10 of total 10
Umweltschutz, Biozidverordnung und Chemikaliengesetz
2023
| Keywords | Text ; ddc:630 |
|---|---|
| Language | German |
| Publishing date | 2023-03-20 |
| Publishing country | de |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
International journal of medical microbiology : IJMM
2024 Volume 314, Page(s) 151609
Abstract: Interspecies transmission of influenza A viruses (IAV) from pigs to humans is a concerning event as porcine IAV represent a reservoir of potentially pandemic IAV. We conducted a comprehensive analysis of two porcine A(H1N1)v viruses isolated from human ... ...
| Abstract | Interspecies transmission of influenza A viruses (IAV) from pigs to humans is a concerning event as porcine IAV represent a reservoir of potentially pandemic IAV. We conducted a comprehensive analysis of two porcine A(H1N1)v viruses isolated from human cases by evaluating their genetic, antigenic and virological characteristics. The HA genes of those human isolates belonged to clades 1C.2.1 and 1C.2.2, respectively, of the A(H1N1) Eurasian avian-like swine influenza lineage. Antigenic profiling revealed substantial cross-reactivity between the two zoonotic H1N1 viruses and human A(H1N1)pdm09 virus and some swine viruses, but did not reveal cross-reactivity to H1N2 and earlier human seasonal A(H1N1) viruses. The solid-phase direct receptor binding assay analysis of both A(H1N1)v showed a predominant binding to α2-6-sialylated glycans similar to human-adapted IAV. Investigation of the replicative potential revealed that both A(H1N1)v viruses grow in human bronchial epithelial cells to similar high titers as the human A(H1N1)pdm09 virus. Cytokine induction was studied in human alveolar epithelial cells A549 and showed that both swine viruses isolated from human cases induced higher amounts of type I and type III IFN, as well as IL6 compared to a seasonal A(H1N1) or a A(H1N1)pdm09 virus. In summary, we demonstrate a remarkable adaptation of both zoonotic viruses to propagate in human cells. Our data emphasize the needs for continuous monitoring of people and regions at increased risk of such trans-species transmissions, as well as systematic studies to quantify the frequency of these events and to identify viral molecular determinants enhancing the zoonotic potential of porcine IAV. |
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| MeSH term(s) | Humans ; Animals ; Swine ; Influenza A Virus, H1N1 Subtype/genetics ; Orthomyxoviridae Infections/epidemiology ; Orthomyxoviridae Infections/veterinary ; Influenza, Human/epidemiology ; Germany/epidemiology ; Swine Diseases/epidemiology ; Phylogeny |
| Language | English |
| Publishing date | 2024-01-24 |
| Publishing country | Germany |
| Document type | Journal Article |
| ZDB-ID | 2006518-8 |
| ISSN | 1618-0607 ; 1438-4221 |
| ISSN (online) | 1618-0607 |
| ISSN | 1438-4221 |
| DOI | 10.1016/j.ijmm.2024.151609 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Zs.A 5333: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG) |
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Umweltschutz, Biozidverordnung und Chemikaliengesetz
2020
| Keywords | Text ; ddc:630 |
|---|---|
| Language | German |
| Publishing date | 2020-11-19 |
| Publishing country | de |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz
2021 Volume 65, Issue 1, Page(s) 86–95
Abstract: Disinfection measures have become more important as a result of the COVID-19 pandemic in Germany. The increased need for disinfectants at the beginning of the pandemic required temporary legal regulations in order to provide a sufficient quantity of ... ...
| Title translation | Disinfectants during the COVID-19 pandemic: a challenge. |
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| Abstract | Disinfection measures have become more important as a result of the COVID-19 pandemic in Germany. The increased need for disinfectants at the beginning of the pandemic required temporary legal regulations in order to provide a sufficient quantity of products for the necessary disinfection in the medical sector on the one hand and for the additional demand in the population on the other. For this purpose, the Federal Institute for Drugs and Medical Devices (BfArM) and the Federal Institute for Occupational Safety and Health (BAuA) issued a general ruling, which is explained in more detail in this article. The focus was on measures for hygienic hand disinfection. However, other applications such as surface disinfection in relation to pandemic respiratory diseases are also addressed. The experience gained in ensuring the supply of disinfectants that are effective and safe to use should be used to prepare for further pandemics. |
| MeSH term(s) | COVID-19 ; Disinfectants ; Disinfection ; Germany ; Humans ; Pandemics/prevention & control ; SARS-CoV-2 |
| Chemical Substances | Disinfectants |
| Language | German |
| Publishing date | 2021-12-08 |
| Publishing country | Germany |
| Document type | Journal Article ; Review |
| ZDB-ID | 1461973-8 |
| ISSN | 1437-1588 ; 1436-9990 |
| ISSN (online) | 1437-1588 |
| ISSN | 1436-9990 |
| DOI | 10.1007/s00103-021-03457-z |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Un I Zs.525: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG) |
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Hintergründe der Überarbeitung und die wichtigsten Neuerungen
2021
| Keywords | Text ; ddc:570 ; Empfehlungen des Friedrich-Loeffler-Instituts über Mittel und Verfahren für die Durchführung einer tierseuchenrechtlich vorgeschriebenen Desinfektion -- Desinfektions-Richtlinie |
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| Language | German |
| Publishing date | 2021-01-13 |
| Publishing country | de |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
2020
Abstract: Nach Erkennung und Merzung infizierter Tiere spielt die Reinigung und Desinfektion der betroffenen Tierhaltungen eine zentrale Rolle in der Tierseuchenbekämpfung. Vom Bundesministerium wurde 1993 eine Richtlinie (D-RL) veröffentlicht, nach der die ... ...
| Abstract | Nach Erkennung und Merzung infizierter Tiere spielt die Reinigung und Desinfektion der betroffenen Tierhaltungen eine zentrale Rolle in der Tierseuchenbekämpfung. Vom Bundesministerium wurde 1993 eine Richtlinie (D-RL) veröffentlicht, nach der die zuständige Behörde die in Bekämpfungs-verordnungen vorgeschriebene Desinfektion anweisen kann. Aufgrund der technischen Fortentwicklung der Tierhaltung und gravierender Neuerungen im Biozidrecht wurde das Friedrich-Loeffler-Institut (FLI) gebeten, die Richtlinie zu überarbeiten. |
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| Keywords | Text ; article ; ddc:610 |
| Language | German |
| Publishing date | 2020-10-01 |
| Publishing country | de |
| Document type | Article ; Online |
| Database | BASE - Bielefeld Academic Search Engine (life sciences selection) |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
2015 Volume 89, Issue 14, Page(s) 6982–6993
Abstract: Unlabelled: Influenza A virus (IAV) infection provokes an antiviral response involving the expression of type I and III interferons (IFN) and IFN-stimulated genes (ISGs) in infected cell cultures. However, the spatiotemporal dynamics of the IFN reaction ...
| Abstract | Unlabelled: Influenza A virus (IAV) infection provokes an antiviral response involving the expression of type I and III interferons (IFN) and IFN-stimulated genes (ISGs) in infected cell cultures. However, the spatiotemporal dynamics of the IFN reaction are incompletely understood, as previous studies investigated mainly the population responses of virus-infected cultures, although substantial cell-to-cell variability has been documented. We devised a fluorescence-activated cell sorting-based assay to simultaneously quantify expression of viral antigens and ISGs, such as ISG15, MxA, and IFIT1, in IAV-infected cell cultures at the single-cell level. This approach revealed that seasonal IAV triggers an unexpected asymmetric response, as the major cell populations expressed either viral antigen or ISG, but rarely both. Further investigations identified a role of the viral NS1 protein in blocking ISG expression in infected cells, which surprisingly did not reduce paracrine IFN signaling to noninfected cells. Interestingly, viral ISG control was impaired in cultures infected with avian-origin IAV, including the H7N9 virus from eastern China. This phenotype was traced back to polymorphic NS1 amino acids known to be important for stable binding of the polyadenylation factor CPSF30 and concomitant suppression of host cell gene expression. Most significantly, mutation of two amino acids within the CPSF30 attachment site of NS1 from seasonal IAV diminished the strict control of ISG expression in infected cells and substantially attenuated virus replication. In conclusion, our approach revealed an asymmetric, NS1-dependent ISG induction in cultures infected with seasonal IAV, which appears to be essential for efficient virus propagation. Importance: Interferons are expressed by infected cells in response to IAV infection and play important roles in the antiviral immune response by inducing hundreds of interferon-stimulated genes (ISGs). Unlike many previous studies, we investigated the ISG response at the single-cell level, enabling novel insights into this virus-host interaction. Hence, cell cultures infected with seasonal IAV displayed an asymmetric ISG induction that was confined almost exclusively to noninfected cells. In comparison, ISG expression was observed in larger cell populations infected with avian-origin IAV, suggesting a more resolute antiviral response to these strains. Strict control of ISG expression by seasonal IAV was explained by the binding of the viral NS1 protein to the polyadenylation factor CPSF30, which reduces host cell gene expression. Mutational disruption of CPSF30 binding within NS1 concomitantly attenuated ISG control and replication of seasonal IAV, illustrating the importance of maintaining an asymmetric ISG response for efficient virus propagation. |
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| MeSH term(s) | China ; Cleavage And Polyadenylation Specificity Factor/metabolism ; Flow Cytometry/methods ; Gene Expression Profiling ; Gene Expression Regulation ; Host-Pathogen Interactions ; Humans ; Influenza A virus/immunology ; Interferons/metabolism ; Mutant Proteins/genetics ; Mutant Proteins/metabolism ; Single-Cell Analysis ; Viral Nonstructural Proteins/genetics ; Viral Nonstructural Proteins/metabolism |
| Chemical Substances | CPSF4 protein, human ; Cleavage And Polyadenylation Specificity Factor ; INS1 protein, influenza virus ; Mutant Proteins ; Viral Nonstructural Proteins ; Interferons (9008-11-1) |
| Language | English |
| Publishing date | 2015-04-22 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 80174-4 |
| ISSN | 1098-5514 ; 0022-538X |
| ISSN (online) | 1098-5514 |
| ISSN | 0022-538X |
| DOI | 10.1128/JVI.00857-15 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Zs.A 609: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
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2011
| Author's details | Viola Weinheimer. Gutachter: Richard Lucius ; Thorsten Wolff ; Andreas Herrmann |
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| Keywords | Biowissenschaften, Biologie ; Life Science, Biology |
| Subject code | sg570 |
| Language | German |
| Publisher | Humboldt Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I |
| Publishing place | Berlin |
| Document type | Book ; Online ; Thesis |
| Database | Digital theses on the web |
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.
2006 Volume 56, Issue 3, Page(s) 196–201
Abstract: Recent studies have shown that 'return to home cage' can serve as a reward for maze learning in adult male mice. The present study examined whether the same reward is an effective motivator of learning in young and old mice and included females in the ... ...
| Abstract | Recent studies have shown that 'return to home cage' can serve as a reward for maze learning in adult male mice. The present study examined whether the same reward is an effective motivator of learning in young and old mice and included females in the study design. We tested 25- and 65-d-old HS mice and 85- and 800-d-old B6D2F2 mice in a Lashley III maze. Return to home cage motivated maze acquisition in all groups. Compared with 65-d-old HS mice, 25-d-olds acquired the maze more slowly, took longer to achieve the test criterion, and showed increased latency to reach the goal box. There was no difference between 85- and 800-d-old B6D2F2 mice in rate of acquisition. This reward procedure may reduce the potentially confounding effects of deprivation or aversive stimuli on maze performance and may be suitable as a motivational procedure for a wide range of subject groups. |
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| MeSH term(s) | Aging/genetics ; Animals ; Female ; Genotype ; Homing Behavior/physiology ; Male ; Maze Learning/physiology ; Mental Recall ; Mice ; Mice, Inbred Strains ; Models, Animal ; Motivation ; Orientation ; Reward ; Social Environment |
| Language | English |
| Publishing date | 2006-06 |
| Publishing country | United States |
| Document type | Comparative Study ; Journal Article ; Research Support, N.I.H., Extramural |
| ZDB-ID | 2006425-1 |
| ISSN | 1532-0820 ; 0023-6764 |
| ISSN | 1532-0820 ; 0023-6764 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Zs.B 601: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG) |
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The Journal of infectious diseases
2012 Volume 206, Issue 11, Page(s) 1685–1694
Abstract: Background: Highly pathogenic avian H5N1 influenza viruses preferentially infect alveolar type II pneumocytes in human lung. However, it is unknown whether this cellular tropism contributes to high viral virulence because the primary target cells of ... ...
| Abstract | Background: Highly pathogenic avian H5N1 influenza viruses preferentially infect alveolar type II pneumocytes in human lung. However, it is unknown whether this cellular tropism contributes to high viral virulence because the primary target cells of other influenza viruses have not been systematically studied. Methods: We provide the first comparison of the replication, tropism, and cytokine induction of human, highly pathogenic avian influenza A virus subtype H5N1 and other animal influenza A viruses in primary human lung organ cultures. Results: Subytpe H5N1 and human-adapted subtype H1N1 and H3N2 viruses replicated efficiently in the lung tissue, whereas classic swine and low-pathogenicity avian viruses propagated only poorly. Nevertheless, all viruses examined were detected almost exclusively in type II pneumocytes, with a minor involvement of alveolar macrophages. Infection with avian viruses that have a low and high pathogenicity provoked a pronounced induction of cytokines and chemokines, while human and pandemic H1N1-2009 viruses triggered only weak responses. Conclusions: These findings show that differences in the pathogenic potential of influenza A viruses in the human lung cannot be attributed to a distinct cellular tropism. Rather, high or low viral pathogenicity is associated with a strain-specific capacity to productively replicate in type II pneumocytes and to cope with the induced cytokine response. |
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| MeSH term(s) | Alveolar Epithelial Cells/classification ; Alveolar Epithelial Cells/virology ; Cytokines/genetics ; Cytokines/metabolism ; Gene Expression Regulation/physiology ; Humans ; Influenza A virus/classification ; Influenza A virus/pathogenicity ; Influenza A virus/physiology ; Influenza, Human/virology ; Lung/cytology ; Macrophages, Alveolar/virology ; Tissue Culture Techniques ; Viral Tropism/physiology ; Virulence ; Virus Replication/physiology |
| Chemical Substances | Cytokines |
| Keywords | covid19 |
| Language | English |
| Publishing date | 2012-07-24 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, U.S. Gov't, P.H.S. |
| ZDB-ID | 3019-3 |
| ISSN | 1537-6613 ; 0022-1899 |
| ISSN (online) | 1537-6613 |
| ISSN | 0022-1899 |
| DOI | 10.1093/infdis/jis455 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
| Uh II Zs.50: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG) |
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| Zs.MO 445: Show issues |
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