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Article: [Pay more attention to preoperative management of gynecological cancer patients with medical comorbidities].

Wen, H W

2016 Volume 51, Issue 11, Page(s) 801–804

Language	Chinese
Publishing date	2016--25
Publishing country	China
Document type	Journal Article
ZDB-ID	604570-4
ISSN	0529-567X
ISSN	0529-567X
DOI	10.3760/cma.j.issn.0529-567X.2016.11.001
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Zs.A 1619: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 324: Show issues

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Article ; Online: Combined multiplex loop-mediated isothermal amplification with lateral flow assay to detect sea and seb genes of enterotoxic Staphylococcus aureus.

Yin, H Y / Fang, T J / Wen, H W

Letters in applied microbiology

2016 Volume 63, Issue 1, Page(s) 16–24

Abstract: Unlabelled: Staphylococcal enterotoxins (SEs) are the most common cause of food poisoning worldwide and can induce symptoms, such as diarrhoea, vomiting and abdominal cramping. Thus, the aim of this study is to develop a multiplex loop-mediated ... ...

Abstract	Unlabelled: Staphylococcal enterotoxins (SEs) are the most common cause of food poisoning worldwide and can induce symptoms, such as diarrhoea, vomiting and abdominal cramping. Thus, the aim of this study is to develop a multiplex loop-mediated isothermal amplification combined with a lateral flow assay (m-LAMP/LFA) to simultaneously detect the sea and seb genes of Staphylococcus aureus. The amplicons of the sea gene were labelled with digoxigenin (Dig) and biotin while those of seb gene were labelled with fluorescein isothiocyanate (FITC) and biotin. These amplicons were detected using a multiplex LFA with NeutrAvidin-tagged gold nanoparticles as the detection reagent. After optimization, the detection limit of this assay was 10(2) CFU ml(-1) Staph. aureus, which was one tenth that of a multiplex PCR. This assay did not exhibit any cross-reactivity in detecting other enterotoxic Staph. aureus strains or other food pathogens. After 6 h of enrichment, this developed assay detected 1 CFU ml(-1) of Staph. aureus in milk, apple juice, cheese and rice. The developed m-LAMP/LFA method does not require expensive equipment and can be completely implemented within an 8-h workday. Therefore, this method can provide an effective means of quickly screening staphylococcal enterotoxin A- and/or staphylococcal enterotoxin B-producing Staph. aureus in food samples. Significance and impact of the study: Staphylococcus aureus is one of the major foodborne pathogens worldwide, and its staphylococcal enterotoxin A and B are strongly associated with food poisoning. This work developed a multiplex loop-mediated isothermal amplification combined with a lateral flow assay (m-LAMP/LFA) to simultaneously detect the sea and seb genes of Staph. aureus in food samples. The assay has good specificity and sensitivity with ease-of-use features, making it ideal for on-site detection.
MeSH term(s)	Animals ; Cheese/microbiology ; Enterotoxins/genetics ; Food Microbiology ; Fruit and Vegetable Juices/microbiology ; Limit of Detection ; Milk/microbiology ; Multiplex Polymerase Chain Reaction/methods ; Nucleic Acid Amplification Techniques/methods ; Oryza/microbiology ; Sensitivity and Specificity ; Staphylococcus aureus/genetics ; Staphylococcus aureus/pathogenicity
Chemical Substances	Enterotoxins ; enterotoxin A, Staphylococcal (37337-57-8) ; enterotoxin B, staphylococcal (39424-53-8)
Language	English
Publishing date	2016-07
Publishing country	England
Document type	Journal Article
ZDB-ID	632584-1
ISSN	1472-765X ; 0266-8254
ISSN (online)	1472-765X
ISSN	0266-8254
DOI	10.1111/lam.12590
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Article: [Perioperational management of gynecological cancer patients with severe internal medical complications: a serial of 37 clinical cases].

Hu, J / Lyu, W Q / Guo, Y L / Wen, H W / Qiao, H / Qu, Y

Zhonghua fu chan ke za zhi

2016 Volume 51, Issue 11, Page(s) 805–809

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Aged ; Anesthesia/adverse effects ; Anesthesia/mortality ; Comorbidity ; Coronary Disease/epidemiology ; Diabetes Mellitus/epidemiology ; Female ; Genital Neoplasms, Female/epidemiology ; Genital Neoplasms, Female/pathology ; Genital Neoplasms, Female/surgery ; Humans ; Hypertension/epidemiology ; Middle Aged ; Perioperative Care ; Perioperative Period ; Postoperative Complications/epidemiology ; Postoperative Complications/mortality ; Retrospective Studies
Language	Chinese
Publishing date	2016-11-25
Publishing country	China
Document type	Evaluation Studies ; Journal Article
ZDB-ID	604570-4
ISSN	0529-567X
ISSN	0529-567X
DOI	10.3760/cma.j.issn.0529-567X.2016.11.002
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Zs.A 1619: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
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Article ; Online: The feasibility of QR-code prescription in Taiwan.

Lin, C-H / Tsai, F-Y / Tsai, W-L / Wen, H-W / Hu, M-L

Journal of clinical pharmacy and therapeutics

2012 Volume 37, Issue 6, Page(s) 643–646

Abstract: What is known and objective: An ideal Health Care Service is a service system that focuses on patients. Patients in Taiwan have the freedom to fill their prescriptions at any pharmacies contracted with National Health Insurance. Each of these pharmacies ...

Abstract	What is known and objective: An ideal Health Care Service is a service system that focuses on patients. Patients in Taiwan have the freedom to fill their prescriptions at any pharmacies contracted with National Health Insurance. Each of these pharmacies uses its own computer system. So far, there are at least ten different systems on the market in Taiwan. To transmit the prescription information from the hospital to the pharmacy accurately and efficiently presents a great issue. Methods: This study consisted of two-dimensional applications using a QR-code to capture Patient's identification and prescription information from the hospitals as well as using a webcam to read the QR-code and transfer all data to the pharmacy computer system. Two hospitals and 85 community pharmacies participated in the study. Results and discussion: During the trial, all participant pharmacies appraised highly of the accurate transmission of the prescription information. The contents in QR-code prescriptions from Taipei area were picked up efficiently and accurately in pharmacies at Taichung area (middle Taiwan) without software system limit and area limitation. The QR-code device received a patent (No. M376844, March 2010) from Intellectual Property Office Ministry of Economic Affair, China. What is new and conclusion: Our trial has proven that QR-code prescription can provide community pharmacists an efficient, accurate and inexpensive device to digitalize the prescription contents. Consequently, pharmacists can offer better quality of pharmacy service to patients.
MeSH term(s)	Automatic Data Processing ; Clinical Pharmacy Information Systems ; Community Pharmacy Services/organization & administration ; Community Pharmacy Services/standards ; Data Collection ; Drug Prescriptions ; Feasibility Studies ; Humans ; National Health Programs/organization & administration ; Pharmacists/organization & administration ; Pharmacists/standards ; Pharmacy Service, Hospital/organization & administration ; Pharmacy Service, Hospital/standards ; Professional Role ; Quality Assurance, Health Care ; Software ; Taiwan
Language	English
Publishing date	2012-12
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	639006-7
ISSN	1365-2710 ; 0269-4727
ISSN (online)	1365-2710
ISSN	0269-4727
DOI	10.1111/j.1365-2710.2012.01358.x
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Article ; Online: Unequal coupling between locomotor pacemakers of the German cockroach, Blattella germanica (L.).

Wen, H -W. / Lee, H -J.

Journal of insect physiology

2000 Volume 46, Issue 1, Page(s) 89–97

Abstract: Male adult German cockroaches, Blattella germanica (L.), expressed robust locomotor circadian rhythmicity under 28 degrees C and constant darkness (DD) conditions. By surgically severing the connections between the optic lobes and midbrain and their ... ...

Abstract	Male adult German cockroaches, Blattella germanica (L.), expressed robust locomotor circadian rhythmicity under 28 degrees C and constant darkness (DD) conditions. By surgically severing the connections between the optic lobes and midbrain and their subsequent regeneration, we demonstrated that the locomotor circadian pacemaker was located in the optic lobes and that it controlled the locomotor circadian rhythm through neural pathways. From the results that unilaterally optic tract severed males still showed locomotor circadian rhythmicity (93.1%, n=29) without significantly changing the circadian period (tau) or level of motor activity, we concluded that the right and left optic lobes each contain a circadian pacemaker competent to drive the locomotor circadian rhythm. These two pacemakers were strongly coupled since only one rhythm was expressed when the pacemakers were independently exposed to opposite lighting conditions (DD or LL) at the same time. However, an unequal contribution of each pacemaker in determining the overt circadian period was found under constant dim light (10 lux) conditions, revealing a major-minor coupling relationship between these two pacemakers, so that the unilaterally blinded male expressed either a LL-rhythm with a circadian period of 24.27+/-0.21 h (41.7%, n=24) or a DD-rhythm with a circadian period of 23.43+/-0.19 h (58.3%, n=24). However, higher intensity of photic information (200-300 lux) could overpower this relationship and cause the minor pacemaker to lead the rhythm.
Language	English
Publishing date	2000-01
Publishing country	England
Document type	Journal Article
ISSN	1879-1611
ISSN (online)	1879-1611
DOI	10.1016/s0022-1910(99)00105-5
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Article: [Prognostic factors in patients with stage I epithelial ovarian cancer].

Wen, H W / Liu, S W

Zhonghua fu chan ke za zhi

1994 Volume 29, Issue 4, Page(s) 235–7, 255

Abstract: From 1962 to 1989, 67 patients with stage I epithelial ovarian cancer were treated in our hospital. They were divided into three groups according to the time period of treatment. Thirteen patients treated in the 1960s were in group 1, 23 in the 1970s in ... ...

Abstract	From 1962 to 1989, 67 patients with stage I epithelial ovarian cancer were treated in our hospital. They were divided into three groups according to the time period of treatment. Thirteen patients treated in the 1960s were in group 1, 23 in the 1970s in group 2, and 31 in 1980s in group 3. There were no other significant differences other than the adjuvant chemotherapy in the three groups. In this study, the 5-year survival rate was 46% in group 1 (1962-1969), 82% in group 2 (1970-1979), and 97% in group 3 (1980-1989). The prognosis was improved significantly from 1960s to 1970s (P < 0.005), as well as from 1970s to 1980s (P < 0.05). Multivariate analysis indicated adjuvant chemotherapy (P < 0.01), stage (P < 0.01), and histological grade (P < 0.05) as having independent prognostic effects. Patients with more than 4 courses of combination chemotherapy, stage Ia, grade I and grade II had a better survival rate. The chemotherapy, complete surgical staging and preservation of fertility in surgical treatment of stage I epithelial ovarian cancer were also discussed.
MeSH term(s)	Adult ; Aged ; Carcinoma/mortality ; Carcinoma/pathology ; Carcinoma/therapy ; Chemotherapy, Adjuvant ; China/epidemiology ; Female ; Humans ; Middle Aged ; Neoplasm Staging ; Ovarian Neoplasms/mortality ; Ovarian Neoplasms/pathology ; Ovarian Neoplasms/therapy ; Prognosis ; Retrospective Studies ; Survival Rate
Language	Chinese
Publishing date	1994-04
Publishing country	China
Document type	Comparative Study ; English Abstract ; Journal Article
ZDB-ID	604570-4
ISSN	0529-567X
ISSN	0529-567X
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Zs.A 1619: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 324: Show issues

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Article: [48 years malaria control in Xuchang City].

Wen, H W / Han, C Y / Han, Q Y

Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases

2001 Volume 19, Issue 3, Page(s) 185

MeSH term(s)	China/epidemiology ; Humans ; Malaria/epidemiology ; Malaria/prevention & control ; Mosquito Control ; Population Surveillance
Language	Chinese
Publishing date	2001
Publishing country	China
Document type	Journal Article
ZDB-ID	639272-6
ISSN	1000-7423
ISSN	1000-7423
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Article: Constructing liposomal nanovesicles of ginseng extract against hydrogen peroxide-induced oxidative damage to L929 cells

Tsai, W.-C. / Li, W.-C. / Yin, H.-Y. / Yu, M.-C. / Wen, H.-W.

Food chemistry

2012 Volume 132, Issue 2, Page(s) 744

Language	English
Document type	Article
ZDB-ID	243123-3
ISSN	0308-8146
Database	Current Contents Nutrition, Environment, Agriculture

In stock of ZB MED Bonn / Germany

Z 3634: Show issues

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Article: Unequal coupling between locomotor pacemakers of the German cockroach, Blattella germanica (L.)

Wen, H.W / Lee, H.J

Journal of insect physiology. Jan 2000. v. 46 (1)

2000

Abstract: Male adult German cockroaches, Blattella germanica (L.), expressed robust locomotor circadian rhythmicity under 28 degree C and constant darkness (DD) conditions. By surgically severing the connections between the optic lobes and midbrain and their ... ...

Abstract	Male adult German cockroaches, Blattella germanica (L.), expressed robust locomotor circadian rhythmicity under 28 degree C and constant darkness (DD) conditions. By surgically severing the connections between the optic lobes and midbrain and their subsequent regeneration, we demonstrated that the locomotor circadian pacemaker was located in the optic lobes and that it controlled the locomotor circadian rhythm through neural pathways. From the results that unilaterally optic tract severed males still showed locomotor circadian rhythmicity (93.1%, n=29) without significantly changing the circadian period (tau) or level of motor activity, we concluded that the right and left optic lobes each contain a circadian pacemaker competent to drive the locomotor circadian rhythm. These two pacemakers were strongly coupled since only one rhythm was expressed when the pacemakers were independently exposed to opposite lighting conditions (DD or LL) at the same time. However, an unequal contribution of each pacemaker in determining the overt circadian period was found under constant dim light (10 lux) conditions, revealing a major-minor coupling relationship between these two pacemakers, so that the unilaterally blinded male expressed either a LL-rhythm with a circadian period of 24.27 +/- 0.21 h (41.7%, n=24) or a DD-rhythm with a circadian period of 23.43 +/- 0.19 h (58.3%, n=24).However, higher intensity of photic information (200-300 lux) could overpower this relationship and cause the minor pacemaker to lead the rhythm.
Keywords	Blattella germanica ; light intensity ; circadian rhythm ; locomotion ; brain ; neurophysiology ; peripheral nerves
Language	English
Dates of publication	2000-01
Size	p. 89-97.
Document type	Article
ISSN	0022-1910
Database	NAL-Catalogue (AGRICOLA)

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Article: A novel extraction method for peanut allergenic proteins in chocolate and their detection by a liposome-based lateral flow assay

Wen, H.W / Borejsza-Wysocki, W / DeCory, T.R / Baeumner, A.J / Durst, R.A

European food research and technology = Zeitschrift fur Lebensmittel-Untersuchung und -Forschung. A. 2005 Aug., v. 221, no. 3-4

2005

Keywords	chocolate ; peanuts ; allergens ; plant proteins ; extraction ; liposomes (artificial) ; food contamination ; food analysis ; optimization ; hexane ; saline water ; solvents
Language	English
Dates of publication	2005-08
Size	p. 564-569.
Document type	Article
ZDB-ID	1359456-4
ISSN	1431-4630 ; 1438-2377
ISSN	1431-4630 ; 1438-2377
Database	NAL-Catalogue (AGRICOLA)

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Z 792: Show issues

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