LIVIVO - Search results -

Search results

Result 1 - 10 of total 31

Search options

Article ; Online: Recent progress of atmospheric and room-temperature plasma as a new and promising mutagenesis technology.

Li, Dongao / Shen, Jie / Ding, Qiang / Wu, Jinyong / Chen, Xiangsong

2024 Volume 42, Issue 3, Page(s) e3991

Abstract: At present, atmospheric and room-temperature plasma (ARTP) is regarded as a new and powerful mutagenesis technology with the advantages of environment-friendliness, operation under mild conditions, and fast mutagenesis speed. Compared with traditional ... ...

Abstract	At present, atmospheric and room-temperature plasma (ARTP) is regarded as a new and powerful mutagenesis technology with the advantages of environment-friendliness, operation under mild conditions, and fast mutagenesis speed. Compared with traditional mutagenesis strategies, ARTP is used mainly to change the structure of microbial DNA, enzymes, and proteins through a series of physical, chemical, and electromagnetic effects with the organisms, leading to nucleotide breakage, conversion or inversion, causing various DNA damages, so as to screen out the microbial mutants with better biological characteristics. As a result, in recent years, ARTP mutagenesis and the combination of ARTP with traditional mutagenesis have been widely used in microbiology, showing great potential for application. In this review, the recent progress of ARTP mutagenesis in different application fields and bottlenecks of this technology are systematically summarized, with a view to providing a theoretical basis and technical support for better application. Finally, the outlook of ARTP mutagenesis is presented, and we identify the challenges in the field of microbial mutagenesis by ARTP.
MeSH term(s)	Temperature ; Mutagenesis ; DNA Damage ; DNA
Chemical Substances	DNA (9007-49-2)
Language	English
Publishing date	2024-03-15
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	283643-9
ISSN	1099-0844 ; 0263-6484
ISSN (online)	1099-0844
ISSN	0263-6484
DOI	10.1002/cbf.3991
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1994: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾

Article ; Online: Wild birds-the sentinel of antibiotic resistance for urban river: Study on egrets and Jinjiang river in Chengdu, China.

Lin, Yufei / Zhang, Lihua / Wu, Jinyong / Yang, Kun

Environmental research

2022 Volume 216, Issue Pt 3, Page(s) 114566

Abstract: Antibiotic resistance has become a comprehensive and complicated environmental problem. It is of great importance to effectively determine the abundance of various antibiotic resistance genes (ARGs) in the environment. Here, we attempted to find a ... ...

Abstract	Antibiotic resistance has become a comprehensive and complicated environmental problem. It is of great importance to effectively determine the abundance of various antibiotic resistance genes (ARGs) in the environment. Here, we attempted to find a practical method for monitoring environmental antibiotic resistance. The results of culture-based analysis of antibiotic resistance and metagenomic sequencing indicate that egrets inhabiting along the urban river (Jinjiang River) can be used as the sentinel of environmental antibiotic resistance. The antibiotic resistance in the environment fluctuated with time, while that in the wild bird was relatively stable. The network analysis based on metagenomic sequencing data gave the co-occurrence pattern of ARGs. The overall situation of the antibiotic resistance in the river was determined by quantifying several module hub genes of the co-occurrence network in river sediments. The temporal and spatial distribution of ARGs in Jinjiang River is highly correlated with that of human gut-specific bacteriophage (crAssphage), which indicates that one main source of the antibiotic resistance in the river is likely to be municipal sewage. The mobility potential of ARGs varying among different niches suggests the transmission direction of antibiotic resistance in the environment.
MeSH term(s)	Animals ; Anti-Bacterial Agents/pharmacology ; Birds/microbiology ; China ; Drug Resistance, Microbial/genetics ; Genes, Bacterial ; Metagenomics ; Rivers ; Environmental Monitoring
Chemical Substances	Anti-Bacterial Agents
Language	English
Publishing date	2022-10-20
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	205699-9
ISSN	1096-0953 ; 0013-9351
ISSN (online)	1096-0953
ISSN	0013-9351
DOI	10.1016/j.envres.2022.114566
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 726: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article: Supplementation of

Jin, Long / Dang, Hongyang / Wu, Jinyong / Yuan, Lixia / Chen, Xiangsong / Yao, Jianming

Microorganisms

2023 Volume 11, Issue 2

Abstract: ... The ... ...

Abstract	The probiotic
Language	English
Publishing date	2023-01-19
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms11020264
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: High-Yield Synthesis of Lacto-

Liao, Yingxue / Lao, Caiwen / Wu, Jinyong / Yuan, Lixia / Xu, Yanyi / Jin, Weijian / Sun, Jian / Zhang, Qiang / Chen, Xiangsong / Yao, Jianming

Journal of agricultural and food chemistry

2024 Volume 72, Issue 10, Page(s) 5325–5338

Abstract: Lacto- ...

Abstract	Lacto-
MeSH term(s)	Humans ; Glycerol/metabolism ; Escherichia coli/metabolism ; Oligosaccharides/metabolism ; Milk, Human/metabolism
Chemical Substances	lacto-N-neotetraose (BY63N40B1L) ; Glycerol (PDC6A3C0OX) ; Oligosaccharides
Language	English
Publishing date	2024-01-26
Publishing country	United States
Document type	Journal Article
ZDB-ID	241619-0
ISSN	1520-5118 ; 0021-8561
ISSN (online)	1520-5118
ISSN	0021-8561
DOI	10.1021/acs.jafc.3c08239
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1172: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Gamma-Aminobutyric Acid-Producing Levilactobacillus brevis Strains as Probiotics in Litchi Juice Fermentation

Jin, Yiwen / Wu, Jinyong / Hu, Dan / Li, Jun / Zhu, Weiwei / Yuan, Lixia / Chen, Xiangsong / Yao, Jianming

Foods. 2023 Jan. 08, v. 12, no. 2

2023

Abstract: Levilactobacillus brevis strains can be isolated from traditional Chinese pickles and used as the starter cultures to improve the nutritional profiles of fermented juices. Three L. brevis strains (LBG-29, LBG-24, LBD–14) that produce high levels of gamma- ...

Abstract	Levilactobacillus brevis strains can be isolated from traditional Chinese pickles and used as the starter cultures to improve the nutritional profiles of fermented juices. Three L. brevis strains (LBG-29, LBG-24, LBD–14) that produce high levels of gamma-aminobutyric acid (GABA; >300 mg/L) were isolated from traditional Chinese pickles. The strains showed tolerance to low pH and high bile salts and exhibited safety in vitro. Litchi juice was fermented using each strain at 37 °C for 48 h. The litchi juice was determined to be a good substrate for fermentation as the process enhanced its functional profile. Overall, cell vitality increased (above 8.7 log₁₀ CFU/mL), the antioxidant activities of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric ion-reducing antioxidant power (FRAP) were significantly increased, and the antioxidant capacity of the 2,2′-amino-di(3-ethyl-benzothiazoline sulphonic acid-6)ammonium salt (ABTS) was decreased. There was also a significant increase in the GABA and acetic acid content after LBG-29 and LBG-24 fermentation. It was thus determined that the LBG-29 and LBG-24 strains could be used to improve beverage functionality and aid in the development of new products. This is the first report of litchi fermentation using L. brevis as a starter culture. Further research is required to elucidate the functional benefits for the human body and the nutritional and functional properties during its shelf life.
Keywords	2,2-diphenyl-1-picrylhydrazyl ; Litchi ; acetic acid ; ammonium salts ; antioxidant activity ; antioxidants ; beverages ; bile ; fermentation ; gamma-aminobutyric acid ; humans ; juices ; pH ; probiotics ; shelf life ; starter cultures
Language	English
Dates of publication	2023-0108
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2704223-6
ISSN	2304-8158
ISSN	2304-8158
DOI	10.3390/foods12020302
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: Exploration on wastewater-based epidemiology of SARS-CoV-2: Mimic relative quantification with endogenous biomarkers as internal reference

Tang, Langjun / Wu, Jinyong / Liu, Rui / Feng, Zhongxi / Zhang, Yanan / Zhao, Yingzhe / Li, Yonghong / Yang, Kun

Heliyon. 2023 May, v. 9, no. 5 p.e15705-

2023

Abstract: Wastewater-based epidemiology has become a powerful surveillance tool for monitoring the pandemic of COVID-19. Although it is promising to quantitatively correlate the SARS-CoV-2 RNA concentration in wastewater with the incidence of community infection, ... ...

Abstract	Wastewater-based epidemiology has become a powerful surveillance tool for monitoring the pandemic of COVID-19. Although it is promising to quantitatively correlate the SARS-CoV-2 RNA concentration in wastewater with the incidence of community infection, there is still no consensus on whether the viral nucleic acid concentration in sewage should be normalized against the abundance of endogenous biomarkers and which biomarker should be used as a reference for the normalization. Here, several candidate endogenous reference biomarkers for normalization of SARS-CoV-2 signal in municipal sewage were evaluated. The human fecal indicator virus (crAssphage) is a promising candidate of endogenous reference biomarker for data normalization of both DNA and RNA viruses for its intrinsic viral nature and high and stable content in sewage. Without constructing standard curves, the relative quantification of sewage viral nucleic acid against the abundance of the reference biomarker can be used to correlate with community COVID-19 incidence, which was proved via mimic experiments by spiking pseudovirus of different concentrations in sewage samples. Dilution of pseudovirus-seeded wastewater did not affect the relative abundance of viral nucleic acid, demonstrating that relative quantification can overcome the sewage dilution effects caused by the greywater input, precipitation and/or groundwater infiltration. The process of concentration, recovery and detection of the endogenous biomarker was consistent with that of SARS-CoV-2 RNA. Thus, it is necessary to co-quantify the endogenous biomarker because it can be not only an internal reference for data normalization, but also a process control.
Keywords	COVID-19 infection ; DNA ; RNA ; Severe acute respiratory syndrome coronavirus 2 ; biomarkers ; greywater ; groundwater ; humans ; monitoring ; pandemic ; process control ; sewage ; viruses ; Wastewater-based epidemiology ; Endogenous biomarker ; SARS-CoV-2 ; crAssphage ; RT-qPCR
Language	English
Dates of publication	2023-05
Publishing place	Elsevier Ltd
Document type	Article ; Online
Note	Use and reproduction
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2023.e15705
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article: Effect of isomaltose oligosaccharide on the freeze-drying process and shelf-life of Pediococcus pentosaceus

Hu, Dan / Wu, Jinyong / Li, Jun / Jin, Long / Chen, Xiangsong / Yao, Jianming

Lebensmittel-Wissenschaft + Technologie. 2022 June 15, v. 163

2022

Abstract: Improvement in the survival of the edible probiotic Pediococcus pentosaceus during the preparation and storage is important. In this study, the protective effects of isomaltose oligosaccharide in a protective agent (sodium glutamate, skimmed milk, and ... ...

Abstract	Improvement in the survival of the edible probiotic Pediococcus pentosaceus during the preparation and storage is important. In this study, the protective effects of isomaltose oligosaccharide in a protective agent (sodium glutamate, skimmed milk, and trehalose) on P. pentosaceus during vacuum freeze-drying and low-temperature storage were evaluated. Cell damage, and texture were evaluated using fluorescence analyses, and scanning electron microscopy, respectively, and a water activity assay was performed storage after 1 month of storage (−80, −20, 4, 25 °C). The viability of P. pentosaceus agents (24444, QK-1, MQ-1, and RQ-1) were evaluated for 9 months storage. The results showed that IMO increased 11.02% survival rate of P. pentosaceus QK-1 during the freeze-drying process, which was better than that of 24444, MQ-1, and RQ-1; the texture of the protective agent was thin, with a compact and granular cross-section. Decreased water activity (18.71–34.26%) and cell damage (8.32–10.53%), also enhanced survival rate (5.98–12.85%) were observed in the IMO-containing groups in storage. The survival rates of P. pentosaceus (QK-1, MQ-1, RQ-1) direct-injection agents (9 months) exceeded 90% under gastrointestinal satiety-mimicking conditions in vitro. Our data indicate that IMO has a good protective effect on the freeze-drying and storage processes of P. pentosaceus.
Keywords	Pediococcus pentosaceus ; cold storage ; fluorescence ; freeze drying ; gastrointestinal system ; isomaltose ; monosodium glutamate ; probiotics ; protective effect ; shelf life ; survival rate ; texture ; trehalose ; viability ; water activity
Language	English
Dates of publication	2022-0615
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	241369-3
ISSN	0460-1173 ; 0023-6438
ISSN	0460-1173 ; 0023-6438
DOI	10.1016/j.lwt.2022.113583
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 2634: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Protein engineering of CMP kinases to improve thermal stability and resultant production of 3′-sialyllactose

Wang, Jingjing / Wu, Jinyong / Li, Zhongkui / Chen, Xiangsong / Liu, Weiwei / Yao, Jianming

Biotechnology & Biotechnological Equipment. 2022 Dec. 31, v. 36, no. 1, p. 433-441

2022 , Page(s) 433–441

Abstract: Protein engineering is an effective method to improve the thermal stability of biological macromolecules. Cytidine monophosphate kinase (CMK) is a key enzyme involved in nucleotide metabolism and participates in the production of cytidine triphosphate. ... ...

Abstract	Protein engineering is an effective method to improve the thermal stability of biological macromolecules. Cytidine monophosphate kinase (CMK) is a key enzyme involved in nucleotide metabolism and participates in the production of cytidine triphosphate. It has previously been confirmed that CMK shows low thermal stability. This study aimed to enhance the thermal stability of CMK. Herein, we first analyzed native CMK using PoPMuSiC. Thereafter, we constructed single point mutants (Q30G, P62R, E85I, D148W and K165L) and multipoint mutants of CMK with higher thermal stability using site-directed mutagenesis. The thermal stability of the mutant Q30G/E85I/K165L was observed to be 1.2-fold higher than that of the wild type. The mutants were then allowed to catalyse 3′-sialyllactose (3′-SL) synthesis. After 51.72 mmol/L N-acetylneuraminic acid (sialic acid) was consumed in a 25 mL reaction system and 50.67 mmol/L 3′-SL was obtained, a decrease of 2 h was recorded in the reaction time, whereas the yield rate increased by 33.6%. The results suggest that the mutant Q30G/E85I/K165L can improve the thermal stability of CMK and its catalytic efficiency for 3′-SL synthesis. These findings are crucial for various applications involving enzyme conversion.
Keywords	biotechnology ; catalytic activity ; cytidine monophosphate ; cytidine triphosphate ; equipment ; metabolism ; mutants ; phosphotransferases (kinases) ; sialic acid ; site-directed mutagenesis ; thermal stability ; protein engineering ; cytidine monophosphate kinase ; 3′-sialyllactose ; catalytic efficiency
Language	English
Dates of publication	2022-1231
Size	p. 433-441
Publishing place	Taylor & Francis
Document type	Article ; Online
ZDB-ID	1214963-9
ISSN	1314-3530 ; 0205-2067 ; 1310-2818
ISSN (online)	1314-3530
ISSN	0205-2067 ; 1310-2818
DOI	10.1080/13102818.2022.2095302
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 6265: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Reversing Antibiotic Resistance Caused by Mobile Resistance Genes of High Fitness Cost.

Wu, Jinyong / Dong, Xiaohong / Zhang, Lihua / Lin, Yufei / Yang, Kun

mSphere

2021 Volume 6, Issue 3, Page(s) e0035621

Abstract: The reversibility of antibiotic resistance is theoretically attractive due to the prospect of restoring the clinical potency of antibiotics. It is important to find out the factors that affect the reversibility of antibiotic resistance. Here, ... ...

Abstract	The reversibility of antibiotic resistance is theoretically attractive due to the prospect of restoring the clinical potency of antibiotics. It is important to find out the factors that affect the reversibility of antibiotic resistance. Here, an
MeSH term(s)	Anti-Bacterial Agents/pharmacology ; Drug Resistance, Bacterial/genetics ; Escherichia coli/drug effects ; Escherichia coli/genetics ; Escherichia coli Proteins/genetics ; Genetic Fitness ; Plasmids ; Whole Genome Sequencing
Chemical Substances	Anti-Bacterial Agents ; Escherichia coli Proteins ; MCR-1 protein, E coli
Language	English
Publishing date	2021-06-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	2379-5042
ISSN (online)	2379-5042
DOI	10.1128/mSphere.00356-21
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Weizmannia coagulans

Jin, Long / Dang, Hongyang / Wu, Jinyong / Yuan, Lixia / Chen, Xiangsong / Yao, Jianming

Nutrients

2022 Volume 14, Issue 19

Abstract: 1) Background: Ellagic acid (EA) acts as a product of gut microbiota transformation to prevent insulin resistance, which is limited by high-fat diet (HFD)-induced dysbiosis. The aim of this study was to investigate the synergistic effects and mechanisms ...

Abstract	(1) Background: Ellagic acid (EA) acts as a product of gut microbiota transformation to prevent insulin resistance, which is limited by high-fat diet (HFD)-induced dysbiosis. The aim of this study was to investigate the synergistic effects and mechanisms of supplementation with the probiotic
MeSH term(s)	Animals ; Autophagy ; Diet, High-Fat/adverse effects ; Ellagic Acid/metabolism ; Ellagic Acid/pharmacology ; Gastrointestinal Microbiome ; Insulin Resistance ; Liver/metabolism ; Mice ; Mice, Inbred C57BL
Chemical Substances	Ellagic Acid (19YRN3ZS9P)
Language	English
Publishing date	2022-10-09
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2518386-2
ISSN	2072-6643 ; 2072-6643
ISSN (online)	2072-6643
ISSN	2072-6643
DOI	10.3390/nu14194206
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

To top

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito