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  1. AU="Wohlfert, Elizabeth A"
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  1. Article ; Online: In Vitro Analyses of T Cell Effector Differentiation.

    Wohlfert, Elizabeth A / Carpenter, Andrea C / Belkaid, Yasmine / Bosselut, Rémy

    Methods in molecular biology (Clifton, N.J.)

    2020  Volume 1323, Page(s) 117–128

    Abstract: In vitro culture is an important complement, or substitute, to in vivo approaches in order to study T cell effector differentiation. Here, we describe culture conditions that generate specific effector cell types by exposing naïve T cells to appropriate ... ...

    Abstract In vitro culture is an important complement, or substitute, to in vivo approaches in order to study T cell effector differentiation. Here, we describe culture conditions that generate specific effector cell types by exposing naïve T cells to appropriate cytokine signals.
    MeSH term(s) Animals ; Antigen-Presenting Cells/cytology ; Antigen-Presenting Cells/metabolism ; Cell Differentiation ; Cell Separation ; Dendritic Cells/cytology ; Dendritic Cells/metabolism ; Flow Cytometry ; Fluorescent Antibody Technique ; Immunophenotyping ; In Vitro Techniques ; Mice ; Spleen/cytology ; T-Lymphocyte Subsets/cytology ; T-Lymphocyte Subsets/metabolism
    Language English
    Publishing date 2020-01-09
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-2809-5_10
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Tbet Expression by Regulatory T Cells Is Needed to Protect against Th1-Mediated Immunopathology during

    Warunek, Jordan / Jin, Richard M / Blair, Sarah J / Garis, Matthew / Marzullo, Brandon / Wohlfert, Elizabeth A

    ImmunoHorizons

    2021  Volume 5, Issue 12, Page(s) 931–943

    Abstract: Toxoplasma ... ...

    Abstract Toxoplasma gondii
    MeSH term(s) Animals ; Female ; Forkhead Transcription Factors/genetics ; Forkhead Transcription Factors/metabolism ; Interferon-gamma/metabolism ; Lymphocyte Activation ; Male ; Mice ; T-Box Domain Proteins/genetics ; T-Box Domain Proteins/metabolism ; T-Lymphocytes, Regulatory/immunology ; Toxoplasmosis/immunology ; Toxoplasmosis/metabolism ; Toxoplasmosis/pathology ; Tumor Necrosis Factor-alpha/metabolism
    Chemical Substances Forkhead Transcription Factors ; Foxp3 protein, mouse ; T-Box Domain Proteins ; T-box transcription factor TBX21 ; Tumor Necrosis Factor-alpha ; Interferon-gamma (82115-62-6)
    Language English
    Publishing date 2021-12-10
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ISSN 2573-7732
    ISSN (online) 2573-7732
    DOI 10.4049/immunohorizons.2100080
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Cryptococcus neoformans Coinfection Dampens the TNF-α Response in HIV-1-Infected Human THP-1 Macrophages.

    Kalem, Murat C / Humby, Monica S / Wohlfert, Elizabeth A / Jacobs, Amy / Panepinto, John C

    mSphere

    2021  Volume 6, Issue 2

    Abstract: Cryptococcus ... ...

    Abstract Cryptococcus neoformans
    MeSH term(s) Coinfection/immunology ; Coinfection/microbiology ; Coinfection/virology ; Cryptococcus neoformans/immunology ; Cryptococcus neoformans/pathogenicity ; HIV-1/immunology ; HIV-1/pathogenicity ; Humans ; Lung ; Macrophages/immunology ; Macrophages/virology ; Protein Serine-Threonine Kinases/metabolism ; Signal Transduction/immunology ; THP-1 Cells ; Tumor Necrosis Factor-alpha/analysis ; Tumor Necrosis Factor-alpha/genetics ; Tumor Necrosis Factor-alpha/immunology ; Up-Regulation ; NF-kappaB-Inducing Kinase
    Chemical Substances Tumor Necrosis Factor-alpha ; Protein Serine-Threonine Kinases (EC 2.7.11.1)
    Language English
    Publishing date 2021-03-24
    Publishing country United States
    Document type Journal Article
    ISSN 2379-5042
    ISSN (online) 2379-5042
    DOI 10.1128/mSphere.00213-21
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Chronic infection stunts macrophage heterogeneity and disrupts immune-mediated myogenesis.

    Jin, Richard M / Warunek, Jordan / Wohlfert, Elizabeth A

    JCI insight

    2018  Volume 3, Issue 18

    Abstract: The robust regenerative potential of skeletal muscle is imperative for the maintenance of tissue function across a host of potential insults including exercise, infection, and trauma. The highly coordinated action of multiple immune populations, ... ...

    Abstract The robust regenerative potential of skeletal muscle is imperative for the maintenance of tissue function across a host of potential insults including exercise, infection, and trauma. The highly coordinated action of multiple immune populations, especially macrophages, plays an indispensable role in guiding this reparative program. However, it remains unclear how skeletal muscle repair proceeds in a chronically inflamed setting, such as infection, where an active immune response is already engaged. To address this question, we used a cardiotoxin injury model to challenge the reparative potential of chronically infected muscle. Compared with regenerating naive skeletal muscle, infected skeletal muscle exhibited multiple indicators of delayed muscle repair including a divergent morphologic response to injury and dysregulated expression of myogenic regulatory factors. Further, using both flow cytometric and single-cell RNA sequencing approaches, we show that reduced macrophage heterogeneity due to delayed emergence of restorative subsets underlies dysfunctional tissue repair during chronic infection. Our findings highlight how the preexisting inflammatory environment within tissue alters reparative immunity and ultimately the quality of tissue regeneration.
    MeSH term(s) Animals ; Chronic Disease ; Communicable Diseases/immunology ; Computational Biology ; Disease Models, Animal ; Female ; Gene Expression Profiling ; Macrophages/metabolism ; Mice ; Mice, Inbred C57BL ; Muscle Development/genetics ; Muscle Development/immunology ; Muscle Development/physiology ; Muscle, Skeletal/injuries ; Muscle, Skeletal/metabolism ; Muscle, Skeletal/pathology ; Myogenic Regulatory Factors/genetics ; Myogenic Regulatory Factors/metabolism ; Toxoplasma ; Toxoplasmosis
    Chemical Substances Myogenic Regulatory Factors
    Language English
    Publishing date 2018-09-20
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ISSN 2379-3708
    ISSN (online) 2379-3708
    DOI 10.1172/jci.insight.121549
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Therapeutic administration of IL-10 and amphiregulin alleviates chronic skeletal muscle inflammation and damage induced by infection.

    Jin, Richard M / Warunek, Jordan / Wohlfert, Elizabeth A

    ImmunoHorizons

    2018  Volume 2, Issue 5, Page(s) 142–154

    Abstract: Maintenance of tissue integrity in skeletal muscle requires the immunomodulatory and regenerative functions of muscle-resident regulatory T cells (Tregs). Chronic skeletal muscle infections, such as ... ...

    Abstract Maintenance of tissue integrity in skeletal muscle requires the immunomodulatory and regenerative functions of muscle-resident regulatory T cells (Tregs). Chronic skeletal muscle infections, such as with
    Language English
    Publishing date 2018-11-02
    Publishing country United States
    Document type Journal Article
    ISSN 2573-7732
    ISSN 2573-7732
    DOI 10.4049/immunohorizons.1800024
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Neutrophils Are Required During Immunization With the Pneumococcal Conjugate Vaccine for Protective Antibody Responses and Host Defense Against Infection.

    Tchalla, Essi Y I / Bhalla, Manmeet / Wohlfert, Elizabeth A / Bou Ghanem, Elsa N

    The Journal of infectious diseases

    2020  Volume 222, Issue 8, Page(s) 1363–1370

    Abstract: Neutrophils can shape adaptive immunity; however, their role in vaccine-induced protection against infections in vivo remains unclear. Here, we tested their role in the clinically relevant polysaccharide conjugate vaccine against Streptococcus pneumoniae ...

    Abstract Neutrophils can shape adaptive immunity; however, their role in vaccine-induced protection against infections in vivo remains unclear. Here, we tested their role in the clinically relevant polysaccharide conjugate vaccine against Streptococcus pneumoniae (pneumococcus). We antibody depleted neutrophils during vaccination, allowed them to recover, and 4 weeks later challenged mice with pneumococci. We found that while isotype-treated vaccinated controls were protected against an otherwise lethal infection in naive mice, full protection was lost upon neutrophil depletion. Compared to vaccinated controls, neutrophil-depleted mice had higher lung bacterial burdens, increased incidence of bacteremia, and lower survival rates. Sera from neutrophil-depleted mice had less antipneumococcal IgG2c and IgG3, were less efficient at inducing opsonophagocytic killing of bacteria by neutrophils in vitro, and were worse at protecting naive mice against pneumococcal pneumonia. In summary, neutrophils are required during vaccination for optimal host protection, which has important implications for future vaccine design against pneumococci and other pathogens.
    MeSH term(s) Animals ; Antibodies, Bacterial/immunology ; Antibody Formation ; Bacterial Load ; Female ; Immunization ; Immunoglobulin Class Switching ; Mice ; Mice, Inbred C57BL ; Neutrophils/immunology ; Pneumococcal Infections/immunology ; Pneumococcal Infections/microbiology ; Pneumococcal Infections/prevention & control ; Pneumococcal Vaccines/administration & dosage ; Pneumococcal Vaccines/immunology ; Streptococcus pneumoniae/immunology ; Vaccines, Conjugate/administration & dosage ; Vaccines, Conjugate/immunology
    Chemical Substances Antibodies, Bacterial ; Pneumococcal Vaccines ; Vaccines, Conjugate
    Language English
    Publishing date 2020-04-24
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 3019-3
    ISSN 1537-6613 ; 0022-1899
    ISSN (online) 1537-6613
    ISSN 0022-1899
    DOI 10.1093/infdis/jiaa242
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.MO 445: Show issues

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  7. Article ; Online: Brains and Brawn: Toxoplasma Infections of the Central Nervous System and Skeletal Muscle.

    Wohlfert, Elizabeth A / Blader, Ira J / Wilson, Emma H

    Trends in parasitology

    2017  Volume 33, Issue 7, Page(s) 519–531

    Abstract: Toxoplasma gondii is a widespread parasitic pathogen that infects over a third of the world's population. Following an acute infection, the parasite can persist within its mammalian host as intraneuronal or intramuscular cysts. Cysts will occasionally ... ...

    Abstract Toxoplasma gondii is a widespread parasitic pathogen that infects over a third of the world's population. Following an acute infection, the parasite can persist within its mammalian host as intraneuronal or intramuscular cysts. Cysts will occasionally reactivate, and - depending on the host's immune status and site of reactivation - encephalitis or myositis can develop. Because these diseases have high levels of morbidity and can be lethal, it is important to understand how Toxoplasma traffics to these tissues, how the immune response controls parasite burden and contributes to tissue damage, and what mechanisms underlie neurological and muscular pathologies that toxoplasmosis patients present with. This review aims to summarize recent important developments addressing these critical topics.
    MeSH term(s) Animals ; Central Nervous System/parasitology ; Central Nervous System/pathology ; Humans ; Muscle, Skeletal/parasitology ; Muscle, Skeletal/pathology ; Toxoplasmosis/immunology ; Toxoplasmosis/parasitology
    Language English
    Publishing date 2017-05-05
    Publishing country England
    Document type Journal Article ; Review ; Research Support, N.I.H., Extramural
    ZDB-ID 2036227-4
    ISSN 1471-5007 ; 1471-4922
    ISSN (online) 1471-5007
    ISSN 1471-4922
    DOI 10.1016/j.pt.2017.04.001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.B 2898: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
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    Zs.MG 16: Show issues
    Zs.MO 251: Show issues

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  8. Article: Brains and Brawn: Toxoplasma Infections of the Central Nervous System and Skeletal Muscle

    Wohlfert, Elizabeth A / Emma H. Wilson / Ira J. Blader

    Trends in parasitology. 2017 July, v. 33, no. 7

    2017  

    Abstract: Toxoplasma gondii is a widespread parasitic pathogen that infects over a third of the world’s population. Following an acute infection, the parasite can persist within its mammalian host as intraneuronal or intramuscular cysts. Cysts will occasionally ... ...

    Abstract Toxoplasma gondii is a widespread parasitic pathogen that infects over a third of the world’s population. Following an acute infection, the parasite can persist within its mammalian host as intraneuronal or intramuscular cysts. Cysts will occasionally reactivate, and – depending on the host’s immune status and site of reactivation – encephalitis or myositis can develop. Because these diseases have high levels of morbidity and can be lethal, it is important to understand how Toxoplasma traffics to these tissues, how the immune response controls parasite burden and contributes to tissue damage, and what mechanisms underlie neurological and muscular pathologies that toxoplasmosis patients present with. This review aims to summarize recent important developments addressing these critical topics.
    Keywords acute course ; central nervous system ; encephalitis ; immune response ; infectious diseases ; mammals ; morbidity ; myositis ; parasites ; pathogens ; patients ; skeletal muscle ; Toxoplasma gondii ; toxoplasmosis
    Language English
    Dates of publication 2017-07
    Size p. 519-531.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 2036227-4
    ISSN 1471-5007 ; 1471-4922
    ISSN (online) 1471-5007
    ISSN 1471-4922
    DOI 10.1016/j.pt.2017.04.001
    Database NAL-Catalogue (AGRICOLA)

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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
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  9. Article ; Online: Rational design of a nanoparticle platform for oral prophylactic immunotherapy to prevent immunogenicity of therapeutic proteins.

    Nguyen, Nhan H / Glassman, Fiona Y / Dingman, Robert K / Shenoy, Gautam N / Wohlfert, Elizabeth A / Kay, Jason G / Bankert, Richard B / Balu-Iyer, Sathy V

    Scientific reports

    2021  Volume 11, Issue 1, Page(s) 17853

    Abstract: The safety and efficacy of several life-saving therapeutic proteins are compromised due to their immunogenicity. Once a sustained immune response against a protein-based therapy is established, clinical options that are safe and cost-effective become ... ...

    Abstract The safety and efficacy of several life-saving therapeutic proteins are compromised due to their immunogenicity. Once a sustained immune response against a protein-based therapy is established, clinical options that are safe and cost-effective become limited. Prevention of immunogenicity of therapeutic proteins prior to their initial use is critical as it is often difficult to reverse an established immune response. Here, we discuss a rational design and testing of a phosphatidylserine-containing nanoparticle platform for novel oral prophylactic reverse vaccination approach, i.e., pre-treatment of a therapeutic protein in the presence of nanoparticles to prevent immunogenicity of protein therapies.
    MeSH term(s) Animals ; Immunotherapy ; Mice ; Nanoparticles
    Language English
    Publishing date 2021-09-08
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-021-97333-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: The

    Glausen, Taylor G / Carrillo, Gabriela L / Jin, Richard M / Boyle, Jon P / Saeij, Jeroen P J / Wohlfert, Elizabeth A / Fox, Michael A / Blader, Ira J

    mBio

    2021  Volume 12, Issue 3, Page(s) e0133121

    Abstract: Toxoplasmic encephalitis can develop in individuals infected with the protozoan parasite Toxoplasma gondii and is typified by parasite replication and inflammation within the brain. Patients often present with seizures, but the parasite genes and host ... ...

    Abstract Toxoplasmic encephalitis can develop in individuals infected with the protozoan parasite Toxoplasma gondii and is typified by parasite replication and inflammation within the brain. Patients often present with seizures, but the parasite genes and host pathways involved in seizure development and/or propagation are unknown. We previously reported that seizure induction in
    MeSH term(s) Animals ; Brain/immunology ; Brain/parasitology ; Brain/pathology ; Female ; Gene Expression ; Genome, Protozoan ; Host-Parasite Interactions/immunology ; Humans ; Interleukin-1beta/genetics ; Interleukin-1beta/metabolism ; Mice ; Mice, Inbred C57BL ; Protozoan Proteins/genetics ; Protozoan Proteins/immunology ; Seizures/immunology ; Seizures/parasitology ; Signal Transduction/immunology ; Toxoplasma/genetics ; Toxoplasma/immunology ; Toxoplasmosis, Animal/immunology ; Toxoplasmosis, Animal/parasitology ; Virulence Factors
    Chemical Substances Interleukin-1beta ; Protozoan Proteins ; Virulence Factors
    Language English
    Publishing date 2021-06-22
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2557172-2
    ISSN 2150-7511 ; 2161-2129
    ISSN (online) 2150-7511
    ISSN 2161-2129
    DOI 10.1128/mBio.01331-21
    Database MEDical Literature Analysis and Retrieval System OnLINE

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