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  1. Article: The essential role of hydrophobic interaction within extracellular polymeric substances in auto-aggregation of P. stutzeri strain XL-2

    Wu, Dan qing / Ding, Xue Song / Zhao, Bin / An, Qiang / Guo, Jin Song

    International biodeterioration & biodegradation. 2022 July, v. 171

    2022  

    Abstract: To enrich the understanding of bio-aggregates formation, the mechanism of auto-aggregation is one of the concerns in wastewater treatment. The auto-aggregation ability by P. stutzeri strain XL-2 was mainly attributed to the self-secretion of ... ...

    Abstract To enrich the understanding of bio-aggregates formation, the mechanism of auto-aggregation is one of the concerns in wastewater treatment. The auto-aggregation ability by P. stutzeri strain XL-2 was mainly attributed to the self-secretion of extracellular polymeric substances (EPS). A comprehensive correlation analysis indicates that hydrophobic interaction within EPS played essential roles in auto-aggregation. To give insights into the underlying mechanisms, tightly bound EPS (TB-EPS), loosely bound EPS (LB-EPS) and soluble EPS (S-EPS) were characterized by colorimetry, hydrophobicity, enzymatic hydrolysis, FT-IR spectroscopy and XPS analysis, showing that proteins in TB-EPS comprised a high proportion of hydrophobic carbon-containing (C-containing) functional groups. Further amino acids analysis revealed that these C-containing functional groups might derive from the hydrophobic R groups of amino acids, which significantly affected the surface hydrophobicity of proteins in TB-EPS and thus promoted the auto-aggregation of strain XL-2. This work provides an in-deep understanding of the auto-aggregation mechanism by strain XL-2, which may enrich the understanding of microbial aggregates formation and lead to further application of strain XL-2 in wastewater treatment processes.
    Keywords Fourier transform infrared spectroscopy ; biodegradation ; colorimetry ; enzymatic hydrolysis ; hydrophobic bonding ; hydrophobicity ; polymers ; wastewater treatment
    Language English
    Dates of publication 2022-07
    Publishing place Elsevier Ltd
    Document type Article
    ISSN 0964-8305
    DOI 10.1016/j.ibiod.2022.105404
    Database NAL-Catalogue (AGRICOLA)

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  2. Article: Evaluation of diffusion in gel entrapment cell culture within hollow fibers.

    Wu, Dan-Qing / Zhang, Guo-Liang / Shen, Chong / Zhao, Qian / Li, Hui / Meng, Qin

    World journal of gastroenterology

    2005  Volume 11, Issue 11, Page(s) 1599–1604

    Abstract: Aim: To investigate diffusion in mammalian cell culture by gel entrapment within hollow fibers.: Methods: Freshly isolated rat hepatocytes or human oral epidermoid carcinoma (KB) cells were entrapped in type I collagen solutions and statically ... ...

    Abstract Aim: To investigate diffusion in mammalian cell culture by gel entrapment within hollow fibers.
    Methods: Freshly isolated rat hepatocytes or human oral epidermoid carcinoma (KB) cells were entrapped in type I collagen solutions and statically cultured inside microporous and ultrafiltration hollow fibers. During the culture time collagen gel contraction, cell viability and specific function were assessed. Effective diffusion coefficients of glucose in cell-matrix gels were determined by lag time analysis in a diffusion cell.
    Results: Significant gel contractions occurred in the collagen gels by entrapment of either viable hepatocytes or KB cells. And the gel contraction caused a significant reduction on effective diffusion coefficient of glucose. The cell viability assay of both hepatocytes and KB cells statically cultured in hollow fibers by collagen entrapment further confirmed the existence of the inhibited mass transfer by diffusion. Urea was secreted about 50% more by hepatocytes entrapped in hollow fibers with pore size of 0.1 mum than that in hollow fibers with MWCO of 100 ku.
    Conclusion: Cell-matrix gel and membrane pore size are the two factors relevant to the limited mass transfer by diffusion in such gel entrapment of mammalian cell culture.
    MeSH term(s) Animals ; Carcinoma, Squamous Cell ; Cells, Immobilized ; Collagen ; Diffusion ; Diffusion Chambers, Culture/instrumentation ; Diffusion Chambers, Culture/methods ; Evaluation Studies as Topic ; Gels ; Hepatocytes/cytology ; Hepatocytes/metabolism ; Humans ; Mouth Neoplasms ; Polymers ; Rats ; Sulfones ; Tumor Cells, Cultured
    Chemical Substances Gels ; Polymers ; Sulfones ; polysulfone P 1700 (25135-51-7) ; Collagen (9007-34-5)
    Language English
    Publishing date 2005-03-21
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2185929-2
    ISSN 2219-2840 ; 1007-9327
    ISSN (online) 2219-2840
    ISSN 1007-9327
    DOI 10.3748/wjg.v11.i11.1599
    Database MEDical Literature Analysis and Retrieval System OnLINE

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