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  1. Article ; Online: Reviews 2020: The state of the art in separations applied to the life sciences.

    Wilson, Ian D

    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    2021  Volume 1173, Page(s) 122679

    Language English
    Publishing date 2021-03-29
    Publishing country Netherlands
    Document type Editorial
    ZDB-ID 1180823-8
    ISSN 1873-376X ; 0378-4347 ; 1570-0232 ; 1387-2273
    ISSN (online) 1873-376X
    ISSN 0378-4347 ; 1570-0232 ; 1387-2273
    DOI 10.1016/j.jchromb.2021.122679
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  2. Article ; Online: The state of the art in planar chromatography.

    Poole, Colin / Wilson, Ian D

    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    2022  Volume 1203, Page(s) 123309

    MeSH term(s) Chromatography, Thin Layer/methods
    Language English
    Publishing date 2022-05-21
    Publishing country Netherlands
    Document type Editorial
    ZDB-ID 1180823-8
    ISSN 1873-376X ; 0378-4347 ; 1570-0232 ; 1387-2273
    ISSN (online) 1873-376X
    ISSN 0378-4347 ; 1570-0232 ; 1387-2273
    DOI 10.1016/j.jchromb.2022.123309
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  3. Article ; Online: Planar chromatography - Current practice and future prospects.

    Wilson, Ian D / Poole, Colin F

    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    2022  Volume 1214, Page(s) 123553

    Abstract: Planar chromatography, in the form of thin-layer or high-performance thin-layer chromatography (TLC, HPTLC), continues to provide a robust and widely used separation technique. It is unrivaled as a simple and rapid qualitative method for mixture analysis, ...

    Abstract Planar chromatography, in the form of thin-layer or high-performance thin-layer chromatography (TLC, HPTLC), continues to provide a robust and widely used separation technique. It is unrivaled as a simple and rapid qualitative method for mixture analysis, or for finding bioactive components in mixtures. The format of TLC/HPTLC also provides a unique method for preserving the separation, enabling further investigation of components of interest (including quantification/structure determination) separated in both time and space from the original analysis. The current practice of planar chromatography and areas of development of the technology are reviewed and promising future directions in the use of TLC/HPTLC are outlined.
    Language English
    Publishing date 2022-11-28
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 1180823-8
    ISSN 1873-376X ; 0378-4347 ; 1570-0232 ; 1387-2273
    ISSN (online) 1873-376X
    ISSN 0378-4347 ; 1570-0232 ; 1387-2273
    DOI 10.1016/j.jchromb.2022.123553
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  4. Article ; Online: Application of hybrid surface technology for improving sensitivity and peak shape of phosphorylated lipids such as phosphatidic acid and phosphatidylserine.

    Isaac, Giorgis / Wilson, Ian D / Plumb, Robert S

    Journal of chromatography. A

    2022  Volume 1669, Page(s) 462921

    Abstract: The use of hybrid surface technology (HST), applied to the metal surfaces of an ACQUITY™ UPLC™ system and column, designed to mitigate the chelation, poor peak shape and analyte loss seen with acidic phospholipids was investigated. Compared to a ... ...

    Abstract The use of hybrid surface technology (HST), applied to the metal surfaces of an ACQUITY™ UPLC™ system and column, designed to mitigate the chelation, poor peak shape and analyte loss seen with acidic phospholipids was investigated. Compared to a conventional system significant improvements in both sensitivity, recovery and peak shape were obtained following UPLC on a CSH C18 column when the HST was used for the analysis of lysophosphatidic acid (LPA), phosphatidic acid (PA), lysophosphatidylserine (LPS), phosphatidylserine (PS), phosphatidylinositol-monophosphates (PIP), ceramide phosphate (CerP) and sphingoid base phosphate (SPBP). The benefits in chromatographic performance provided by the HST were seen particularly at low concentrations of these analytes. The HST system and column reduced peak tailing by 65-80% and peak width by 70-86% for LPA and PA. Moreover, increased signal intensities of up to 12.7 times were observed for LPA with the HST approach compared to the equivalent untreated LC system and column. The application of this methodology to the analysis of chicken egg PA and brain porcine PS extracts were accompanied by similar improvements in data quality.
    MeSH term(s) Animals ; Metals/chemistry ; Phosphatidic Acids ; Phosphatidylinositols ; Phosphatidylserines/analysis ; Swine ; Technology
    Chemical Substances Metals ; Phosphatidic Acids ; Phosphatidylinositols ; Phosphatidylserines
    Language English
    Publishing date 2022-02-25
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1171488-8
    ISSN 1873-3778 ; 0021-9673
    ISSN (online) 1873-3778
    ISSN 0021-9673
    DOI 10.1016/j.chroma.2022.462921
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  5. Article: Application of hybrid surface technology for improving sensitivity and peak shape of phosphorylated lipids such as phosphatidic acid and phosphatidylserine

    Isaac, Giorgis / Wilson, Ian D / Plumb, Robert S.

    Journal of chromatography. 2022 Apr. 26, v. 1669

    2022  

    Abstract: The use of hybrid surface technology (HST), applied to the metal surfaces of an ACQUITY™ UPLC™ system and column, designed to mitigate the chelation, poor peak shape and analyte loss seen with acidic phospholipids was investigated. Compared to a ... ...

    Abstract The use of hybrid surface technology (HST), applied to the metal surfaces of an ACQUITY™ UPLC™ system and column, designed to mitigate the chelation, poor peak shape and analyte loss seen with acidic phospholipids was investigated. Compared to a conventional system significant improvements in both sensitivity, recovery and peak shape were obtained following UPLC on a CSH C18 column when the HST was used for the analysis of lysophosphatidic acid (LPA), phosphatidic acid (PA), lysophosphatidylserine (LPS), phosphatidylserine (PS), phosphatidylinositol-monophosphates (PIP), ceramide phosphate (CerP) and sphingoid base phosphate (SPBP). The benefits in chromatographic performance provided by the HST were seen particularly at low concentrations of these analytes. The HST system and column reduced peak tailing by 65–80% and peak width by 70–86% for LPA and PA. Moreover, increased signal intensities of up to 12.7 times were observed for LPA with the HST approach compared to the equivalent untreated LC system and column. The application of this methodology to the analysis of chicken egg PA and brain porcine PS extracts were accompanied by similar improvements in data quality.
    Keywords ceramides ; chelation ; chemical species ; chickens ; chromatography ; data quality ; eggs ; lysophospholipids ; phosphates ; phosphatidylserines ; swine
    Language English
    Dates of publication 2022-0426
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 218139-3
    ISSN 0021-9673 ; 0378-4355 ; 0376-737X
    ISSN 0021-9673 ; 0378-4355 ; 0376-737X
    DOI 10.1016/j.chroma.2022.462921
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  6. Article ; Online: Ensuring Fact-Based Metabolite Identification in Liquid Chromatography-Mass Spectrometry-Based Metabolomics

    Theodoridis, Georgios / Gika, Helen / Raftery, Daniel / Goodacre, Royston / Plumb, Robert S. / Wilson, Ian D.

    Analytical Chemistry. 2023 Feb. 15, v. 95, no. 8 p.3909-3916

    2023  

    Abstract: Metabolite identification represents a major bottleneck in contemporary metabolomics research and a step where critical errors may occur and pass unnoticed. This is especially the case for studies employing liquid chromatography-mass spectrometry ... ...

    Abstract Metabolite identification represents a major bottleneck in contemporary metabolomics research and a step where critical errors may occur and pass unnoticed. This is especially the case for studies employing liquid chromatography-mass spectrometry technology, where there is increased concern on the validity of the proposed identities. In the present perspective article, we describe the issue and categorize the errors into two types: identities that show poor biological plausibility and identities that do not comply with chromatographic data and thus to physicochemical properties (usually hydrophobicity/hydrophilicity) of the proposed molecule. We discuss the problem, present characteristic examples, and propose measures to improve the situation.
    Keywords analytical chemistry ; hydrophilicity ; hydrophobicity ; liquid chromatography ; mass spectrometry ; metabolites ; metabolomics
    Language English
    Dates of publication 2023-0215
    Size p. 3909-3916.
    Publishing place American Chemical Society
    Document type Article ; Online
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.2c05192
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  7. Article ; Online: Access to the Phospho-proteome via the Mitigation of Peptide-Metal Interactions.

    Hughes, Christopher J / Gethings, Lee A / Wilson, Ian D / Plumb, Robert S

    Journal of chromatography. A

    2022  Volume 1673, Page(s) 463024

    Abstract: Liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS) has become the default platform for proteomics due to its specificity, sensitivity and sample compatibility. However, interactions between transition metals in LC systems and ... ...

    Abstract Liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS) has become the default platform for proteomics due to its specificity, sensitivity and sample compatibility. However, interactions between transition metals in LC systems and analytes containing phosphate groups result in poor chromatographic performance or even analyte loss. The use of systems where the metal surfaces had been treated with a hybrid inorganic/organic surface material to form an effective surface barrier mitigated these undesired interactions. When employed for the analysis of tryptic digests of Alpha and Beta Casein, along with synthetic "PhosphoMix" standards, the use of such a system showed significant improvements in chromatographic peak shape and analyte response together with superior spectral quality and sequence coverage. These improvements resulted in the detection of phosphorylated peptides ADEPSSESDLEIDK and ELSNSPLRENSFGSPLEFR from the PhosphoMix which went undetected with a standard, untreated LC system. The hybrid surface system thus offered significant advantages for the analysis of phosphopeptides compared to conventional LC/MS.
    MeSH term(s) Chromatography, Liquid/methods ; Mass Spectrometry/methods ; Metals ; Phosphopeptides/chemistry ; Proteome ; Proteomics/methods
    Chemical Substances Metals ; Phosphopeptides ; Proteome
    Language English
    Publishing date 2022-04-04
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1171488-8
    ISSN 1873-3778 ; 0021-9673
    ISSN (online) 1873-3778
    ISSN 0021-9673
    DOI 10.1016/j.chroma.2022.463024
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  8. Article ; Online: The 44th annual open meeting of the Drug Metabolism Discussion Group (DMDG) at Robinson College, Cambridge, UK, September 16th-18th, 2015.

    Wilson, Ian D

    Xenobiotica; the fate of foreign compounds in biological systems

    2015  Volume 46, Issue 7, Page(s) 664–666

    Language English
    Publishing date 2015-11-02
    Publishing country England
    Document type Journal Article
    ZDB-ID 120287-x
    ISSN 1366-5928 ; 0049-8254
    ISSN (online) 1366-5928
    ISSN 0049-8254
    DOI 10.3109/00498254.2016.1164798
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  9. Article ; Online: High performance Reversed-Phase Thin-Layer Chromatography-Desorption electrospray ionisation - time of flight high resolution mass spectrometric detection and imaging (HPTLC/DESI/ToFMS) of phytoecdysteroids.

    Claude, Emmanuelle / Lafont, Rene / Plumb, Robert S / Wilson, Ian D

    Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

    2022  Volume 1200, Page(s) 123265

    Abstract: Reversed-phase high performance thin-layer chromatography (RP-HPTLC) on C18 bonded silica gel was combined with desorption electrospray ionization (DESI) and high resolution time of flight mass spectrometry (HRToFMS) to detect, characterize and image ( ... ...

    Abstract Reversed-phase high performance thin-layer chromatography (RP-HPTLC) on C18 bonded silica gel was combined with desorption electrospray ionization (DESI) and high resolution time of flight mass spectrometry (HRToFMS) to detect, characterize and image (MSI) phytoecdysteroids (plant-derived insect moulting hormones) in ethanolic extracts of members of the Silene plant family. As seen previously for silica gel, DESI provided a simple and convenient method for recovering polar polyhydoxysteroids from RP-HPTLC plates for the purposes of both the MS and MSI of extracts obtained from three species of the Silene family (Silene otites, S. nutans and S. viridiflora). Using RP-HPTLC/DESI/MSI/HRToFMS a number of ecdysteroids, including 20-hydroxyecdysone, polypodine-B, 2-deoxy-20-hydroxyecdysone and 2-deoxyecdysone were identified in these extracts. Differences were noted in the mass spectra obtained depending upon both the stationary phase on which they were separated, and the temperatures used in the heated transfer line used for introduction into the ion source. Ecdysteroids detected after chromatography on C18 bonded silica showed increased fragmentation due to water loss compared to those imaged from silica. In addition, the benefits of the additional resolution provided by 2-dimensional TLC for increasing spectral quality compared to a 1-dimensional separation are demonstrated.
    MeSH term(s) Chromatography, Thin Layer/methods ; Ecdysteroids ; Ecdysterone ; Plant Extracts/chemistry ; Silica Gel ; Spectrometry, Mass, Electrospray Ionization/methods
    Chemical Substances Ecdysteroids ; Plant Extracts ; Ecdysterone (5289-74-7) ; Silica Gel (60650-90-0)
    Language English
    Publishing date 2022-04-27
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1180823-8
    ISSN 1873-376X ; 0378-4347 ; 1570-0232 ; 1387-2273
    ISSN (online) 1873-376X
    ISSN 0378-4347 ; 1570-0232 ; 1387-2273
    DOI 10.1016/j.jchromb.2022.123265
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  10. Article ; Online: Metabolic phenotyping by liquid chromatography-mass spectrometry to study human health and disease.

    Wilson, Ian D

    Analytical chemistry

    2015  Volume 87, Issue 5, Page(s) 2519

    MeSH term(s) Chromatography, Liquid/methods ; Disease ; Humans ; Mass Spectrometry/methods ; Metabolome/physiology ; Metabolomics/methods ; Models, Biological
    Language English
    Publishing date 2015-03-03
    Publishing country United States
    Document type Editorial ; Introductory Journal Article
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.5b00409
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