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  1. Article ; Online: Editorial

    Yanwen Peng / Weiqiang Li / Qunzhou Zhang

    Frontiers in Immunology, Vol

    Immunomodulation of MSCs in tissue repairing and regeneration

    2023  Volume 14

    Keywords MSC ; immunomodulation ; regenerative medicine ; tissue repair ; secretomes ; inflammatory diseases ; Immunologic diseases. Allergy ; RC581-607
    Language English
    Publishing date 2023-02-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Exploring CYGNSS mission for surface heat flux estimates and analysis over tropical oceans

    Xiaohui Li / Jingsong Yang / Yunwei Yan / Weiqiang Li

    Frontiers in Marine Science, Vol

    2022  Volume 9

    Abstract: The Cyclone Global Navigation Satellite System (CYGNSS) mission can measure sea surface wind over tropical oceans with unprecedented temporal resolution and spatial coverage, so as to estimate surface latent and sensible heat fluxes (LHF and SHF). In ... ...

    Abstract The Cyclone Global Navigation Satellite System (CYGNSS) mission can measure sea surface wind over tropical oceans with unprecedented temporal resolution and spatial coverage, so as to estimate surface latent and sensible heat fluxes (LHF and SHF). In this paper, the satellite-derived LHF/SHF estimates from CYGNSS are quantitatively evaluated and analyzed by those from the Global Tropical Moored Buoy. Comparisons of the LHF and SHF estimates demonstrate the good performance and reliability of CYGNSS heat flux products during the period of 2017–2022, including CYGNSS Level 2 Ocean Surface Heat Flux Climate Data Record (CDR) Version 1.0 and Version 1.1. Different latent heat characteristics in the tropical oceans are evaluated separately based on each buoy array, suggesting better agreement in the Atlantic for LHF/SHF products. Based on the Coupled Ocean-Atmosphere Response Experiment 3.5 algorithm, the impact of wind speed on the LHF/SHF estimates is analyzed by using the the Science Data Record V3.1 and NOAA V1.2 science wind products. The results show that the performance of satellite-derived wind speed directly affects the accuracy of LHF products, with an improvement of 17% in root-mean-square error over that of LHF CDR V1.0. Especially, in the Indian Ocean, accuracy can be improved by 26.8%. This paper demonstrates that the heat flux estimates along the orbit of the CYGNSS are an important supplement to in situ observational data and will benefit the study of global climate change.
    Keywords global navigation satellite system reflectometry (GNSS-R) ; cyclone GNSS (CYGNSS) ; surface heat fluxes ; latent heat flux ; sensible heat flux ; tropical ocean ; Science ; Q ; General. Including nature conservation ; geographical distribution ; QH1-199.5
    Subject code 551
    Language English
    Publishing date 2022-11-01T00:00:00Z
    Publisher Frontiers Media S.A.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Sclerotinia sclerotiorum (Lib.) de Bary

    Md. Motaher Hossain / Farjana Sultana / Weiqiang Li / Lam-Son Phan Tran / Mohammad Golam Mostofa

    Cells, Vol 12, Iss 1063, p

    Insights into the Pathogenomic Features of a Global Pathogen

    2023  Volume 1063

    Abstract: Sclerotinia sclerotiorum (Lib.) de Bary is a broad host-range fungus that infects an inclusive array of plant species and afflicts significant yield losses globally. Despite being a notorious pathogen, it has an uncomplicated life cycle consisting of ... ...

    Abstract Sclerotinia sclerotiorum (Lib.) de Bary is a broad host-range fungus that infects an inclusive array of plant species and afflicts significant yield losses globally. Despite being a notorious pathogen, it has an uncomplicated life cycle consisting of either basal infection from myceliogenically germinated sclerotia or aerial infection from ascospores of carpogenically germinated sclerotia. This fungus is unique among necrotrophic pathogens in that it inevitably colonizes aging tissues to initiate an infection, where a saprophytic stage follows the pathogenic phase. The release of cell wall-degrading enzymes, oxalic acid, and effector proteins are considered critical virulence factors necessary for the effective pathogenesis of S. sclerotiorum . Nevertheless, the molecular basis of S. sclerotiorum pathogenesis is still imprecise and remains a topic of continuing research. Previous comprehensive sequencing of the S. sclerotiorum genome has revealed new insights into its genome organization and provided a deeper comprehension of the sophisticated processes involved in its growth, development, and virulence. This review focuses on the genetic and genomic aspects of fungal biology and molecular pathogenicity to summarize current knowledge of the processes utilized by S. sclerotiorum to parasitize its hosts. Understanding the molecular mechanisms regulating the infection process of S. sclerotiorum will contribute to devising strategies for preventing infections caused by this destructive pathogen.
    Keywords cAMP ; cell wall degrading enzymes ; effectors ; life cycle ; molecular diagnosis ; oxalic acid ; Biology (General) ; QH301-705.5
    Subject code 580
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: Generation of a MSX1 knockout human embryonic stem cell line using CRISPR/Cas9 technology

    Weicheng Chiu / Anqi Li / Tao Wang / Weiqiang Li / Xinchun Zhang

    Stem Cell Research, Vol 60, Iss , Pp 102729- (2022)

    2022  

    Abstract: The MSX1 gene encodes a transcriptional repressor and plays important roles in limb-pattern formation, craniofacial development, and odontogenesis during vertebrate embryogenesis. Previous studies demonstrated that human MSX1 mutations are associated ... ...

    Abstract The MSX1 gene encodes a transcriptional repressor and plays important roles in limb-pattern formation, craniofacial development, and odontogenesis during vertebrate embryogenesis. Previous studies demonstrated that human MSX1 mutations are associated with tooth agenesis, orofacial clefting, and nail dysplasia. Here, we generated a MSX1 knockout cell line from human embryonic stem cell (hESC) line (H9) by CRISPR/cas9-mediated gene targeting. This cell line may serve as a valuable in vitro cell model for MSX1 mutation-related diseases and help to gain more insight into the biological function of MSX1.
    Keywords Biology (General) ; QH301-705.5
    Language English
    Publishing date 2022-04-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Exploration of Multi-Mission Spaceborne GNSS-R Raw IF Data Sets

    Weiqiang Li / Estel Cardellach / Serni Ribó / Santi Oliveras / Antonio Rius

    Remote Sensing, Vol 14, Iss 1344, p

    Processing, Data Products and Potential Applications

    2022  Volume 1344

    Abstract: Earth reflected Global Navigation Satellite System (GNSS) signals can be received by dedicated orbital receivers for remote sensing and Earth observation (EO) purposes. Different spaceborne missions have been launched during the past years, most of which ...

    Abstract Earth reflected Global Navigation Satellite System (GNSS) signals can be received by dedicated orbital receivers for remote sensing and Earth observation (EO) purposes. Different spaceborne missions have been launched during the past years, most of which can only provide the delay-Doppler map (DDM) of the power of the reflected GNSS signals as their main data products. In addition to the power DDM products, some of these missions have collected a large amount of raw intermediate frequency (IF) data, which are the bit streams of raw signal samples recorded after the analog-to-digital converters (ADCs) and prior to any onboard digital processing. The unprocessed nature of these raw IF data provides an unique opportunity to explore the potential of GNSS Reflectometry (GNSS-R) technique for advanced geophysical applications and future spaceborne missions. To facilitate such explorations, the raw IF data sets from different missions have been processed by Institute of Space Sciences (ICE-CSIC, IEEC), and the corresponding data products, i.e., the complex waveform of the reflected signal, have been generated and released through our public open-data server. These complex waveform data products provide the measurements from different GNSS constellations (e.g., GPS, Galileo and BeiDou), and include both the amplitude and carrier phase information of the reflected GNSS signal at higher sampling rate (e.g., 1000 Hz). To demonstrate these advanced features of the data products, different applications, e.g., inland water detection and surface altimetry, are introduced in this paper. By making these complex waveform data products publicly available, new EO capability of the GNSS-R technique can be further explored by the community. Such early explorations are also relevant to ESA’s next GNSS-R mission, HydroGNSS, which will provide similar complex observations operationally and continuously in the future.
    Keywords Global Navigation Satellite System Reflectometry (GNSS-R) ; spaceborne mission ; raw intermediate frequency (IF) samples ; carrier phase ; signal coherence ; Science ; Q
    Subject code 550
    Language English
    Publishing date 2022-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Transcriptomic and Metabolomic Analyses of the Effects of Exogenous Trehalose on Heat Tolerance in Wheat

    Yin Luo / Yue Wang / Yanyang Xie / Yamin Gao / Weiqiang Li / Shuping Lang

    International Journal of Molecular Sciences, Vol 23, Iss 5194, p

    2022  Volume 5194

    Abstract: Trehalose can improve the tolerance of plants to various types of environmental stress. Nonetheless, information respecting the molecular networks of wheat seedlings to exogenous trehalose under heat stress is limited. Here, two wheat varieties ... ...

    Abstract Trehalose can improve the tolerance of plants to various types of environmental stress. Nonetheless, information respecting the molecular networks of wheat seedlings to exogenous trehalose under heat stress is limited. Here, two wheat varieties pretreated with exogenous trehalose were selected to explore the molecular mechanism by which trehalose improves the heat tolerance of wheat ( Triticum aestivum L.). The results indicated that exogenous trehalose improved the physiological state of wheat seedlings under heat stress. Through RNA sequencing and metabolomics analysis, the genes and metabolites specifically expressed in trehalose pretreatment were identified. After heat stress, there were 18,352 differentially expressed genes (DEGs) in the control and trehalose-treated (H_vs_TreH) groups of Yangmai 18 and 9045 DEGs in Yannong 19. Functional annotation and enrichment analyses showed that the DEGs in the two wheat varieties were mainly involved in carbohydrate metabolism and biosynthesis of secondary metabolites. Through a liquid chromatography–mass spectrometry platform, 183 differential metabolites in H_vs_TreH groups of Yangmai 18 and 77 differential metabolites in Yannong 19 were identified. Compared with the control group, many protective metabolites, such as amino acids, purines, phenylpropanoids and flavonoids, showed significant differences under heat stress. The results indicated that exogenous trehalose protected the wheat biomembrane system, enhanced carbohydrate metabolism and signal transduction, strengthened the activity of the tricarboxylic acid cycle (TCA cycle), regulated purine metabolism, gene expression and metabolite accumulation in the phenylpropanoid biosynthesis and flavonoid biosynthesis pathways, thus improving the heat tolerance of wheat.
    Keywords trehalose ; wheat ; heat stress ; transcriptome ; metabolome ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 580
    Language English
    Publishing date 2022-05-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Signal-to-Noise Ratio Analyses of Spaceborne GNSS-Reflectometry from Galileo and BeiDou Satellites

    Yang Nan / Shirong Ye / Jingnan Liu / Bofeng Guo / Shuangcheng Zhang / Weiqiang Li

    Remote Sensing, Vol 14, Iss 35, p

    2022  Volume 35

    Abstract: In recent years, Global Navigation Satellite System Reflectometry (GNSS-R) technology has made considerable progress with the increasing of GNSS-R satellites in orbit, the improvements of GNSS-R data processing technology, and the expansion of its ... ...

    Abstract In recent years, Global Navigation Satellite System Reflectometry (GNSS-R) technology has made considerable progress with the increasing of GNSS-R satellites in orbit, the improvements of GNSS-R data processing technology, and the expansion of its geophysical applications. Meanwhile, with the modernization and evolution of GNSS systems, more signal sources and signal modulation modes are available. The effective use of the signals at different frequencies or from new GNSS systems can improve the accuracy, reliability, and resolution of the GNSS-R data products. This paper analyses the signal-to-noise ratio (SNR) of the GNSS-R measurements from Galileo and BeiDou-3 (BDS-3) systems, which is one of the important indicators to measure the quality of GNSS-R data. The multi-GNSS (GPS, Galileo and BDS-3) complex waveform products generated from the raw intermediate frequency data from TechDemoSat-1 (TDS-1) satellite and Cyclone Global Navigation Satellite System (CYGNSS) constellation are used for such analyses. The SNR and normalized SNR (NSNR) of the reflected signals from Galileo and BDS-3 satellites are compared to these from GPS. Preliminary results show that the GNSS-R SNRs from Galileo and BDS-3 are ∼1–2 dB lower than the GNSS-R measurements from GPS, which could be due to the power of the transmitted power and the bandwidth of the receiver. In addition, the effect of coherent integration time on GNSS-R SNR is also assessed for different GNSS signals. It is shown that the SNR of the reflected signals can be improved by using longer coherent integration time (∼0.4–0.8 dB with 2 ms coherent integration and ∼0.6–1.2 dB with 4 ms coherent integration). In addition, it is also shown that the SNR can be improved more efficiently (∼0.2–0.4 dB) for reflected BDS-3 and Galileo signals than for GPS. These results can provide useful references for the design of future spaceborne GNSS-R instrument compatible with reflections from multi-GNSS constellations.
    Keywords Global Navigation Satellite System Reflectometry (GNSS-R) ; multi-GNSS ; complex waveform ; signal-to-noise ratio (SNR) ; coherent integration time ; Science ; Q
    Subject code 551
    Language English
    Publishing date 2022-12-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  8. Article ; Online: Cadmium tolerance and hyperaccumulation in plants – A proteomic perspective of phytoremediation

    Liangjie Niu / Chunyang Li / Wei Wang / Jinghua Zhang / Monica Scali / Weiqiang Li / Hui Liu / Fuju Tai / Xiuli Hu / Xiaolin Wu

    Ecotoxicology and Environmental Safety, Vol 256, Iss , Pp 114882- (2023)

    2023  

    Abstract: Cadmium (Cd) is a major environmental pollutant and poses a risk of transfer into the food chain through contaminated plants. Mechanisms underlying Cd tolerance and hyperaccumulation in plants are not fully understood. Proteomics-based approaches ... ...

    Abstract Cadmium (Cd) is a major environmental pollutant and poses a risk of transfer into the food chain through contaminated plants. Mechanisms underlying Cd tolerance and hyperaccumulation in plants are not fully understood. Proteomics-based approaches facilitate an in-depth understanding of plant responses to Cd stress at the systemic level by identifying Cd-inducible differentially abundant proteins (DAPs). In this review, we summarize studies related to proteomic changes associated with Cd-tolerance mechanisms in Cd-tolerant crops and Cd-hyperaccumulating plants, especially the similarities and differences across plant species. The enhanced DAPs identified through proteomic studies can be potential targets for developing Cd-hyperaccumulators to remediate Cd-contaminated environments and Cd-tolerant crops with low Cd content in the edible organs. This is of great significance for ensuring the food security of an exponentially growing global population. Finally, we discuss the methodological drawbacks in current proteomic studies and propose that better protocols and advanced techniques should be utilized to further strengthen the reliability and applicability of future Cd-stress-related studies in plants. This review provides insights into the improvement of phytoremediation efficiency and an in-depth study of the molecular mechanisms of Cd enrichment in plants.
    Keywords Cadmium (Cd) tolerance ; Differentially abundant proteins (DAPs) ; Heavy metal accumulation ; Proteomics ; Phytoremediation ; Environmental pollution ; TD172-193.5 ; Environmental sciences ; GE1-350
    Subject code 580
    Language English
    Publishing date 2023-05-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Responses of breeding waterbird communities to environmental changes in subsidence wetlands in the North China Plain

    Guangyao Wang / Jinming Zhao / Weiqiang Li / Xiangrong Song / Yong Zhang / Chunlin Li / Willem F. de Boer

    Avian Research, Vol 14, Iss , Pp 100110- (2023)

    2023  

    Abstract: In the context of global degradation and loss of natural wetlands, waterbirds have been increasingly using artificial wetlands as alternative habitats. However, waterbirds are facing various threats in these artificial wetlands, due to dramatic ... ...

    Abstract In the context of global degradation and loss of natural wetlands, waterbirds have been increasingly using artificial wetlands as alternative habitats. However, waterbirds are facing various threats in these artificial wetlands, due to dramatic environmental changes induced by anthropogenic activities. Exploring the effects of these changes on the temporal dynamics of the waterbird communities can help understand how waterbirds adapt to environmental changes and thus formulate effective management and conservation plans. In this study, we carried out field surveys on waterbirds and environmental factors across 20 subsidence wetlands created by underground coal mining in the Huainan coal mining area in the breeding seasons of 2016 and 2021. We predicted that the waterbird assemblages (i.e., number of individuals, species richness, Shannon–Wiener diversity, Pielou evenness and species composition) differed between the two years, and that these differences were correlated with the temporal changes in environmental factors. Across the surveyed wetlands, we recorded 26 waterbird species in 2016 and 23 in 2021. For individual wetlands, the number of waterbird individuals and species richness increased by 71.6% and 20.1%, respectively, over the five years, with no changes in Shannon–Wiener diversity and Pielou evenness. The overall increase in the number of bird individuals was mainly caused by an increase in vegetation gleaners and gulls that adapt well to anthropogenic activities. The species composition was significantly different between the two years, which was mainly caused by changes in the number of individuals of dominant species under influence of changes in human activities. For most wetlands, the temporal pairwise β-diversities could be explained by species turnover rather than nestedness, probably due to high mobility of waterbird species and dramatic changes in local environments. Our study suggests that waterbird communities could respond to environmental changes in subsidence wetlands, providing ...
    Keywords Artificial wetlands ; Environmental changes ; Subsidence wetlands ; Waterbird community ; Zoology ; QL1-991
    Subject code 333
    Language English
    Publishing date 2023-01-01T00:00:00Z
    Publisher KeAi Communications Co., Ltd.
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Numerical Simulation of Cavitation Performance in Engine Cooling Water Pump Based on a Corrected Cavitation Model

    Wei Li / Enda Li / Weidong Shi / Weiqiang Li / Xiwei Xu

    Processes, Vol 8, Iss 3, p

    2020  Volume 278

    Abstract: To analyze the internal flow of the engine cooling water pump (ECWP) under thermodynamic effect, Zwart cavitation model based on the Rayleigh-Plesset equation is corrected, and NACA0015 hydrofoil was selected to verify the corrected model. The cavitation ...

    Abstract To analyze the internal flow of the engine cooling water pump (ECWP) under thermodynamic effect, Zwart cavitation model based on the Rayleigh-Plesset equation is corrected, and NACA0015 hydrofoil was selected to verify the corrected model. The cavitation performances of ECWP with different temperatures were numerically simulated based on a corrected cavitation model. Research results show that simulation values of pressure distribution coefficient in hydrofoil surface at 70 °C are in closest agreement with experimental values when the evaporation and condensation coefficients are 10 and 0.002, respectively. With the decrease of absolute pressure in pump inlet, bubbles firstly occurred at the blade inlet side near the suction surface and then gradually extended to the pressure surface, finally clogged the impeller passage. Compared to the inlet section, the cavitation degree is much more serious close to the trailing edge. With the temperature increases, the cavitation in ECWP occurs in advance and rapidly, and the temperature plays an important role in promoting cavitation process in ECWP. Based on the unsteady simulation of ECWP, the influence of cavitation on the performance characteristics is studied. The results provide a theoretical reference for the prediction and optimization of the cavitation performance in ECWP.
    Keywords cavitation model ; numerical simulation ; engine cooling water pump ; thermodynamic effect ; unsteady process ; Chemical technology ; TP1-1185 ; Chemistry ; QD1-999
    Subject code 518
    Language English
    Publishing date 2020-02-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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