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  1. Article ; Online: Biomanufacturing human tissues via organ building blocks.

    Wolf, Kayla J / Weiss, Jonathan D / Uzel, Sebastien G M / Skylar-Scott, Mark A / Lewis, Jennifer A

    Cell stem cell

    2022  Volume 29, Issue 5, Page(s) 667–677

    Abstract: The construction of human organs on demand remains a tantalizing vision to solve the organ donor shortage. Yet, engineering tissues that recapitulate the cellular and architectural complexity of native organs is a grand challenge. The use of organ ... ...

    Abstract The construction of human organs on demand remains a tantalizing vision to solve the organ donor shortage. Yet, engineering tissues that recapitulate the cellular and architectural complexity of native organs is a grand challenge. The use of organ building blocks (OBBs) composed of multicellular spheroids, organoids, and assembloids offers an important pathway for creating organ-specific tissues with the desired cellular-to-tissue-level organization. Here, we review the differentiation, maturation, and 3D assembly of OBBs into functional human tissues and, ultimately, organs for therapeutic repair and replacement. We also highlight future challenges and areas of opportunity for this nascent field.
    MeSH term(s) Humans ; Organoids ; Spheroids, Cellular ; Tissue Engineering
    Language English
    Publishing date 2022-05-06
    Publishing country United States
    Document type Journal Article ; Review ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2375354-7
    ISSN 1875-9777 ; 1934-5909
    ISSN (online) 1875-9777
    ISSN 1934-5909
    DOI 10.1016/j.stem.2022.04.012
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: A Microwell Cell Capture Device Reveals Variable Response to Dobutamine in Isolated Cardiomyocytes.

    Clark, J Alexander / Weiss, Jonathan D / Campbell, Stuart G

    Biophysical journal

    2019  Volume 117, Issue 7, Page(s) 1258–1268

    Abstract: Isolated ventricular cardiomyocytes exhibit substantial cell-to-cell variability, even when obtained from the same small volume of myocardium. In this study, we investigated the possibility that cardiomyocyte responses to β-adrenergic stimulus are also ... ...

    Abstract Isolated ventricular cardiomyocytes exhibit substantial cell-to-cell variability, even when obtained from the same small volume of myocardium. In this study, we investigated the possibility that cardiomyocyte responses to β-adrenergic stimulus are also highly heterogeneous. To achieve the throughput and measurement duration desired for these experiments, we designed and validated a novel microwell system that immobilizes and uniformly orients isolated adult cardiomyocytes. In this configuration, detailed drug responses of dozens of cells can be followed for intervals exceeding 1 h. At the conclusion of an experiment, specific cells can also be harvested via a precision aspirator for single-cell gene expression profiling. Using this system, we followed changes in Ca
    MeSH term(s) Animals ; Cell Separation/instrumentation ; Dobutamine/pharmacology ; Female ; Gene Expression Regulation/drug effects ; Microtechnology/instrumentation ; Myocytes, Cardiac/cytology ; Myocytes, Cardiac/drug effects ; Myocytes, Cardiac/metabolism ; Rats ; Rats, Sprague-Dawley ; Stochastic Processes
    Chemical Substances Dobutamine (3S12J47372)
    Language English
    Publishing date 2019-08-28
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 218078-9
    ISSN 1542-0086 ; 0006-3495
    ISSN (online) 1542-0086
    ISSN 0006-3495
    DOI 10.1016/j.bpj.2019.08.024
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  3. Article: Using fiber optics to detect moisture intrusion into a landfill cap consisting of a vegetative soil barrier.

    Weiss, Jonathan D

    Journal of the Air & Waste Management Association (1995)

    2003  Volume 53, Issue 9, Page(s) 1130–1148

    Abstract: The intrusion of moisture into landfills can pose a health hazard because of the possibility that the moisture will carry harmful substances into the groundwater. Early detection of moisture anywhere within these landfills is essential if corrective ... ...

    Abstract The intrusion of moisture into landfills can pose a health hazard because of the possibility that the moisture will carry harmful substances into the groundwater. Early detection of moisture anywhere within these landfills is essential if corrective action is to be taken well before an occurrence of this kind. This paper presents the results of a field-scale simulation test of the use of fiber optics to detect the presence of moisture within landfill covers, using a detection method based on the thermal response of soils as a function of their moisture content. By sending electrical current through an embedded stainless-steel tube, soils of varying moisture content were heated and time-dependent temperature measurements were obtained with a fiber-optic distributed temperature sensor system. The optical fiber itself lay within the tube, but its temperature was a function of how rapidly heat was conducted into the surrounding medium. The results of this experiment, which are in agreement with those obtained using more traditional "point" sampling and laboratory analysis, are presented along with the strengths and limitations of the thermal-response method of detecting moisture.
    MeSH term(s) Environmental Monitoring/instrumentation ; Fiber Optic Technology ; Optical Fibers ; Plants ; Refuse Disposal ; Soil ; Soil Pollutants ; Water/analysis ; Water Movements ; Water Pollutants
    Chemical Substances Soil ; Soil Pollutants ; Water Pollutants ; Water (059QF0KO0R)
    Language English
    Publishing date 2003-09
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 1003064-5
    ISSN 1096-2247 ; 0894-0630 ; 1047-3289
    ISSN 1096-2247 ; 0894-0630 ; 1047-3289
    DOI 10.1080/10473289.2003.10466268
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  4. Article ; Online: Large-Scale Production of Wholly Cellular Bioinks via the Optimization of Human Induced Pluripotent Stem Cell Aggregate Culture in Automated Bioreactors.

    Ho, Debbie L L / Lee, Stacey / Du, Jianyi / Weiss, Jonathan D / Tam, Tony / Sinha, Soham / Klinger, Danielle / Devine, Sean / Hamfeldt, Art / Leng, Hope T / Herrmann, Jessica E / He, Mengdi / Fradkin, Lee G / Tan, Tze Kai / Standish, David / Tomasello, Peter / Traul, Donald / Dianat, Noushin / Ladi, Rukmini /
    Vicard, Quentin / Katikireddy, Kishore / Skylar-Scott, Mark A

    Advanced healthcare materials

    2022  Volume 11, Issue 24, Page(s) e2201138

    Abstract: Combining the sustainable culture of billions of human cells and the bioprinting of wholly cellular bioinks offers a pathway toward organ-scale tissue engineering. Traditional 2D culture methods are not inherently scalable due to cost, space, and ... ...

    Abstract Combining the sustainable culture of billions of human cells and the bioprinting of wholly cellular bioinks offers a pathway toward organ-scale tissue engineering. Traditional 2D culture methods are not inherently scalable due to cost, space, and handling constraints. Here, the suspension culture of human induced pluripotent stem cell-derived aggregates (hAs) is optimized using an automated 250 mL stirred tank bioreactor system. Cell yield, aggregate morphology, and pluripotency marker expression are maintained over three serial passages in two distinct cell lines. Furthermore, it is demonstrated that the same optimized parameters can be scaled to an automated 1 L stirred tank bioreactor system. This 4-day culture results in a 16.6- to 20.4-fold expansion of cells, generating approximately 4 billion cells per vessel, while maintaining >94% expression of pluripotency markers. The pluripotent aggregates can be subsequently differentiated into derivatives of the three germ layers, including cardiac aggregates, and vascular, cortical and intestinal organoids. Finally, the aggregates are compacted into a wholly cellular bioink for rheological characterization and 3D bioprinting. The printed hAs are subsequently differentiated into neuronal and vascular tissue. This work demonstrates an optimized suspension culture-to-3D bioprinting pipeline that enables a sustainable approach to billion cell-scale organ engineering.
    MeSH term(s) Humans ; Induced Pluripotent Stem Cells ; Cell Culture Techniques ; Cell Proliferation ; Cell Line ; Bioreactors
    Language English
    Publishing date 2022-11-22
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2649576-4
    ISSN 2192-2659 ; 2192-2640
    ISSN (online) 2192-2659
    ISSN 2192-2640
    DOI 10.1002/adhm.202201138
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