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  1. Article: Orthobunyaviruses: From Virus Binding to Penetration into Mammalian Host Cells

    Windhaber, Stefan / Xin, Qilin / Lozach, Pierre-Yves

    Viruses. 2021 May 10, v. 13, no. 5

    2021  

    Abstract: With over 80 members worldwide, Orthobunyavirus is the largest genus in the Peribunyaviridae family. Orthobunyaviruses (OBVs) are arthropod-borne viruses that are structurally simple, with a trisegmented, negative-sense RNA genome and only four ... ...

    Abstract With over 80 members worldwide, Orthobunyavirus is the largest genus in the Peribunyaviridae family. Orthobunyaviruses (OBVs) are arthropod-borne viruses that are structurally simple, with a trisegmented, negative-sense RNA genome and only four structural proteins. OBVs are potential agents of emerging and re-emerging diseases and overall represent a global threat to both public and veterinary health. The focus of this review is on the very first steps of OBV infection in mammalian hosts, from virus binding to penetration and release of the viral genome into the cytosol. Here, we address the most current knowledge and advances regarding OBV receptors, endocytosis, and fusion.
    Keywords Orthobunyavirus ; RNA ; animal health ; cytosol ; endocytosis ; mammals ; viral genome
    Language English
    Dates of publication 2021-0510
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v13050872
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Orthobunyaviruses: From Virus Binding to Penetration into Mammalian Host Cells.

    Windhaber, Stefan / Xin, Qilin / Lozach, Pierre-Yves

    Viruses

    2021  Volume 13, Issue 5

    Abstract: With over 80 members worldwide, ...

    Abstract With over 80 members worldwide,
    MeSH term(s) Animals ; Biological Transport ; Bunyaviridae Infections/virology ; Cell Membrane/metabolism ; Genome, Viral ; Host-Pathogen Interactions ; Humans ; Orthobunyavirus/physiology ; Species Specificity ; Viral Tropism ; Virion ; Virus Attachment ; Virus Internalization
    Language English
    Publishing date 2021-05-10
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v13050872
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Tecovirimat for the treatment of severe Mpox in Germany.

    Hermanussen, Lennart / Brehm, Thomas Theo / Wolf, Timo / Boesecke, Christoph / Schlabe, Stefan / Borgans, Frauke / Monin, Malte B / Jensen, Björn-Erik Ole / Windhaber, Stefan / Scholten, Stefan / Jordan, Sabine / Lütgehetmann, Marc / Wiesch, Julian Schulze Zur / Addo, Marylyn M / Mikolajewska, Agata / Niebank, Michaela / Schmiedel, Stefan

    Infection

    2023  Volume 51, Issue 5, Page(s) 1563–1568

    Abstract: Background: In May 2022, a multi-national mpox outbreak was reported in several non-endemic countries. The only licensed treatment for mpox in the European Union is the orally available small molecule tecovirimat, which in Orthopox viruses inhibits the ... ...

    Abstract Background: In May 2022, a multi-national mpox outbreak was reported in several non-endemic countries. The only licensed treatment for mpox in the European Union is the orally available small molecule tecovirimat, which in Orthopox viruses inhibits the function of a major envelope protein required for the production of extracellular virus.
    Methods: We identified presumably all patients with mpox that were treated with tecovirimat in Germany between the onset of the outbreak in May 2022 and March 2023 and obtained demographic and clinical characteristics by standardized case report forms.
    Results: A total of twelve patients with mpox were treated with tecovirimat in Germany in the study period. All but one patient identified as men who have sex with men (MSM) who were most likely infected with mpox virus (MPXV) through sexual contact. Eight of them were people living with HIV (PLWH), one of whom was newly diagnosed with HIV at the time of mpox, and four had CD4+ counts below 200/µl. Criteria for treatment with tecovirimat included severe immunosuppression, severe generalized and/or protracted symptoms, a high or increasing number of lesions, and the type and location of lesions (e.g., facial or oral soft tissue involvement, imminent epiglottitis, or tonsillar swelling). Patients were treated with tecovirimat for between six and 28 days. Therapy was generally well-tolerated, and all patients showed clinical resolution.
    Conclusions: In this cohort of twelve patients with severe mpox, treatment with tecovirimat was well tolerated and all individuals showed clinical improvement.
    MeSH term(s) Male ; Humans ; Homosexuality, Male ; Mpox (monkeypox) ; Sexual and Gender Minorities ; Germany/epidemiology ; Benzamides ; HIV Infections
    Chemical Substances Benzamides
    Language English
    Publishing date 2023-06-05
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 185104-4
    ISSN 1439-0973 ; 0300-8126 ; 0173-2129
    ISSN (online) 1439-0973
    ISSN 0300-8126 ; 0173-2129
    DOI 10.1007/s15010-023-02049-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 881: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 528: Show issues

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  4. Article ; Online: The Orthobunyavirus Germiston Enters Host Cells from Late Endosomes.

    Windhaber, Stefan / Xin, Qilin / Uckeley, Zina M / Koch, Jana / Obr, Martin / Garnier, Céline / Luengo-Guyonnot, Catherine / Duboeuf, Maëva / Schur, Florian K M / Lozach, Pierre-Yves

    Journal of virology

    2022  Volume 96, Issue 5, Page(s) e0214621

    Abstract: With more than 80 members worldwide, ... ...

    Abstract With more than 80 members worldwide, the
    MeSH term(s) Animals ; Cryoelectron Microscopy ; Endosomes/virology ; Mammals ; Orthobunyavirus/physiology ; Virus Internalization
    Language English
    Publishing date 2022-01-12
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80174-4
    ISSN 1098-5514 ; 0022-538X
    ISSN (online) 1098-5514
    ISSN 0022-538X
    DOI 10.1128/jvi.02146-21
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 609: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article: Novel Toscana Virus Reverse Genetics System Establishes NSs as an Antagonist of Type I Interferon Responses

    Woelfl, Franziska / Léger, Psylvia / Oreshkova, Nadia / Pahmeier, Felix / Windhaber, Stefan / Koch, Jana / Stanifer, Megan / Roman Sosa, Gleyder / Uckeley, Zina M / Rey, Felix A / Boulant, Steeve / Kortekaas, Jeroen / Wichgers Schreur, Paul J / Lozach, Pierre-Yves

    Viruses. 2020 Apr. 04, v. 12, no. 4

    2020  

    Abstract: The sand fly-borne Toscana virus (TOSV) is the major cause of human meningoencephalitis in the Mediterranean basin during the summer season. In this work, we have developed a T7 RNA polymerase-driven reverse genetics system to recover infectious ... ...

    Abstract The sand fly-borne Toscana virus (TOSV) is the major cause of human meningoencephalitis in the Mediterranean basin during the summer season. In this work, we have developed a T7 RNA polymerase-driven reverse genetics system to recover infectious particles of a lineage B strain of TOSV. The viral protein pattern and growth properties of the rescued virus (rTOSV) were found to be similar to those of the corresponding wild-type (wt) virus. Using this system, we genetically engineered a TOSV mutant lacking expression of the non-structural protein NSs (rTOSVɸNSs). Unlike rTOSV and the wt virus, rTOSVɸNSs was unable to (i) suppress interferon (IFN)-b messenger RNA induction; and (ii) grow efficiently in cells producing IFN-b. Together, our results highlight the importance of NSs for TOSV in evading the IFN response and provide a comprehensive toolbox to investigate the TOSV life cycle in mammalian and insect host cells, including several novel polyclonal antibodies.
    Keywords Sandfly fever Naples phlebovirus ; antagonists ; genetic engineering ; human diseases ; insects ; interferons ; mammals ; meningoencephalitis ; messenger RNA ; mutants ; polyclonal antibodies ; reverse genetics ; viral nonstructural proteins ; viruses
    Language English
    Dates of publication 2020-0404
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v12040400
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Novel Toscana Virus Reverse Genetics System Establishes NSs as an Antagonist of Type I Interferon Responses.

    Woelfl, Franziska / Léger, Psylvia / Oreshkova, Nadia / Pahmeier, Felix / Windhaber, Stefan / Koch, Jana / Stanifer, Megan / Roman Sosa, Gleyder / Uckeley, Zina M / Rey, Felix A / Boulant, Steeve / Kortekaas, Jeroen / Wichgers Schreur, Paul J / Lozach, Pierre-Yves

    Viruses

    2020  Volume 12, Issue 4

    Abstract: The sand fly-borne Toscana virus (TOSV) is the major cause of human meningoencephalitis in the Mediterranean basin during the summer season. In this work, we have developed a T7 RNA polymerase-driven reverse genetics system to recover infectious ... ...

    Abstract The sand fly-borne Toscana virus (TOSV) is the major cause of human meningoencephalitis in the Mediterranean basin during the summer season. In this work, we have developed a T7 RNA polymerase-driven reverse genetics system to recover infectious particles of a lineage B strain of TOSV. The viral protein pattern and growth properties of the rescued virus (rTOSV) were found to be similar to those of the corresponding wild-type (wt) virus. Using this system, we genetically engineered a TOSV mutant lacking expression of the non-structural protein NSs (rTOSVɸNSs). Unlike rTOSV and the wt virus, rTOSVɸNSs was unable to (i) suppress interferon (IFN)-b messenger RNA induction; and (ii) grow efficiently in cells producing IFN-b. Together, our results highlight the importance of NSs for TOSV in evading the IFN response and provide a comprehensive toolbox to investigate the TOSV life cycle in mammalian and insect host cells, including several novel polyclonal antibodies.
    MeSH term(s) A549 Cells ; Animals ; Antibodies, Viral/immunology ; Cell Line ; Chlorocebus aethiops ; Cricetinae ; DNA-Directed RNA Polymerases/genetics ; Genome, Viral ; Humans ; Insecta ; Interferon-beta/antagonists & inhibitors ; Interferon-beta/immunology ; Kidney/cytology ; Mutation ; Reverse Genetics ; Sandfly fever Naples virus/genetics ; Sandfly fever Naples virus/immunology ; Vero Cells ; Viral Nonstructural Proteins/genetics ; Viral Nonstructural Proteins/immunology ; Viral Proteins/genetics
    Chemical Substances Antibodies, Viral ; Viral Nonstructural Proteins ; Viral Proteins ; Interferon-beta (77238-31-4) ; bacteriophage T7 RNA polymerase (EC 2.7.7.-) ; DNA-Directed RNA Polymerases (EC 2.7.7.6)
    Language English
    Publishing date 2020-04-04
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v12040400
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Novel toscana virus reverse genetics system establishes NSS as an antagonist of type I interferon responses

    Woelfl, Franziska / Léger, Psylvia / Oreshkova, Nadia / Pahmeier, Felix / Windhaber, Stefan / Koch, Jana / Stanifer, Megan / Sosa, Gleyder Roman / Uckeley, Zina M. / Rey, Felix A. / Boulant, Steeve / Kortekaas, Jeroen / Wichgers Schreur, Paul J. / Lozach, Pierre Yves

    Viruses

    2020  Volume 12, Issue 4

    Abstract: The sand fly-borne Toscana virus (TOSV) is the major cause of human meningoencephalitis in the Mediterranean basin during the summer season. In this work, we have developed a T7 RNA polymerase-driven reverse genetics system to recover infectious ... ...

    Abstract The sand fly-borne Toscana virus (TOSV) is the major cause of human meningoencephalitis in the Mediterranean basin during the summer season. In this work, we have developed a T7 RNA polymerase-driven reverse genetics system to recover infectious particles of a lineage B strain of TOSV. The viral protein pattern and growth properties of the rescued virus (rTOSV) were found to be similar to those of the corresponding wild-type (wt) virus. Using this system, we genetically engineered a TOSV mutant lacking expression of the non-structural protein NSs (rTOSVϕNSs). Unlike rTOSV and the wt virus, rTOSVϕNSs was unable to (i) suppress interferon (IFN)-b messenger RNA induction; and (ii) grow efficiently in cells producing IFN-b. Together, our results highlight the importance of NSs for TOSV in evading the IFN response and provide a comprehensive toolbox to investigate the TOSV life cycle in mammalian and insect host cells, including several novel polyclonal antibodies.
    Keywords Arbovirus ; Bunyavirales ; Interferon ; Neglected diseases ; Phenuiviridae ; Phlebovirus ; Reverse genetics ; Sand fly fever ; Toscana virus
    Subject code 570
    Language English
    Publishing country nl
    Document type Article ; Online
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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