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  1. Book ; Online: DNA-Correcting Codes in DNA Storage Systems

    Wu, Huawei

    2023  

    Abstract: In [1], the authors proposed a new model of DNA storage system that integrates all three steps of retrieval and introduced the concept of DNA-correcting codes, which guarantees that the output of the storage system can be decoded to the original data. ... ...

    Abstract In [1], the authors proposed a new model of DNA storage system that integrates all three steps of retrieval and introduced the concept of DNA-correcting codes, which guarantees that the output of the storage system can be decoded to the original data. They also gave necessary and sufficient conditions for DNA-correcting codes when the data part is free of errors. In this paper, we generalize their results to the general case.
    Keywords Computer Science - Information Theory
    Publishing date 2023-11-16
    Publishing country us
    Document type Book ; Online
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  2. Article: Molecular cloning, and optimized production and characterization of recombinant cyclodextrin glucanotransferase from

    Liu, Zhenyang / Wu, Guogan / Wu, Huawei

    3 Biotech

    2022  Volume 12, Issue 3, Page(s) 58

    Abstract: Cyclodextrin glucosyltransferase (CGTase) is an enzyme which degrades starch to produce cyclodextrins (CDs). In this study, the β-CGTase producing strain T1 was identified as : Supplementary information: The online version contains supplementary ... ...

    Abstract Cyclodextrin glucosyltransferase (CGTase) is an enzyme which degrades starch to produce cyclodextrins (CDs). In this study, the β-CGTase producing strain T1 was identified as
    Supplementary information: The online version contains supplementary material available at 10.1007/s13205-022-03111-8.
    Language English
    Publishing date 2022-02-05
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2600522-0
    ISSN 2190-5738 ; 2190-572X
    ISSN (online) 2190-5738
    ISSN 2190-572X
    DOI 10.1007/s13205-022-03111-8
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  3. Article: Molecular cloning, and optimized production and characterization of recombinant cyclodextrin glucanotransferase from Bacillus sp. T1

    Liu, Zhenyang / Wu, Guogan / Wu, Huawei

    3 Biotech. 2022 Mar., v. 12, no. 3

    2022  

    Abstract: Cyclodextrin glucosyltransferase (CGTase) is an enzyme which degrades starch to produce cyclodextrins (CDs). In this study, the β-CGTase producing strain T1 was identified as Bacillus sp. by its morphological characteristics and 16S rDNA sequence ... ...

    Abstract Cyclodextrin glucosyltransferase (CGTase) is an enzyme which degrades starch to produce cyclodextrins (CDs). In this study, the β-CGTase producing strain T1 was identified as Bacillus sp. by its morphological characteristics and 16S rDNA sequence analysis. The cgt-T1 gene was cloned and expressed in Escherichia coli. CGTase-T1 was purified by Ni-nitrilotriacetic acid agarose column and the molecular weight was determined as approximately 75 kDa using SDS-PAGE analysis. For the expression of soluble proteins, the optimal induction conditions were 10 h at 25 °C with OD₆₀₀ at 0.8. The purified CGTase-T1 exhibited maximum activity with an optimal pH and temperature of 6.0 and 65 °C. The enzyme was stable in a pH range of 7.0–10.0, retaining over 85% relative activity for 1 h. CGTase-T1 activity can be significantly enhanced by adding 1 mM Ba²⁺. Using a soluble starch substrate, the kinetic parameters were revealed with KM and kcₐₜ/KM values of 2.75 mg mL⁻¹ and 1253.97 s⁻¹ mL mg⁻¹, respectively. Additionally, the four enzyme activities of CGTase-T1 were determined. The highest conversion rate to CDs (40.9%) was achieved from soluble starch after 8 h of enzyme reaction, where mainly β-CD was produced (79.1% of the total CDs yield), indicating that CGTase-T1 potentially has industrial application prospect.
    Keywords Bacillus (bacteria) ; Escherichia coli ; agarose ; cyclodextrins ; cyclomaltodextrin glucanotransferase ; enzymatic reactions ; genes ; industrial applications ; molecular weight ; nucleotide sequences ; pH ; polyacrylamide gel electrophoresis ; sequence analysis ; starch ; temperature
    Language English
    Dates of publication 2022-03
    Size p. 58.
    Publishing place Springer International Publishing
    Document type Article
    ZDB-ID 2600522-0
    ISSN 2190-5738 ; 2190-572X
    ISSN (online) 2190-5738
    ISSN 2190-572X
    DOI 10.1007/s13205-022-03111-8
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  4. Article: Heterologous expression and characterization of Anaeromyces robustus xylanase and its use in bread making

    Liu, Zhenyang / Wen, Sitao / Wu, Guogan / Wu, Huawei

    European food research & technology. 2022 Sept., v. 248, no. 9

    2022  

    Abstract: The rumen of ruminants contains a variety of fungi with function of producing xylanases to break down plant cell walls. In this study, a new glycoside hydrolase family 10 (GH10) xylanase gene ArXyn10c20 from anaerobic rumen microorganism Anaeromyces ... ...

    Abstract The rumen of ruminants contains a variety of fungi with function of producing xylanases to break down plant cell walls. In this study, a new glycoside hydrolase family 10 (GH10) xylanase gene ArXyn10c20 from anaerobic rumen microorganism Anaeromyces robustus was successfully synthesized and expressed in Pichia pastoris GS115, with a protein molecular weight of approximately 42 kDa. The optimum pH and temperature for ArXyn10c20 were 5.5 and 40 ℃. ArXyn10c20 was stable in the pH range 5.0–9.0, retaining over 75.0% relative activity for 1 h. The activity of recombinant xylanase was significantly enhanced by 1 mM Cu²⁺. The products of ArXyn10c20 hydrolysis of beechwood xylan were xylobiose, xylotriose and xylotetraose by thin-layer chromatography analysis. In food applications, ArXyn10c20 can significantly improve the quality of dough and bread. With the addition of 420 U of ArXyn10c20 per 100 g flour, the hardness, gumminess and chewiness of the bread decreased by 42.2%, 45.3%, and 55.4%, respectively, and the reducing sugar increased by 18.7%. The new discovered xylanase ArXyn10c20 has great potential in food industry.
    Keywords Komagataella pastoris ; breads ; chewiness ; dough ; flour ; food industry ; food research ; genes ; glycosides ; hardness ; heterologous gene expression ; hydrolysis ; molecular weight ; pH ; temperature ; thin layer chromatography ; xylan ; xylanases
    Language English
    Dates of publication 2022-09
    Size p. 2311-2324.
    Publishing place Springer Berlin Heidelberg
    Document type Article
    ZDB-ID 1359456-4
    ISSN 1431-4630 ; 1438-2377
    ISSN 1431-4630 ; 1438-2377
    DOI 10.1007/s00217-022-04047-2
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  5. Book ; Online: Circular External Difference Families

    Wu, Huawei / Yang, Jing / Feng, Keqin

    Construction and Non-Existence

    2023  

    Abstract: The circular external difference family and its strong version, which themselves are of independent combinatorial interest, were proposed as variants of the difference family to construct new unconditionally secure non-malleable threshold schemes. In ... ...

    Abstract The circular external difference family and its strong version, which themselves are of independent combinatorial interest, were proposed as variants of the difference family to construct new unconditionally secure non-malleable threshold schemes. In this paper, we present new results regarding the construction and non-existence of (strong) circular external difference families, thereby solving several open problems on this topic.
    Keywords Mathematics - Combinatorics ; Computer Science - Information Theory
    Publishing date 2023-10-16
    Publishing country us
    Document type Book ; Online
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  6. Book ; Online: The Weight Distributions of Two Classes of Linear Codes From Perfect Nonlinear Functions

    Wu, Huawei / Yang, Jing / Feng, Keqin

    2023  

    Abstract: In this paper, we employ general results on the value distributions of perfect nonlinear functions from $\mathbb{F}_{p^m}$ to $\mathbb{F}_p$ together with a specific group action to give a unified approach to determining the weight distributions of two ... ...

    Abstract In this paper, we employ general results on the value distributions of perfect nonlinear functions from $\mathbb{F}_{p^m}$ to $\mathbb{F}_p$ together with a specific group action to give a unified approach to determining the weight distributions of two classes of linear codes over $\mathbb{F}_p$ constructed from perfect nonlinear functions, where $p$ is an odd prime number and $m\in\mathbb{N}_+$.
    Keywords Computer Science - Information Theory
    Publishing date 2023-06-10
    Publishing country us
    Document type Book ; Online
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  7. Article ; Online: Research on damping performance and strength of the composite laminate.

    Zhang, Bao / Li, Zhi / Wu, Huawei / Nie, Jinquan

    Scientific reports

    2021  Volume 11, Issue 1, Page(s) 18281

    Abstract: In this paper, the influence of E-glass fiber volume fraction and laying angle on the damping and strength of composite laminates was comprehensively analyzed. By increasing the fiber laying angle and reducing the glass fiber volume fraction, the damping ...

    Abstract In this paper, the influence of E-glass fiber volume fraction and laying angle on the damping and strength of composite laminates was comprehensively analyzed. By increasing the fiber laying angle and reducing the glass fiber volume fraction, the damping capacity of the composite laminate was increased, but the tensile strength of the laminate was reduced; By reducing the fiber laying angle and increasing the glass fiber volume fraction, the tensile strength of the composite laminate was increased, but the damping characteristics of the laminate was reduced. In addition, in the damping experiment of composite laminates, in order to avoid the influence of external damping sources, the vacuum non-contact damping test method was adopted in this paper, and the influence of air damping on the damping experiment results of composite laminates was comparatively analyzed. The results of comparative experiments showed that air damping had a very obvious influence on the damping of composite laminates, especially when the damping of composite laminates was small, the influence of air damping would be greater.
    Language English
    Publishing date 2021-09-14
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-021-97933-w
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  8. Article ; Online: Fault Detection of Wind Turbine Electric Pitch System Based on IGWO-ERF.

    Tang, Mingzhu / Yi, Jiabiao / Wu, Huawei / Wang, Zimin

    Sensors (Basel, Switzerland)

    2021  Volume 21, Issue 18

    Abstract: It is difficult to optimize the fault model parameters when Extreme Random Forest is used to detect the electric pitch system fault model of the double-fed wind turbine generator set. Therefore, Extreme Random Forest which was optimized by improved grey ... ...

    Abstract It is difficult to optimize the fault model parameters when Extreme Random Forest is used to detect the electric pitch system fault model of the double-fed wind turbine generator set. Therefore, Extreme Random Forest which was optimized by improved grey wolf algorithm (IGWO-ERF) was proposed to solve the problems mentioned above. First, IGWO-ERF imports the Cosine model to nonlinearize the linearly changing convergence factor α to balance the global exploration and local exploitation capabilities of the algorithm. Then, in the later stage of the algorithm iteration, α wolf generates its mirror wolf based on the lens imaging learning strategy to increase the diversity of the population and prevent local optimum of the population. The electric pitch system fault detection method of the wind turbine generator set sets the generator power of the variable pitch system as the main state parameter. First, it uses the Pearson correlation coefficient method to eliminate the features with low correlation with the electric pitch system generator power. Then, the remaining features are ranked by the importance of the RF features. Finally, the top N features are selected to construct the electric pitch system fault data set. The data set is divided into a training set and a test set. The training set is used to train the proposed fault detection model, and the test set is used for testing. Compared with other parameter optimization algorithms, the proposed method has lower FNR and FPR in the electric pitch system fault detection of the wind turbine generator set.
    Language English
    Publishing date 2021-09-16
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2052857-7
    ISSN 1424-8220 ; 1424-8220
    ISSN (online) 1424-8220
    ISSN 1424-8220
    DOI 10.3390/s21186215
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  9. Article: Biochemical characterization of a GH10 xylanase from the anaerobic rumen fungus

    Wen, Sitao / Wu, Guogan / Wu, Huawei

    3 Biotech

    2021  Volume 11, Issue 9, Page(s) 406

    Abstract: Anaeromyces ... ...

    Abstract Anaeromyces robustus
    Language English
    Publishing date 2021-08-11
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2600522-0
    ISSN 2190-5738 ; 2190-572X
    ISSN (online) 2190-5738
    ISSN 2190-572X
    DOI 10.1007/s13205-021-02956-9
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  10. Article: Strong adsorption of phosphate by amorphous lanthanum carbonate nano-adsorbents.

    Lu, Yifan / Wu, Huawei / Xia, Yan / Huang, Mei

    Water science and technology : a journal of the International Association on Water Pollution Research

    2021  Volume 83, Issue 7, Page(s) 1605–1618

    Abstract: Phosphorus removal is a crucial aspect of controlling water pollution and eutrophication. In this study, the preparation of lanthanum carbonate (LC) nano-adsorbents for the efficient removal of phosphate (P) from water and wastewater was investigated. ... ...

    Abstract Phosphorus removal is a crucial aspect of controlling water pollution and eutrophication. In this study, the preparation of lanthanum carbonate (LC) nano-adsorbents for the efficient removal of phosphate (P) from water and wastewater was investigated. Results from XRD, SEM and Zeta potential analyses revealed that addition of magnesium ions and adjustment of the reaction temperature could control the morphology and microstructure of LC. Effects of initial pH, adsorbent dosage, contact time, and the water matrix on P adsorption were investigated. Batch adsorption experiments revealed that LC showed strong performance on P removal over a wide pH range (3.0 to 11.0). The kinetic data followed a pseudo-second-order model, and equilibrium data were well fitted by the Langmuir model with a maximum adsorption capacity of 112.9 mg P/g. Adsorption thermodynamics showed that the adsorption process was exothermic and spontaneous. Results of a monolayer model for single adsorption indicated that P could completely interact with two or more functional groups from the LC surface. In the presence of competing ions (F
    MeSH term(s) Adsorption ; Hydrogen-Ion Concentration ; Kinetics ; Lanthanum ; Phosphates ; Waste Water ; Water Pollutants, Chemical/analysis
    Chemical Substances Phosphates ; Waste Water ; Water Pollutants, Chemical ; lanthanum carbonate (490D9F069T) ; Lanthanum (6I3K30563S)
    Language English
    Publishing date 2021-04-09
    Publishing country England
    Document type Journal Article
    ZDB-ID 764273-8
    ISSN 1996-9732 ; 0273-1223
    ISSN (online) 1996-9732
    ISSN 0273-1223
    DOI 10.2166/wst.2021.086
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