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  1. Article ; Online: Meta-Analysis of Mechanism of Influence of CRY2 on the Differentiation of Mouse Osteoblast through the Regulation of Wnt/Β-Catenin Signaling Pathway

    Chao Ma / Chaojian Yang / Tong Xie / Wenjuan Dai / Jun Ma

    BioMed Research International, Vol

    2022  Volume 2022

    Abstract: Noncoding RNAs were discovered to control a variety of developmental mechanisms, including osteogenesis. According to emerging evidence, cryptochrome circadian-regulating (CRY) proteins have emerged as essential controllers of osteoblast differentiation. ...

    Abstract Noncoding RNAs were discovered to control a variety of developmental mechanisms, including osteogenesis. According to emerging evidence, cryptochrome circadian-regulating (CRY) proteins have emerged as essential controllers of osteoblast differentiation. The linked processes, on the other hand, are still unknown. The specific process that underpins osteoblast differentiation and proliferation is yet unknown. This research gives a meta-analysis of CRY2’s impact on mouse osteoblast differentiation via the control of the WNT/β–catenin signaling pathways. Western blot and quantitative real-time PCR were used to identify Cry2 expression levels, components in the osteoblast-associated signaling pathway, and osteoblast transcription markers. The osteogenic condition was measured utilizing alkaline phosphatase (ALP) and alizarin red (AR) staining, whereas cell growth rates were measured using CCK8 assays. An ectopic bone formation experiment was used to determine the osteogenic potential of osteoblasts. Cry2 stimulates the osteogenic development of mouse osteoblasts through canonical Wnt/β-catenin signaling, according to the findings.
    Keywords Medicine ; R
    Subject code 570
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher Hindawi Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Downregulation of exosomal CLEC3B in hepatocellular carcinoma promotes metastasis and angiogenesis via AMPK and VEGF signals

    Wenjuan Dai / Yilin Wang / Tianxiao Yang / Jing Wang / Weicheng Wu / Jianxin Gu

    Cell Communication and Signaling, Vol 17, Iss 1, Pp 1-

    2019  Volume 17

    Abstract: Abstract Background C-Type Lectin Domain Family 3 Member B (CLEC3B), is down-regulated in serum and tumor tissues in different cancers including hepatocellular carcinoma (HCC). However, the functions of CLEC3B in HCC remains elucidated. The aim of this ... ...

    Abstract Abstract Background C-Type Lectin Domain Family 3 Member B (CLEC3B), is down-regulated in serum and tumor tissues in different cancers including hepatocellular carcinoma (HCC). However, the functions of CLEC3B in HCC remains elucidated. The aim of this study is to analyze the roles of CLEC3B in HCC. Methods The expression of genes was evaluated by immunohistochemistry, western blot, real-time PCR, enzyme-linked immunosorbent assays, and analysis on TCGA-LIHC database and gene expression omnibus. Transmission electron microscopy and immunofluorescence were applied to detect CLEC3B in exosomes. The function of exosomal CLEC3B in tumor progression were performed in vivo and in vitro. Results We determined that down-regulated CLEC3B in HCC indicated a poor prognosis. Exosomes derived from HCC with down-regulated CLEC3B promoted migration, invasion, epithelial–mesenchymal transition of both tumor cells and endothelial cells (ECs). Moreover, the downregulation CLEC3B in exosomes suppressed VEGF secretion in both HCC cells and ECs, and eventually inhibited angiogenesis. Mechanistically, CLEC3B-mediated VEGF expression in tumor cells and ECs depends on the activation of AMPK signal pathway. Conclusion This study demonstrates that CLEC3B acts as a novel independent prognostic factor, and CLEC3B in exosomes might be a potential therapeutic target for hepatocellular carcinoma. Graphical abstract
    Keywords CLEC3B ; Exosomes ; Hepatocellular carcinoma ; Endothelial cells ; VEGF ; AMPK ; Medicine ; R ; Cytology ; QH573-671
    Subject code 616
    Language English
    Publishing date 2019-09-01T00:00:00Z
    Publisher BMC
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article: Impact of tryptophan on the formation of TCNM in the process of UV/chlorine disinfection

    Deng, Lin / Longjia Wen / Rajendra Prasad Singh / Wenjuan Dai

    Environmental science and pollution research international. 2018 Aug., v. 25, no. 23

    2018  

    Abstract: Low-pressure (LP) UV treatment after chlorine disinfection was associated with enhanced formation of trichloronitromethane (TCNM), a halonitromethane disinfection by-product (DBP), due to the chlorination of tryptophan. Evidence was found that the ... ...

    Abstract Low-pressure (LP) UV treatment after chlorine disinfection was associated with enhanced formation of trichloronitromethane (TCNM), a halonitromethane disinfection by-product (DBP), due to the chlorination of tryptophan. Evidence was found that the concentration of TCNM from tryptophan increased quickly to the maximum for the first instance. Moreover, the increase of TCNM under UV exceeded 10 times than under dark. Then, it was found to have an obvious decrease in the formation of TCNM, even finally hardly disappear. In order to elucidate reasons for this phenomenon, the effects of light intensity, initial tryptophan concentration, free chlorine concentration, pH, and tert-butanol (TBA) on the formation of TCNM were investigated under UV/chlorine treatment. Finally, the effects of tryptophan on the formation of TCNM and the direct photodegradation of TCNM under LP UV irradiation were studied for analyzing the possible pathways of TCNM formation from amino acid. Since amino acids are very common in water sources, further research into chemical oxidation of these species by LP UV and chlorine is recommended. It can help us to find the precursors of TCNM formation and reduce the risk of TCNM formation for drinking water and wastewater utilities.
    Keywords byproducts ; chlorination ; chlorine ; chloropicrin ; disinfection ; drinking water ; oxidation ; pH ; photolysis ; risk reduction ; tryptophan ; ultraviolet radiation ; wastewater
    Language English
    Dates of publication 2018-08
    Size p. 23227-23235.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    ZDB-ID 1178791-0
    ISSN 1614-7499 ; 0944-1344
    ISSN (online) 1614-7499
    ISSN 0944-1344
    DOI 10.1007/s11356-018-2397-0
    Database NAL-Catalogue (AGRICOLA)

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  4. Article: Drug–drug co-crystallization presents a new opportunity for the development of stable vitamins

    Wang, Jian-Rong / Qihui Yu / Wenjuan Dai / Xuefeng Mei

    Chemical communications. 2016 Feb. 18, v. 52, no. 17

    2016  

    Abstract: Drug–drug co-crystallization could realize combination drugs at a molecular level. Two polymorphic co-crystals between VD₂ and VD₃ were successfully designed and synthesized. These enantiotropic polymorphs exhibit significantly different physicochemical ... ...

    Abstract Drug–drug co-crystallization could realize combination drugs at a molecular level. Two polymorphic co-crystals between VD₂ and VD₃ were successfully designed and synthesized. These enantiotropic polymorphs exhibit significantly different physicochemical stabilities.
    Keywords chemical compounds ; chemical reactions ; vitamins
    Language English
    Dates of publication 2016-0218
    Size p. 3572-3575.
    Publishing place The Royal Society of Chemistry
    Document type Article
    ZDB-ID 1472881-3
    ISSN 1364-548X ; 1359-7345 ; 0009-241X
    ISSN (online) 1364-548X
    ISSN 1359-7345 ; 0009-241X
    DOI 10.1039/c5cc10297a
    Database NAL-Catalogue (AGRICOLA)

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