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  1. Article ; Online: Environmental Stability of Seed Carbohydrate Profiles in Soybeans Containing Different Alleles of the Raffinose Synthase 2 (RS2) Gene.

    Bilyeu, Kristin D / Wiebold, William J

    Journal of agricultural and food chemistry

    2016  Volume 64, Issue 5, Page(s) 1071–1078

    Abstract: Soybean [Glycine max (L.) Merr.] is important for the high protein meal used for livestock feed formulations. Carbohydrates contribute positively or negatively to the potential metabolizable energy in soybean meal. The positive carbohydrate present in ... ...

    Abstract Soybean [Glycine max (L.) Merr.] is important for the high protein meal used for livestock feed formulations. Carbohydrates contribute positively or negatively to the potential metabolizable energy in soybean meal. The positive carbohydrate present in soybean meal consists primarily of sucrose, whereas the negative carbohydrate components are the raffinose family of oligosaccharides (RFOs), raffinose and stachyose. Increasing sucrose and decreasing raffinose and stachyose are critical targets to improve soybean. In three recently characterized lines, variant alleles of the soybean raffinose synthase 2 (RS2) gene were associated with increased sucrose and decreased RFOs. The objective of this research was to compare the environmental stability of seed carbohydrates in soybean lines containing wild-type or variant alleles of RS2 utilizing a field location study and a date of planting study. The results define the carbohydrate variation in distinct regional and temporal environments using soybean lines with different alleles of the RS2 gene.
    MeSH term(s) Alleles ; Carbohydrates/chemistry ; Environment ; Galactosyltransferases/genetics ; Galactosyltransferases/metabolism ; Genotype ; Seeds/chemistry ; Seeds/genetics ; Seeds/metabolism ; Soybean Proteins/genetics ; Soybean Proteins/metabolism ; Glycine max/chemistry ; Glycine max/genetics ; Glycine max/metabolism
    Chemical Substances Carbohydrates ; Soybean Proteins ; Galactosyltransferases (EC 2.4.1.-) ; raffinose synthase (EC 2.4.1.82)
    Language English
    Publishing date 2016-01-29
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 241619-0
    ISSN 1520-5118 ; 0021-8561
    ISSN (online) 1520-5118
    ISSN 0021-8561
    DOI 10.1021/acs.jafc.5b04779
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1172: Show issues Location:
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  2. Article: Development of soybean experimental lines with enhanced protein and sulfur amino acid content

    Alaswad, Alaa A / Song, Bo / Oehrle, Nathan W / Wiebold, William J / Mawhinney, Thomas P / Krishnan, Hari B

    Plant science. 2021 July, v. 308

    2021  

    Abstract: Soybean is the preferred protein source for both poultry and swine feed. However, this preferred status is being challenged due to competition from alternative feed ingredients. To overcome this, it becomes necessary for breeders to develop soybean ... ...

    Abstract Soybean is the preferred protein source for both poultry and swine feed. However, this preferred status is being challenged due to competition from alternative feed ingredients. To overcome this, it becomes necessary for breeders to develop soybean cultivars that contain higher protein and better nutritional composition. In this study, we have developed experimental soybean lines that not only contain significantly higher amounts of protein but also improved sulfur amino acid content. This objective was achieved by crossing a O-acetylserine sulfhydrylase (OASS) overexpressing transgenic soybean line with elevated levels of sulfur amino acid content (CS) with a high protein Korean soybean cultivar (Lee 5). Introgression of high protein and overexpression of OASS was monitored in the experimental lines at each successive generation (F2-F6) by measuring protein content and OASS activity. The average protein content of transgenic CS and Lee 5 seeds were 34.8 % and 44.7 %, while in the experimental soybean lines the protein content ranged from 41.3 %–47.7 %, respectively. HPLC and inductively coupled plasma-mass spectrometry analyses revealed that all the experimental lines developed in this study contained significantly higher amounts of sulfur containing amino acids and elemental sulfur in the seeds. The sulfur amino acid (cysteine + methionine) content of the experimental lines ranged from 1.1 % to 1.26 % while the parents Lee 5 and CS had 0.79 % and 1.1 %, respectively. SDS-PAGE and western blot analysis demonstrated that the accumulation of Bowman-Birk protease inhibitor and lunasin, two sulfur amino acid rich peptides, were elevated in experimental soybean lines. High-resolution 2D-gel electrophoresis and Delta2D gel analysis validated that an overall increase in the different subunits of 7S β-conglycinin and 11S glycinin were mainly responsible for the observed increase in the total amount of protein in experimental lines.
    Keywords Bowman-Birk inhibitor ; Western blotting ; amino acid composition ; cultivars ; cysteine ; cysteine synthase ; gels ; glycinin ; introgression ; methionine ; nutrient content ; peptides ; polyacrylamide gel electrophoresis ; poultry ; protein content ; protein sources ; soybeans ; sulfur ; swine
    Language English
    Dates of publication 2021-07
    Publishing place Elsevier B.V.
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 742010-9
    ISSN 1873-2259 ; 0168-9452
    ISSN (online) 1873-2259
    ISSN 0168-9452
    DOI 10.1016/j.plantsci.2021.110912
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    Z 77/717: Show issues

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  3. Article ; Online: Development of soybean experimental lines with enhanced protein and sulfur amino acid content.

    Alaswad, Alaa A / Song, Bo / Oehrle, Nathan W / Wiebold, William J / Mawhinney, Thomas P / Krishnan, Hari B

    Plant science : an international journal of experimental plant biology

    2021  Volume 308, Page(s) 110912

    Abstract: Soybean is the preferred protein source for both poultry and swine feed. However, this preferred status is being challenged due to competition from alternative feed ingredients. To overcome this, it becomes necessary for breeders to develop soybean ... ...

    Abstract Soybean is the preferred protein source for both poultry and swine feed. However, this preferred status is being challenged due to competition from alternative feed ingredients. To overcome this, it becomes necessary for breeders to develop soybean cultivars that contain higher protein and better nutritional composition. In this study, we have developed experimental soybean lines that not only contain significantly higher amounts of protein but also improved sulfur amino acid content. This objective was achieved by crossing a O-acetylserine sulfhydrylase (OASS) overexpressing transgenic soybean line with elevated levels of sulfur amino acid content (CS) with a high protein Korean soybean cultivar (Lee 5). Introgression of high protein and overexpression of OASS was monitored in the experimental lines at each successive generation (F2-F6) by measuring protein content and OASS activity. The average protein content of transgenic CS and Lee 5 seeds were 34.8 % and 44.7 %, while in the experimental soybean lines the protein content ranged from 41.3 %-47.7 %, respectively. HPLC and inductively coupled plasma-mass spectrometry analyses revealed that all the experimental lines developed in this study contained significantly higher amounts of sulfur containing amino acids and elemental sulfur in the seeds. The sulfur amino acid (cysteine + methionine) content of the experimental lines ranged from 1.1 % to 1.26 % while the parents Lee 5 and CS had 0.79 % and 1.1 %, respectively. SDS-PAGE and western blot analysis demonstrated that the accumulation of Bowman-Birk protease inhibitor and lunasin, two sulfur amino acid rich peptides, were elevated in experimental soybean lines. High-resolution 2D-gel electrophoresis and Delta2D gel analysis validated that an overall increase in the different subunits of 7S β-conglycinin and 11S glycinin were mainly responsible for the observed increase in the total amount of protein in experimental lines.
    MeSH term(s) Amino Acids, Sulfur/analysis ; Plant Breeding ; Plant Proteins/analysis ; Plants, Genetically Modified ; Glycine max/chemistry ; Glycine max/genetics
    Chemical Substances Amino Acids, Sulfur ; Plant Proteins
    Language English
    Publishing date 2021-04-18
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 742010-9
    ISSN 1873-2259 ; 0168-9452
    ISSN (online) 1873-2259
    ISSN 0168-9452
    DOI 10.1016/j.plantsci.2021.110912
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 77/717: Show issues

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  4. Book: Grain sorghum

    Wiebold, William J

    2002 Missouri crop performance

    (Special report ; 544)

    2002  

    Author's details Wiebold ... [et al.]
    Series title Special report ; 544
    Language English
    Size 17 p. :, map ;, 28 cm.
    Publisher Agricultural Experiment Station, College of Agriculture Food and Natural Resources, University of Missouri--Columbia
    Publishing place Columbia, Mo
    Document type Book
    Note Cover title. ; Chiefly tables. ; "November 2002."
    Database NAL-Catalogue (AGRICOLA)

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  5. Book: Corn

    Wiebold, William J

    2002 Missouri crop performance

    (Special report / Agricultural Experiment Station, College of Agriculture Food and Natural Resources, University of Missouri-Columbia ; 543)

    2002  

    Title variant Missouri 2002 corn performance trials
    Author's details Wiebold ... [et al.]
    Series title Special report / Agricultural Experiment Station, College of Agriculture Food and Natural Resources, University of Missouri-Columbia ; 543
    Keywords plants ; plant cultural practices ; field crops ; crop production ; agronomy
    Language English
    Size 81 p. :, map ;, 28 cm.
    Publisher Agricultural Experiment Station, College of Agriculture Food and Natural Resources, University of Missouri-Columbia
    Publishing place Columbia, Mo.
    Document type Book
    Note Cover title. ; "October 2002." ; Chiefly tables.
    Database NAL-Catalogue (AGRICOLA)

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  6. Book: Soybean

    Wiebold, William J

    2002 Missouri crop performance

    (Special report / Agricultural Experiment Station, College of Agriculture Food and Natural Resources, University of Missouri-Columbia ; 546)

    2002  

    Title variant Missouri 2002 soybean performance trials
    Author's details Wiebold ... [et al.]
    Series title Special report / Agricultural Experiment Station, College of Agriculture Food and Natural Resources, University of Missouri-Columbia ; 546
    Keywords plants ; plant cultural practices ; field crops ; crop production ; agronomy
    Language English
    Size 94 p. :, map ;, 28 cm.
    Publisher Agricultural Experiment Station, College of Agriculture Food and Natural Resources, University of Missouri-Columbia
    Publishing place Columbia, Mo.
    Document type Book
    Note Cover title. ; "December 2002." ; Chiefly tables.
    Database NAL-Catalogue (AGRICOLA)

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  7. Book: Soybean

    Wiebold, William J

    2000 Missouri crop performance

    (Special report / Agricultural Experiment Station, College of Agriculture Food and Natural Resources, University of Missouri-Columbia ; 534)

    2000  

    Title variant Missouri crop performance 2000, soybean
    Author's details Wiebold ... [et al.]
    Series title Special report / Agricultural Experiment Station, College of Agriculture Food and Natural Resources, University of Missouri-Columbia ; 534
    Language English
    Size 132 p. :, ill., maps ;, 28 cm.
    Publisher Agricultural Experiment Station, College of Agriculture, Food, and Natural Resources, University of Missouri-Columbia
    Publishing place Columbia, Mo.
    Document type Book
    Note Cover title. ; Chiefly tables. ; "December 2000."
    Database NAL-Catalogue (AGRICOLA)

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  8. Article: Regional analysis of planting date and cultivar maturity recommendations that improve soybean oil yield and meal protein concentration.

    Salmerón, Montserrat / Bourland, Fred M / Buehring, Normie W / Earnest, Larry / Fritschi, Felix B / Gbur, Edward E / Golden, Bobby R / Hathcoat, Daniel / Lofton, Josh / McClure, Angela Thompson / Miller, Travis D / Neely, Clark / Shannon, Grover / Udeigwe, Theophilus K / Verbree, David A / Vories, Earl D / Wiebold, William J / Purcell, Larry C

    Frontiers in plant science

    2022  Volume 13, Page(s) 954111

    Abstract: Planting date and cultivar maturity group (MG) are major management factors affecting soybean [ ...

    Abstract Planting date and cultivar maturity group (MG) are major management factors affecting soybean [
    Language English
    Publishing date 2022-10-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2022.954111
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Removing Corn Stover Has Little Effect on Soybean Performance in a Corn–Soybean Rotation

    Nelson, Kellar E / Boardman-Smith, Deanna S / Wiebold, William J

    Crop management. 2013 Dec., v. 12, no. 1

    2013  

    Abstract: Corn (Zea mays) stover is an attractive choice for cellulosic ethanol production because it is readily available and easily obtainable. Understanding how corn stover removal affects soybean (Glycine max) emergence and yield is essential to developing ... ...

    Abstract Corn (Zea mays) stover is an attractive choice for cellulosic ethanol production because it is readily available and easily obtainable. Understanding how corn stover removal affects soybean (Glycine max) emergence and yield is essential to developing appropriate management recommendations for both crops in a corn–soybean rotation. The objectives of this study were to determine (i) the effects of corn stover removal on soybean emergence and yield and (ii) if cover crops ameliorate any of the possible negative effects to soybean from corn stover removal. Removing stover allowed soil temperature to increase, slightly, but cover crops had little or no effect. Stover removal and cover crops had no effect on three soybean emergence parameters except stover removal resulted in a more nearly uniform stand in 1 yr. Soybean stand density increased with stover removal. Soybean grain yield was not affected by either stover removal or the use of cover crops. Removing corn stover may have long term effects on important soil characteristics and these may impact future grain-crop productivity.
    Keywords Glycine max ; Zea mays ; bioethanol ; corn ; corn stover ; cover crops ; crop rotation ; ethanol production ; grain yield ; long term effects ; soil temperature ; soybeans ; stand density
    Language English
    Dates of publication 2013-12
    Publishing place Plant Management Network
    Document type Article
    Note epub
    ISSN 1543-7833
    DOI 10.1094/CM-2012-0177-RS
    Database NAL-Catalogue (AGRICOLA)

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  10. Article: Growing Location has a Pronounced Effect on the Accumulation of Cancer Chemopreventive Agent Bowman-Birk Inhibitor in Soybean Seeds

    Krishnan, Hari B / Jang, Sungchan / Baxter, Ivan / Wiebold, William J

    Crop science. 2012 July, v. 52, no. 4

    2012  

    Abstract: Soybean [ (L.) Merr.] contains several health promoting compounds including phytosterols, isoflavones, phytic acid, and protease inhibitors. The two abundant protease inhibitors of soybean seeds are the Kunitz trypsin inhibitor and the Bowman-Birk ... ...

    Abstract Soybean [ (L.) Merr.] contains several health promoting compounds including phytosterols, isoflavones, phytic acid, and protease inhibitors. The two abundant protease inhibitors of soybean seeds are the Kunitz trypsin inhibitor and the Bowman-Birk inhibitor (BBI). Bowman-Birk inhibitor has been touted as a potential cancer chemopreventive agent for humans. Little information is available on the effect of growing location on the accumulation of this cancer chemopreventive agent. In this study we have examined the protein profile of eight soybean varieties that were grown in three Missouri locations in 2009 and 2010. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis demonstrated that soybean varieties that were grown in Grand Pass contained elevated levels of the subunit of -conglycinin and reduced amounts of BBI. This observation was further confirmed by Western blot analysis. This difference in the levels of BBI was also reflected in the chymotrypsin inhibitor activity. Growing location also influenced the overall S content of soybean seeds as evidenced by inductively coupled plasma mass spectrometry analysis. Seeds grown in Grand Pass had lower amounts of total S content in both 2009 and 2010. Our results demonstrate that growing location has a profound effect on the accumulation of BBI and it is possible to modulate the concentration of this cancer chemopreventive agent by simple changes in agronomic practices.
    Keywords Bowman-Birk inhibitor ; Glycine max ; Western blotting ; antineoplastic agents ; beta-conglycinin ; bioaccumulation ; chymotrypsin ; humans ; isoflavones ; mass spectrometry ; phytic acid ; plant cultural practices ; polyacrylamide gel electrophoresis ; seeds ; sodium ; soybeans ; Missouri
    Language English
    Dates of publication 2012-07
    Size p. 1786-1794.
    Publishing place The Crop Science Society of America, Inc.
    Document type Article
    Note epub
    ZDB-ID 410209-5
    ISSN 0011-183X
    ISSN 0011-183X
    DOI 10.2135/cropsci2011.11.0593
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    Z 1450: Show issues
    Zs Lan CRPSAY: Show issues

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