LIVIVO - Search results -

Search results

Result 1 - 9 of total 9

Search options

Article ; Online: Du Huo Ji Sheng Tang inhibits Notch1 signaling and subsequent NLRP3 activation to alleviate cartilage degradation in KOA mice

Wen-jin Chen / Yin Zhuang / Wei Peng / Wei Cui / Shu-jun Zhang / Jian-wei Wang

Chinese Medicine, Vol 18, Iss 1, Pp 1-

2023 Volume 15

Abstract: Abstract Background Knee osteoarthritis (KOA) has a complex pathological mechanism and is difficult to cure. The traditional medicine Du Huo Ji Sheng Tang (DHJST) has been used for the treatment of KOA for more than one thousand years, but its mechanism ... ...

Abstract	Abstract Background Knee osteoarthritis (KOA) has a complex pathological mechanism and is difficult to cure. The traditional medicine Du Huo Ji Sheng Tang (DHJST) has been used for the treatment of KOA for more than one thousand years, but its mechanism for treating KOA has not been revealed. In our previous study, we confirmed that DHJST inhibited the activation of NLRP3 signaling in rats and humans. In the current study, we aimed to determine how DHJST inhibits NLRP3 to alleviate knee cartilage damage. Methods Mice were injected with NLRP3 shRNA or Notch1-overexpressing adenovirus into the tail vein to construct systemic NLRP3 low-expressing or Notch1 high-expressing mice. Mice were injected with papain into the knee joint to replicate the KOA model. DHJST was used to treat KOA model mice with different backgrounds. The thickness of the right paw was measured to evaluate toe swelling. The pathohistological changes and the levels of IL-1β, MMP2, NLRP3, Notch1, collagen 2, collagen 4, HES1, HEY1, and Caspase3 were detected by HE staining, ELISA, immunohistochemical staining, western blotting, or real-time qPCR. Results DHJST reduced tissue swelling and serum and knee cartilage IL-1β levels, inhibited cartilage MMP2 expression, increased collagen 2 and collagen 4 levels, decreased Notch1 and NLRP3 positive expression rates in cartilage, and decreased HES1 and HEY1 mRNA levels in KOA model mice. In addition, NLRP3 interference decreased cartilage MMP2 expression and increased collagen 2 and collagen 4 levels without affecting the expression levels of notch1, HES1 and HEY1 mRNA levels in the synovium of KOA mice. In KOA mice with NLRP interference, DHJST further reduced tissue swelling and knee cartilage damage in mice. Finally, Notch1-overexpressing mice not only showed more severe tissue swelling and knee cartilage degradation but also abolished the therapeutic effect of DHJST on KOA mice. Importantly, the inhibitory effects of DHJST on the mRNA expression of NLRP3, Caspase3 and IL-1β in the knee joint of KOA ...
Keywords	Osteoarthritis ; DHJST ; NLRP3 ; Notch1 ; Mice ; Inflammation ; Other systems of medicine ; RZ201-999
Subject code	616
Language	English
Publishing date	2023-06-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Microsurgery for Vestibular Schwannoma via Retrosigmoid Transmeatal Approach with Intraoperative Monitoring Techniques

Li-Hua Chen / Hong-Tian Zhang / Kai Sun / Wen-Jin Chen / Ru-Xiang Xu

Balkan Medical Journal, Vol 38, Iss 4, Pp 212-

2021 Volume 221

Keywords	Medicine ; R
Language	English
Publishing date	2021-07-01T00:00:00Z
Publisher	Galenos Publishing House
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Molecular subtyping and characterization of clear cell renal cell carcinoma by tumor differentiation trajectories

Xiu-wu Pan / Wen-jin Chen / Da Xu / Wen-bin Guan / Lin Li / Jia-xin Chen / Wei-jie Chen / Ke-qin Dong / Jian-qing Ye / Si-shun Gan / Wang Zhou / Xin-gang Cui

iScience, Vol 26, Iss 12, Pp 108370- (2023)

2023

Abstract: Summary: Previous bulk RNA sequencing or whole genome sequencing on clear cell renal cell carcinoma (ccRCC) subtyping mainly focused on ccRCC cell origin or the complex tumor microenvironment (TME). Based on the single-cell RNA sequencing (scRNA-seq) ... ...

Abstract	Summary: Previous bulk RNA sequencing or whole genome sequencing on clear cell renal cell carcinoma (ccRCC) subtyping mainly focused on ccRCC cell origin or the complex tumor microenvironment (TME). Based on the single-cell RNA sequencing (scRNA-seq) data of 11 primary ccRCC specimens, cancer stem-cell-like subsets could be differentiated into five trajectories, whereby we further classified ccRCC cells into three groups with diverse molecular features. These three ccRCC subgroups showed significantly different outcomes and potential targets to tyrosine kinase inhibitors (TKIs) or immune checkpoint inhibitors (ICIs). Tumor cells in three differentiation directions exhibited distinct interactions with other subsets in the ccRCC niches. The subtyping model was examined through immunohistochemistry staining in our ccRCC cohort and validated the same classification effect as the public patients. All these findings help gain a deeper understanding about the pathogenesis of ccRCC and provide useful clues for optimizing therapeutic schemes based on the molecular subtype analysis.
Keywords	Molecular mechanism of gene regulation ; Cancer ; Transcriptomics ; Science ; Q
Subject code	616
Language	English
Publishing date	2023-12-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: TRIM32 Deficiency Impairs the Generation of Pyramidal Neurons in Developing Cerebral Cortex

Yan-Yun Sun / Wen-Jin Chen / Ze-Ping Huang / Gang Yang / Ming-Lei Wu / De-En Xu / Wu-Lin Yang / Yong-Chun Luo / Zhi-Cheng Xiao / Ru-Xiang Xu / Quan-Hong Ma

Cells, Vol 11, Iss 449, p

2022 Volume 449

Abstract: Excitatory-inhibitory imbalance (E/I) is a fundamental mechanism underlying autism spectrum disorders (ASD). TRIM32 is a risk gene genetically associated with ASD. The absence of TRIM32 causes impaired generation of inhibitory GABAergic interneurons, ... ...

Abstract	Excitatory-inhibitory imbalance (E/I) is a fundamental mechanism underlying autism spectrum disorders (ASD). TRIM32 is a risk gene genetically associated with ASD. The absence of TRIM32 causes impaired generation of inhibitory GABAergic interneurons, neural network hyperexcitability, and autism-like behavior in mice, emphasizing the role of TRIM32 in maintaining E/I balance, but despite the description of TRIM32 in regulating proliferation and differentiation of cultured mouse neural progenitor cells (NPCs), the role of TRIM32 in cerebral cortical development, particularly in the production of excitatory pyramidal neurons, remains unknown. The present study observed that TRIM32 deficiency resulted in decreased numbers of distinct layer-specific cortical neurons and decreased radial glial cell (RGC) and intermediate progenitor cell (IPC) pool size. We further demonstrated that TRIM32 deficiency impairs self-renewal of RGCs and IPCs as indicated by decreased proliferation and mitosis. A TRIM32 deficiency also affects or influences the formation of cortical neurons. As a result, TRIM32-deficient mice showed smaller brain size. At the molecular level, RNAseq analysis indicated reduced Notch signalling in TRIM32-deficient mice. Therefore, the present study indicates a role for TRIM32 in pyramidal neuron generation. Impaired generation of excitatory pyramidal neurons may explain the hyperexcitability observed in TRIM32-deficient mice.
Keywords	TRIM32 ; excitatory-inhibitory imbalance ; cortex development ; ASD ; NPCs ; Biology (General) ; QH301-705.5
Subject code	571
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Fully Automated Vortex-Assisted Liquid-Liquid Microextraction Coupled to Gas Chromatography-Mass Spectrometry for the Determination of Trace Levels of Phthalate Esters in Liquor Samples

Leng, Geng / Wen-Jin Chen / Wen-Bo Xu / Yong Wang

Food analytical methods. 2017 Sept., v. 10, no. 9

2017

Abstract: A fully automated vortex-assisted liquid-liquid microextraction (VALLME) system coupled to gas chromatography-mass spectrometry (GC/MS) was developed for the determination of phthalate esters (PAEs) in liquor samples. The whole analytical procedure, ... ...

Abstract	A fully automated vortex-assisted liquid-liquid microextraction (VALLME) system coupled to gas chromatography-mass spectrometry (GC/MS) was developed for the determination of phthalate esters (PAEs) in liquor samples. The whole analytical procedure, including spiking, extraction, phase separation, extractant collection, and GC/MS quantification, were automatically carried out. The use of a commercially available sample vial and a multipurpose sampler equipped with a highly efficient orbital vortex shaker facilitated the accessibility and automation of the method. Key factors, such as type and volume of the extractant, time and speed for VALLME, agitation speed and time required for phase separation, sample pH, salt effects, and matrix effects, were thoroughly investigated. Under the optimum conditions, linearity was in the range 0.05 to 120Â Î¼gÂ Lâ»Â¹. Limits of detection ranged from 0.003 to 0.006Â Î¼gÂ Lâ»Â¹. Enrichment factors were in the range 211 to 304. Reproducibility and recoveries were assessed by testing a series of liquor samples spiked with different concentrations of phthalate esters. This work provided an innovative way to automate the VALLME method and couple it on-line with GC/MS.
Keywords	agitation ; automation ; detection limit ; gas chromatography-mass spectrometry ; liquid-phase microextraction ; pH ; phthalates ; separation
Language	English
Dates of publication	2017-09
Size	p. 3071-3078.
Publishing place	Springer US
Document type	Article
ZDB-ID	2429656-9
ISSN	1936-976X ; 1936-9751
ISSN (online)	1936-976X
ISSN	1936-9751
DOI	10.1007/s12161-017-0874-6
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 7787: Show issues

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Effect of parasitism on food webs

WenJun Zhang / LiQin Jiang / WenJin Chen

Network Biology, Vol 4, Iss 4, Pp 170-

Topological analysis and goodness test of cascade model

2014 Volume 178

Abstract: In this study, we used Pajek to analyze the effect of parasitism on food webs collected from Carpinteria Salt Marsh (CSM). Results showed that median and mean of generality and vulnerability for predator-prey and parasite-host sub-webs were greater than ... ...

Abstract	In this study, we used Pajek to analyze the effect of parasitism on food webs collected from Carpinteria Salt Marsh (CSM). Results showed that median and mean of generality and vulnerability for predator-prey and parasite-host sub-webs were greater than the reported previously. Inclusion of parasites significantly increased the mean generality and vulnerability of the full food web. Effectiveness of cascade model was tested using CSM and arthropod food webs. The results demonstrated that fitting goodness on the predator-prey sub-web without parasites was lower than that on the full CSM food web. Also, cascade model performed worse in fitting arthropod food webs.
Keywords	food webs ; parasites ; Pajek ; network analysis ; cascade model ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2014-12-01T00:00:00Z
Publisher	International Academy of Ecology and Environmental Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Study of cellular heterogeneity and differential dynamics of autophagy in human embryonic kidney development by single-cell RNA sequencing.

Wen-Jin, Chen / Xiu-Wu, Pan / Jian, Chu / Da, Xu / Jia-Xin, Chen / Wei-Jie, Chen / Lin-Hui, Wang / Xin-Gang, Cui

Cancer cell international

2021 Volume 21, Issue 1, Page(s) 460

Abstract: Background: Autophagy is believed to participate in embryonic development, but whether the expression of autophagy-associated genes undergoes changes during the development of human embryonic kidneys remains unknown.: Methods: In this work, we ... ...

Abstract	Background: Autophagy is believed to participate in embryonic development, but whether the expression of autophagy-associated genes undergoes changes during the development of human embryonic kidneys remains unknown. Methods: In this work, we identified 36,151 human renal cells from embryonic kidneys of 9-18 gestational weeks in 16 major clusters by single-cell RNA sequencing (scRNA-seq), and detected 1350 autophagy-related genes in all fetal renal cells. The abundance of each cell cluster in Wilms tumor samples from scRNA-seq and GDC TARGET WT datasets was detected by CIBERSORTx. R package Monocle 3 was used to determine differentiation trajectories. Cyclone tool of R package scran was applied to calculate the cell cycle scores. R package SCENIC was used to investigate the transcriptional regulons. The FindMarkers tool from Seurat was used to calculate DEGs. GSVA was used to perform gene set enrichment analyses. CellphoneDB was utilized to analyze intercellular communication. Results: It was found that cells in the 13th gestational week showed the lowest transcriptional level in each cluster in all stages. Nephron progenitors could be divided into four subgroups with diverse levels of autophagy corresponding to different SIX2 expressions. SSBpod (podocyte precursors) could differentiate into four types of podocytes (Pod), and autophagy-related regulation was involved in this process. Pseudotime analysis showed that interstitial progenitor cells (IPCs) potentially possessed two primitive directions of differentiation to interstitial cells with different expressions of autophagy. It was found that NPCs, pretubular aggregates and interstitial cell clusters had high abundance in Wilms tumor as compared with para-tumor samples with active intercellular communication. Conclusions: All these findings suggest that autophagy may be involved in the development and cellular heterogeneity of early human fetal kidneys. In addition, part of Wilms tumor cancer cells possess the characteristics of some fetal renal cell clusters.
Language	English
Publishing date	2021-08-30
Publishing country	England
Document type	Journal Article
ISSN	1475-2867
ISSN	1475-2867
DOI	10.1186/s12935-021-02154-w
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: A simple field method for the determination of sulfite in natural waters: Based on automated dispersive liquid-liquid microextraction coupled with ultraviolet-visible spectrophotometry

Leng, Geng / Qiong Hu / Wen-Feng He / Zhe Liu / Wen-Jin Chen / Wen-Bo Xu / Qiu-Hong Yang / Jing Sun

Journal of chromatography. 2019 Jan. 11, v. 1584

2019

Abstract: Sulfite is known to be harmful to human health and associated to sulfur related environmental effects and ideally should be analyzed onsite owing to its instability. Here we describe an automated, miniaturized, and highly efficient dispersive liquid- ... ...

Abstract	Sulfite is known to be harmful to human health and associated to sulfur related environmental effects and ideally should be analyzed onsite owing to its instability. Here we describe an automated, miniaturized, and highly efficient dispersive liquid-liquid microextraction (DLLME) system that seamlessly coupled to a UV–vis spectrophotometer for the trace analysis of sulfite in natural waters. The automated DLLME system was constructed by a single syringe pump that is coupled with a multiposition valve. Nanomolar levels of sulfite could be extracted from natural water samples and injected into the hyphenated spectrophotometer for quantification. The whole analytical procedures, including chromogenic reactions, DLLME, collecting and transferring of microvolume of extracts, and spectrophotometric quantification, were automatically carried out. Key parameters that affect the performance of the method were investigated. The method allows the determination of trace levels of sulfite in the range of 15–1500 nM with a detection limit of 1.2 nM. Good reproducibility and recoveries were obtained by analyzing a series of natural water samples that were spiked with different concentration levels. The method was successfully applied to real natural water samples with satisfactory results. The proposed analytical system is light (3.9 kg), simple to use, able to be applied in the field, and sensitive enough for fresh and saline waters analysis.
Keywords	automation ; chromatography ; environmental impact ; field methods ; human health ; liquid-phase microextraction ; saline water ; spectrophotometers ; sulfites ; sulfur ; ultraviolet-visible spectroscopy
Language	English
Dates of publication	2019-0111
Size	p. 72-79.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	218139-3
ISSN	0021-9673 ; 0378-4355 ; 0376-737X
ISSN	0021-9673 ; 0378-4355 ; 0376-737X
DOI	10.1016/j.chroma.2018.11.017
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Cologne/Königswinter

Zs.A 813: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Lung-protective Ventilation in Patients with Brain Injury

Xu-Ying Luo / Ying-Hong Hu / Xiang-Yuan Cao / Yan Kang / Li-Ping Liu / Shou-Hong Wang / Rong-Guo Yu / Xiang-You Yu / Xia Zhang / Bao-Shan Li / Zeng-Xiang Ma / Yi-Bing Weng / Heng Zhang / De-Chang Chen / Wei Chen / Wen-Jin Chen / Xiu-Mei Chen / Bin Du / Mei-Li Duan /

Jin Hu / Yun-Feng Huang / Gui-Jun Jia / Li-Hong Li / Yu-Min Liang / Bing-Yu Qin / Xian-Dong Wang / Jian Xiong / Li-Mei Yan / Zheng-Ping Yang / Chen-Ming Dong / Dong-Xin Wang / Qing-Yuan Zhan / Shuang-Lin Fu / Lin Zhao / Qi-Bing Huang / Ying-Guang Xie / Xiao-Bo Huang / Guo-Bin Zhang / Wang-Bin Xu / Yuan Xu / Ya-Ling Liu / He-Ling Zhao / Rong-Qing Sun / Ming Sun / Qing-Hong Cheng / Xin Qu / Xiao-Feng Yang / Ming Xu / Zhong-Hua Shi / Han Chen

Chinese Medical Journal, Vol 129, Iss 14, Pp 1643-

A Multicenter Cross-sectional Study and Questionnaire Survey in China

2016 Volume 1651

Abstract: Background: Over the years, the mechanical ventilation (MV) strategy has changed worldwide. The aim of the present study was to describe the ventilation practices, particularly lung-protective ventilation (LPV), among brain-injured patients in China. ... ...

Abstract	Background: Over the years, the mechanical ventilation (MV) strategy has changed worldwide. The aim of the present study was to describe the ventilation practices, particularly lung-protective ventilation (LPV), among brain-injured patients in China. Methods: This study was a multicenter, 1-day, cross-sectional study in 47 Intensive Care Units (ICUs) across China. Mechanically ventilated patients (18 years and older) with brain injury in a participating ICU during the time of the study, including traumatic brain injury, stroke, postoperation with intracranial tumor, hypoxic-ischemic encephalopathy, intracranial infection, and idiopathic epilepsy, were enrolled. Demographic data, primary diagnoses, indications for MV, MV modes and settings, and prognoses on the 60th day were collected. Multivariable logistic analysis was used to assess factors that might affect the use of LPV. Results: A total of 104 patients were enrolled in the present study, 87 (83.7%) of whom were identified with severe brain injury based on a Glasgow Coma Scale ≤8 points. Synchronized intermittent mandatory ventilation (SIMV) was the most frequent ventilator mode, accounting for 46.2% of the entire cohort. The median tidal volume was set to 8.0 ml/kg (interquartile range [IQR], 7.0–8.9 ml/kg) of the predicted body weight; 50 (48.1%) patients received LPV. The median positive end-expiratory pressure (PEEP) was set to 5 cmH2O (IQR, 5–6 cmH2O). No PEEP values were higher than 10 cmH2O. Compared with partially mandatory ventilation, supportive and spontaneous ventilation practices were associated with LPV. There were no significant differences in mortality and MV duration between patients subjected to LPV and those were not. Conclusions: Among brain-injured patients in China, SIMV was the most frequent ventilation mode. Nearly one-half of the brain-injured patients received LPV. Patients under supportive and spontaneous ventilation were more likely to receive LPV. Trial Registration: ClinicalTrials.org NCT02517073 ...
Keywords	Brain Injury ; Epidemiology ; Lung-protective Ventilation ; Mechanical Ventilation ; Medicine ; R
Subject code	616 ; 610
Language	English
Publishing date	2016-01-01T00:00:00Z
Publisher	Wolters Kluwer
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED