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  1. Article ; Online: Heart transplantation in a well-controlled human immunodeficiency virus infected patient: The first case of Taiwan.

    Chen, Chih-Hao / Zou, Mei-Fen / Wu, Pin-Han / Wu, Chiou-Ping / Chou, Chia-Huei / Ho, Mao-Wang / Chang, Shih-Sheng / Li, Ping-Chun

    Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi

    2022  Volume 55, Issue 5, Page(s) 982–984

    MeSH term(s) Humans ; Taiwan ; HIV Infections/drug therapy ; Antiretroviral Therapy, Highly Active ; Heart Transplantation ; HIV
    Language English
    Publishing date 2022-05-13
    Publishing country England
    Document type Case Reports ; Letter
    ZDB-ID 1497590-7
    ISSN 1995-9133 ; 1684-1182 ; 0253-2662
    ISSN (online) 1995-9133
    ISSN 1684-1182 ; 0253-2662
    DOI 10.1016/j.jmii.2022.04.014
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  2. Article: PaCDPK1, a gene encoding calcium-dependent protein kinase from orchid, Phalaenopsis amabilis, is induced by cold, wounding, and pathogen challenge.

    Tsai, Tsung-Mu / Chen, Ying-Ru / Kao, Tien-Wen / Tsay, Wen-Su / Wu, Chiou-Ping / Huang, Ding-Ding / Chen, Wen-Huei / Chang, Ching-Chun / Huang, Hao-Jen

    Plant cell reports

    2007  Volume 26, Issue 10, Page(s) 1899–1908

    Abstract: Signaling pathways, specifically calcium and calcium-dependent protein kinase (CDPK), have been implicated in the regulation of stress and developmental signals in plants. Here, we reported the isolation and characterization of an orchid, Phalaenopsis ... ...

    Abstract Signaling pathways, specifically calcium and calcium-dependent protein kinase (CDPK), have been implicated in the regulation of stress and developmental signals in plants. Here, we reported the isolation and characterization of an orchid, Phalaenopsis amabilis, CDPK gene, PaCDPK1, by using the rapid amplification of cDNA ends (RACE)-PCR technique. The full length cDNA of 2,310 bp contained an open reading frame for PaCDPK1 consisting of 593 amino acid residues. Sequence alignment indicated that PaCDPK1 shared similarities with other plant CDPKs. PaCDPK1 transcripts were expressed strongly in labellum but not in leaves and roots. In addition, the PaCDPK1 gene was transcriptionally activated in response to low temperature, wounding, and pathogen infection. To identify the regulatory role of the PaCDPK1 promoter, a construct containing the PaCDPK1 promoter fused to a beta-glucuronidase (GUS) gene was transferred into Arabidopsis by Agrobacterium-mediated transformation. GUS staining revealed that PaCDPK1/GUS expression was induced by cold, wounding, and pathogen challenge in leaves and stems of transgenic Arabidopsis. These results suggested that this PaCDPK1 gene promoter could be used as an endogenous promoter for biotechnological purposes in orchids.
    MeSH term(s) Amino Acid Sequence ; Cloning, Molecular ; Cold Temperature ; Gene Expression Regulation, Plant ; Molecular Sequence Data ; Orchidaceae/enzymology ; Orchidaceae/genetics ; Orchidaceae/microbiology ; Orchidaceae/physiology ; Plant Diseases/genetics ; Plant Proteins/genetics ; Plant Proteins/metabolism ; Promoter Regions, Genetic ; Protein Kinases/genetics ; Protein Kinases/metabolism ; Sequence Alignment
    Chemical Substances Plant Proteins ; Protein Kinases (EC 2.7.-) ; calcium-dependent protein kinase (EC 2.7.1.-)
    Language English
    Publishing date 2007-06-26
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 8397-5
    ISSN 1432-203X ; 0721-7714 ; 0721-085X
    ISSN (online) 1432-203X
    ISSN 0721-7714 ; 0721-085X
    DOI 10.1007/s00299-007-0389-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: PaCDPK1, a gene encoding calcium-dependent protein kinase from orchid, Phalaenopsis amabilis, is induced by cold, wounding, and pathogen challenge

    Tsai, Tsung-Mu / Chen, Ying-Ru / Kao, Tien-Wen / Tsay, Wen-Su / Wu, Chiou-Ping / Huang, Ding-Ding / Chen, Wen-Huei / Chang, Ching-Chun / Huang, Hao-Jen

    Plant cell reports. 2007 Oct., v. 26, no. 10

    2007  

    Abstract: Signaling pathways, specifically calcium and calcium-dependent protein kinase (CDPK), have been implicated in the regulation of stress and developmental signals in plants. Here, we reported the isolation and characterization of an orchid, Phalaenopsis ... ...

    Abstract Signaling pathways, specifically calcium and calcium-dependent protein kinase (CDPK), have been implicated in the regulation of stress and developmental signals in plants. Here, we reported the isolation and characterization of an orchid, Phalaenopsis amabilis, CDPK gene, PaCDPK1, by using the rapid amplification of cDNA ends (RACE)-PCR technique. The full length cDNA of 2,310 bp contained an open reading frame for PaCDPK1 consisting of 593 amino acid residues. Sequence alignment indicated that PaCDPK1 shared similarities with other plant CDPKs. PaCDPK1 transcripts were expressed strongly in labellum but not in leaves and roots. In addition, the PaCDPK1 gene was transcriptionally activated in response to low temperature, wounding, and pathogen infection. To identify the regulatory role of the PaCDPK1 promoter, a construct containing the PaCDPK1 promoter fused to a β-glucuronidase (GUS) gene was transferred into Arabidopsis by Agrobacterium-mediated transformation. GUS staining revealed that PaCDPK1/GUS expression was induced by cold, wounding, and pathogen challenge in leaves and stems of transgenic Arabidopsis. These results suggested that this PaCDPK1 gene promoter could be used as an endogenous promoter for biotechnological purposes in orchids.
    Keywords Orchidaceae ; Erwinia
    Language English
    Dates of publication 2007-10
    Size p. 1899-1908.
    Publisher Springer-Verlag
    Publishing place Berlin/Heidelberg
    Document type Article
    ZDB-ID 8397-5
    ISSN 1432-203X ; 0721-085X ; 0721-7714
    ISSN (online) 1432-203X
    ISSN 0721-085X ; 0721-7714
    DOI 10.1007/s00299-007-0389-5
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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