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  1. Article ; Online: The Marine Natural Product Furospinulosin 1 Induces Apoptosis in MDA-MB-231 Triple Negative Breast Cancer Cell Spheroids, But Not in Cells Grown Traditionally with Longer Treatment.

    Guzmán, Esther A / Pitts, Tara P / Winder, Priscilla L / Wright, Amy E

    Marine drugs

    2021  Volume 19, Issue 5

    Abstract: Cancer cells grown in spheroid conditions interact with each other and the extracellular matrix, providing a better representation of the in vivo environment than two-dimensional cultures and are a more clinically relevant model. A discrete screening of ... ...

    Abstract Cancer cells grown in spheroid conditions interact with each other and the extracellular matrix, providing a better representation of the in vivo environment than two-dimensional cultures and are a more clinically relevant model. A discrete screening of genetically diverse marine samples in the spheroid assay led to the identification of a novel activity for the known compound furospinulosin 1. This compound shows activity against MDA-MB-231 triple negative breast cancer cells grown as spheroids and treated for 24 or 48 h. No cytotoxicity was seen in traditional two-dimensional adherent cultures treated for a longer time (72 h). A reverse phase protein array (RPPA) confirmed the limited activity of the compound in cells grown traditionally and revealed changes in protein expression when cells are grown as spheroids that are associated with better clinical prognosis. Analysis of the RPPA data through the Broad institute's connectivity map suggested the hypothesis that furospinulosin 1 functions as an MEK inhibitor. Analysis of the RPPA data through STRING supports the apoptosis observed. The selectivity exhibited by furospinulosin 1 for triple negative breast cancer cells only when grown as spheroids makes it an interesting compound with strong therapeutic potential that merits further study.
    MeSH term(s) Antineoplastic Agents/pharmacology ; Apoptosis/drug effects ; Cell Culture Techniques ; Cell Line, Tumor ; Computational Biology ; Female ; Humans ; Protein Interaction Maps ; Proteome ; Proteomics ; Sesterterpenes/pharmacology ; Signal Transduction ; Spheroids, Cellular ; Time Factors ; Triple Negative Breast Neoplasms/drug therapy ; Triple Negative Breast Neoplasms/metabolism ; Triple Negative Breast Neoplasms/pathology
    Chemical Substances Antineoplastic Agents ; Proteome ; Sesterterpenes ; furospinosulin-1
    Language English
    Publishing date 2021-04-28
    Publishing country Switzerland
    Document type Comparative Study ; Journal Article
    ZDB-ID 2175190-0
    ISSN 1660-3397 ; 1660-3397
    ISSN (online) 1660-3397
    ISSN 1660-3397
    DOI 10.3390/md19050249
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: An Assessment of Potential Threats to Human Health from Algae Blooms in the Indian River Lagoon (USA) 2018-2021: Unique Patterns of Cytotoxicity Associated with Toxins.

    Guzmán, Esther A / Peterson, Tara A / Winder, Priscilla L / Francis, Kirstie T / McFarland, Malcolm / Roberts, Jill C / Sandle, Jennifer / Wright, Amy E

    Toxins

    2023  Volume 15, Issue 11

    Abstract: The Indian River Lagoon (IRL), a 156-mile-long estuary located on the eastern coast of Florida, experiences phytoplankton bloom events due to increased seasonal temperatures coupled with anthropogenic impacts. This study aimed to gather data on the ... ...

    Abstract The Indian River Lagoon (IRL), a 156-mile-long estuary located on the eastern coast of Florida, experiences phytoplankton bloom events due to increased seasonal temperatures coupled with anthropogenic impacts. This study aimed to gather data on the toxicity to human cells and to identify secondary metabolites found in water samples collected in the IRL. Water samples from 20 sites of the IRL were collected during the wet and dry seasons over a three-year period. A panel of cell lines was used to test cytotoxicity. Hemagglutination, hemolysis, and inhibition of protein phosphatase 2A (PP2A) were also measured. Cytotoxic blooms were seen both in the south (
    MeSH term(s) Humans ; Rivers ; Dinoflagellida/physiology ; Eutrophication ; Saxitoxin ; Toxins, Biological ; Water ; Harmful Algal Bloom
    Chemical Substances Saxitoxin (35523-89-8) ; Toxins, Biological ; Water (059QF0KO0R)
    Language English
    Publishing date 2023-11-17
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2518395-3
    ISSN 2072-6651 ; 2072-6651
    ISSN (online) 2072-6651
    ISSN 2072-6651
    DOI 10.3390/toxins15110664
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: The Marine Natural Product Furospinulosin 1 Induces Apoptosis in MDA-MB-231 Triple Negative Breast Cancer Cell Spheroids, But Not in Cells Grown Traditionally with Longer Treatment

    Guzmán, Esther A / Pitts, Tara P / Winder, Priscilla L / Wright, Amy E

    Marine drugs. 2021 Apr. 28, v. 19, no. 5

    2021  

    Abstract: Cancer cells grown in spheroid conditions interact with each other and the extracellular matrix, providing a better representation of the in vivo environment than two-dimensional cultures and are a more clinically relevant model. A discrete screening of ... ...

    Abstract Cancer cells grown in spheroid conditions interact with each other and the extracellular matrix, providing a better representation of the in vivo environment than two-dimensional cultures and are a more clinically relevant model. A discrete screening of genetically diverse marine samples in the spheroid assay led to the identification of a novel activity for the known compound furospinulosin 1. This compound shows activity against MDA-MB-231 triple negative breast cancer cells grown as spheroids and treated for 24 or 48 h. No cytotoxicity was seen in traditional two-dimensional adherent cultures treated for a longer time (72 h). A reverse phase protein array (RPPA) confirmed the limited activity of the compound in cells grown traditionally and revealed changes in protein expression when cells are grown as spheroids that are associated with better clinical prognosis. Analysis of the RPPA data through the Broad institute’s connectivity map suggested the hypothesis that furospinulosin 1 functions as an MEK inhibitor. Analysis of the RPPA data through STRING supports the apoptosis observed. The selectivity exhibited by furospinulosin 1 for triple negative breast cancer cells only when grown as spheroids makes it an interesting compound with strong therapeutic potential that merits further study.
    Keywords apoptosis ; breast neoplasms ; cytotoxicity ; extracellular matrix ; models ; neoplasm cells ; prognosis ; protein synthesis ; therapeutics
    Language English
    Dates of publication 2021-0428
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 2175190-0
    ISSN 1660-3397
    ISSN 1660-3397
    DOI 10.3390/md19050249
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Discovery of Survivin Inhibitors Part 1: Screening the Harbor Branch Pure Compound Library.

    Guzmán, Esther A / Pitts, Tara P / Tandberg, Kirstie R / Winder, Priscilla L / Wright, Amy E

    Marine drugs

    2021  Volume 19, Issue 2

    Abstract: Survivin is a 16.5 KDa protein whose functions include promoting cellular mitosis, angiogenesis, and senescence as well as inhibiting apoptosis. Higher survivin expression is found in cancer tissues than normal tissues, and this expression correlates ... ...

    Abstract Survivin is a 16.5 KDa protein whose functions include promoting cellular mitosis, angiogenesis, and senescence as well as inhibiting apoptosis. Higher survivin expression is found in cancer tissues than normal tissues, and this expression correlates with disease progression and aggressiveness. Survivin has been validated as a clinical target for cancer. Small molecules are important antagonists of survivin levels in cancer cells. A structurally diverse library of genetically encoded small molecules (natural products) derived from marine plants, invertebrates, and microbes was screened for their ability to reduce expression levels of survivin in the DLD-1 colon adenocarcinoma and the A549 nonsmall cell lung carcinoma cell lines. This led to the identification of this novel activity for the known compounds eryloside E, ilicicolin H, tanzawaic acid A, and
    MeSH term(s) A549 Cells ; Antineoplastic Agents/pharmacology ; Apoptosis/drug effects ; Drug Evaluation, Preclinical/methods ; Fatty Acids, Unsaturated/pharmacology ; Humans ; Marine Biology ; Naphthalenes/pharmacology ; Survivin/antagonists & inhibitors
    Chemical Substances Antineoplastic Agents ; Fatty Acids, Unsaturated ; Naphthalenes ; Survivin ; tanzawaic acid A
    Language English
    Publishing date 2021-01-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2175190-0
    ISSN 1660-3397 ; 1660-3397
    ISSN (online) 1660-3397
    ISSN 1660-3397
    DOI 10.3390/md19020073
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Discovery of Survivin Inhibitors Part 1: Screening the Harbor Branch Pure Compound Library

    Guzmán, Esther A. / Pitts, Tara P. / Tandberg, Kirstie R. / Winder, Priscilla L. / Wright, Amy E.

    Marine drugs. 2021 Jan. 30, v. 19, no. 2

    2021  

    Abstract: Survivin is a 16.5 KDa protein whose functions include promoting cellular mitosis, angiogenesis, and senescence as well as inhibiting apoptosis. Higher survivin expression is found in cancer tissues than normal tissues, and this expression correlates ... ...

    Abstract Survivin is a 16.5 KDa protein whose functions include promoting cellular mitosis, angiogenesis, and senescence as well as inhibiting apoptosis. Higher survivin expression is found in cancer tissues than normal tissues, and this expression correlates with disease progression and aggressiveness. Survivin has been validated as a clinical target for cancer. Small molecules are important antagonists of survivin levels in cancer cells. A structurally diverse library of genetically encoded small molecules (natural products) derived from marine plants, invertebrates, and microbes was screened for their ability to reduce expression levels of survivin in the DLD-1 colon adenocarcinoma and the A549 nonsmall cell lung carcinoma cell lines. This led to the identification of this novel activity for the known compounds eryloside E, ilicicolin H, tanzawaic acid A, and p-hydroxyphenopyrrozin. Both eryloside E and ilicicolin H showed the ability to reduce survivin expression in the low micromolar range against both cell lines.
    Keywords adenocarcinoma ; angiogenesis ; apoptosis ; colon ; disease progression ; lung neoplasms ; mitosis ; neoplasm cells ; pro-apoptotic proteins
    Language English
    Dates of publication 2021-0130
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2175190-0
    ISSN 1660-3397
    ISSN 1660-3397
    DOI 10.3390/md19020073
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: Antiplasmodial Compounds from Deep-Water Marine Invertebrates

    Wright, Amy E / Collins, Jennifer E / Roberts, Bracken / Roberts, Jill C / Winder, Priscilla L / Reed, John K / Diaz, Maria Cristina / Pomponi, Shirley A / Chakrabarti, Debopam

    Marine drugs. 2021 Mar. 25, v. 19, no. 4

    2021  

    Abstract: Novel drug leads for malaria therapy are urgently needed because of the widespread emergence of resistance to all available drugs. Screening of the Harbor Branch enriched fraction library against the Plasmodium falciparum chloroquine-resistant strain ( ... ...

    Abstract Novel drug leads for malaria therapy are urgently needed because of the widespread emergence of resistance to all available drugs. Screening of the Harbor Branch enriched fraction library against the Plasmodium falciparum chloroquine-resistant strain (Dd2) followed by bioassay-guided fractionation led to the identification of two potent antiplasmodials; a novel diterpene designated as bebrycin A (1) and the known C21 degraded terpene nitenin (2). A SYBR Green I assay was used to establish a Dd2 EC₅₀ of 1.08 ± 0.21 and 0.29 ± 0.02 µM for bebrycin A and nitenin, respectively. Further analysis was then performed to assess the stage specificity of the inhibitors antiplasmodial effects on the Dd2 intraerythrocytic life cycle. Exposure to bebrycin A was found to block parasite maturation at the schizont stage if added any time prior to late schizogony at 42 hours post invasion, (HPI). In contrast, early life cycle exposure to nitenin (prior to 18 HPI) was identified as crucial to parasite inhibition, suggesting nitenin may target the maturation of the parasite during the transition from ring to early trophozoite (6–18 HPI), a novel property among known antimalarials.
    Keywords Plasmodium falciparum ; antimalarials ; diterpenoids ; fractionation ; malaria ; parasites ; schizogony ; therapeutics
    Language English
    Dates of publication 2021-0325
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 2175190-0
    ISSN 1660-3397
    ISSN 1660-3397
    DOI 10.3390/md19040179
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: High-Throughput Screening of a Marine Compound Library Identifies Anti-

    Bone Relat, Rachel M / Winder, Priscilla L / Bowden, Gregory D / Guzmán, Esther A / Peterson, Tara A / Pomponi, Shirley A / Roberts, Jill C / Wright, Amy E / O'Connor, Roberta M

    Marine drugs

    2022  Volume 20, Issue 4

    Abstract: ... ...

    Abstract Cryptosporidium
    MeSH term(s) Animals ; Biological Products/pharmacology ; Cell Line ; Chlorocebus aethiops ; Cryptosporidiosis/parasitology ; Cryptosporidium ; Cryptosporidium parvum ; High-Throughput Screening Assays ; Humans
    Chemical Substances Biological Products
    Language English
    Publishing date 2022-03-30
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2175190-0
    ISSN 1660-3397 ; 1660-3397
    ISSN (online) 1660-3397
    ISSN 1660-3397
    DOI 10.3390/md20040240
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Plakinamine P, A Steroidal Alkaloid with Bactericidal Activity against

    Rodrigues Felix, Carolina / Roberts, Jill C / Winder, Priscilla L / Gupta, Rashmi / Diaz, M Cristina / Pomponi, Shirley A / Wright, Amy E / Rohde, Kyle H

    Marine drugs

    2019  Volume 17, Issue 12

    Abstract: Tuberculosis is the leading cause of death due to infectious disease worldwide. There is an urgent need for more effective compounds against this pathogen to control the disease. Investigation of the anti-mycobacterial activity of a deep-water sponge of ... ...

    Abstract Tuberculosis is the leading cause of death due to infectious disease worldwide. There is an urgent need for more effective compounds against this pathogen to control the disease. Investigation of the anti-mycobacterial activity of a deep-water sponge of the genus
    MeSH term(s) Alkaloids/chemistry ; Alkaloids/pharmacology ; Antitubercular Agents/chemistry ; Antitubercular Agents/pharmacology ; Molecular Structure ; Mycobacterium tuberculosis/drug effects ; Steroids/chemistry ; Steroids/pharmacology
    Chemical Substances Alkaloids ; Antitubercular Agents ; Steroids
    Language English
    Publishing date 2019-12-16
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2175190-0
    ISSN 1660-3397 ; 1660-3397
    ISSN (online) 1660-3397
    ISSN 1660-3397
    DOI 10.3390/md17120707
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Antiplasmodial Compounds from Deep-Water Marine Invertebrates.

    Wright, Amy E / Collins, Jennifer E / Roberts, Bracken / Roberts, Jill C / Winder, Priscilla L / Reed, John K / Diaz, Maria Cristina / Pomponi, Shirley A / Chakrabarti, Debopam

    Marine drugs

    2021  Volume 19, Issue 4

    Abstract: Novel drug leads for malaria therapy are urgently needed because of the widespread emergence of resistance to all available drugs. Screening of the Harbor Branch enriched fraction library against ... ...

    Abstract Novel drug leads for malaria therapy are urgently needed because of the widespread emergence of resistance to all available drugs. Screening of the Harbor Branch enriched fraction library against the
    MeSH term(s) Animals ; Anthozoa/metabolism ; Antimalarials/isolation & purification ; Antimalarials/pharmacology ; Diterpenes/isolation & purification ; Diterpenes/pharmacology ; Hep G2 Cells ; Humans ; Life Cycle Stages ; Malaria, Falciparum/drug therapy ; Malaria, Falciparum/parasitology ; Molecular Structure ; Plasmodium falciparum/drug effects ; Plasmodium falciparum/growth & development ; Porifera/metabolism ; Structure-Activity Relationship ; Time Factors
    Chemical Substances Antimalarials ; Diterpenes
    Language English
    Publishing date 2021-03-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2175190-0
    ISSN 1660-3397 ; 1660-3397
    ISSN (online) 1660-3397
    ISSN 1660-3397
    DOI 10.3390/md19040179
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Natural products from the Lithistida: a review of the literature since 2000.

    Winder, Priscilla L / Pomponi, Shirley A / Wright, Amy E

    Marine drugs

    2011  Volume 9, Issue 12, Page(s) 2643–2682

    Abstract: Lithistid sponges are known to produce a diverse array of compounds ranging from polyketides, cyclic and linear peptides, alkaloids, pigments, lipids, and sterols. A majority of these structurally complex compounds have very potent and interesting ... ...

    Abstract Lithistid sponges are known to produce a diverse array of compounds ranging from polyketides, cyclic and linear peptides, alkaloids, pigments, lipids, and sterols. A majority of these structurally complex compounds have very potent and interesting biological activities. It has been a decade since a thorough review has been published that summarizes the literature on the natural products reported from this amazing sponge order. This review provides an update on the current taxonomic classification of the Lithistida, describes structures and biological activities of 131 new natural products, and discusses highlights from the total syntheses of 16 compounds from marine sponges of the Order Lithistida providing a compilation of the literature since the last review published in 2002.
    MeSH term(s) Animals ; Biological Products/chemical synthesis ; Biological Products/chemistry ; Biological Products/isolation & purification ; Biological Products/pharmacology ; HCT116 Cells ; Humans ; Porifera/chemistry ; Porifera/classification ; Time Factors
    Chemical Substances Biological Products
    Language English
    Publishing date 2011-12-15
    Publishing country Switzerland
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2175190-0
    ISSN 1660-3397 ; 1660-3397
    ISSN (online) 1660-3397
    ISSN 1660-3397
    DOI 10.3390/md9122643
    Database MEDical Literature Analysis and Retrieval System OnLINE

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