LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 41

Search options

  1. Article: Using the Traditional Ex Vivo Whole Blood Model to Discriminate Bacteria by Their Inducible Host Responses.

    Chick, Heather M / Rees, Megan E / Lewis, Matthew L / Williams, Lisa K / Bodger, Owen / Harris, Llinos G / Rushton, Steven / Wilkinson, Thomas S

    Biomedicines

    2024  Volume 12, Issue 4

    Abstract: Whole blood models are rapid and versatile for determining immune responses to inflammatory and infectious stimuli, but they have not been used for bacterial discrimination. ...

    Abstract Whole blood models are rapid and versatile for determining immune responses to inflammatory and infectious stimuli, but they have not been used for bacterial discrimination.
    Language English
    Publishing date 2024-03-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720867-9
    ISSN 2227-9059
    ISSN 2227-9059
    DOI 10.3390/biomedicines12040724
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article ; Online: Reimagining laboratory-based immunology education in the time of COVID-19.

    Wilkinson, Thomas S / Nibbs, Rob / Francis, Nigel J

    Immunology

    2021  Volume 163, Issue 4, Page(s) 431–435

    Abstract: The pandemic has brought challenges to teaching lab and research skills. Here Nigel Francis and colleagues explore the diverse approaches taken to replace lab-based immunology teaching, explain how networks of educators have driven this innovation and ... ...

    Abstract The pandemic has brought challenges to teaching lab and research skills. Here Nigel Francis and colleagues explore the diverse approaches taken to replace lab-based immunology teaching, explain how networks of educators have driven this innovation and discuss the importance of retaining best practice into the future.
    MeSH term(s) Allergy and Immunology/education ; Biomedical Research/education ; COVID-19 ; Computer-Assisted Instruction ; Curriculum ; Education, Distance ; Humans ; Laboratories ; Students ; Teaching
    Language English
    Publishing date 2021-06-06
    Publishing country England
    Document type Journal Article
    ZDB-ID 80124-0
    ISSN 1365-2567 ; 0019-2805 ; 0953-4954
    ISSN (online) 1365-2567
    ISSN 0019-2805 ; 0953-4954
    DOI 10.1111/imm.13369
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.181: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article: An In Vitro Model to Assess Early Immune Markers Following Co-Exposure of Epithelial Cells to Carbon Black (Nano)Particles in the Presence of

    Brown, Scott / Evans, Stephen J / Burgum, Michael J / Meldrum, Kirsty / Herridge, Jack / Akinbola, Blessing / Harris, Llinos G / Jenkins, Rowena / Doak, Shareen H / Clift, Martin J D / Wilkinson, Thomas S

    Biomedicines

    2024  Volume 12, Issue 1

    Abstract: The exposure of human lung and skin to carbon black (CB) is continuous due to its widespread applications. Current toxicological testing uses 'healthy' cellular systems; however, questions remain whether this mimics the everyday stresses that human cells ...

    Abstract The exposure of human lung and skin to carbon black (CB) is continuous due to its widespread applications. Current toxicological testing uses 'healthy' cellular systems; however, questions remain whether this mimics the everyday stresses that human cells are exposed to, including infection.
    Language English
    Publishing date 2024-01-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720867-9
    ISSN 2227-9059
    ISSN 2227-9059
    DOI 10.3390/biomedicines12010128
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article: Temporal Changes in Patient-Matched

    Harris, Llinos G / Bodger, Owen / Post, Virginia / Mack, Dietrich / Morgenstern, Mario / Rohde, Holger / Moriarty, T Fintan / Wilkinson, Thomas S

    Microorganisms

    2020  Volume 8, Issue 10

    Abstract: Staphylococcus ... ...

    Abstract Staphylococcus epidermidis
    Language English
    Publishing date 2020-09-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms8101508
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article ; Online: Contrasting effects of linezolid on healthy and dysfunctional human neutrophils: reducing C5a-induced injury.

    Evans, Stephen J / Roberts, Aled E L / Morris, Andrew Conway / Simpson, A John / Harris, Llinos G / Mack, Dietrich / Jenkins, Rowena E / Wilkinson, Thomas S

    Scientific reports

    2020  Volume 10, Issue 1, Page(s) 16377

    Abstract: Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of ventilator-associated pneumonia (VAP). Patients with VAP have poorly functioning neutrophils, related to increased levels of the complement fragment C5a. The antibiotic linezolid ...

    Abstract Methicillin-resistant Staphylococcus aureus (MRSA) is an important cause of ventilator-associated pneumonia (VAP). Patients with VAP have poorly functioning neutrophils, related to increased levels of the complement fragment C5a. The antibiotic linezolid has been useful in controlling MRSA-related VAP infections; however clinical benefit does not always correlate with antimicrobial effect, suggesting the possibility of immunomodulatory properties. Here the effects of linezolid on healthy and dysfunctional neutrophils (modelled by C5a-induced injury) was investigated. Functional assays (killing, phagocytosis, transmigration, and respiratory burst) were used to assess the effects of pre-, co- and post-incubating linezolid (0.4-40 mg/L) with healthy neutrophils relative to those with C5a-induced injury. C5a decreased neutrophil killing, and phagocytosis of MRSA. Furthermore, C5a significantly decreased neutrophil transmigration to IL-8, but did not affect respiratory burst. Co-incubation of linezolid significantly improved killing of MRSA by dysfunctional neutrophils, which was supported by concomitant increases in phagocytosis. Conversely linezolid impaired killing responses in healthy neutrophils. Pre- or post-incubation of linezolid prior or following C5a induced injury had no effect on neutrophil function. This study suggests that linezolid has immunomodulatory properties that protect human neutrophils from injury and provides insight into its mode of action beyond a basic antibiotic.
    MeSH term(s) Anti-Bacterial Agents/therapeutic use ; Complement C5a/metabolism ; Humans ; Linezolid/therapeutic use ; Methicillin-Resistant Staphylococcus aureus/drug effects ; Neutrophils/drug effects ; Phagocytosis/drug effects ; Pneumonia, Ventilator-Associated/drug therapy ; Pneumonia, Ventilator-Associated/metabolism ; Pneumonia, Ventilator-Associated/microbiology ; Respiratory Burst/drug effects
    Chemical Substances Anti-Bacterial Agents ; Complement C5a (80295-54-1) ; Linezolid (ISQ9I6J12J)
    Language English
    Publishing date 2020-10-02
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-020-72454-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article ; Online: Antibacterial and Antivirulence Activity of Manuka Honey against Genetically Diverse Staphylococcus pseudintermedius Strains.

    Brown, Helen L / Metters, Georgie / Hitchings, Matthew D / Wilkinson, Thomas S / Sousa, Luis / Cooper, Jenna / Dance, Harry / Atterbury, Robert J / Jenkins, Rowena

    Applied and environmental microbiology

    2020  Volume 86, Issue 20

    Abstract: Staphylococcus ... ...

    Abstract Staphylococcus pseudintermedius
    MeSH term(s) Anti-Bacterial Agents/analysis ; Anti-Bacterial Agents/pharmacology ; Honey/analysis ; Staphylococcus/drug effects ; Staphylococcus/genetics ; Staphylococcus/pathogenicity ; Staphylococcus/physiology ; Virulence/drug effects
    Chemical Substances Anti-Bacterial Agents
    Language English
    Publishing date 2020-10-01
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 223011-2
    ISSN 1098-5336 ; 0099-2240
    ISSN (online) 1098-5336
    ISSN 0099-2240
    DOI 10.1128/AEM.01768-20
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 201: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  7. Article: Temporal Changes in Patient-Matched Staphylococcus epidermidis Isolates from Infections: towards Defining a ‘True’ Persistent Infection

    Harris, Llinos G / Bodger, Owen / Post, Virginia / Mack, Dietrich / Morgenstern, Mario / Rohde, Holger / Moriarty, T. Fintan / Wilkinson, Thomas S

    Microorganisms. 2020 Sept. 30, v. 8, no. 10

    2020  

    Abstract: Staphylococcus epidermidis is found naturally on the skin but is a common cause of persistent orthopaedic device-related infections (ODRIs). This study used a pan-genome and gene-by-gene approach to analyse the clonality of whole genome sequences (WGS) ... ...

    Abstract Staphylococcus epidermidis is found naturally on the skin but is a common cause of persistent orthopaedic device-related infections (ODRIs). This study used a pan-genome and gene-by-gene approach to analyse the clonality of whole genome sequences (WGS) of 115 S. epidermidis isolates from 55 patients with persistent ODRIs. Analysis of the 522 gene core genome revealed that the isolates clustered into three clades, and MLST analysis showed that 83% of the isolates belonged to clonal complex 2 (CC2). Analysis also found 13 isolate pairs had different MLST types and less than 70% similarity within the genes; hence, these were defined as re-infection by a different S. epidermidis strain. Comparison of allelic diversity in the remaining 102 isolates (49 patients) revealed that 6 patients had microevolved infections (>7 allele differences), and only 37 patients (77 isolates) had a ‘true’ persistent infection. Analysis of the core genomes of isolate pairs from 37 patients found 110/841 genes had variations; mainly in metabolism associated genes. The accessory genome consisted of 2936 genes; with an average size of 1515 genes. To conclude, this study demonstrates the advantage of using WGS for identifying the accuracy of a persistent infection diagnosis. Hence, persistent infections can be defined as ‘true’ persistent infections if the core genome of paired isolates has ≤7 allele differences; microevolved persistent infection if the paired isolates have >7 allele differences but same MLST type; and polyclonal if they are the same species but a different MLST type.
    Keywords Staphylococcus epidermidis ; accuracy ; alleles ; allelic variation ; chronic diseases ; metabolism ; microorganisms ; orthopedics ; patients ; temporal variation
    Language English
    Dates of publication 2020-0930
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    Note NAL-light
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms8101508
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  8. Article: The Bacterial Species

    John, Daniel A / Williams, Lisa K / Kanamarlapudi, Venkateswarlu / Humphrey, Thomas J / Wilkinson, Thomas S

    Frontiers in microbiology

    2017  Volume 8, Page(s) 1840

    Abstract: ... ...

    Abstract Campylobacter
    Language English
    Publishing date 2017-09-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2017.01840
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  9. Article ; Online: Limitations in the use of PSMγ, agr, RNAIII, and biofilm formation as biomarkers to define invasive Staphylococcus epidermidis from chronic biomedical device-associated infections.

    Harris, Llinos G / Dudley, Ed / Rohde, Holger / Frommelt, Lars / Siemssen, Nicolaus / Wilkinson, Thomas S / Mack, Dietrich

    International journal of medical microbiology : IJMM

    2017  Volume 307, Issue 7, Page(s) 382–387

    Abstract: Staphylococcus epidermidis is a common cause of biomedical device-associated infections. Agr is the major quorum sensing system in staphylococci and regulates virulence factors. Four agr-specificity groups exist in S. epidermidis, and chronic S. ... ...

    Abstract Staphylococcus epidermidis is a common cause of biomedical device-associated infections. Agr is the major quorum sensing system in staphylococci and regulates virulence factors. Four agr-specificity groups exist in S. epidermidis, and chronic S. epidermidis infections are hypothesised to select for agr-negative phenotypes. Therefore, we investigated S. epidermidis strains from prosthetic joint- and catheter-associated infections to establish i) whether an infection selects for an agr-negative phenotype; ii) the importance of PSMγ and iii) if the agr-specificity group is infection dependent. S. epidermidis nasal isolates from healthy volunteers were used as controls. The distribution of agr-specificity groups was significantly different between infection and control episodes, but did not distinguish between the infection types. PSMγ secretion was used to determine agr-activity and HPLC analysis showed that 44% of prosthetic and 32% of catheter-associated episodes produced no PSMγ in comparison to 8% of the control strains. However, PSMγ expression did not always correlate with RNAIII up-regulation, indicating that PSMγ synthesis is likely influenced by additional post-transcriptional control. The data suggests chronic S. epidermidis infections favour agr-specificity group 1 but the results suggest that they do not select for an agr-negative phenotype. Further studies are required to explore the mechanisms underlying the selection and survival of these S. epidermidis phenotypes isolated from biomedical device-associated infections.
    Language English
    Publishing date 2017-10
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2006518-8
    ISSN 1618-0607 ; 1438-4221
    ISSN (online) 1618-0607
    ISSN 1438-4221
    DOI 10.1016/j.ijmm.2017.08.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 5333: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  10. Article ; Online: In vitro detection of in vitro secondary mechanisms of genotoxicity induced by engineered nanomaterials.

    Evans, Stephen J / Clift, Martin J D / Singh, Neenu / Wills, John W / Hondow, Nicole / Wilkinson, Thomas S / Burgum, Michael J / Brown, Andy P / Jenkins, Gareth J / Doak, Shareen H

    Particle and fibre toxicology

    2019  Volume 16, Issue 1, Page(s) 8

    Abstract: Background: It is well established that toxicological evaluation of engineered nanomaterials (NMs) is vital to ensure the health and safety of those exposed to them. Further, there is a distinct need for the development of advanced physiologically ... ...

    Abstract Background: It is well established that toxicological evaluation of engineered nanomaterials (NMs) is vital to ensure the health and safety of those exposed to them. Further, there is a distinct need for the development of advanced physiologically relevant in vitro techniques for NM hazard prediction due to the limited predictive power of current in vitro models and the unsustainability of conducting nano-safety evaluations in vivo. Thus, the purpose of this study was to develop alternative in vitro approaches to assess the potential of NMs to induce genotoxicity by secondary mechanisms.
    Results: This was first undertaken by a conditioned media-based technique, whereby cell culture media was transferred from differentiated THP-1 (dTHP-1) macrophages treated with γ-Fe
    Conclusions: The findings of the present study demonstrate that the approach of using single in vitro cell test systems precludes the ability to consider secondary genotoxic mechanisms. Consequently, the use of multi-cell type models is preferable as they better mimic the in vivo environment and thus offer the potential to enhance understanding and detection of a wider breadth of potential damage induced by NMs.
    MeSH term(s) Bronchi/drug effects ; Bronchi/immunology ; Bronchi/pathology ; Cell Differentiation/drug effects ; Cell Differentiation/genetics ; Cell Survival/drug effects ; Coculture Techniques ; Culture Media, Conditioned ; Cytokines/biosynthesis ; DNA Damage ; Endocytosis/drug effects ; Ferric Compounds/toxicity ; Humans ; In Vitro Techniques ; Macrophages/drug effects ; Macrophages/immunology ; Macrophages/pathology ; Magnetite Nanoparticles/toxicity ; Mutagenicity Tests/methods ; THP-1 Cells
    Chemical Substances Culture Media, Conditioned ; Cytokines ; Ferric Compounds ; Magnetite Nanoparticles ; ferric oxide (1K09F3G675)
    Language English
    Publishing date 2019-02-13
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2170936-1
    ISSN 1743-8977 ; 1743-8977
    ISSN (online) 1743-8977
    ISSN 1743-8977
    DOI 10.1186/s12989-019-0291-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top