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  1. Article: Tracer tests to infer the drainage of the multiple porosity aquifer of Luxembourg Sandstone (Grand-Duchy of Luxembourg): implications for drinking water protection

    Meus, Philippe / Willems, Luc

    Hydrogeology journal. 2021 Feb., v. 29, no. 1

    2021  

    Abstract: The Luxembourg Sandstone is the main strategic aquifer of the Grand-Duchy of Luxembourg, providing one third of the total drinking water resource in the country. In the unconfined part, the aquifer discharges through more than 400 springs, which are used ...

    Title translation Essais de traçage artificiels pour déduire le drainage de l’aquifère à multi-porosité du grès de Luxembourg (Grand-Duché de Luxembourg): conséquences pour la protection de l’eau potable Pruebas de trazadores para inferir el drenaje del acuífero de porosidad múltiple de la arenisca de Luxemburgo (Grand-Duchy de Luxemburgo): consecuencias para la protección del agua potable 示踪试验推断Luxembourg砂岩(Luxembourg大公国)多孔隙含水层的排水情况:对饮用水保护的影响 Testes de traçadores rara inferir a drenagem em aquífero de porosidade múltipla no Arenito Luxemburgo (Grão-Ducado de Luxemburgo): implicações para a proteção da água de beber
    Abstract The Luxembourg Sandstone is the main strategic aquifer of the Grand-Duchy of Luxembourg, providing one third of the total drinking water resource in the country. In the unconfined part, the aquifer discharges through more than 400 springs, which are used for supply. The long-lasting diffuse contamination by pesticides (more recently Metolachor ESA) has been previously simulated assuming the aquifer as an equivalent porous medium, and using environmental tracers and baseflow characteristics. A statistical analysis of the whole data set of artificial tracer tests made for this aquifer (112 injections involving 102 springs) emphasized the role of the main fissures (NE–SW) for preferential groundwater flow and the importance of including them in a conceptual model involving a multiple-porosity aquifer. The results show that these fissures are influencing the whole flow system. The average peak velocity is 16 m/h and the average first arrival (maximum) velocity is 52 m/h (32 m/h for injection-well to spring traces alone), with a recorded maximum velocity of 350 m/h. The threats of infiltrating streams within catchments have also been characterized, suggesting the usefulness of tracer tests for optimizing protective measures. The main benefit of the study is the improvement in the ability to delineate safeguard zones accurately, according to more representative velocities and the strong anisotropy.
    Keywords anisotropy ; aquifers ; base flow ; data collection ; drainage ; groundwater flow ; models ; porosity ; porous media ; sandstone ; statistical analysis ; Luxembourg
    Language English
    Dates of publication 2021-02
    Size p. 461-480.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 1227482-3
    ISSN 0941-2816 ; 1431-2174
    ISSN 0941-2816 ; 1431-2174
    DOI 10.1007/s10040-020-02274-z
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: The 14th International Conference on Human Retrovirology

    Willems Luc

    Retrovirology, Vol 6, Iss 1, p

    HTLV and related retroviruses (July 1–4, 2009; Salvador, Brazil)

    2009  Volume 77

    Abstract: Abstract The "14th International Conference on Human Retrovirology: HTLV and Related Retroviruses" was held in Salvador, Bahia, from July 1 st to July 4 th 2009. The aim of this biennial meeting is to promote discussion and share new findings between ... ...

    Abstract Abstract The "14th International Conference on Human Retrovirology: HTLV and Related Retroviruses" was held in Salvador, Bahia, from July 1 st to July 4 th 2009. The aim of this biennial meeting is to promote discussion and share new findings between researchers and clinicians for the benefit of patients infected by human T-lymphotropic virus (HTLV). HTLV infects approximately 15–20 million individuals worldwide and causes a broad spectrum of diseases including neurodegeneration and leukemia. The scientific program included a breadth of HTLV research topics: epidemiology, host immune response, basic mechanisms of protein function, virology, pathogenesis, clinical aspects and treatment. Exciting new findings were presented in these different fields, and the new advances have led to novel clinical trials. Here, highlights from this conference are summarized.
    Keywords Medicine (General) ; R5-920 ; Medicine ; R ; DOAJ:Medicine (General) ; DOAJ:Health Sciences ; Immunologic diseases. Allergy ; RC581-607
    Subject code 028
    Language English
    Publishing date 2009-08-01T00:00:00Z
    Publisher BioMed Central
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: The 14th International Conference on Human Retrovirology: HTLV and related retroviruses (July 1-4, 2009; Salvador, Brazil).

    Willems, Luc

    Retrovirology

    2009  Volume 6, Page(s) 77

    Abstract: The "14th International Conference on Human Retrovirology: HTLV and Related Retroviruses" was held in Salvador, Bahia, from July 1st to July 4th 2009. The aim of this biennial meeting is to promote discussion and share new findings between researchers ... ...

    Abstract The "14th International Conference on Human Retrovirology: HTLV and Related Retroviruses" was held in Salvador, Bahia, from July 1st to July 4th 2009. The aim of this biennial meeting is to promote discussion and share new findings between researchers and clinicians for the benefit of patients infected by human T-lymphotropic virus (HTLV). HTLV infects approximately 15-20 million individuals worldwide and causes a broad spectrum of diseases including neurodegeneration and leukemia. The scientific program included a breadth of HTLV research topics: epidemiology, host immune response, basic mechanisms of protein function, virology, pathogenesis, clinical aspects and treatment. Exciting new findings were presented in these different fields, and the new advances have led to novel clinical trials. Here, highlights from this conference are summarized.
    MeSH term(s) Biomedical Research/trends ; Brazil ; Deltaretrovirus/pathogenicity ; Deltaretrovirus/physiology ; Deltaretrovirus Infections/epidemiology ; Deltaretrovirus Infections/immunology ; Deltaretrovirus Infections/therapy ; Humans
    Language English
    Publishing date 2009-08-17
    Publishing country England
    Document type Congress ; Editorial ; Research Support, Non-U.S. Gov't
    ISSN 1742-4690
    ISSN (online) 1742-4690
    DOI 10.1186/1742-4690-6-77
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Development of a novel monoclonal antibody-based competitive ELISA for antibody detection against bovine leukemia virus.

    Wang, Jing / Sun, Chao / Hu, Zhe / Wang, Fang / Chang, Jitao / Gao, Ming / Ye, Dandan / Jia, Qi / Zou, Hui / Willems, Luc / Jiang, Zhigang / Yin, Xin

    International journal of biological macromolecules

    2024  Volume 267, Issue Pt 2, Page(s) 131446

    Abstract: Infection with bovine leukemia virus (BLV) leads to enzootic bovine leukosis, the most prevalent neoplastic disease in cattle. Due to the lack of commercially available vaccines, reliable eradication of the disease can be achieved through the testing and ...

    Abstract Infection with bovine leukemia virus (BLV) leads to enzootic bovine leukosis, the most prevalent neoplastic disease in cattle. Due to the lack of commercially available vaccines, reliable eradication of the disease can be achieved through the testing and elimination of BLV antibody-positive animals. In this study, we developed a novel competitive ELISA (cELISA) to detect antibodies against BLV capsid protein p24. Recombinant p24 protein expressed by Escherichia coli, in combination with the monoclonal antibody 2G11 exhibiting exceptional performance, was used for the establishment of the cELISA. Receiver-operating characteristic curve analysis showed that the sensitivity and specificity of the assay were 98.85 % and 98.13 %, respectively. Furthermore, the established cELISA was specific for detecting BLV-specific antibodies, without cross-reactivity to antisera for six other bovine viruses. Significantly, experimental infection of cattle and sheep with BLV revealed that the cELISA accurately monitors seroconversion. In a performance evaluation, the established cELISA displayed a high agreement with Western blotting and the commercial BLV gp51 cELISA kit in the detection of 242 clinical samples, respectively. In conclusion, the novel p24 cELISA exhibited the potential to be a reliable and efficient diagnostic tool for BLV serological detection with a broad application prospect.
    MeSH term(s) Leukemia Virus, Bovine/immunology ; Animals ; Enzyme-Linked Immunosorbent Assay/methods ; Cattle ; Antibodies, Viral/immunology ; Antibodies, Monoclonal/immunology ; Enzootic Bovine Leukosis/diagnosis ; Enzootic Bovine Leukosis/immunology ; Capsid Proteins/immunology ; Sensitivity and Specificity ; Recombinant Proteins/immunology ; ROC Curve
    Chemical Substances Antibodies, Viral ; Antibodies, Monoclonal ; Capsid Proteins ; Recombinant Proteins
    Language English
    Publishing date 2024-04-15
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2024.131446
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Ablation of non-coding RNAs affects bovine leukemia virus B lymphocyte proliferation and abrogates oncogenesis.

    Safari, Roghaiyeh / Jacques, Jean-Rock / Brostaux, Yves / Willems, Luc

    PLoS pathogens

    2020  Volume 16, Issue 5, Page(s) e1008502

    Abstract: Viruses have developed different strategies to escape from immune response. Among these, viral non-coding RNAs are invisible to the immune system and may affect the fate of the host cell. Bovine leukemia virus (BLV) encodes both short (miRNAs) and long ( ... ...

    Abstract Viruses have developed different strategies to escape from immune response. Among these, viral non-coding RNAs are invisible to the immune system and may affect the fate of the host cell. Bovine leukemia virus (BLV) encodes both short (miRNAs) and long (antisense AS1 and AS2) non-coding RNAs. To elucidate the mechanisms associated with BLV non-coding RNAs, we performed phenotypic and transcriptomic analyzes in a reverse genetics system. RNA sequencing of B-lymphocytes revealed that cell proliferation is the most significant mechanism associated with ablation of the viral non-coding RNAs. To assess the biological relevance of this observation, we determined the cell kinetic parameters in vivo using intravenous injection of BrdU and CFSE. Fitting the data to a mathematical model provided the rates of cell proliferation and death. Our data show that deletion of miRNAs correlates with reduced proliferation of the infected cell and lack of pathogenesis.
    MeSH term(s) Animals ; B-Lymphocytes/metabolism ; B-Lymphocytes/pathology ; B-Lymphocytes/virology ; Cattle ; Cell Proliferation ; Cell Transformation, Viral ; Leukemia Virus, Bovine/genetics ; Leukemia Virus, Bovine/metabolism ; Leukemia Virus, Bovine/pathogenicity ; MicroRNAs/genetics ; MicroRNAs/metabolism ; RNA, Antisense/genetics ; RNA, Antisense/metabolism ; RNA, Viral/genetics ; RNA, Viral/metabolism
    Chemical Substances MicroRNAs ; RNA, Antisense ; RNA, Viral
    Language English
    Publishing date 2020-05-14
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2205412-1
    ISSN 1553-7374 ; 1553-7366
    ISSN (online) 1553-7374
    ISSN 1553-7366
    DOI 10.1371/journal.ppat.1008502
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Effects of Naturally Occurring Mutations in Bovine Leukemia Virus 5'-LTR and Tax Gene on Viral Transcriptional Activity.

    Pluta, Aneta / Willems, Luc / Douville, Renée N / Kuźmak, Jacek

    Pathogens (Basel, Switzerland)

    2020  Volume 9, Issue 10

    Abstract: Bovine leukemia virus (BLV) is a deltaretrovirus infecting bovine B cells and causing enzootic bovine leucosis (EBL). The long terminal repeat (LTR) plays an indispensable role in viral gene expression. The BLV Tax protein acts as the main transactivator ...

    Abstract Bovine leukemia virus (BLV) is a deltaretrovirus infecting bovine B cells and causing enzootic bovine leucosis (EBL). The long terminal repeat (LTR) plays an indispensable role in viral gene expression. The BLV Tax protein acts as the main transactivator of LTR-driven transcription of BLV viral genes. The aim of this study was to analyze mutations in the BLV LTR region and
    Language English
    Publishing date 2020-10-13
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens9100836
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Whole genome sequencing of 51 breast cancers reveals that tumors are devoid of bovine leukemia virus DNA.

    Gillet, Nicolas A / Willems, Luc

    Retrovirology

    2016  Volume 13, Issue 1, Page(s) 75

    Abstract: Controversy exists regarding the association of bovine leukemia virus (BLV) and breast cancer. PCR-based experimental evidence indicates that BLV DNA is present in breast tissue and that as many as 37% of cancer cases may be attributable to viral ... ...

    Abstract Controversy exists regarding the association of bovine leukemia virus (BLV) and breast cancer. PCR-based experimental evidence indicates that BLV DNA is present in breast tissue and that as many as 37% of cancer cases may be attributable to viral exposure. Since this association might have major consequences for human health, we evaluated 51 whole genomes of breast cancer samples for the presence of BLV DNA. Among 32 billion sequencing reads retrieved from the NCBI database of genotype and phenotype, none mapped on different strains of the BLV genome. Controls for sequence divergence and proviral loads further validated the approach. This unbiased analysis thus excludes a clonal insertion of BLV in breast tumor cells and strongly argues against an association between BLV and breast cancer.
    MeSH term(s) Animals ; Breast Neoplasms/genetics ; Breast Neoplasms/virology ; Cattle ; DNA, Viral/genetics ; DNA, Viral/isolation & purification ; Databases, Nucleic Acid ; Female ; Genes, Viral ; Genome, Human ; Genome, Viral ; Genotype ; Humans ; Leukemia Virus, Bovine/genetics ; Leukemia Virus, Bovine/isolation & purification ; Sequence Alignment ; Sequence Analysis, DNA
    Chemical Substances DNA, Viral
    Language English
    Publishing date 2016-11-04
    Publishing country England
    Document type Journal Article
    ZDB-ID 2142602-8
    ISSN 1742-4690 ; 1742-4690
    ISSN (online) 1742-4690
    ISSN 1742-4690
    DOI 10.1186/s12977-016-0308-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Analysis of Nucleotide Sequence of Tax, miRNA and LTR of Bovine Leukemia Virus in Cattle with Different Levels of Persistent Lymphocytosis in Russia.

    Pluta, Aneta / Blazhko, Natalia V / Ngirande, Charity / Joris, Thomas / Willems, Luc / Kuźmak, Jacek

    Pathogens (Basel, Switzerland)

    2021  Volume 10, Issue 2

    Abstract: Bovine Leukemia Virus (BLV) is the etiological agent of enzootic bovine leucosis (EBL), a lymphoproliferative disease of the bovine species. In BLV-infected cells, the long terminal repeat (LTR), the viral Tax protein and viral miRNAs promote viral and ... ...

    Abstract Bovine Leukemia Virus (BLV) is the etiological agent of enzootic bovine leucosis (EBL), a lymphoproliferative disease of the bovine species. In BLV-infected cells, the long terminal repeat (LTR), the viral Tax protein and viral miRNAs promote viral and cell proliferation as well as tumorigenesis. Although their respective roles are decisive in BLV biology, little is known about the genetic sequence variation of these parts of the BLV genome and their impact on disease outcome. Therefore, the objective of this study was to assess the relationship between disease progression and sequence variation of the BLV Tax, miRNA and LTR regions in infected animals displaying either low or high levels of persistent lymphocytosis (PL). A statistically significant association was observed between the A(+187)C polymorphism in the downstream activator sequence (DAS) region in LTR (
    Language English
    Publishing date 2021-02-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2695572-6
    ISSN 2076-0817
    ISSN 2076-0817
    DOI 10.3390/pathogens10020246
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Activation of DNA Damage Tolerance Pathways May Improve Immunotherapy of Mesothelioma.

    Brossel, Hélène / Fontaine, Alexis / Hoyos, Clotilde / Jamakhani, Majeed / Willems, Mégane / Hamaidia, Malik / Willems, Luc

    Cancers

    2021  Volume 13, Issue 13

    Abstract: Immunotherapy based on two checkpoint inhibitors (ICI), programmed cell death 1 (PD-1, Nivolumab) and cytotoxic T-lymphocyte 4 (CTLA-4, Ipilimumab), has provided a significant improvement in overall survival for malignant mesothelioma (MM). Despite this ... ...

    Abstract Immunotherapy based on two checkpoint inhibitors (ICI), programmed cell death 1 (PD-1, Nivolumab) and cytotoxic T-lymphocyte 4 (CTLA-4, Ipilimumab), has provided a significant improvement in overall survival for malignant mesothelioma (MM). Despite this major breakthrough, the median overall survival of patients treated with the two ICIs only reached 18.1 months vs. 14 months in standard chemotherapy. With an objective response rate of 40%, only a subset of patients benefits from immunotherapy. A critical step in the success of immunotherapy is the presentation of tumor-derived peptides by the major histocompatibility complex I (MHC-I) of tumor cells. These neoantigens are potentially immunogenic and trigger immune responses orchestrated by cytotoxic cells. In MM, tumor development is nevertheless characterized by a low mutation rate despite major structural chromosomal rearrangements driving oncogenesis (
    Language English
    Publishing date 2021-06-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers13133211
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: APOBEC3 Interference during Replication of Viral Genomes.

    Willems, Luc / Gillet, Nicolas Albert

    Viruses

    2015  Volume 7, Issue 6, Page(s) 2999–3018

    Abstract: Co-evolution of viruses and their hosts has reached a fragile and dynamic equilibrium that allows viral persistence, replication and transmission. In response, infected hosts have developed strategies of defense that counteract the deleterious effects of ...

    Abstract Co-evolution of viruses and their hosts has reached a fragile and dynamic equilibrium that allows viral persistence, replication and transmission. In response, infected hosts have developed strategies of defense that counteract the deleterious effects of viral infections. In particular, single-strand DNA editing by Apolipoprotein B Editing Catalytic subunits proteins 3 (APOBEC3s) is a well-conserved mechanism of mammalian innate immunity that mutates and inactivates viral genomes. In this review, we describe the mechanisms of APOBEC3 editing during viral replication, the viral strategies that prevent APOBEC3 activity and the consequences of APOBEC3 modulation on viral fitness and host genome integrity. Understanding the mechanisms involved reveals new prospects for therapeutic intervention.
    MeSH term(s) Animals ; Cytosine Deaminase/metabolism ; Genetic Variation ; Host-Pathogen Interactions ; Humans ; Immune Evasion ; Mammals ; Mutation ; Virus Replication ; Viruses/immunology
    Chemical Substances Cytosine Deaminase (EC 3.5.4.1)
    Language English
    Publishing date 2015-06-11
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v7062757
    Database MEDical Literature Analysis and Retrieval System OnLINE

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