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  1. Article ; Online: Detection of Novel Human Astrovirus MLB1 by a Commercial Viral Gastroenteritis Multiplex Assay.

    Ho, Yolanda I I / Lee, Christine W S / Wong, Ann H

    Microbiology spectrum

    2022  Volume 10, Issue 3, Page(s) e0046922

    MeSH term(s) Enterovirus Infections ; Feces ; Gastroenteritis/diagnosis ; Humans ; Infant ; Mamastrovirus/genetics
    Language English
    Publishing date 2022-05-23
    Publishing country United States
    Document type Letter
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.00469-22
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  2. Article ; Online: Comparison of PneumID real-time PCR assay with Amplex eazyplex LAMP assay for laboratory diagnosis of Pneumocystis jirovecii Pneumonia.

    Ng, Willy W Y / Ho, Yolanda I I / Wong, Ann H / Leung, Eddie C M / Lee, Alfred L H / Chow, Viola C Y

    Medical mycology

    2022  

    Abstract: We compared PneumID PCR with Amplex eazyplex LAMP assay for the diagnosis of Pneumocystis jirovecii pneumonia (PJP). Both assays enable accurate diagnosis of definite PJP. Cut-off cycle threshold of the PneumID assay was < 26.68 while the cut-off time-to- ...

    Abstract We compared PneumID PCR with Amplex eazyplex LAMP assay for the diagnosis of Pneumocystis jirovecii pneumonia (PJP). Both assays enable accurate diagnosis of definite PJP. Cut-off cycle threshold of the PneumID assay was < 26.68 while the cut-off time-to-positivity of the eazyplex assay was 16:02 (minutes:seconds). The positive and negative percentage agreement of eazyplex assay with PneumID assay was 75.0% and 100.0% respectively, while the overall agreement was substantial with kappa = 0.80. For both assays, establishment of cut-off values to differentiate probable PJP from colonization was not feasible as results overlapped.
    Language English
    Publishing date 2022-06-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 1421796-x
    ISSN 1460-2709 ; 1369-3786
    ISSN (online) 1460-2709
    ISSN 1369-3786
    DOI 10.1093/mmy/myac043
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  3. Article ; Online: Comparison of Captia

    Wong, Ann H / Ho, Yolanda I I / Tang, Kevin P S / Lai, Raymond W M

    Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

    2020  Volume 127, Page(s) 104342

    Abstract: Background: Correlation of the assay cut-off values for Captia: Objective: The aim of this study was to compare the relative performance of the Captia: Study design: Correlation of the cut-off value of both assays with the immunoprotective level ... ...

    Abstract Background: Correlation of the assay cut-off values for Captia
    Objective: The aim of this study was to compare the relative performance of the Captia
    Study design: Correlation of the cut-off value of both assays with the immunoprotective level was determined with the 3rd WHO Measles IgG International Standard. One hundred clinical samples including frozen and fresh were tested with both assays. The positive percentage agreement (PPA) based on the manufacturers' interpretation and the WHO recommended immunoprotective level was compared.
    Results: Samples tested positive by the Captia
    Conclusions: Captia
    MeSH term(s) Antibodies, Viral/blood ; Humans ; Immunoassay/methods ; Immunoglobulin G/blood ; Measles/diagnosis ; Measles/immunology ; Measles virus/immunology ; Reagent Kits, Diagnostic ; Sensitivity and Specificity ; World Health Organization
    Chemical Substances Antibodies, Viral ; Immunoglobulin G ; Reagent Kits, Diagnostic
    Language English
    Publishing date 2020-04-06
    Publishing country Netherlands
    Document type Comparative Study ; Journal Article
    ZDB-ID 1446080-4
    ISSN 1873-5967 ; 1386-6532
    ISSN (online) 1873-5967
    ISSN 1386-6532
    DOI 10.1016/j.jcv.2020.104342
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  4. Article ; Online: Comparison of the Cepheid Xpert Xpress Flu/RSV Assay to in-house Flu/RSV triplex real-time RT-PCR for rapid molecular detection of Influenza A, Influenza B and Respiratory Syncytial Virus in respiratory specimens.

    Ho, Yolanda I I / Wong, Ann H / Lai, Raymond W M

    Journal of medical microbiology

    2018  Volume 67, Issue 11, Page(s) 1576–1580

    Abstract: This study compared the performance of the commercially available Xpert Xpress Flu/RSV assay to an in-house FluAB/RSV triplex real-time RT-PCR assay for the detection of influenza A/B viruses and respiratory syncitial virus (RSV) from both nasopharyngeal ...

    Abstract This study compared the performance of the commercially available Xpert Xpress Flu/RSV assay to an in-house FluAB/RSV triplex real-time RT-PCR assay for the detection of influenza A/B viruses and respiratory syncitial virus (RSV) from both nasopharyngeal aspirate (NPA) and nasopharyngeal flocked swab (NPS). A total of 20 external quality assurance (EQA) samples and 172 clinical respiratory samples were tested prospectively using both the Xpert Xpress Flu/RSV assay and the in-house FluAB/RSV triplex assay. For the EQA samples, concordance rate was 100 % when tested with both assays. For clinical samples, there was 100 % agreement between the two assays for detection of influenza A and influenza B, 96.7 % agreement for detection of RSV and 99.7 % agreement for negative results. With a shortened turnaround time and good diagnostic performance, application of the Xpert Xpress Flu/RSV assay can facilitate patient triage for prompt implementation of infection control measures and management of high-risk patients during influenza epidemics.
    MeSH term(s) Biological Assay ; DNA Primers/genetics ; Humans ; Influenza A virus/genetics ; Influenza A virus/isolation & purification ; Influenza B virus/genetics ; Influenza B virus/isolation & purification ; Influenza, Human/diagnosis ; Influenza, Human/virology ; Molecular Diagnostic Techniques/instrumentation ; Molecular Diagnostic Techniques/methods ; Multiplex Polymerase Chain Reaction/methods ; Nasopharynx/virology ; Real-Time Polymerase Chain Reaction/instrumentation ; Real-Time Polymerase Chain Reaction/methods ; Respiratory Syncytial Virus Infections/diagnosis ; Respiratory Syncytial Virus Infections/virology ; Respiratory Syncytial Viruses/genetics ; Respiratory Syncytial Viruses/isolation & purification ; Sensitivity and Specificity
    Chemical Substances DNA Primers
    Language English
    Publishing date 2018-09-12
    Publishing country England
    Document type Comparative Study ; Journal Article
    ZDB-ID 218356-0
    ISSN 1473-5644 ; 0022-2615
    ISSN (online) 1473-5644
    ISSN 0022-2615
    DOI 10.1099/jmm.0.000841
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    Zs.A 634: Show issues Location:
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  5. Article ; Online: Performance evaluation of the re-standardized Abbott Architect anti-HBs assay.

    Wong, Ann H / Ho, Yolanda I I / Lai, Raymond W M

    Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology

    2018  Volume 111, Page(s) 1–3

    Abstract: Background: As defined by World Health Organization (WHO), an antibody level of ≥ 10mIU/mL to hepatitis B virus confers protection. With the launching of Abbott anti-HBs assay re-standardized to the 2: Objectives: To evaluate the performance of the ... ...

    Abstract Background: As defined by World Health Organization (WHO), an antibody level of ≥ 10mIU/mL to hepatitis B virus confers protection. With the launching of Abbott anti-HBs assay re-standardized to the 2
    Objectives: To evaluate the performance of the re-standardized Abbott Architect anti-HBs assay and to determine the impact of the upward shift.
    Study design: A total of 52 samples, including 12 external quality assurance programme samples and 40 clinical samples were tested with both the Abbott 1
    Results: Verification of the re-standardized assay with the 2
    Conclusions: Final interpretation of immune status to hepatitis B was not affected by the upward shift following introduction of the new Abbott anti-HBs assay except for previously negative samples with anti-HBs levels between >5.00 to <10.00 mIU/mL.
    MeSH term(s) Hepatitis B/diagnosis ; Hepatitis B Antibodies/blood ; Hepatitis B virus/immunology ; Humans ; Reagent Kits, Diagnostic/standards ; Reference Standards ; Serologic Tests/standards ; World Health Organization
    Chemical Substances Hepatitis B Antibodies ; Reagent Kits, Diagnostic
    Language English
    Publishing date 2018-12-15
    Publishing country Netherlands
    Document type Evaluation Study ; Journal Article
    ZDB-ID 1446080-4
    ISSN 1873-5967 ; 1386-6532
    ISSN (online) 1873-5967
    ISSN 1386-6532
    DOI 10.1016/j.jcv.2018.12.003
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  6. Article ; Online: Comparison of three commercial SARS-CoV-2 assays for pooled testing of deep throat saliva for surveillance of patients attending general outpatient clinics.

    Ho, Yolanda I / Wong, Ann H / Tang, Kevin P S / Wong, River C W / Leung, Eddie C M / Lai, Raymond W M

    Journal of medical virology

    2021  Volume 93, Issue 4, Page(s) 1917–1919

    MeSH term(s) COVID-19/diagnosis ; COVID-19 Nucleic Acid Testing ; Humans ; Nasopharynx/virology ; SARS-CoV-2/isolation & purification ; Saliva/virology ; Specimen Handling
    Language English
    Publishing date 2021-01-11
    Publishing country United States
    Document type Comparative Study ; Letter
    ZDB-ID 752392-0
    ISSN 1096-9071 ; 0146-6615
    ISSN (online) 1096-9071
    ISSN 0146-6615
    DOI 10.1002/jmv.26764
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    Zs.A 1320: Show issues Location:
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  7. Article ; Online: Rapid adaptation and continuous performance evaluation of SARS-CoV-2 envelope gene (E-gene) real-time RT-PCR assays to support the hospital surge in test demand.

    Ho, Yolanda I I / Wong, Ann H / Leung, Eddie C M / Wong, River C W / Lai, Raymond W M

    Journal of medical virology

    2020  Volume 93, Issue 3, Page(s) 1824–1827

    Abstract: We describe the timely adaption of both published WHO E-gene protocol and commercially available LightMix Modular E-gene assay to the test platform (ABI 7900 Fast real-time analyzer and TaqMan Fast One-step Virus Master Mix) available in an accredited ... ...

    Abstract We describe the timely adaption of both published WHO E-gene protocol and commercially available LightMix Modular E-gene assay to the test platform (ABI 7900 Fast real-time analyzer and TaqMan Fast One-step Virus Master Mix) available in an accredited tertiary hospital laboratory with an on-going evaluation to ensure the provision of quality service within the time constraint. The LightMix Modular E-gene was slightly more sensitive when compared to the WHO E-gene, both analytically and diagnostically. The assay was recommended for screening of SARS-CoV-2 infection. With the availability of technically competent staff through continuous training, the provision of round-the-clock service is feasible despite the test is of high complexity. The thermal cycling duration of the adapted LightMix E-gene and WHO E-gene is shortened by half and one hour respectively and allows the number of runs to double when 24-h round-the-clock service is provided. An increase in testing capacity could support surges in testing demand, which is essential to control the current SARS-CoV-2 pandemic, to prevent potential overwhelming of the healthcare system, and to optimize utilization of the isolation beds.
    MeSH term(s) COVID-19/diagnosis ; COVID-19/virology ; COVID-19 Testing/methods ; Clinical Laboratory Techniques/methods ; Coronavirus Envelope Proteins/genetics ; Genes, env/genetics ; Hospitals ; Humans ; Pandemics/prevention & control ; RNA, Viral/genetics ; Real-Time Polymerase Chain Reaction/methods ; Reverse Transcriptase Polymerase Chain Reaction/methods ; SARS-CoV-2/genetics ; Sensitivity and Specificity
    Chemical Substances Coronavirus Envelope Proteins ; RNA, Viral ; envelope protein, SARS-CoV-2
    Keywords covid19
    Language English
    Publishing date 2020-11-22
    Publishing country United States
    Document type Journal Article
    ZDB-ID 752392-0
    ISSN 1096-9071 ; 0146-6615
    ISSN (online) 1096-9071
    ISSN 0146-6615
    DOI 10.1002/jmv.26660
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  8. Article: Rapid adaptation and continuous performance evaluation of SARS-CoV-2 envelope gene (E-gene) real-time RT-PCR assays to support the hospital surge in test demand

    Ho, Yolanda Ii / Wong, Ann H / Leung, Eddie Cm / Wong, River Cw / Lai, Raymond Wm

    J. med. virol

    Abstract: We describe timely adaption of both published WHO E-gene protocol and commercially available LightMix Modular E-gene assay to the test platform (ABI 7900 Fast real-time analyzer and TaqMan Fast One-step Virus Master Mix) available in an accredited ... ...

    Abstract We describe timely adaption of both published WHO E-gene protocol and commercially available LightMix Modular E-gene assay to the test platform (ABI 7900 Fast real-time analyzer and TaqMan Fast One-step Virus Master Mix) available in an accredited tertiary hospital laboratory with on-going evaluation to ensure provision of quality service within time constraint. The LightMix Modular E-gene was slightly more sensitive when compared to the WHO E-gene, both analytically and diagnostically. The assay was recommended for screening of SARS-CoV-2 infection. With the availability of technically competent staff through continuous training, provision of round the clock service is feasible despite the test is of high complexity. Thermal cycling duration of the adapted LightMix E-gene and WHO E-gene is shortened by half and one hour respectively and allows the number of runs to double when 24-hour round-the-clock service is provided. Increase in testing capacity could support surges in testing demand, which is essential to control the current SARS-CoV-2 pandemic, to prevent potential overwhelming of the healthcare system and to optimize utilization of the isolation beds. This article is protected by copyright. All rights reserved.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #915162
    Database COVID19

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  9. Article ; Online: Hepatitis B infection acquired after haematopioetic stem cell transplant through horizontal mode.

    Chan, Martin C W / Cheng, Frankie W T / Sze, Kin H / Wong, Ann H / Leung, Alex W K / Chan, Paul K S / Li, Chi K

    Journal of medical virology

    2017  Volume 89, Issue 10, Page(s) 1882–1884

    Abstract: A 22 month old child with thalassaemia major received unrelated umbilical cord blood transplantation. She was born to mother of HBsAg carrier and received hepatitis B immunoglobulin at birth and hepatitis B vaccination. She was HBsAg negative and anti- ... ...

    Abstract A 22 month old child with thalassaemia major received unrelated umbilical cord blood transplantation. She was born to mother of HBsAg carrier and received hepatitis B immunoglobulin at birth and hepatitis B vaccination. She was HBsAg negative and anti-HBs positive before transplantation. After transplant, she was taken care by her mother and found to be HBsAg positive at 2 year post-transplant. Genotyping of the mother's and child's HBV status confirmed to be of same genotype and demonstrated horizontal transmission in post-transplant setting. Passive immunization of HBV may be considered in early post-transplant phase to prevent horizontal transmission of HBV, and antiviral treatment of the carer should be offered to prevent transmission of infection to immunocompromised child.
    MeSH term(s) Antiviral Agents/therapeutic use ; Disease Transmission, Infectious ; Female ; Genotype ; Hepatitis B/drug therapy ; Hepatitis B/prevention & control ; Hepatitis B/transmission ; Hepatitis B Antibodies/blood ; Hepatitis B Surface Antigens/immunology ; Hepatitis B Vaccines ; Hepatitis B virus/genetics ; Humans ; Immunoglobulins/blood ; Infant ; Mothers ; Risk Factors ; Stem Cell Transplantation/adverse effects ; Vaccination
    Chemical Substances Antiviral Agents ; Hepatitis B Antibodies ; Hepatitis B Surface Antigens ; Hepatitis B Vaccines ; Immunoglobulins ; hepatitis B hyperimmune globulin (XII270YC6M)
    Language English
    Publishing date 2017-05-23
    Publishing country United States
    Document type Case Reports ; Journal Article
    ZDB-ID 752392-0
    ISSN 1096-9071 ; 0146-6615
    ISSN (online) 1096-9071
    ISSN 0146-6615
    DOI 10.1002/jmv.24835
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  10. Article ; Online: The impact of pandemic influenza A (H1N1) 2009 on the circulation of respiratory viruses 2009-2011.

    Mak, Gannon C / Wong, Ann H / Ho, Winnie Y Y / Lim, Wilina

    Influenza and other respiratory viruses

    2012  Volume 6, Issue 3, Page(s) e6–10

    Abstract: Surveillance of respiratory viruses has been conducted for many years at the public health laboratory in Hong Kong. With the occurrence of pandemic influenza A (H1N1) 2009, we observed a change in the seasonality of influenza activity with a seemingly ... ...

    Abstract Surveillance of respiratory viruses has been conducted for many years at the public health laboratory in Hong Kong. With the occurrence of pandemic influenza A (H1N1) 2009, we observed a change in the seasonality of influenza activity with a seemingly corresponding change in the activity of respiratory syncytial virus, parainfluenza virus, and adenovirus during 2009-2011. This phenomenon could most likely be explained by virus interference.
    MeSH term(s) Adolescent ; Adult ; Aged ; Child ; Child, Preschool ; Female ; Hong Kong/epidemiology ; Humans ; Influenza A Virus, H1N1 Subtype/genetics ; Influenza A Virus, H1N1 Subtype/isolation & purification ; Influenza, Human/epidemiology ; Influenza, Human/virology ; Male ; Middle Aged ; Pandemics ; Respiratory Tract Diseases/virology ; Seasons ; Sentinel Surveillance ; Viruses/classification ; Viruses/genetics ; Viruses/isolation & purification ; Young Adult
    Language English
    Publishing date 2012-01-02
    Publishing country England
    Document type Journal Article
    ZDB-ID 2274538-5
    ISSN 1750-2659 ; 1750-2640
    ISSN (online) 1750-2659
    ISSN 1750-2640
    DOI 10.1111/j.1750-2659.2011.00323.x
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