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Article ; Online: Life sciences today and tomorrow: emerging biotechnologies.

Williamson, E Diane

2017 Volume 37, Issue 5, Page(s) 553–565

Abstract: The purpose of this review is to survey current, emerging and predicted future biotechnologies which are impacting, or are likely to impact in the future on the life sciences, with a projection for the coming 20 years. This review is intended to discuss ... ...

Abstract	The purpose of this review is to survey current, emerging and predicted future biotechnologies which are impacting, or are likely to impact in the future on the life sciences, with a projection for the coming 20 years. This review is intended to discuss current and future technical strategies, and to explore areas of potential growth during the foreseeable future. Information technology approaches have been employed to gather and collate data. Twelve broad categories of biotechnology have been identified which are currently impacting the life sciences and will continue to do so. In some cases, technology areas are being pushed forward by the requirement to deal with contemporary questions such as the need to address the emergence of anti-microbial resistance. In other cases, the biotechnology application is made feasible by advances in allied fields in biophysics (e.g. biosensing) and biochemistry (e.g. bio-imaging). In all cases, the biotechnologies are underpinned by the rapidly advancing fields of information systems, electronic communications and the World Wide Web together with developments in computing power and the capacity to handle extensive biological data. A rationale and narrative is given for the identification of each technology as a growth area. These technologies have been categorized by major applications, and are discussed further. This review highlights: Biotechnology has far-reaching applications which impinge on every aspect of human existence. The applications of biotechnology are currently wide ranging and will become even more diverse in the future. Access to supercomputing facilities and the ability to manipulate large, complex biological datasets, will significantly enhance knowledge and biotechnological development.
MeSH term(s)	Biotechnology
Language	English
Publishing date	2017-08
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	1042364-3
ISSN	1549-7801 ; 0738-8551
ISSN (online)	1549-7801
ISSN	0738-8551
DOI	10.1080/07388551.2016.1201455
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Co-formulation of the rF1V plague vaccine with depot-formulated cytokines enhances immunogenicity and efficacy to elicit protective responses against aerosol challenge in mice.

Galloway, Darrell R / Li, Jiahui / Nguyen, Nguyen X / Falkenberg, Frank W / Henning, Lisa / Krile, Robert / Chou, Ying-Liang / Herron, James N / Hale, J Scott / Williamson, E Diane

Frontiers in immunology

2024 Volume 15, Page(s) 1277526

Abstract: This study evaluated a depot-formulated cytokine-based adjuvant to improve the efficacy of the recombinant F1V (rF1V) plague vaccine and examined the protective response following aerosol challenge in a murine model. The results of this study showed that ...

Abstract	This study evaluated a depot-formulated cytokine-based adjuvant to improve the efficacy of the recombinant F1V (rF1V) plague vaccine and examined the protective response following aerosol challenge in a murine model. The results of this study showed that co-formulation of the Alhydrogel-adsorbed rF1V plague fusion vaccine with the depot-formulated cytokines recombinant human interleukin 2 (rhuIL-2) and/or recombinant murine granulocyte macrophage colony-stimulating factor (rmGM-CSF) significantly enhances immunogenicity and significant protection at lower antigen doses against a lethal aerosol challenge. These results provide additional support for the co-application of the depot-formulated IL-2 and/or GM-CSF cytokines to enhance vaccine efficacy.
MeSH term(s)	Humans ; Animals ; Mice ; Plague Vaccine ; Cytokines ; Yersinia pestis ; Antigens, Bacterial ; Vaccines, Synthetic ; Aerosols
Chemical Substances	Plague Vaccine ; Cytokines ; Antigens, Bacterial ; Vaccines, Synthetic ; Aerosols
Language	English
Publishing date	2024-03-28
Publishing country	Switzerland
Document type	Journal Article ; Research Support, U.S. Gov't, P.H.S.
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2024.1277526
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Anthrax prophylaxis: recent advances and future directions.

Williamson, E Diane / Dyson, Edward Hugh

Frontiers in microbiology

2015 Volume 6, Page(s) 1009

Abstract: Anthrax is a serious, potentially fatal disease that can present in four distinct clinical patterns depending on the route of infection (cutaneous, gastrointestinal, pneumonic, or injectional); effective strategies for prophylaxis and therapy are ... ...

Abstract	Anthrax is a serious, potentially fatal disease that can present in four distinct clinical patterns depending on the route of infection (cutaneous, gastrointestinal, pneumonic, or injectional); effective strategies for prophylaxis and therapy are therefore required. This review addresses the complex mechanisms of pathogenesis employed by the bacterium and describes how, as understanding of these has developed over many years, so too have current strategies for vaccination and therapy. It covers the clinical and veterinary use of live attenuated strains of anthrax and the subsequent identification of protein sub-units for incorporation into vaccines, as well as combinations of protein sub-units with spore or other components. It also addresses the application of these vaccines for conventional prophylactic use, as well as post-exposure use in conjunction with antibiotics. It describes the licensed acellular vaccines AVA and AVP and discusses the prospects for a next generation of recombinant sub-unit vaccines for anthrax, balancing the regulatory requirement and current drive for highly defined vaccines, against the risk of losing the "danger" signals required to induce protective immunity in the vaccinee. It considers novel approaches to reduce time to immunity by means of combining, for example, dendritic cell vaccination with conventional approaches and considers current opportunities for the immunotherapy of anthrax.
Language	English
Publishing date	2015-09-24
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2015.01009
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Article: Early Isolates of SARS-CoV-2 Result in Different Pathogenesis in the Transduced Mouse Model of COVID-19

Smither, Sophie J. / Kempster, Sarah / Priestnall, Simon L. / Suárez-Bonnet, Alejandro / Stapleton, Helen / Laws, Thomas R. / Ferguson, Deborah / Almond, Neil / Lever, Mark S. / Williamson, E. Diane

Viruses. 2022 Aug. 13, v. 14, no. 8

2022

Abstract: A transduced mouse model of SARS-CoV-2 infection was established using Balb/c mice. This was achieved through the adenovirus-vectored delivery of the hACE2 gene, to render the mice transiently susceptible to the virus. The model was characterised in ... ...

Abstract	A transduced mouse model of SARS-CoV-2 infection was established using Balb/c mice. This was achieved through the adenovirus-vectored delivery of the hACE2 gene, to render the mice transiently susceptible to the virus. The model was characterised in terms of the dissemination of hACE2 receptor expression, the dissemination of three SARS-CoV-2 virus variants in vivo up to 10 days following challenge, the resulting histopathology and the clinical signs induced in the mice. In transduced mice, the infection was short-term, with a rapid loss in body weight starting at day 2 with maximum weight loss at day 4, followed by subsequent recovery until day 10. The induced expression of the hACE2 receptor was evident in the lungs, but, upon challenge, the SARS-CoV-2 virus disseminated beyond the lungs to spleen, liver and kidney, peaking at day 2 post infection. However, by day 10 post infection, the virus was undetectable. The lung histopathology was characterised by bronchial and alveolar inflammation, which was still present at day 10 post infection. Transduced mice had differential responses to viral variants ranking CVR-Glasgow 1 > Victoria-1 > England-2 isolates in terms of body weight loss. The transduced mouse model provides a consistent and manipulatable model of SARS-CoV-2 infection to screen viral variants for their relative virulence and possible interventions.
Keywords	COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; body weight changes ; genes ; histopathology ; inflammation ; kidneys ; liver ; lungs ; mice ; models ; pathogenesis ; spleen ; virulence ; viruses ; weight loss
Language	English
Dates of publication	2022-0813
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2516098-9
ISSN	1999-4915
ISSN	1999-4915
DOI	10.3390/v14081769
Database	NAL-Catalogue (AGRICOLA)

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Article: A self-amplifying RNA vaccine provides protection in a murine model of bubonic plague.

Shattock, Robin John / Andrianaivoarimanana, Voahangy / McKay, Paul F / Randriantseheno, Lovasoa Nomena / Murugaiah, Valarmathy / Samnuan, K / Rogers, Paul / Tregoning, John S / Rajerison, Minoarisoa / Moore, Kristoffer M / Laws, Thomas Robert / Williamson, E Diane

Frontiers in microbiology

2023 Volume 14, Page(s) 1247041

Abstract: Mice were immunized with a combination of self-amplifying (sa) RNA constructs for the F1 and V antigens ... ...

Abstract	Mice were immunized with a combination of self-amplifying (sa) RNA constructs for the F1 and V antigens of
Language	English
Publishing date	2023-11-02
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2587354-4
ISSN	1664-302X
ISSN	1664-302X
DOI	10.3389/fmicb.2023.1247041
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Article ; Online: Tumour Necrosis Factor-α, Chemokines, and Leukocyte Infiltrate Are Biomarkers for Pathology in the Brains of Venezuelan Equine Encephalitis (VEEV)-Infected Mice.

Phelps, Amanda L / Salguero, Francisco J / Hunter, Laura / Stoll, Alexander L / Jenner, Dominic C / O'Brien, Lyn M / Williamson, E Diane / Lever, M Stephen / Laws, Thomas R

Viruses

2023 Volume 15, Issue 6

Abstract: Venezuelan equine encephalitis virus (VEEV) is a disease typically confined to South and Central America, whereby human disease is characterised by a transient systemic infection and occasionally severe encephalitis, which is associated with lethality. ... ...

Abstract	Venezuelan equine encephalitis virus (VEEV) is a disease typically confined to South and Central America, whereby human disease is characterised by a transient systemic infection and occasionally severe encephalitis, which is associated with lethality. Using an established mouse model of VEEV infection, the encephalitic aspects of the disease were analysed to identify biomarkers associated with inflammation. Sequential sampling of lethally challenged mice (infected subcutaneously) confirmed a rapid onset systemic infection with subsequent spread to the brain within 24 h of the challenge. Changes in inflammatory biomarkers (TNF-α, CCL-2, and CCL-5) and CD45
MeSH term(s)	Humans ; Horses ; Mice ; Animals ; Encephalomyelitis, Venezuelan Equine ; Tumor Necrosis Factor-alpha ; Encephalitis Virus, Venezuelan Equine/physiology ; Brain ; Inflammation/pathology ; Chemokines ; Leukocytes
Chemical Substances	Tumor Necrosis Factor-alpha ; Chemokines
Language	English
Publishing date	2023-05-31
Publishing country	Switzerland
Document type	Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v15061307
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: The magnitude of the germinal center B cell and T follicular helper cell response predicts long-lasting antibody titers to plague vaccination.

Galloway, Darrell R / Nguyen, Nguyen X / Li, Jiahui / Houston, Nicholas / Gregersen, Gage / Williamson, E Diane / Falkenberg, Frank W / Herron, James N / Hale, J Scott

Frontiers in immunology

2022 Volume 13, Page(s) 1017385

Abstract: The development of a safe and effective vaccine ... ...

Abstract	The development of a safe and effective vaccine against
MeSH term(s)	Mice ; Animals ; Aluminum Hydroxide ; Germinal Center ; T Follicular Helper Cells ; Vaccination ; Adjuvants, Immunologic/pharmacology ; Vaccines, Subunit ; Immunization, Secondary ; Vaccines, Synthetic
Chemical Substances	Aluminum Hydroxide (5QB0T2IUN0) ; Adjuvants, Immunologic ; Vaccines, Subunit ; Vaccines, Synthetic
Language	English
Publishing date	2022-10-28
Publishing country	Switzerland
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2022.1017385
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Early Isolates of SARS-CoV-2 Result in Different Pathogenesis in the Transduced Mouse Model of COVID-19.

Smither, Sophie J / Kempster, Sarah / Priestnall, Simon L / Suárez-Bonnet, Alejandro / Stapleton, Helen / Laws, Thomas R / Ferguson, Deborah / Almond, Neil / Lever, Mark S / Williamson, E Diane

Viruses

2022 Volume 14, Issue 8

Abstract	A transduced mouse model of SARS-CoV-2 infection was established using Balb/c mice. This was achieved through the adenovirus-vectored delivery of the hACE2 gene, to render the mice transiently susceptible to the virus. The model was characterised in terms of the dissemination of hACE2 receptor expression, the dissemination of three SARS-CoV-2 virus variants in vivo up to 10 days following challenge, the resulting histopathology and the clinical signs induced in the mice. In transduced mice, the infection was short-term, with a rapid loss in body weight starting at day 2 with maximum weight loss at day 4, followed by subsequent recovery until day 10. The induced expression of the hACE2 receptor was evident in the lungs, but, upon challenge, the SARS-CoV-2 virus disseminated beyond the lungs to spleen, liver and kidney, peaking at day 2 post infection. However, by day 10 post infection, the virus was undetectable. The lung histopathology was characterised by bronchial and alveolar inflammation, which was still present at day 10 post infection. Transduced mice had differential responses to viral variants ranking CVR-Glasgow 1 > Victoria-1 > England-2 isolates in terms of body weight loss. The transduced mouse model provides a consistent and manipulatable model of SARS-CoV-2 infection to screen viral variants for their relative virulence and possible interventions.
MeSH term(s)	Angiotensin-Converting Enzyme 2/genetics ; Animals ; COVID-19 ; Disease Models, Animal ; Lung ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Peptidyl-Dipeptidase A/metabolism ; SARS-CoV-2/genetics
Chemical Substances	Peptidyl-Dipeptidase A (EC 3.4.15.1) ; Angiotensin-Converting Enzyme 2 (EC 3.4.17.23)
Language	English
Publishing date	2022-08-13
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v14081769
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Serological responses to Anthrax Vaccine Precipitated (AVP) increase with time interval between booster doses.

Dyson, E Hugh / Simpson, Andrew J H / Gwyther, Robert J / Cuthbertson, Hannah / Patient, Dawn H / Matheson, Mary / Gregg, Anya / Hepburn, Matthew J / Hallis, Bassam / Williamson, E Diane

Vaccine

2022 Volume 40, Issue 42, Page(s) 6163–6178

Abstract: We undertook a Phase 4 clinical trial to assess the effect of time interval between booster doses on serological responses to AVP. The primary objective was to evaluate responses to a single booster dose in two groups of healthy adults who had previously ...

Abstract	We undertook a Phase 4 clinical trial to assess the effect of time interval between booster doses on serological responses to AVP. The primary objective was to evaluate responses to a single booster dose in two groups of healthy adults who had previously received a complete 4-dose primary course. Group A had received doses on schedule while Group B had not had one for ≥2 years. Secondary objectives were to evaluate the safety and tolerability of AVP booster doses, and to gain information on correlates of protection to aid future anthrax vaccine development. Blood samples were taken on Day 1 before dosing, and on Days 8, 15, 29 and 120, to measure Toxin Neutralisation Assay (TNA) NF
MeSH term(s)	Adult ; Anthrax/prevention & control ; Anthrax Vaccines ; Antibodies, Bacterial ; Antigens, Bacterial ; Bacillus anthracis ; Humans ; Immunoglobulin G ; Vaccination/methods
Chemical Substances	Anthrax Vaccines ; Antibodies, Bacterial ; Antigens, Bacterial ; Immunoglobulin G
Language	English
Publishing date	2022-09-22
Publishing country	Netherlands
Document type	Clinical Trial, Phase IV ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605674-x
ISSN	1873-2518 ; 0264-410X
ISSN (online)	1873-2518
ISSN	0264-410X
DOI	10.1016/j.vaccine.2022.08.052
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Zs.A 1930: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 458: Show issues

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Article: Impact of HLA Polymorphism on the Immune Response to Bacillus Anthracis Protective Antigen in Vaccination versus Natural Infection.

Ascough, Stephanie / Ingram, Rebecca J / Chu, Karen K Y / Moore, Stephen J / Gallagher, Theresa / Dyson, Hugh / Doganay, Mehmet / Metan, Gökhan / Ozkul, Yusuf / Baillie, Les / Williamson, E Diane / Robinson, John H / Maillere, Bernard / Boyton, Rosemary J / Altmann, Daniel M

Vaccines

2022 Volume 10, Issue 10

Abstract: The causative agent of anthrax, Bacillus anthracis, evades the host immune response and establishes infection through the production of binary exotoxins composed of Protective Antigen (PA) and one of two subunits, lethal factor (LF) or edema factor (EF). ...

Abstract	The causative agent of anthrax, Bacillus anthracis, evades the host immune response and establishes infection through the production of binary exotoxins composed of Protective Antigen (PA) and one of two subunits, lethal factor (LF) or edema factor (EF). The majority of vaccination strategies have focused upon the antibody response to the PA subunit. We have used a panel of humanised HLA class II transgenic mouse strains to define HLA-DR-restricted and HLA-DQ-restricted CD4+ T cell responses to the immunodominant epitopes of PA. This was correlated with the binding affinities of epitopes to HLA class II molecules, as well as the responses of two human cohorts: individuals vaccinated with the Anthrax Vaccine Precipitated (AVP) vaccine (which contains PA and trace amounts of LF), and patients recovering from cutaneous anthrax infections. The infected and vaccinated cohorts expressing different HLA types were found to make CD4+ T cell responses to multiple and diverse epitopes of PA. The effects of HLA polymorphism were explored using transgenic mouse lines, which demonstrated differential susceptibility, indicating that HLA-DR1 and HLA-DQ8 alleles conferred protective immunity relative to HLA-DR15, HLA-DR4 and HLA-DQ6. The HLA transgenics enabled a reductionist approach, allowing us to better define CD4+ T cell epitopes. Appreciating the effects of HLA polymorphism on the variability of responses to natural infection and vaccination is vital in planning protective strategies against anthrax.
Language	English
Publishing date	2022-09-20
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2703319-3
ISSN	2076-393X
ISSN	2076-393X
DOI	10.3390/vaccines10101571
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