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  1. Article: Characterization of vB_ValM_PVA8, a broad-host-range bacteriophage infecting

    Fu, Jingyun / Li, Ying / Zhao, Lihong / Wu, Chunguang / He, Zengguo

    Frontiers in microbiology

    2023  Volume 14, Page(s) 1105924

    Abstract: Phage therapy was taken as an alternative strategy to antibiotics in shrimp farming for the control ... ...

    Abstract Phage therapy was taken as an alternative strategy to antibiotics in shrimp farming for the control of
    Language English
    Publishing date 2023-05-12
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2023.1105924
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Characterization and Genomic Analysis of a Bacteriophage with Potential in Lysing Vibrio alginolyticus

    Fu, Jingyun / Li, Ying / Zhao, Lihong / Wu, Chunguang / He, Zengguo

    Viruses

    2022  Volume 15, Issue 1

    Abstract: Vibrio alginolyticus is one of the major pathogens causing vibriosis to a variety of aquatic animals as well as bringing about severe food safety concerns. Nowadays, phage therapy has received increasing attention as an alternative to the antibiotics ... ...

    Abstract Vibrio alginolyticus is one of the major pathogens causing vibriosis to a variety of aquatic animals as well as bringing about severe food safety concerns. Nowadays, phage therapy has received increasing attention as an alternative to the antibiotics that have being limited for use in aquaculture industries. In this work, a potent bacteriophage, vB_ValM_PVA23 (PVA23), which efficiently infects pathogenic strains of V. alginolyticus, was isolated from sewage water and characterized by microbiological and genomic analyses. Based on the transmission electronic observation, the phage was characterized to be the Myoviridae family. It has a latent period of 10 min and a burst size of 203 PFUs/infected bacterium, and was stable over a broad pH range (5.0−11.0) and a wide temperature span (−80 °C to 60 °C), respectively. Genome sequencing results show that PVA23 has a 246,962-bp double-stranded DNA with a G + C content of 41.25%. The lab and plant shrimp farming trials demonstrated that phage preparation derived from PVA23 out-performed the chemical disinfectant iodine treatment in the prevention of V. alginolyticus propagation, and the phage application could rapidly yet significantly reduce the level of V. alginolyticus in the pond within 12 h, with negligible rebound observed. These results suggests that phage PVA23 has the potential to be used as an anti-V. alginolyticus agent in aquaculture industries.
    MeSH term(s) Animals ; Bacteriophages/genetics ; Vibrio alginolyticus/genetics ; Genomics ; Myoviridae/genetics ; Vibrio Infections/microbiology ; Genome, Viral
    Language English
    Publishing date 2022-12-31
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v15010135
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Identification of TMEM53 as a novel SADS-CoV restriction factor that targets viral RNA-dependent RNA polymerase.

    Yao, Yu-Lin / Luo, Yun / Wang, Qi / Geng, Rong / Chen, Ying / Liu, Mei-Qin / Li, Bei / Chen, Jing / Wu, Chun-Guang / Jia, Jing-Kun / Luo, Jing-Yi / He, Yan-Tong / Jiang, Ting-Ting / Zhu, Yan / Hu, Ben / Zhou, Peng / Shi, Zheng-Li

    Emerging microbes & infections

    2023  Volume 12, Issue 2, Page(s) 2249120

    Abstract: ... ...

    Abstract ABSTRACT
    MeSH term(s) Animals ; Humans ; Alphacoronavirus/genetics ; Antiviral Agents/pharmacology ; Coronavirus Infections ; RNA-Dependent RNA Polymerase/genetics ; Swine ; Membrane Proteins/genetics
    Chemical Substances Antiviral Agents ; RNA-Dependent RNA Polymerase (EC 2.7.7.48) ; Membrane Proteins
    Language English
    Publishing date 2023-08-16
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2681359-2
    ISSN 2222-1751 ; 2222-1751
    ISSN (online) 2222-1751
    ISSN 2222-1751
    DOI 10.1080/22221751.2023.2249120
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: A bat MERS-like coronavirus circulates in pangolins and utilizes human DPP4 and host proteases for cell entry.

    Chen, Jing / Yang, Xinglou / Si, Haorui / Gong, Qianchun / Que, Tengcheng / Li, Jing / Li, Yang / Wu, Chunguang / Zhang, Wei / Chen, Ying / Luo, Yun / Zhu, Yan / Li, Bei / Luo, Dongsheng / Hu, Ben / Lin, Haofeng / Jiang, Rendi / Jiang, Tingting / Li, Qian /
    Liu, Meiqin / Xie, Shizhe / Su, Jia / Zheng, Xiaoshuang / Li, Ang / Yao, Yulin / Yang, Yong / Chen, Panyu / Wu, Aiqiong / He, Meihong / Lin, Xinhua / Tong, Yigang / Hu, Yanling / Shi, Zheng-Li / Zhou, Peng

    Cell

    2023  Volume 186, Issue 4, Page(s) 850–863.e16

    Abstract: It is unknown whether pangolins, the most trafficked mammals, play a role in the zoonotic transmission of bat coronaviruses. We report the circulation of a novel MERS-like coronavirus in Malayan pangolins, named Manis javanica HKU4-related coronavirus ( ... ...

    Abstract It is unknown whether pangolins, the most trafficked mammals, play a role in the zoonotic transmission of bat coronaviruses. We report the circulation of a novel MERS-like coronavirus in Malayan pangolins, named Manis javanica HKU4-related coronavirus (MjHKU4r-CoV). Among 86 animals, four tested positive by pan-CoV PCR, and seven tested seropositive (11 and 12.8%). Four nearly identical (99.9%) genome sequences were obtained, and one virus was isolated (MjHKU4r-CoV-1). This virus utilizes human dipeptidyl peptidase-4 (hDPP4) as a receptor and host proteases for cell infection, which is enhanced by a furin cleavage site that is absent in all known bat HKU4r-CoVs. The MjHKU4r-CoV-1 spike shows higher binding affinity for hDPP4, and MjHKU4r-CoV-1 has a wider host range than bat HKU4-CoV. MjHKU4r-CoV-1 is infectious and pathogenic in human airways and intestinal organs and in hDPP4-transgenic mice. Our study highlights the importance of pangolins as reservoir hosts of coronaviruses poised for human disease emergence.
    MeSH term(s) Animals ; Humans ; Mice ; Chiroptera ; Coronavirus Infections/veterinary ; Coronavirus Infections/virology ; Dipeptidyl Peptidase 4/genetics ; Dipeptidyl Peptidase 4/metabolism ; Endopeptidases/metabolism ; Middle East Respiratory Syndrome Coronavirus/genetics ; Middle East Respiratory Syndrome Coronavirus/metabolism ; Pangolins ; Peptide Hydrolases/metabolism ; Receptors, Virus/metabolism ; Virus Internalization ; Coronavirus/physiology
    Chemical Substances Dipeptidyl Peptidase 4 (EC 3.4.14.5) ; DPP4 protein, human (EC 3.4.14.5) ; Endopeptidases (EC 3.4.-) ; Peptide Hydrolases (EC 3.4.-) ; Receptors, Virus
    Language English
    Publishing date 2023-02-26
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 187009-9
    ISSN 1097-4172 ; 0092-8674
    ISSN (online) 1097-4172
    ISSN 0092-8674
    DOI 10.1016/j.cell.2023.01.019
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1167: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (1.OG)
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    Zs.MG 58: Show issues

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  5. Article: Tricalcaria Han gen. nov., a remarkable new genus with three hind-tibial spurs belonging to the tribe Trichopterygini, with description of a new species from China (Lepidoptera: Geometridae: Larentiinae)

    XUE, Dayong / WU, Chunguang / HAN, Hongxiang

    Entomological science. 2008 Dec., v. 11, no. 4

    2008  

    Abstract: Tricalcaria Han gen. nov. and its type species Tricalcaria stueningi Han sp. nov. are described from China. Morphological characters, including those of the male and female genitalia, are figured. The main diagnostic characters of Tricalcaria are: hind ... ...

    Abstract Tricalcaria Han gen. nov. and its type species Tricalcaria stueningi Han sp. nov. are described from China. Morphological characters, including those of the male and female genitalia, are figured. The main diagnostic characters of Tricalcaria are: hind tibia with three spurs in both male and female; ventral margin of costal protrusion bearing a series of long, hook-like, sclerotized setae in the male genitalia; female papilla analis modified and bearing over part of its surface broad blade-like sclerotized setae. The wing pattern, venation, and genitalia of this genus are compared with those of the most closely related genera, Tristeirometa Holloway, 1997, Hypocometa Warren, 1896, and Phthonoloba Warren, 1893. Tribal placement is discussed, with the conclusion that the new genus should be placed in the tribe Trichopterygini.
    Keywords Lepidoptera ; new genus ; new species
    Language English
    Dates of publication 2008-12
    Size p. 409-414.
    Publisher Blackwell Publishing Asia
    Publishing place Melbourne, Australia
    Document type Article
    ZDB-ID 2105599-3
    ISSN 1479-8298 ; 1343-8786
    ISSN (online) 1479-8298
    ISSN 1343-8786
    DOI 10.1111/j.1479-8298.2008.00287.x
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: 50 Fälle von Gastroptose, die mit Moxibustionstherapie behandelt wurde

    Wu, Chunguang / Wang, Zhili / Ye, Xiuhua / Ye, Yunhua

    Zeitschrift für traditionelle chinesische medizin

    2000  Volume 9, Issue 1, Page(s) 29

    Language German
    Document type Article
    ZDB-ID 1330658-3
    ISSN 0941-3448
    Database Current Contents Medicine

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4582: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (2.OG)
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