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  1. Article ; Online: Pseudomonas aeruginosa biofilm exopolysaccharides: assembly, function, and degradation.

    Gheorghita, Andreea A / Wozniak, Daniel J / Parsek, Matthew R / Howell, P Lynne

    FEMS microbiology reviews

    2023  Volume 47, Issue 6

    Abstract: The biofilm matrix is a fortress; sheltering bacteria in a protective and nourishing barrier that allows for growth and adaptation to various surroundings. A variety of different components are found within the matrix including water, lipids, proteins, ... ...

    Abstract The biofilm matrix is a fortress; sheltering bacteria in a protective and nourishing barrier that allows for growth and adaptation to various surroundings. A variety of different components are found within the matrix including water, lipids, proteins, extracellular DNA, RNA, membrane vesicles, phages, and exopolysaccharides. As part of its biofilm matrix, Pseudomonas aeruginosa is genetically capable of producing three chemically distinct exopolysaccharides - alginate, Pel, and Psl - each of which has a distinct role in biofilm formation and immune evasion during infection. The polymers are produced by highly conserved mechanisms of secretion, involving many proteins that span both the inner and outer bacterial membranes. Experimentally determined structures, predictive modelling of proteins whose structures are yet to be solved, and structural homology comparisons give us insight into the molecular mechanisms of these secretion systems, from polymer synthesis to modification and export. Here, we review recent advances that enhance our understanding of P. aeruginosa multiprotein exopolysaccharide biosynthetic complexes, and how the glycoside hydrolases/lyases within these systems have been commandeered for antimicrobial applications.
    MeSH term(s) Pseudomonas aeruginosa/genetics ; Biofilms ; Bacterial Proteins/metabolism ; Polysaccharides, Bacterial/metabolism
    Chemical Substances Bacterial Proteins ; Polysaccharides, Bacterial
    Language English
    Publishing date 2023-10-31
    Publishing country England
    Document type Review ; Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 283740-7
    ISSN 1574-6976 ; 0168-6445
    ISSN (online) 1574-6976
    ISSN 0168-6445
    DOI 10.1093/femsre/fuad060
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2165: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  2. Article ; Online: Extracellular DNA enhances biofilm integrity and mechanical properties of mucoid

    Ferguson, Danielle L / Gloag, Erin S / Parsek, Matthew R / Wozniak, Daniel J

    Journal of bacteriology

    2023  Volume 205, Issue 10, Page(s) e0023823

    Abstract: Pseudomonas ... ...

    Abstract Pseudomonas aeruginosa
    MeSH term(s) Humans ; Pseudomonas aeruginosa/metabolism ; Polysaccharides, Bacterial/metabolism ; Biofilms ; Anti-Infective Agents/metabolism ; Alginates/metabolism ; DNA/metabolism ; Pseudomonas Infections/microbiology
    Chemical Substances Polysaccharides, Bacterial ; Anti-Infective Agents ; Alginates ; DNA (9007-49-2)
    Language English
    Publishing date 2023-10-04
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2968-3
    ISSN 1098-5530 ; 0021-9193
    ISSN (online) 1098-5530
    ISSN 0021-9193
    DOI 10.1128/jb.00238-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.50: Show issues Location:
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  3. Article ; Online: Surface Dependent Inhibition of Mycobacterium abscessus by Diverse Pseudomonas aeruginosa Strains.

    Idosa, Ayantu W / Wozniak, Daniel J / Hall-Stoodley, Luanne

    Microbiology spectrum

    2022  , Page(s) e0247122

    Abstract: Both Pseudomonas aeruginosa and Mycobacterium abscessus are bacteria that cause pulmonary infection in people with inflammatory lung disease, including individuals with cystic fibrosis (CF). These bacterial species inhabit the same environmental ... ...

    Abstract Both Pseudomonas aeruginosa and Mycobacterium abscessus are bacteria that cause pulmonary infection in people with inflammatory lung disease, including individuals with cystic fibrosis (CF). These bacterial species inhabit the same environmental reservoirs (soil and water) and can be coisolated in the lungs of people with CF. We investigated the interaction of these bacteria and found an antagonistic interaction favoring P. aeruginosa that was observed in biofilms but not in planktonic cultures. This antagonism extended to multiple P. aeruginosa strains and against Mycobacterium smegmatis. We tested known P. aeruginosa mutants for genes that can play roles in interbacterial contact-dependent (type III and type VI secretion systems) and contact-independent (quorum sensing, type II secretion) antagonism pathways to interrogate the mechanism of action. Our results indicate that well-known mechanisms of interbacterial competition are not responsible for the antagonism of P. aeruginosa toward M. abscessus, suggesting a novel antibacterial strategy.
    Language English
    Publishing date 2022-11-17
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.02471-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: A bacterial pigment provides cross-species protection from H

    Liu, Yiwei / McQuillen, Eleanor A / Rana, Pranav S J B / Gloag, Erin S / Parsek, Matthew R / Wozniak, Daniel J

    Proceedings of the National Academy of Sciences of the United States of America

    2024  Volume 121, Issue 2, Page(s) e2312334121

    Abstract: Bacterial infections are often polymicrobial. ...

    Abstract Bacterial infections are often polymicrobial.
    MeSH term(s) Humans ; Animals ; Mice ; Staphylococcus aureus/genetics ; Hydrogen Peroxide/pharmacology ; Neutrophils ; Coinfection ; Staphylococcal Infections/microbiology ; Pseudomonas aeruginosa/genetics ; Biological Factors ; Biofilms
    Chemical Substances Hydrogen Peroxide (BBX060AN9V) ; Biological Factors
    Language English
    Publishing date 2024-01-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2312334121
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1148: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Author Correction: Viscoelastic properties of Pseudomonas aeruginosa variant biofilms.

    Gloag, Erin S / German, Guy K / Stoodley, Paul / Wozniak, Daniel J

    Scientific reports

    2022  Volume 12, Issue 1, Page(s) 8302

    Language English
    Publishing date 2022-05-18
    Publishing country England
    Document type Published Erratum
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-022-12599-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Standardized In vitro Assays to Visualize and Quantify Interactions between Human Neutrophils and Staphylococcus aureus Biofilms.

    Rana, Pranav S J B / Gloag, Erin S / Wozniak, Daniel J

    Journal of visualized experiments : JoVE

    2022  , Issue 184

    Abstract: Neutrophils are the first line of defense deployed by the immune system during microbial infection. In vivo, neutrophils are recruited to the site of infection where they use processes such as phagocytosis, production of reactive oxygen and nitrogen ... ...

    Abstract Neutrophils are the first line of defense deployed by the immune system during microbial infection. In vivo, neutrophils are recruited to the site of infection where they use processes such as phagocytosis, production of reactive oxygen and nitrogen species (ROS, RNS, respectively), NETosis (neutrophil extracellular trap), and degranulation to kill microbes and resolve the infection. Interactions between neutrophils and planktonic microbes have been extensively studied. There have been emerging interests in studying infections caused by biofilms in recent years. Biofilms exhibit properties, including tolerance to killing by neutrophils, distinct from their planktonic-grown counterparts. With the successful establishment of both in vitro and in vivo biofilm models, interactions between these microbial communities with different immune cells can now be investigated. Here, techniques that use a combination of traditional biofilm models and well-established neutrophil activity assays are tailored specifically to study neutrophil and biofilm interactions. Wide-field fluorescence microscopy is used to monitor the localization of neutrophils in biofilms. These biofilms are grown in static conditions, followed by the addition of neutrophils derived from human peripheral blood. The samples are stained with appropriate dyes prior to visualization under the microscope. Additionally, the production of ROS, which is one of the many neutrophil responses against pathogens, is quantified in the presence of a biofilm. The addition of immune cells to this established system will expand the understanding of host-pathogen interactions while ensuring the use of standardized and optimized conditions to measure these processes accurately.
    MeSH term(s) Biofilms ; Extracellular Traps ; Humans ; Neutrophils ; Plankton ; Reactive Oxygen Species ; Staphylococcal Infections ; Staphylococcus aureus/physiology
    Chemical Substances Reactive Oxygen Species
    Language English
    Publishing date 2022-06-08
    Publishing country United States
    Document type Journal Article ; Video-Audio Media ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2259946-0
    ISSN 1940-087X ; 1940-087X
    ISSN (online) 1940-087X
    ISSN 1940-087X
    DOI 10.3791/63773
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Pseudomonas aeruginosa Initiates a Rapid and Specific Transcriptional Response during Surface Attachment.

    Jones, Christopher J / Grotewold, Nikolas / Wozniak, Daniel J / Gloag, Erin S

    Journal of bacteriology

    2022  Volume 204, Issue 5, Page(s) e0008622

    Abstract: Chronic biofilm infections by Pseudomonas aeruginosa are a major contributor to the morbidity and mortality of patients. The formation of multicellular bacterial aggregates, called biofilms, is associated with increased resistance to antimicrobials and ... ...

    Abstract Chronic biofilm infections by Pseudomonas aeruginosa are a major contributor to the morbidity and mortality of patients. The formation of multicellular bacterial aggregates, called biofilms, is associated with increased resistance to antimicrobials and immune clearance and the persistence of infections. Biofilm formation is dependent on bacterial cell attachment to surfaces, and therefore, attachment plays a key role in chronic infections. We hypothesized that bacteria sense various surfaces and initiate a rapid, specific response to increase adhesion and establish biofilms. RNA sequencing (RNA-Seq) analysis identified transcriptional changes of adherent cells during initial attachment, identifying the bacterial response to an abiotic surface over a 1-h period. Subsequent screens investigating the most highly regulated genes in surface attachment identified 4 genes,
    MeSH term(s) Bacterial Adhesion/physiology ; Biofilms ; Humans ; Pseudomonas aeruginosa/metabolism
    Language English
    Publishing date 2022-04-25
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2968-3
    ISSN 1098-5530 ; 0021-9193
    ISSN (online) 1098-5530
    ISSN 0021-9193
    DOI 10.1128/jb.00086-22
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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.50: Show issues Location:
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  8. Article: Cross-Species Protection to Innate Immunity Mediated by A Bacterial Pigment.

    Liu, Yiwei / McQuillen, Eleanor A / Rana, Pranav S J B / Gloag, Erin S / Wozniak, Daniel J

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Bacterial infections are often polymicrobial. ...

    Abstract Bacterial infections are often polymicrobial.
    Language English
    Publishing date 2023-01-17
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.01.15.524085
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Role of Cardiac Macrophages on Cardiac Inflammation, Fibrosis and Tissue Repair.

    Lafuse, William P / Wozniak, Daniel J / Rajaram, Murugesan V S

    Cells

    2020  Volume 10, Issue 1

    Abstract: The immune system plays a pivotal role in the initiation, development and resolution of inflammation following insult or damage to organs. The heart is a vital organ which supplies nutrients and oxygen to all parts of the body. Heart failure (HF) has ... ...

    Abstract The immune system plays a pivotal role in the initiation, development and resolution of inflammation following insult or damage to organs. The heart is a vital organ which supplies nutrients and oxygen to all parts of the body. Heart failure (HF) has been conventionally described as a disease associated with cardiac tissue damage caused by systemic inflammation, arrhythmia and conduction defects. Cardiac inflammation and subsequent tissue damage is orchestrated by the infiltration and activation of various immune cells including neutrophils, monocytes, macrophages, eosinophils, mast cells, natural killer cells, and T and B cells into the myocardium. After tissue injury, monocytes and tissue-resident macrophages undergo marked phenotypic and functional changes, and function as key regulators of tissue repair, regeneration and fibrosis. Disturbance in resident macrophage functions such as uncontrolled production of inflammatory cytokines, growth factors and inefficient generation of an anti-inflammatory response or unsuccessful communication between macrophages and epithelial and endothelial cells and fibroblasts can lead to aberrant repair, persistent injury, and HF. Therefore, in this review, we discuss the role of cardiac macrophages on cardiac inflammation, tissue repair, regeneration and fibrosis.
    MeSH term(s) Animals ; Arrhythmias, Cardiac/immunology ; Arrhythmias, Cardiac/metabolism ; Cytokines/metabolism ; Diabetic Cardiomyopathies/immunology ; Diabetic Cardiomyopathies/metabolism ; Fibrosis/immunology ; Fibrosis/metabolism ; Heart/physiopathology ; Heart Injuries/immunology ; Heart Injuries/metabolism ; Homeostasis ; Humans ; Hypertension/immunology ; Hypertension/metabolism ; Inflammation/immunology ; Inflammation/metabolism ; Inflammation/pathology ; Intercellular Signaling Peptides and Proteins/metabolism ; Macrophages/cytology ; Macrophages/metabolism ; Mitochondria/immunology ; Myocardium/cytology ; Myocardium/immunology ; Regeneration
    Chemical Substances Cytokines ; Intercellular Signaling Peptides and Proteins
    Language English
    Publishing date 2020-12-31
    Publishing country Switzerland
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Review
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells10010051
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Mycobacterium abscessus biofilms have viscoelastic properties which may contribute to their recalcitrance in chronic pulmonary infections.

    Gloag, Erin S / Wozniak, Daniel J / Stoodley, Paul / Hall-Stoodley, Luanne

    Scientific reports

    2021  Volume 11, Issue 1, Page(s) 5020

    Abstract: Mycobacterium abscessus is emerging as a cause of recalcitrant chronic pulmonary infections, particularly in people with cystic fibrosis (CF). Biofilm formation has been implicated in the pathology of this organism, however the role of biofilm formation ... ...

    Abstract Mycobacterium abscessus is emerging as a cause of recalcitrant chronic pulmonary infections, particularly in people with cystic fibrosis (CF). Biofilm formation has been implicated in the pathology of this organism, however the role of biofilm formation in infection is unclear. Two colony-variants of M. abscessus are routinely isolated from CF samples, smooth (Ma
    MeSH term(s) Biofilms/growth & development ; Biomechanical Phenomena/physiology ; Cell Wall/chemistry ; Cell Wall/ultrastructure ; Elasticity ; Glycopeptides/chemistry ; Glycopeptides/isolation & purification ; Humans ; Lipopeptides/chemistry ; Lipopeptides/isolation & purification ; Mycobacterium abscessus/chemistry ; Mycobacterium abscessus/ultrastructure ; Pseudomonas aeruginosa/chemistry ; Pseudomonas aeruginosa/ultrastructure ; Rheology ; Shear Strength ; Viscosity
    Chemical Substances Glycopeptides ; Lipopeptides
    Language English
    Publishing date 2021-03-03
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-021-84525-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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