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  1. Article: Dynamic translocation of stilbene synthase VpSTS29 from a Chinese wild Vitis species upon UV irradiation

    Ma, Fuli / Lei Wang / Wenkong Yao / Yuejin Wang

    Phytochemistry. 2019 Mar., v. 159

    2019  

    Abstract: Stilbene phytoalexins derived from grapevine can be rapidly accumulated when exposed to an artificial UV-C treatment. However, the underlying mechanisms involved in this accumulation and translocation are unclear. Here, we describe an investigation of ... ...

    Abstract Stilbene phytoalexins derived from grapevine can be rapidly accumulated when exposed to an artificial UV-C treatment. However, the underlying mechanisms involved in this accumulation and translocation are unclear. Here, we describe an investigation of the influence of UV-C treatment on the dynamic subcellular distribution of a member of a stilbene synthase family VpSTS29 derived from Chinese wild Vitis pseudoreticulata W.T. Wang when over-expressed in V. vinifera L. cv. Thompson Seedless. Our results show that VpSTS29-GFP was accumulated at a relatively high level in roots and mature leaves of transgenic grape lines, and was predominantly distributed in the cytoplasm. When exposed to UV-C irradiation, VpSTS29 displayed UV-induced feature coupled with the accumulation of stilbene compounds. Notably, VpSTS29-GFP can be translocated from the cytoplasm into chloroplasts upon UV-irradiation. Leaves from the two VpSTS29-GFP-expressing lines displayed more serious UV damage, showing withering and marginal scorching phenotype, and decreased content of H2O2, compared to the untransformed plant. Also, overexpression of VpSTS29 altered the expression of genes related to redox regulation, stilbene biosynthesis and light stimulus. Co-expression of VpSTS29-GFP with Glycolate oxidase 1 (myc-VpGLO1) confirmed the ability of stilbenes to decrease the content of H2O2 in Arabidopsis mesophyll protoplasts. These results provide new insight into the biological functions and properties of stilbene synthase and its product in response to environmental stimulus.
    Keywords (S)-2-hydroxy-acid oxidase ; Arabidopsis ; biosynthesis ; chloroplasts ; gene overexpression ; grapes ; hydrogen peroxide ; irradiation ; leaves ; mesophyll ; phenotype ; phytoalexins ; protoplasts ; roots ; stilbenes ; transgenic plants ; trihydroxystilbene synthase ; ultraviolet radiation ; Vitis vinifera
    Language English
    Dates of publication 2019-03
    Size p. 137-147.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 208884-8
    ISSN 1873-3700 ; 0031-9422
    ISSN (online) 1873-3700
    ISSN 0031-9422
    DOI 10.1016/j.phytochem.2018.12.019
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 1586: Show issues
    Z 8.5: Show issues

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  2. Article: Overexpression of VpEIFP1, a novel F-box/Kelch-repeat protein from wild Chinese Vitis pseudoreticulata, confers higher tolerance to powdery mildew by inducing thioredoxin z proteolysis

    Wang, Jie / Changyue Jiang / Chaohong Zhang / Chen Wang / Fuli Ma / Jianxia Zhang / Lei Wang / Rui Bao / Weihuo Tong / Wenkong Yao / Xiping Wang / Yan Xu / Yazhou Yang / Yuejin Wang

    Plant science. 2017 Oct., v. 263

    2017  

    Abstract: An F-box protein (VpEIFP1) induced by Erysiphe necator was isolated from Vitis pseudoreticulata, a wild Chinese grapevine species naturally resistant to powdery mildew (PM). It contains an F-box domain and two Kelch-repeat motifs. Expression profiles ... ...

    Abstract An F-box protein (VpEIFP1) induced by Erysiphe necator was isolated from Vitis pseudoreticulata, a wild Chinese grapevine species naturally resistant to powdery mildew (PM). It contains an F-box domain and two Kelch-repeat motifs. Expression profiles indicate the VpEIFP1 is strongly induced at both transcriptional and translational levels by PM infection. A subcellular localisation assay showed that VpEIFP1 is predominantly located in the nucleus and cytoplasm. Overexpression of VpEIFP1 accelerated the accumulation of hydrogen peroxide (H2O2) and up-regulated the expressions of ICS2, NPR1 and PR1 involved in defence responses, resulting in suppression of PM germination and growth. As an F-box protein, VpEIFP1 interacts with thioredoxin z (VpTrxz) in the yeast-two-hybrid (Y2H) assay and in the bimolecular fluorescence complementation (BiFC) assay. Decreased amounts of VpTrxz protein in transgenic grapevine leaves overexpressing VpEIFP1 were restored by proteasome inhibitor MG132, implying that VpEIFP1 mediated VpTrxz for degradation through the SCFVpEIFP1 (Skp1-Cullin-F-box) E3 ubiquitin ligase complex. The RNA interference line of VpTrxz showed increased H2O2 accumulation following PM inoculation. We propose VpEIFP1 positively modulates the grapevine defence response to PM by inducing the degradation of VpTrxz via the ubiquitin/26S proteasome system.
    Keywords cytoplasm ; disease resistance ; F-box proteins ; fluorescence ; gene overexpression ; genetically modified organisms ; germination ; hydrogen peroxide ; Kelch repeats ; leaves ; powdery mildew ; proteasome endopeptidase complex ; proteasome inhibitors ; proteolysis ; RNA interference ; thioredoxins ; transcription (genetics) ; translation (genetics) ; ubiquitin-protein ligase ; Uncinula necator ; Vitis
    Language English
    Dates of publication 2017-10
    Size p. 142-155.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 742010-9
    ISSN 1873-2259 ; 0168-9452
    ISSN (online) 1873-2259
    ISSN 0168-9452
    DOI 10.1016/j.plantsci.2017.07.004
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 77/717: Show issues

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