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Article ; Online: Qi Wei anti-burn Tincture remodels liver metabolic pathways and treats burn wounds efficiently.

Wang, Shuai / Zhou, Hui / Cui, Weiqi / Zhang, Junwei / Wu, Deqiao / Zhang, Nan / Xu, Xia

Journal of burn care & research : official publication of the American Burn Association

2022

Abstract: This work aims to elucidate the molecular mechanism of Qi Wei anti-burn Tincture (QW) on wound healing in burnt mice using metabolomics and molecular biology techniques. A scald model was first established in Kunming mice. After treatment, biochemical ... ...

Abstract	This work aims to elucidate the molecular mechanism of Qi Wei anti-burn Tincture (QW) on wound healing in burnt mice using metabolomics and molecular biology techniques. A scald model was first established in Kunming mice. After treatment, biochemical indicators for liver function and burnt skin tissues were then evaluated via biochemical detection and HE staining respectively. Liver tissues were further analyzed for differential metabolites, inflammatory factors, and mRNA levels of cytokines using metabolomics and molecular biology techniques. Involved metabolic pathways were also identified using software. Qi Wei anti-burn Tincture treatment did promote the healing of the burn wounds on Kunming mice with a downregulation of ALP, ALT, AST to normal levels. In mouse liver tissue, the contents of glutamine, aspartic acid, succinic acid and citrulline were significantly reduced, while the contents of 5-hydroxyproline, taurine, hypotaurine and glutamic acid significantly increased. These major differential compounds are involved in the arginine metabolic pathway, nitrogen excretion, and the metabolism of taurine and hypotaurine, suggesting that Qi Wei anti-burn Tincture reprogramed the above metabolic processes in the liver. Furthermore, the application of Qi Wei anti-burn Tincture increased the expression of TGF-β1 and FGF-2, and reduced the levels of TNF-α, IL-1β, IL-6 and reactive oxygen species in the liver of mice induced by burn injury. This study found that Qi Wei anti-burn Tincture treatment promoted metabolic pathway remodeling in liver, which might be a potential mechanism for Qi Wei anti-burn Tincture to treat burn wounds.
Language	English
Publishing date	2022-11-19
Publishing country	England
Document type	Journal Article
ZDB-ID	2224246-6
ISSN	1559-0488 ; 1559-047X
ISSN (online)	1559-0488
ISSN	1559-047X
DOI	10.1093/jbcr/irac175
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Article ; Online: Artemisia argyi H.Lév. & Vaniot essential oil induces ferroptosis in pancreatic cancer cells via up-regulation of TFR1 and depletion of γ-glutamyl cycle.

Zhang, Nan / Zhang, Junwei / Cui, Weiqi / Wu, Deqiao / Zhang, Jingxian / Wei, Bo / Qu, Lingbo / Xu, Xia

Fitoterapia

2023 Volume 168, Page(s) 105522

Abstract: Artemisia argyi H.Lév. & Vaniot, a traditional Chinese medicine with a history spanning over two millennia, has been extensively used in folk medicine to treat dysmenorrhea, uterine bleeding and inflammation. Recent studies have demonstrated that the ... ...

Abstract	Artemisia argyi H.Lév. & Vaniot, a traditional Chinese medicine with a history spanning over two millennia, has been extensively used in folk medicine to treat dysmenorrhea, uterine bleeding and inflammation. Recent studies have demonstrated that the essential oil extracted from Artemisia argyi H.Lév. & Vaniot, known as AAEO, exhibits significant anti-tumor properties against liver and lung cancers. There is a scarcity of research on the potential impact of AAEO on pancreatic cancer (PC) cells. In this study, UPLC-MS/MS-based metabolomics method was established to evaluate the effect of AAEO on the proliferation of PC cells. The differential compounds included 5-oxoproline, glutamate, γ-glutamylcysteine, glutathione, arachidonic acid, adrenal acid and linoleic acid were detected by metabolomics, enriching in the γ-glutamyl cycle and polyunsaturated fatty acid metabolism, which were closely related to ferroptosis. Meanwhile, AAEO dramatically increased the levels of intracellular iron ion via up-regulation of TFR1, augmented reactive oxygen species and malondialdehyde in a dose-dependent manner by down-regulation of γ-glutamyl cycle through decreasing expressions of SLC7A11. Additionally, β-caryophyllene oxide, one of the main components of AAEO, could covalently bind to Cys in SW1990 cells to form a conjugate Cpo-Cys, resulting in the inhibition of glutathione synthesis. Importantly, the ferroptosis inhibitor deferoxamine significantly blocked the inhibitory effect of AAEO on SW1990 cells. Meanwhile, β-caryophyllene oxide, dihydro-β-ionone and α-bisabolol had strong binding force with GPX4, SLC7A11 and TFR1, respectively. These findings showed that AAEO induced ferroptosis via regulation of γ-glutamyl cycle by SLC7A11 and iron disorders by TFR1. Our study discovered AAEO as a potential therapeutic approach to induce ferroptosis to prevent or treat PC.
MeSH term(s)	Humans ; Oils, Volatile/pharmacology ; Oils, Volatile/chemistry ; Artemisia/chemistry ; Ferroptosis ; Up-Regulation ; Chromatography, Liquid ; Tandem Mass Spectrometry ; Molecular Structure ; Pancreatic Neoplasms/drug therapy
Chemical Substances	Oils, Volatile ; caryophyllene oxide (S2XU9K448U)
Language	English
Publishing date	2023-05-09
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	412385-2
ISSN	1873-6971 ; 0367-326X
ISSN (online)	1873-6971
ISSN	0367-326X
DOI	10.1016/j.fitote.2023.105522
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Article ; Online: Hepatoprotective effect of Artemisia Argyi essential oil on bisphenol A‐induced hepatotoxicity via inhibition of ferroptosis in mice

Cui, Weiqi / Zhou, Hui / Zhang, Jingxian / Zhang, Junwei / Wu, Deqiao / Rong, Ying / Liu, Fanglin / Liu, Junhui / Liu, Haiyan / Wei, Bo / Tang, Youcai / Liao, Xinglin / Xu, Xia

Environmental Toxicology. 2023 Oct., v. 38, no. 10 p.2416-2428

2023

Abstract: The environmental pollutant bisphenol A (BPA), used in the manufacture of plastic packaging materials for various diets, is widely distributed in the environment and causes severe hepatotoxicity by inducing oxidative stress. Artemisia argyi essential oil ...

Abstract	The environmental pollutant bisphenol A (BPA), used in the manufacture of plastic packaging materials for various diets, is widely distributed in the environment and causes severe hepatotoxicity by inducing oxidative stress. Artemisia argyi essential oil (AAEO), a volatile oil component isolated from Artemisia argyi H.Lév. & Vaniot, has pharmacological effects, especially for hepatoprotective actions. However, the potential effect of AAEO in BPA induced hepatotoxicity has not been characterized. First, we analyzed the chemical composition in AAEO by gas chromatography–mass spectrometry. Herein, we investigated the effect of AAEO on hepatic metabolic changes in mice exposed to BPA. Results showed that compared with the BPA group, AAEO could reduce the level of liver function enzymes in BPA mice serum, and ameliorate hepatic lesions and fibrosis. Additionally, 20 differential metabolites screened by metabolomics were mainly involved in the reprogramming of glutathione metabolism, purine metabolism, and polyunsaturated fatty acid synthesis. Moreover, AAEO could reduce hepatic ferroptosis induced by BPA, as demonstrated by reducing xanthine oxidase activity, up‐regulating the activities of glutathione peroxidase 4 (GPX4), superoxide dismutase, and catalase and the expression of SLC7A11 to promote the glutathione synthetic, while inhibiting transferrin receptor 1 (TFR1) expression to reduce the accumulation of Fe²⁺ in cells. Therefore, our study identified AAEO as a hepatic protectant against BPA‐induced hepatotoxicity by reversing the occurrence of ferroptosis.
Keywords	Artemisia argyi ; bisphenol A ; blood serum ; catalase ; chemical composition ; ecotoxicology ; essential oils ; ferroptosis ; fibrosis ; gas chromatography-mass spectrometry ; glutathione ; hepatoprotective effect ; hepatotoxicity ; liver function ; manufacturing ; metabolism ; metabolites ; metabolomics ; oxidative stress ; phospholipid-hydroperoxide glutathione peroxidase ; pollutants ; polyunsaturated fatty acids ; superoxide dismutase ; transferrin receptors ; xanthine oxidase
Language	English
Dates of publication	2023-10
Size	p. 2416-2428.
Publishing place	John Wiley & Sons, Inc.
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	1463449-1
ISSN	1522-7278 ; 1520-4081
ISSN (online)	1522-7278
ISSN	1520-4081
DOI	10.1002/tox.23877
Database	NAL-Catalogue (AGRICOLA)

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Article: Ponicidin suppresses pancreatic cancer growth by inducing ferroptosis: Insight gained by mass spectrometry-based metabolomics

Cui, Weiqi / Zhang, Junwei / Wu, Deqiao / Zhang, Jingxian / Zhou, Hui / Rong, Ying / Liu, Fanglin / Wei, Bo / Xu, Xia

Phytomedicine. 2022 Apr., v. 98

2022

Abstract: Pancreatic cancer is one of the most common malignant tumors of the digestive tract. Ponicidin, a tetracyclic diterpenoid active ingredient extracted from the traditional phytomedicine Rubescens, has high safety and great inhibitory effect on the ... ...

Abstract	Pancreatic cancer is one of the most common malignant tumors of the digestive tract. Ponicidin, a tetracyclic diterpenoid active ingredient extracted from the traditional phytomedicine Rubescens, has high safety and great inhibitory effect on the proliferation of a variety of cancer cells, especially malignant tumor cells of the digestive tract. However, the inhibitory effect and mechanism of ponicidin on pancreatic cancer cells is still unclear. Our study aimed to use metabonomics technology to analyze and explore the suppressive effect of ponidicin against pancreatic cancer cells. MTT and flow cytometry were conducted to study the potential effect of ponicidin on SW1990 cells. Secondly, UPLC-MS/MS was used to analyze the small molecule metabolites and relevant differential metabolic pathways induced by ponicidin treatment. Furthermore, through the determination of glutathione peroxidase 4 (GPX4) activity and molecular docking simulation experiments, the effects of intracellular GPX4 activity and GSH/GSSG ratio after ponicidin were evaluated. Finally, the determination of the content of iron ions and malondialdehyde in cells, and the experiment of the effect of ferroptosis inhibitors on cell viability, the effect of ponicidin on the induction of ferroptosis in SW1990 cells was also detected. The IC₅₀ of ponicidin on SW1990 cells was 20 μM, which could significantly induce cell apoptosis and arrest the cells in G₂/M phase. Metabolomics results showed that the contents of endogenous small molecules such as gamma-glutamylcysteine, 5-oxoproline, glutamic acid, reduced glutathione (GSH), oxidized glutathione (GSSG) and arachidonic acid have changed significantly. Main differential compounds were involved in the gamma-glutamyl cycle and polyunsaturated fatty acid metabolism of pancreatic cancer cell lines. Additionally, ponicidin could covalently bind to GSH in SW1990 cells to form a conjugate Pon-GSH, which further reduced the content of free GSH and GPX4 activity in cells. Notably, ponicidin dose-dependently increased levels of iron ions, malondialdehyde and reactive oxygen species in SW1990 cells, and the ferroptosis inhibitors could significantly block the effects of ponicidin on the proliferation of SW1990 cells. Ponicidin could suppress the pancreatic cancer cell proliferation via inducing ferroptosis by inhibiting the gamma-glutamyl cycle and regulating the polyunsaturated fatty acid metabolism in SW1990 cells.
Keywords	active ingredients ; apoptosis ; arachidonic acid ; cell proliferation ; cell viability ; chemical bonding ; digestive tract ; diterpenoids ; fatty acid metabolism ; ferroptosis ; flow cytometry ; glutamic acid ; glutathione ; malondialdehyde ; metabolites ; metabolomics ; neoplasm cells ; pancreatic neoplasms ; phospholipid-hydroperoxide glutathione peroxidase ; reactive oxygen species
Language	English
Dates of publication	2022-04
Publishing place	Elsevier GmbH
Document type	Article
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2022.153943
Database	NAL-Catalogue (AGRICOLA)

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Zs.A 4115: Show issues

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Article ; Online: Ponicidin suppresses pancreatic cancer growth by inducing ferroptosis: Insight gained by mass spectrometry-based metabolomics.

Cui, Weiqi / Zhang, Junwei / Wu, Deqiao / Zhang, Jingxian / Zhou, Hui / Rong, Ying / Liu, Fanglin / Wei, Bo / Xu, Xia

Phytomedicine : international journal of phytotherapy and phytopharmacology

2022 Volume 98, Page(s) 153943

Abstract: Background: Pancreatic cancer is one of the most common malignant tumors of the digestive tract. Ponicidin, a tetracyclic diterpenoid active ingredient extracted from the traditional phytomedicine Rubescens, has high safety and great inhibitory effect ... ...

Abstract	Background: Pancreatic cancer is one of the most common malignant tumors of the digestive tract. Ponicidin, a tetracyclic diterpenoid active ingredient extracted from the traditional phytomedicine Rubescens, has high safety and great inhibitory effect on the proliferation of a variety of cancer cells, especially malignant tumor cells of the digestive tract. However, the inhibitory effect and mechanism of ponicidin on pancreatic cancer cells is still unclear. Our study aimed to use metabonomics technology to analyze and explore the suppressive effect of ponidicin against pancreatic cancer cells. Methods: MTT and flow cytometry were conducted to study the potential effect of ponicidin on SW1990 cells. Secondly, UPLC-MS/MS was used to analyze the small molecule metabolites and relevant differential metabolic pathways induced by ponicidin treatment. Furthermore, through the determination of glutathione peroxidase 4 (GPX4) activity and molecular docking simulation experiments, the effects of intracellular GPX4 activity and GSH/GSSG ratio after ponicidin were evaluated. Finally, the determination of the content of iron ions and malondialdehyde in cells, and the experiment of the effect of ferroptosis inhibitors on cell viability, the effect of ponicidin on the induction of ferroptosis in SW1990 cells was also detected. Results: The IC Conclusion: Ponicidin could suppress the pancreatic cancer cell proliferation via inducing ferroptosis by inhibiting the gamma-glutamyl cycle and regulating the polyunsaturated fatty acid metabolism in SW1990 cells.
Language	English
Publishing date	2022-01-14
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1205240-1
ISSN	1618-095X ; 0944-7113
ISSN (online)	1618-095X
ISSN	0944-7113
DOI	10.1016/j.phymed.2022.153943
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Article ; Online: Hepatoprotective effect of Artemisia Argyi essential oil on bisphenol A-induced hepatotoxicity via inhibition of ferroptosis in mice.

Cui, Weiqi / Zhou, Hui / Zhang, Jingxian / Zhang, Junwei / Wu, Deqiao / Rong, Ying / Liu, Fanglin / Liu, Junhui / Liu, Haiyan / Wei, Bo / Tang, Youcai / Liao, Xinglin / Xu, Xia

Environmental toxicology

2023 Volume 38, Issue 10, Page(s) 2416–2428

Abstract	The environmental pollutant bisphenol A (BPA), used in the manufacture of plastic packaging materials for various diets, is widely distributed in the environment and causes severe hepatotoxicity by inducing oxidative stress. Artemisia argyi essential oil (AAEO), a volatile oil component isolated from Artemisia argyi H.Lév. & Vaniot, has pharmacological effects, especially for hepatoprotective actions. However, the potential effect of AAEO in BPA induced hepatotoxicity has not been characterized. First, we analyzed the chemical composition in AAEO by gas chromatography-mass spectrometry. Herein, we investigated the effect of AAEO on hepatic metabolic changes in mice exposed to BPA. Results showed that compared with the BPA group, AAEO could reduce the level of liver function enzymes in BPA mice serum, and ameliorate hepatic lesions and fibrosis. Additionally, 20 differential metabolites screened by metabolomics were mainly involved in the reprogramming of glutathione metabolism, purine metabolism, and polyunsaturated fatty acid synthesis. Moreover, AAEO could reduce hepatic ferroptosis induced by BPA, as demonstrated by reducing xanthine oxidase activity, up-regulating the activities of glutathione peroxidase 4 (GPX4), superoxide dismutase, and catalase and the expression of SLC7A11 to promote the glutathione synthetic, while inhibiting transferrin receptor 1 (TFR1) expression to reduce the accumulation of Fe
MeSH term(s)	Mice ; Animals ; Artemisia/chemistry ; Oils, Volatile/pharmacology ; Ferroptosis ; Glutathione ; Chemical and Drug Induced Liver Injury/prevention & control
Chemical Substances	Oils, Volatile ; bisphenol A (MLT3645I99) ; Glutathione (GAN16C9B8O)
Language	English
Publishing date	2023-06-22
Publishing country	United States
Document type	Journal Article
ZDB-ID	1463449-1
ISSN	1522-7278 ; 1520-4081
ISSN (online)	1522-7278
ISSN	1520-4081
DOI	10.1002/tox.23877
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Zs.A 2676: Show issues

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Article ; Online: Nano-drug delivery system with enhanced tumour penetration and layered anti-tumour efficacy.

Zhang, Nan / Feng, Nannan / Xin, Xiangying / Zhang, Junwei / Wu, Deqiao / Jiang, Qianqian / Yu, Tong / Gao, Ming / Zhao, Siyuan / Yang, Hui / Tian, Qingfeng

Nanomedicine : nanotechnology, biology, and medicine

2022 Volume 45, Page(s) 102592

Abstract: The low delivery efficiency of nano-drugs and limited tumour penetration are still huge challenges in treating solid tumours. Herein, we developed a pH-responsive nano-drug delivery system, CALS/PDMA@DOX, with a size conversion-layered delivery function. ...

Abstract	The low delivery efficiency of nano-drugs and limited tumour penetration are still huge challenges in treating solid tumours. Herein, we developed a pH-responsive nano-drug delivery system, CALS/PDMA@DOX, with a size conversion-layered delivery function. The system is composed of a pH-responsive cationic liposome loaded with DOX (CALS) and a polyamidoamine dendrimer loaded with DOX (PAMAM@DOX) modified with 2,3-dimethylmaleic anhydride (PDMA@DOX) using electrostatic adsorption. In the tumour microenvironment, the positively-charged large-size CALS and the positively-charged small-size PAMAM@DOX were dissociated to exert anti-tumour effects. CALS preferentially targeted tumour angiogenesis endothelial cells. Because of its small size and positive charge, PAMAM@DOX showed excellent tumour penetration. Significant tumour suppression by the system in vivo was confirmed in a 4T1 tumour xenograft mouse model. This pH-triggered size-switching layered delivery nanosystem is a safe and effective cancer treatment delivery platform that improves drug permeability and therapeutic efficacy.
MeSH term(s)	Animals ; Cell Line, Tumor ; Dendrimers ; Doxorubicin/pharmacology ; Doxorubicin/therapeutic use ; Drug Carriers ; Drug Delivery Systems ; Endothelial Cells/pathology ; Humans ; Hydrogen-Ion Concentration ; Liposomes ; Mice ; Nanoparticle Drug Delivery System ; Nanoparticles/therapeutic use ; Neoplasms/drug therapy ; Neoplasms/pathology ; Tumor Microenvironment
Chemical Substances	Dendrimers ; Drug Carriers ; Liposomes ; Nanoparticle Drug Delivery System ; Doxorubicin (80168379AG)
Language	English
Publishing date	2022-07-27
Publishing country	United States
Document type	Journal Article
ZDB-ID	2183417-9
ISSN	1549-9642 ; 1549-9634
ISSN (online)	1549-9642
ISSN	1549-9634
DOI	10.1016/j.nano.2022.102592
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Article ; Online: High throughput and very specific screening of anabolic-androgenic steroid adulterants in healthy foods based on stable isotope labelling and flow injection analysis-tandem mass spectrometry with simultaneous monitoring proton adduct ions and chloride adduct ions.

Chen, Di / Wang, Zi-Han / Cui, Wei-Qi / Zhang, Jing-Xian / Zhang, Jun-Wei / Wu, De-Qiao / Wang, Zi-Yue / Yu, Xin-Rui / Luo, Yan-Bo / Hussain, Dilshad / Xu, Xia

Journal of chromatography. A

2022 Volume 1667, Page(s) 462891

Abstract: In this work, a stable isotope labelling-flow injection analysis-tandem mass spectrometry (SIL-FIA-MS/MS) with simultaneous monitoring [M+H] ...

Abstract	In this work, a stable isotope labelling-flow injection analysis-tandem mass spectrometry (SIL-FIA-MS/MS) with simultaneous monitoring [M+H]
MeSH term(s)	Chlorides ; Chromatography, High Pressure Liquid ; Flow Injection Analysis ; Isotope Labeling ; Protons ; Steroids/chemistry ; Tandem Mass Spectrometry
Chemical Substances	Chlorides ; Protons ; Steroids
Language	English
Publishing date	2022-02-10
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1171488-8
ISSN	1873-3778 ; 0021-9673
ISSN (online)	1873-3778
ISSN	0021-9673
DOI	10.1016/j.chroma.2022.462891
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Article ; Online: Combining Fruquintinib and Doxorubicin in Size-Converted Nano-Drug Carriers for Tumor Therapy.

Zhang, Nan / Xin, Xiangying / Feng, Nannan / Wu, Deqiao / Zhang, Junwei / Yu, Tong / Jiang, Qianqian / Gao, Ming / Yang, Hui / Zhao, Siyuan / Tian, Qingfeng / Zhang, Zhenzhong

ACS biomaterials science & engineering

2022 Volume 8, Issue 5, Page(s) 1907–1920

Abstract: Single-modality tumor therapy confronts many challenges, such as incomplete tumor ablation, tumor metastasis, and limited tumor tissue penetration. Combination therapy simultaneously achieves deep drug delivery to fully exert synergistic effects and has ... ...

Abstract	Single-modality tumor therapy confronts many challenges, such as incomplete tumor ablation, tumor metastasis, and limited tumor tissue penetration. Combination therapy simultaneously achieves deep drug delivery to fully exert synergistic effects and has received increasing attention. Herein, based on the excellent efficacy of anti-angiogenesis therapy combined with chemotherapy and the specific size of the poly-amidoamine dendrimer (PAMAM), we developed a pH-triggered size-converted nano-drug delivery system to co-deliver fruquintinib (FRU) and doxorubicin (DOX). This study used cyclic Arg-Gly-Asp (cRGD) as the target, pH-responsive liposomes (PRLs), and PAMAM as the drug carrier. The FRU and DOX-loaded small-particle-size complex polyamide-amine-doxorubicin (PD) was encapsulated into PRLs with the target to construct a size-converted nano-drug delivery system, PRL-PD/FRU-cRGD. This nanoparticle (∼120 nm) actively targeted tumor tissues and used the acidic microenvironment outside tumor cells to release FRU and small-particle-size complex PD (∼15 nm), enabling the conversion of large-size nanoparticles to small-size nanoparticles and resulting in efficient tumor accumulation. In addition, the released PD could realize the deep delivery of DOX, showing efficient deep tumor penetration and further enhancing the tumor-suppressing effect. The results of in vivo and in vitro experiments showed that PRL-PD/FRU-cRGD exhibited the excellent synergistic effects of anti-angiogenesis therapy combined with chemotherapy and effectively inhibited tumor cell proliferation and metastasis, thereby achieving efficient tumor therapy. Thus, PRL-PD/FRU-cRGD shows great potential for combined tumor therapy.
MeSH term(s)	Benzofurans ; Doxorubicin/therapeutic use ; Drug Carriers ; Humans ; Liposomes ; Nanoparticles/therapeutic use ; Neoplasms/drug therapy ; Quinazolines ; Tumor Microenvironment
Chemical Substances	Benzofurans ; Drug Carriers ; Liposomes ; Quinazolines ; HMPL-013 (49DXG3M5ZW) ; Doxorubicin (80168379AG)
Language	English
Publishing date	2022-04-28
Publishing country	United States
Document type	Journal Article
ISSN	2373-9878
ISSN (online)	2373-9878
DOI	10.1021/acsbiomaterials.1c01606
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Article: High throughput and very specific screening of anabolic-androgenic steroid adulterants in healthy foods based on stable isotope labelling and flow injection analysis-tandem mass spectrometry with simultaneous monitoring proton adduct ions and chloride adduct ions

Chen, Di / Wang, Zi-Han / Cui, Wei-Qi / Zhang, Jing-Xian / Zhang, Jun-Wei / Wu, De-Qiao / Wang, Zi-Yue / Yu, Xin-Rui / Luo, Yan-Bo / Hussain, Dilshad / Xu, Xia

Journal of chromatography. 2022 Mar. 29, v. 1667

2022

Abstract: In this work, a stable isotope labelling-flow injection analysis-tandem mass spectrometry (SIL-FIA-MS/MS) with simultaneous monitoring [M+H]⁺ and [M+Cl]⁻ method was developed for very specific and high throughput screening of anabolic-androgenic steroids ...

Abstract	In this work, a stable isotope labelling-flow injection analysis-tandem mass spectrometry (SIL-FIA-MS/MS) with simultaneous monitoring [M+H]⁺ and [M+Cl]⁻ method was developed for very specific and high throughput screening of anabolic-androgenic steroids (AAS) illegally added to healthy foods. Initially, a simple centrifugation step was carried out for liquid samples, and for solid samples, a solid-liquid extraction step was conducted. Afterwards, batch chemical derivatization was carried out. After adding a certain amount of ¹³C₆-3-NPH labelled AAS standards as the internal standards, it can be directly transferred for FIA-MS/MS analysis based on the no MS response characteristics of 3-NPH. The 3-NPH labelled AAS showed dual-polarity property, observing chloride adduct ion ([M+Cl]⁻) in negative ion mode and proton adduct ion ([M+H]⁺) in positive ion mode. The average time cost for pretreatment of each sample was less than 1 min by carrying out batch processing. The subsequent FIA-MS/MS detection enabled rapid and high throughput detection. The addition of ¹³C₆-3-NPH-labelled AAS as internal standards can correct the matrix effect to achieve accurate quantitative analysis. The detection sensitivity was also improved by 2–5 folds after 3-NPH labelling. The limits of detection (LODs) in positive MRM mode were in ranges of 0.1–0.3 ng/mL. The validated method with simultaneous monitoring [M+H]⁺ and [M+Cl]⁻ was validated in the range of 6.0–1000 ng/mL with the linear coefficient (R²) greater than 0.997. Satisfactory recoveries were found to be in ranges of 93.0–108.7%. The intra-day and inter-day RSDs were in the range of 3.5–9.9% and 5.1–14.1%, respectively. No changes in detection sensitivity of the mass spectrometry and no carry-over effects were found after numerous consecutive injections of AAS derivates. Compared with previously reported methods, the proposed method proved accurate, very specific, high throughput with good sensitivity.
Keywords	centrifugation ; chromatography ; derivatization ; detection limit ; liquids ; quantitative analysis ; stable isotopes
Language	English
Dates of publication	2022-0329
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	218139-3
ISSN	0021-9673 ; 0378-4355 ; 0376-737X
ISSN	0021-9673 ; 0378-4355 ; 0376-737X
DOI	10.1016/j.chroma.2022.462891
Database	NAL-Catalogue (AGRICOLA)

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