Article: Suppressive effect of Aurantii Fructus Immaturus and Atractylodis Macrocephalae Rhizoma on glutamic acid-induced autophagy of interstitial cells of Cajal.
Journal of integrative medicine
2020 Volume 18, Issue 4, Page(s) 334–343
Abstract: Objective: To investigate the effects of Aurantii Fructus Immaturus (Zhishi, ZS) and Atractylodis Macrocephalae Rhizoma (Baizhu, BZ)-containing serum on glutamate-induced autophagy in rat colonic interstitial cells of Cajal (ICCs) and to analyze the ... ...
| Abstract | Objective: To investigate the effects of Aurantii Fructus Immaturus (Zhishi, ZS) and Atractylodis Macrocephalae Rhizoma (Baizhu, BZ)-containing serum on glutamate-induced autophagy in rat colonic interstitial cells of Cajal (ICCs) and to analyze the underlying mechanism. Methods: Rat colonic ICCs cultured in vitro were identified by fluorescence and then stimulated with glutamic acid (5 mmol/L) for 24 h to establish a cell model of autophagy. The cells were then treated with different concentrations of ZSBZ-containing serum or rat serum. The viability of the ICCs was detected with cell counting kit-8 assays, and cell apoptosis rates were examined with flow cytometry. The ultrastructure and autophagosomes in the ICCs were observed using transmission electron microscopy. The effects of ZSBZ-containing serum on apoptosis-associated mediators were assessed by Western blotting and real-time quantitative polymerase chain reaction. In addition, microtubule-associated protein light chain 3 (LC3), p-phosphoinositide 3-kinase (p-PI3K), p-Akt and p-mammalian target of rapamycin (p-mTOR) expression was detected via Western blotting analysis. Results: Compared to those in the model group, ICC viability and apoptosis rates were significantly increased by ZSBZ-containing serum (P < 0.05). In addition, the expression levels of Beclin-1, LC3, p-PI3K, p-Akt and p-mTOR were significantly lower (P < 0.05) and Bcl-2 expression was higher in the ZSBZ-containing serum treatment groups than in the model group (P < 0.05). Conclusion: Our findings demonstrated that ZSBZ protects glutamic acid-stimulated ICCs, and this beneficial effect may be mediated by a reduction in autophagy via inhibition of the PI3K/Akt/mTOR pathway. |
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| MeSH term(s) | Animals ; Apoptosis ; Atractylodes/chemistry ; Autophagy ; Drugs, Chinese Herbal/pharmacology ; Glutamic Acid ; Interstitial Cells of Cajal/drug effects ; Interstitial Cells of Cajal/metabolism ; Microtubule-Associated Proteins/metabolism ; Oncogene Protein v-akt/metabolism ; Phosphatidylinositol 3-Kinases/metabolism ; Rats ; Rhizome/chemistry ; Signal Transduction ; TOR Serine-Threonine Kinases/metabolism |
| Chemical Substances | Drugs, Chinese Herbal ; LC3 protein, rat ; Microtubule-Associated Proteins ; fructus aurantii immaturus ; Glutamic Acid (3KX376GY7L) ; TOR Serine-Threonine Kinases (EC 2.7.1.1) ; mTOR protein, rat (EC 2.7.1.1) ; Oncogene Protein v-akt (EC 2.7.11.1) |
| Language | English |
| Publishing date | 2020-05-28 |
| Publishing country | Netherlands |
| Document type | Journal Article |
| ZDB-ID | 2705612-0 |
| ISSN | 2095-4964 |
| ISSN | 2095-4964 |
| DOI | 10.1016/j.joim.2020.04.005 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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